{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# PubMed数据库挖掘文献信息"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 加载包"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "from Bio import Entrez\n",
    "from Bio import Medline\n",
    "import ipywidgets as widgets\n",
    "from IPython.display import display\n",
    "import pandas as pd\n",
    "import numpy as np"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "# 设置交互的5个模块\n",
    "accordion = widgets.Accordion(\n",
    "    children=[\n",
    "        widgets.Text(value='\"Autophagy\"[Mesh]',\n",
    "                     placeholder='请输入检索词',\n",
    "                     description='检索词:',\n",
    "                     disabled=False),\n",
    "        widgets.BoundedIntText(\n",
    "            value=300,\n",
    "            min=1,\n",
    "            max=999999,\n",
    "            step=1,\n",
    "            description='数量:',\n",
    "            disabled=False),\n",
    "        widgets.Button(description=\"点击按钮运行\"),\n",
    "        widgets.Button(description=\"点击按钮运行\"),\n",
    "        widgets.Button(description=\"点击按钮运行\")\n",
    "    ])\n",
    "\n",
    "accordion.set_title(0, '请输入检索词')\n",
    "accordion.set_title(1, '请输入检索文献的数量')\n",
    "accordion.set_title(2, '绘制词云图')\n",
    "accordion.set_title(3, '绘制基因分布图')\n",
    "accordion.set_title(4, '绘制疾病分布图')\n",
    "# 设置Button的响应事件\n",
    "output = widgets.Output()\n",
    "def on_button_click_wordcloud(x):\n",
    "    with output:\n",
    "        print(\"请稍等\")\n",
    "        get_wordcloud()\n",
    "    with output:\n",
    "        print(\"\\n已结束\")\n",
    "\n",
    "def on_button_click_gene(x):\n",
    "    with output:\n",
    "        print(\"请稍等\")\n",
    "        get_gene()\n",
    "    with output:\n",
    "        print(\"\\n已结束\")\n",
    "\n",
    "def on_button_click_disease(x):\n",
    "    with output:\n",
    "        print(\"请稍等\")\n",
    "        get_disease()\n",
    "    with output:\n",
    "        print(\"\\n已结束\")"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 利用Biopython获取文献数据"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "def get_data():\n",
    "    ### 获取PMID\n",
    "    Entrez.email = \"\"\n",
    "    handle = Entrez.esearch(db = \"pubmed\", term = accordion.children[0].value, retmax = accordion.children[1].value)\n",
    "    idlist = Entrez.read(handle)[\"IdList\"]\n",
    "    ### 通过PMID解析数据\n",
    "    handle = Entrez.efetch(db=\"pubmed\", id=idlist, rettype=\"medline\", retmode=\"text\")\n",
    "    records = Medline.parse(handle)\n",
    "    records = list(records)\n",
    "    data = []\n",
    "    for i in range(len(records)):\n",
    "        if 'AB' in records[i].keys():\n",
    "            data.append(records[i]['AB'])\n",
    "    return data"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 绘制词云图"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "def Vega(spec):\n",
    "    bundle = {}\n",
    "    bundle['application/vnd.vega.v5+json'] = spec\n",
    "    display(bundle, raw=True)\n",
    "    \n",
    "def get_wordcloud():\n",
    "    # 获取abstract数据\n",
    "    data = get_data()\n",
    "    # 绘图\n",
    "    Vega({\n",
    "        \"$schema\": \"https://vega.github.io/schema/vega/v5.json\",\n",
    "        \"width\": 1000,\n",
    "        \"height\": 500,\n",
    "        \"padding\": 0,\n",
    "        \"data\": [\n",
    "            {\n",
    "                \"name\": \"table\",\n",
    "                \"values\": data,\n",
    "                \"transform\": [\n",
    "                    {\n",
    "                        \"type\": \"countpattern\",\n",
    "                        \"field\": \"data\",\n",
    "                        \"case\": \"upper\",\n",
    "                        \"pattern\": \"[\\\\w']{3,}\",\n",
    "                        \"stopwords\": \"(i|me|my|myself|we|us|our|ours|ourselves|you|your|yours|yourself|yourselves|he|him|his|himself|she|her|hers|herself|it|its|itself|they|them|their|theirs|themselves|what|which|who|whom|whose|this|that|these|those|am|is|are|was|were|be|been|being|have|has|had|having|do|does|did|doing|will|would|should|can|could|ought|i'm|you're|he's|she's|it's|we're|they're|i've|you've|we've|they've|i'd|you'd|he'd|she'd|we'd|they'd|i'll|you'll|he'll|she'll|we'll|they'll|isn't|aren't|wasn't|weren't|hasn't|haven't|hadn't|doesn't|don't|didn't|won't|wouldn't|shan't|shouldn't|can't|cannot|couldn't|mustn't|let's|that's|who's|what's|here's|there's|when's|where's|why's|how's|a|an|the|and|but|if|or|because|as|until|while|of|at|by|for|with|about|against|between|into|through|during|before|after|above|below|to|from|up|upon|down|in|out|on|off|over|under|again|further|then|once|here|there|when|where|why|how|all|any|both|each|few|more|most|other|some|such|no|nor|not|only|own|same|so|than|too|very|say|says|said|shall)\"\n",
    "                    },\n",
    "                    {\n",
    "                        \"type\": \"formula\", \"as\": \"angle\",\n",
    "                        \"expr\": \"[-45, 0, 45][~~(random() * 3)]\"\n",
    "                    },\n",
    "                    {\n",
    "                        \"type\": \"formula\", \"as\": \"weight\",\n",
    "                        \"expr\": \"if(datum.text=='VEGA', 600, 300)\"\n",
    "                    }\n",
    "                ]\n",
    "            }\n",
    "        ],\n",
    "        \"scales\": [\n",
    "            {\n",
    "                \"name\": \"color\",\n",
    "                \"type\": \"ordinal\",\n",
    "                \"domain\": {\"data\": \"table\", \"field\": \"text\"},\n",
    "                \"range\": [\"#d5a928\", \"#652c90\", \"#939597\"]\n",
    "            }\n",
    "        ],\n",
    "        \"marks\": [\n",
    "            {\n",
    "                \"type\": \"text\",\n",
    "                \"from\": {\"data\": \"table\"},\n",
    "                \"encode\": {\n",
    "                    \"enter\": {\n",
    "                        \"text\": {\"field\": \"text\"},\n",
    "                        \"align\": {\"value\": \"center\"},\n",
    "                        \"baseline\": {\"value\": \"alphabetic\"},\n",
    "                        \"fill\": {\"scale\": \"color\", \"field\": \"text\"}\n",
    "                    },\n",
    "                    \"update\": {\n",
    "                        \"fillOpacity\": {\"value\": 1}\n",
    "                    },\n",
    "                    \"hover\": {\n",
    "                        \"fillOpacity\": {\"value\": 0.5}\n",
    "                    }\n",
    "                },\n",
    "                \"transform\": [\n",
    "                    {\n",
    "                        \"type\": \"wordcloud\",\n",
    "                        \"size\": [1000, 500],\n",
    "                        \"text\": {\"field\": \"text\"},\n",
    "                        \"rotate\": {\"field\": \"datum.angle\"},\n",
    "                        \"font\": \"Helvetica Neue, Arial\",\n",
    "                        \"fontSize\": {\"field\": \"datum.count\"},\n",
    "                        \"fontWeight\": {\"field\": \"datum.weight\"},\n",
    "                        \"fontSizeRange\": [6, 56],\n",
    "                        \"padding\": 2\n",
    "                    }\n",
    "                ]\n",
    "            }\n",
    "        ]\n",
    "    })"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 绘制基因分布图"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "def get_gene():\n",
    "    data = pd.read_csv(\"../Genes.csv\")\n",
    "    data = data[\"Genes\"]\n",
    "    data = list(data)\n",
    "    datalist = []\n",
    "    for i in data:\n",
    "        i = i.split('>',1)[1]\n",
    "        datalist.append(i)\n",
    "    datalist = np.unique(datalist, return_counts=True)\n",
    "    data = []\n",
    "    for i in range(len(datalist[0])):\n",
    "        dictionary = {}\n",
    "        dictionary['GeneID'] = datalist[0][i]\n",
    "        dictionary['Count'] = datalist[1][i]\n",
    "        data.append(dictionary)\n",
    "        \n",
    "    # 由于数值太多，因此对字典排序，选取最高的前15个数据\n",
    "    def takefifteen(x):\n",
    "        return x['Count']\n",
    "    data.sort(key=takefifteen,reverse=1)\n",
    "    data = data[0:15]\n",
    "    \n",
    "    Vega({\n",
    "        \"$schema\": \"https://vega.github.io/schema/vega/v5.json\",\n",
    "        \"width\": 1000,\n",
    "        \"height\": 500,\n",
    "        \"padding\": 5,\n",
    "        \"data\": [\n",
    "            {\n",
    "                \"name\": \"table\",\n",
    "                \"values\":data\n",
    "            }\n",
    "        ],\n",
    "        \"signals\": [\n",
    "            {\n",
    "                \"name\": \"tooltip\",\n",
    "                \"value\": {},\n",
    "                \"on\": [\n",
    "                    {\"events\": \"rect:mouseover\", \"update\": \"datum\"},\n",
    "                    {\"events\": \"rect:mouseout\",  \"update\": \"{}\"}\n",
    "                ]\n",
    "            }\n",
    "        ],\n",
    "        \"scales\": [\n",
    "            {\n",
    "                \"name\": \"xscale\",\n",
    "                \"type\": \"band\",\n",
    "                \"domain\": {\"data\": \"table\", \"field\": \"GeneID\"},\n",
    "                \"range\": \"width\",\n",
    "                \"padding\": 0.1\n",
    "            },\n",
    "            {\n",
    "                \"name\": \"yscale\",\n",
    "                \"domain\": {\"data\": \"table\", \"field\": \"Count\"},\n",
    "                \"range\": \"height\"\n",
    "            }\n",
    "        ],\n",
    "        \"axes\": [\n",
    "            { \"orient\": \"bottom\", \"scale\": \"xscale\" },\n",
    "            { \"orient\": \"left\", \"scale\": \"yscale\" }\n",
    "        ],\n",
    "        \"marks\": [\n",
    "            {\n",
    "                \"type\": \"rect\",\n",
    "                \"from\": {\"data\":\"table\"},\n",
    "                \"encode\": {\n",
    "                    \"enter\": {\n",
    "                        \"x\": {\"scale\": \"xscale\", \"field\": \"GeneID\"},\n",
    "                        \"width\": {\"scale\": \"xscale\", \"band\": 1},\n",
    "                        \"y\": {\"scale\": \"yscale\", \"field\": \"Count\"},\n",
    "                        \"y2\": {\"scale\": \"yscale\", \"value\": 0}\n",
    "                    },\n",
    "                    \"update\": {\"fill\": {\"value\": \"steelblue\"}},\n",
    "                    \"hover\": {\"fill\": {\"value\": \"red\"}}\n",
    "                }\n",
    "            },\n",
    "            {\n",
    "                \"type\": \"text\",\n",
    "                \"encode\": {\n",
    "                    \"enter\": {\n",
    "                        \"align\": {\"value\": \"center\"},\n",
    "                        \"baseline\": {\"value\": \"bottom\"},\n",
    "                        \"fill\": {\"value\": \"#333\"}\n",
    "                    },\n",
    "                    \"update\": {\n",
    "                        \"x\": {\"scale\": \"xscale\", \"signal\": \"tooltip.GeneID\", \"band\": 0.5},\n",
    "                        \"y\": {\"scale\": \"yscale\", \"signal\": \"tooltip.Count\", \"offset\": -2},\n",
    "                        \"text\": {\"signal\": \"tooltip.Count\"},\n",
    "                        \"fillOpacity\": [\n",
    "                            {\"test\": \"datum === tooltip\", \"value\": 0},\n",
    "                            {\"value\": 1}\n",
    "                        ]\n",
    "                    }\n",
    "                }\n",
    "            }\n",
    "        ]\n",
    "    }\n",
    "    )"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### 绘制疾病分布图"
   ],
   "execution_count": null,
   "outputs": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "def get_disease():\n",
    "    data = pd.read_csv(\"../Diseases.csv\")\n",
    "    data = data[\"Diseases\"]\n",
    "    data = list(data)\n",
    "    datalist = []\n",
    "    for i in data:\n",
    "        i = i.split('>',1)[1]\n",
    "        datalist.append(i)\n",
    "    datalist = np.unique(datalist, return_counts=True)\n",
    "    data = []\n",
    "    for i in range(len(datalist[0])):\n",
    "        dictionary = {}\n",
    "        if datalist[0][i]!='No Data':\n",
    "            dictionary['MeshID'] = datalist[0][i]\n",
    "            dictionary['Count'] = datalist[1][i]\n",
    "            data.append(dictionary)\n",
    "        \n",
    "    # 由于数值太多，因此对字典排序，选取最高的前10个数据\n",
    "    def takefifteen(x):\n",
    "        return x['Count']\n",
    "    data.sort(key=takefifteen,reverse=1)\n",
    "    data = data[0:10]\n",
    "    \n",
    "    Vega({\n",
    "        \"$schema\": \"https://vega.github.io/schema/vega/v5.json\",\n",
    "        \"width\": 1000,\n",
    "        \"height\": 500,\n",
    "        \"padding\": 5,\n",
    "        \"data\": [\n",
    "            {\n",
    "                \"name\": \"table\",\n",
    "                \"values\":data\n",
    "            }\n",
    "        ],\n",
    "        \"signals\": [\n",
    "            {\n",
    "                \"name\": \"tooltip\",\n",
    "                \"value\": {},\n",
    "                \"on\": [\n",
    "                    {\"events\": \"rect:mouseover\", \"update\": \"datum\"},\n",
    "                    {\"events\": \"rect:mouseout\",  \"update\": \"{}\"}\n",
    "                ]\n",
    "            }\n",
    "        ],\n",
    "        \"scales\": [\n",
    "            {\n",
    "                \"name\": \"xscale\",\n",
    "                \"type\": \"band\",\n",
    "                \"domain\": {\"data\": \"table\", \"field\": \"MeshID\"},\n",
    "                \"range\": \"width\",\n",
    "                \"padding\": 0.1\n",
    "            },\n",
    "            {\n",
    "                \"name\": \"yscale\",\n",
    "                \"domain\": {\"data\": \"table\", \"field\": \"Count\"},\n",
    "                \"range\": \"height\"\n",
    "            }\n",
    "        ],\n",
    "        \"axes\": [\n",
    "            { \"orient\": \"bottom\", \"scale\": \"xscale\" },\n",
    "            { \"orient\": \"left\", \"scale\": \"yscale\" }\n",
    "        ],\n",
    "        \"marks\": [\n",
    "            {\n",
    "                \"type\": \"rect\",\n",
    "                \"from\": {\"data\":\"table\"},\n",
    "                \"encode\": {\n",
    "                    \"enter\": {\n",
    "                        \"x\": {\"scale\": \"xscale\", \"field\": \"MeshID\"},\n",
    "                        \"width\": {\"scale\": \"xscale\", \"band\": 1},\n",
    "                        \"y\": {\"scale\": \"yscale\", \"field\": \"Count\"},\n",
    "                        \"y2\": {\"scale\": \"yscale\", \"value\": 0}\n",
    "                    },\n",
    "                    \"update\": {\"fill\": {\"value\": \"steelblue\"}},\n",
    "                    \"hover\": {\"fill\": {\"value\": \"red\"}}\n",
    "                }\n",
    "            },\n",
    "            {\n",
    "                \"type\": \"text\",\n",
    "                \"encode\": {\n",
    "                    \"enter\": {\n",
    "                        \"align\": {\"value\": \"center\"},\n",
    "                        \"baseline\": {\"value\": \"bottom\"},\n",
    "                        \"fill\": {\"value\": \"#333\"}\n",
    "                    },\n",
    "                    \"update\": {\n",
    "                        \"x\": {\"scale\": \"xscale\", \"signal\": \"tooltip.MeshID\", \"band\": 0.5},\n",
    "                        \"y\": {\"scale\": \"yscale\", \"signal\": \"tooltip.Count\", \"offset\": -2},\n",
    "                        \"text\": {\"signal\": \"tooltip.Count\"},\n",
    "                        \"fillOpacity\": [\n",
    "                            {\"test\": \"datum === tooltip\", \"value\": 0},\n",
    "                            {\"value\": 1}\n",
    "                        ]\n",
    "                    }\n",
    "                }\n",
    "            }\n",
    "        ]\n",
    "    }\n",
    "    )"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "accordion.children[2].on_click(on_button_click_wordcloud)\n",
    "accordion.children[3].on_click(on_button_click_gene)\n",
    "accordion.children[4].on_click(on_button_click_disease)\n",
    "display(accordion, output)"
   ]
  }
 ],
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        "1": "请输入检索文献的数量",
        "2": "绘制词云图",
        "3": "绘制基因分布图",
        "4": "绘制疾病分布图"
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      "model_module_version": "1.5.0",
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              "BACKGROUND/AIM: Chemotherapy with docetaxel (DTX) is used for castration-resistant prostate cancer (CRPC), but it is inadequate. MATERIALS AND METHODS: We evaluated the effect of the combination treatment DTX and the mTOR inhibitor temsirolimus (TEM) in the PC3 prostate cancer cell line, by focusing on the induction of autophagy and apoptosis. RESULTS: TEM induced autophagy but not apoptosis even at a high dose, whereas DTX induced apoptosis. The combination of low-dose DTX and TEM caused a 34% suppression in cell proliferation compared to monotherapy with a higher dose of DTX. The induction of apoptosis was increased by their combination. The combination with DTX overcame the induction of autophagy by TEM. The combination treatment suppressed tumor growth 72% less than the control group after 14 days of treatment in vivo. CONCLUSION: The combination of TEM and DTX induced apoptosis by overcoming autophagy and enhanced the anticancer effect compared to monotherapy.",
              "BACKGROUND/AIM: Tubugi-1 is a more stable and accessible synthetic counterpart of natural tubulysins. This study aimed to evaluate its cytotoxic potential against anaplastic human melanoma cells. MATERIALS AND METHODS: The viability of A-375 cells was determined by 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assay. The type of cell death and proliferative rate were investigated using flow cytometry and fluorescent microscopy, while the molecular background was evaluated by western blot. RESULTS: Tubugi-1 reduced the viability of A-375 cells, inducing massive micronucleation, followed by augmented expression of inhibitor of nuclear factor-kappaB and caspase-2, typical of a mitotic catastrophe. Disturbed proliferation and G2M block with prominent caspase activity, weakened the expression of B-cell lymphoma 2 and B-cell lymphoma 2-associated X transient up-regulation, coexisted with intensive autophagy. Specific inhibition of autophagy by chloroquine resulted in conversion from mitotic catastrophe to rapid apoptosis. CONCLUSION: Multilevel anticancer action of tubugi-1 is extended by co-application of an autophagy inhibitor, giving a new dimension in further preclinical advancement of this potential agent.",
              "Objective To investigate the effects of Astragalus polysaccharide (APS) on autophagy and expression of microtubule-associated protein 1 light chain 3B (LC3B), mammalian target of rapamycin (mTOR) and beclin1 in xanthine oxidase (XOD)-induced autophagic model of non-small cell lung cancer A549 cells. Methods A549 cells were divided into five groups: control group, model group, 100, 200 and 400 mug/mL APS-treated group. Except for control group, all groups were administered XOD for 24 hours to establish autophagic models. Morphology of autophagosome was detected by transmission electron microscopy (TEM) and the number was counted by monodansylcadaverine (MDC) staining. The expression levels of LC3B, beclin1 and mTOR were detected by Western blot analysis. Results Compared with the control group, the number of autophagosome in the model group increased; the expression of LC3B and beclin1 significantly increased; while the expression of mTOR significantly decreased. Compared with the model group, the number of autophagosome decreased remarkably; the expression of LC3B and beclin1 severely decreased, and the expression of mTOR obviously increased in 200 or 400 mug/mL APS-treated group. Conclusion APS reduces the level of autophagy, down-regulates the expression of LC3B and beclin1, and increases mTOR expression in the autophagic model of A549 cells induced by XOD.",
              "Objective To investigate the effect of baicalein (BAI) on autophagy in gastric cancer cell line MGC-803. Methods MGC-803 cells were treated with 0, 5, 15, 25, 50 mumol/L BAI for 24, 48, 72 hours. The proliferation activity of MGC-803 cells was detected by MTT assay. Acridine orange (AO) staining combined with immunofluorescence cytochemical staining was performed to observe the expression of microtubule-associated protein 1 light chain 3 (LC3) and P62 to determine autophagy in MGC-803 cells. The protein levels of LC3, P62, phosphatidylinositol 3-kinase (PI3K), phosphorylated PI3K (p-PI3K), AKT, and p-AKT were detected by Western blot analysis. Results Compared with the control group, BAI significantly inhibited the proliferation of MGC-803 cells in a time- and dose-dependent manner. BAI-treated MGC-803 cells showed a significant increase in acid lysosomes and increased LC3 expression. BAI treatment significantly decreased phosphorylation of PI3K and AKT proteins, increased the ratio of LC3-II/LC3-I and up-regulated the expression of P62 protein. Conclusion Baicalein could inhibit PI3K/AKT signaling pathway and induce autophagy in MGC-803 cells.",
              "The resurgence of Drosophila as a recognized model for carcinogenesis has contributed greatly to our conceptual advance and mechanistic understanding of tumor growth in vivo. With its powerful genetics, Drosophila has emerged as a prime model organism to study cell biology and physiological functions of autophagy. This has enabled exploration of the contributions of autophagy in several tumor models. Here we review the literature of autophagy related to tumorigenesis in Drosophila. Functional analysis of core autophagy components does not provide proof for a classical tumor suppression role for autophagy alone. Autophagy both serve to suppress or support tumor growth. These effects are context-specific, depending on cell type and oncogenic or tumor suppressive lesion. Future delineation of how autophagy impinges on tumorigenesis will demand to untangle in detail, the regulation and flux of autophagy in the respective tumor models. The downstream tumor-regulative roles of autophagy through organelle homeostasis, metabolism, selective autophagy or alternative mechanisms remain largely unexplored.",
              "Previous studies have shown that selenite, a representative of inorganic form selenium, exerts its anticancer effect by inducing apoptosis in androgen-dependent LNCaP prostate cancer cells, but few studies have determined the nature of cell death induced by selenite in metastatic androgen-refractory PC-3 cells. Our study showed that necrosis-like cell death rather than apoptosis, pyroptosis, or autophagic cell death was caused by selenite in PC-3 cells. Mechanistically, this type of cell death was caused by ATP depletion (26.28 +/- 3.39 nmol/mg of control versus 9.12 +/- 2.44 nmol/mg of 10 muM selenite treatment) that resulted from phosphofructokinase activity reduction (100.17 +/- 0.17% of control versus 21.74 +/- 6.65% of 10 muM selenite treatment). Our study also showed that ROS production is necessary for the decrease in cellular ATP levels and in phosphofructokinase activity. To our knowledge, this is the first study showing that selenite can induce necrosis-like cell death in PC-3 cells. Our findings support selenite as an effective compound for the therapy of apoptosis-resistant prostate cancer.",
              "BACKGROUND: Autophagy plays an important role in regulating cisplatin (CDDP) resistance in gastric cancer cells. However, the underlying mechanism of methioninase (METase) in the regulation of autophagy and CDDP resistance of gastric cancer cells is still not clear. MATERIALS AND METHODS: Western blot was used to detect the levels of autophagy-related proteins, multidrug-resistant 1 (MDR-1), and FoxM1 protein. LncRNA HULC was detected by qRT-PCR. Cell viability was detected using CCK-8 assay. The interaction between lncRNA HULC and FoxM1 was confirmed by RNA pull-down and RIP assay. RESULTS: Lentiviral vector carrying METase (LV-METase) suppressed autophagy and CDDP resistance of drug-resistant gastric cancer cells. LncRNA HULC was significantly downregulated in drug-resistant gastric cancer cells transfected with LV-METase. Besides, we found that lncRNA HULC interacted with FoxM1. In addition, METase suppressed autophagy to reduce CDDP resistance of drug-resistant gastric cancer cells through regulating HULC/FoxM1, and interfering HULC suppressed autophagy to reduce CDDP resistance of drug-resistant gastric cancer cells through regulating FoxM1. Finally, interfering HULC inhibited tumor growth in vivo. CONCLUSION: METase suppressed autophagy to reduce CDDP resistance of drug-resistant gastric cancer cells through regulating HULC/FoxM1 pathway.",
              "The term \"autophagy\", which means \"self (auto) - eating (phagy)\", describes a catabolic process that is evolutionarially conserved among all eukaryotes. Although autophagy is mainly accepted as a cell survival mechanism, it also modulates the process known as \"type II cell death\". AKT/mTOR pathway is an upstream activator of autophagy and it is tightly regulated by the ATG (autophagy-related genes) signaling cascade. In addition, wide ranging cell signaling pathways and non-coding RNAs played essential roles in the control of autophagy. Autophagy is closely related to pathological processes such as neurodegenerative diseases and cancer as well as physiological conditions. After the Nobel Prize in Physiology or Medicine 2016 was awarded to Yoshinori Ohsumi \"for his discoveries of mechanisms for autophagy\", there was an explosion in the field of autophagy and molecular biologists started to pay considerable attention to the mechanistic insights related to autophagy in different diseases. Since autophagy behaved dualistically, both as a cell death and a cell survival mechanism, it opened new horizons for a deeper analysis of cell type and context dependent behavior of autophagy in different types of cancers. There are numerous studies showing that the induction of autophagy mechanism will promote survival of cancer cells. Since autophagy is mainly a mechanism to keep the cells alive, it may protect breast cancer cells against stress conditions such as starvation and hypoxia. For these reasons, autophagy was noted to be instrumental in metastasis and drug resistance. In this chapter we have emphasized on role of role of autophagy in breast cancer. Additionally we have partitioned this chapter into exciting role of microRNAs in modulation of autophagy in breast cancer. We have also comprehensively summarized how TRAIL-mediated signaling and autophagy operated in breast cancer cells.",
              "Mitochondrial dynamics, involving mitochondrial fusion, fission and autophagy, plays an important role in maintaining cellular physiological function and homeostasis. Mitochondria are the \"energy plant\" of human body, so the changes of mitochondrial fusion, division and autophagy are important for cell respiration and energy production. On the other hand, energy metabolism influences mitochondrial dynamics in turn. This paper reviewed the recent advances in studies on the relationship between energy metabolism and the proteins regulating mitochondrial fusion, fission and autophagy. The association of mitochondrial dynamics with electron chain complex expression, oxidative phosphorylation and ATP synthesis upon exercise intervention will provide theoretical references for the further studies in sports training and disease intervention.",
              "PURPOSE: Long intergenic non-protein coding RNA 665 (LINC00665) plays a vital role in the development of cancer. Its function in hepatocellular carcinoma (HCC), however, remains largely unknown. MATERIALS AND METHODS: The expressions of LINC00665, miR-186-5p, and MAP4K3 were determined by qRT-PCR. Cell viability and apoptosis were evaluated by MTT and flow cytometry, respectively. Autophagic puncta formation was observed by fluorescence microscopy. Bioinformatics analysis, luciferase reporter assay, RNA immunoprecipitation, and RNA pulldown were performed to identify associations among LINC00665, miR-186-5p, and MAP4K3. Western blot was utilized to examine the expressions of MAP4K3, Beclin-1, and LC3. Tumor growth was evaluated in a xenograft model. RESULTS: Elevations in LINC00665 were observed in HCC tissues and cells. The overall survival of HCC patients with high levels of LINC00665 was shorter than those with low levels. In vitro, LINC00665 depletion inhibited viability and induced apoptosis and autophagy. miR-186-5p interacted with LINC00665 and was downregulated in HCC tissues and cells. Upregulation of miR-186-5p inhibited viability and induced apoptosis and autophagy, which were attenuated by upregulation of LINC00665. MAP4K3 was found to possess binding sites with miR-186-5p and was upregulated in HCC tissues and cells. MAP4K3 depletion inhibited viability and induced apoptosis and autophagy, which were attenuated by miR-186-5p inhibitor. In vivo, miR-186-5p expression was negatively correlated with LINC00665 or MAP4K3 in HCC tissues, while LINC00665 was positively correlated with MAP4K3. LINC00665 knockdown suppressed tumor growth. CONCLUSION: LINC00665 was involved in cell viability, apoptosis, and autophagy in HCC via miR-186-5p/MAP4K3 axis, which may provide a new approach for HCC treatment.",
              "Polyphyllin I (PPI), a bioactive component extracted from Paris polyphylla, was reported to have potent anticancer activities in previous studies. However, there were few reports on the effects and underlying mechanism of PPI in human acute myeloid leukemia cells. The present study demonstrated that PPI had an inhibitory effect through inducing apoptosis and autophagy in THP-1 and NB4 cells. PPI induced apoptosis via activating JNK pathway, as evidenced by the decreased Bcl-2 levels and increased Bax, cleaved-caspase-3 and phosphorylated-JNK expressions. In addition, PPI promoted autophagy as evidenced with increased expressions of LC3-II and Beclin-1 in western blot and autophagic vacuoles in MDC staining, which was associated with the inhibition of AKT-mTOR pathway. Furthermore, JNK inhibitor SP600125 and autophagy inhibitor 3-MA were employed to evaluate the role of apoptosis and autophagy in PPI-induced cell death. We found that autophagy and apoptosis were both causes of cell death induced by PPI. These data suggested that PPI could be a potent therapeutic agent for the treatment of human acute myeloid leukemia.",
              "Citreoviridin (CIT), a mycotoxin and ATP synthase inhibitor, is regarded as one of aetiology factors of cardiac beriberi and Keshan disease. Thiamine (VB1) and selenium (Se) improve the recovery of these two diseases respectively. The underlying mechanisms of cardiotoxic effect of CIT and cardioprotective effect of VB1 and Se have not been fully elucidated. In this study, we found that ectopic ATP synthase was more sensitive to CIT treatment than mitochondrial ATP synthase in H9c2 cardiomyocytes. CIT inhibited the transcriptional activity of peroxisome proliferator activated receptor gamma (PPAR-gamma) in mice hearts and H9c2 cells. PPAR-gamma agonist attenuated the inhibitory effect of CIT on mechanistic target of rapamycin complex 2 (mTORC2) and stimulatory effect of CIT on autophagy in cardiomyocytes. CIT induced apoptosis through lysosomal-mitochondrial axis in cardiomyocytes. PPAR-gamma agonist and autophagy inhibitor alleviated CIT-induced apoptosis and accelerated cardiac biomarker. VB1 and Se accelerated the basal transcriptional activity of PPAR-gamma in mice hearts and H9c2 cells. Furthermore, VB1 and Se reversed the effect of CIT on PPAR-gamma, autophagy and apoptosis. Our findings defined PPAR-gamma-mTORC2-autophagy pathway as the key link between CIT cardiotoxicity and cardioprotective effect of VB1 and Se. The present study would shed new light on the pathogenesis of cardiomyopathy and the cardioprotective mechanism of micronutrients.",
              "OBJECTIVE: To investigate the effect of miR-30a/HMGA2-mediated autophagy in osteosarcoma cells on apoptosis induced by chemotherapeutics. Methods: A total of 30 osteosarcoma tissues of sensitive and resistant to chemotherapeutics were divided into a chemotherapy-sensitive group and a chemotherapy-resistant group. The mRNA expression levels of miR-30a and high mobility group protein A2 (HMGA2) in the chemotherapy-sensitive group and the chemotherapy-resistant group, and the mRNA expression levels of miR-30a in osteosarcoma U2-OS cells treated by cisplatin, doxorubicin and methotrexate at different concentrations were detected by real-time PCR. The expression levels of autophagy related protein Beclin 1, microtubule associated protein 1 light chain 3B (LC3B) and autophagy factor P62 were detected by Western blotting. The osteosarcoma U2-OS cells were transfected with miR-30a mimics and miR-30a inhibitors to construct a miR-30a high expression group, a miR-30a low expression group and a control group. The expression levels of Beclin 1, LC3B and P62 in osteosarcoma U2-OS cells after treatment of cisplatin and doxorubicin in these 3 groups were detected by Western blotting; the level of autophagy was detected by monodansylcada (MDC) staining; the level of ROS was detected by dihydroethidium (DHE); the level of cell surviving rate was detected by cell counting kit-8 (CCK-8); the level of apoptosis was detected by annexin APC/PI double staining; the level of mitochondria oxidative damage was detected by mitochondrial membrane potential assay kit with JC-1 (JC-1 method). The interaction between miR-30a and HMGA2 was detected by dual luciferase reporter assay. The osteosarcoma U2-OS cells were transfected with HMGA2 mimics and HMGA2-shRNA to construct a high HMGA2 group, a low HMGA2 group, and a control group. The expression levels of Beclin 1, LC3B and P62 in osteosarcoma U2-OS cells after the treatment of cisplatin were detected by Western blotting. Results: The level of miR-30a in the chemotherapy-resistant tissues was significantly lower than that in the chemotherapy-sensitive tissues (P<0.05), and the expression of HMGA2 was opposite comparing to that of miR-30a (P<0.05). After the treatment by low concentration (5 mumol/L) of chemotherapeutics, the level of miR-30a was down-regulated in osteosarcoma U2-OS cells, accompanied with up-regulation of Beclin 1 and LC3B (P<0.01) and down-regulation of P62 (P<0.01). Compared with the control group, the expression levels of Beclin 1 and LC3B were significantly decreased (P<0.05), and the expression level of P62 was significantly increased (P<0.05) in the miR-30a high expression group, which was opposite in the miR-30a low expression group. In the miR-30a high expression group treated by chemotherapeutics, the level of autophagy and the cell survival rate were lower than those in group with low expression of miR-30a, while the levels of ROS, the mitochondrial oxidative damage and the apoptosis were higher than those in group with low expression of miR-30a (all P<0.05). The targeting interaction between HMGA2 and miR-30a were verified by dual luciferase reporter assay. Compared with the control group, the expression levels of Beclin 1 and LC3B were significantly increased (P<0.05), and the expression level of P62 was significantly decreased (P<0.05) in the HMGA2 high expression group, which was opposite in the HMGA2 low expression group. Conclusion: Suppression of miR-30a/HMGA2-mediated autophagy in osteosarcoma cells is likely to enhance the therapeutic effect of chemotherapeutics.",
              "In this work, a unique dual-site controlled fluorescent probe was presented for the sensitive and concurrent detection of pH in the cytoplasm and lysosomes. With the probe, the simultaneous down-regulation of pH in the lysosomes and cytoplasm during autophagy has been successfully revealed for the first time.",
              "This work aimed to demonstrate a new strategy for enhancing the production of carotenoids through the regulation of seesaw cross-talk between autophagy and carotenoid biosynthesis pathways in Chlamydomonas reinhardtii. Autophagy-related ATG1 and ATG8 genes were first silenced using artificial microRNA, which in turn reduced the mRNA expression of ATG1 and ATG8 by 84.4% and 74.3%, respectively. While ATG1 kinase controls early step in autophagy induction and ATG8 is an essential factor for the downstream formation of autophagosome membranes, the decreased expression of these genes led to a 2.34-fold increase in the amount of beta-carotene content (i.e., 23.75mg/g DCW). Furthermore, all mutants seemed to exhibit greater biodiesel properties than that of wild-type due to increased accumulation of saturated and monounsaturated fatty acids. These results support the role of autophagy in regulating the production of valuable metabolites, which could contribute to uplifting the economic outlook of nascent algal biorefinery.",
              "The cytotoxicity of a dinuclear imine-copper (II) complex 2, and its analogous mononuclear complex 1, toward different melanoma cells, particularly human SKMEL-05 and SKMEL-147, was investigated. Complex 2, a tyrosinase mimic, showed much higher activity in comparison to complex 1, and its reactivity was verified to be remarkably activated by UVB-light, while the mononuclear compound showed a small or negligible effect. Further, a significant dependence on the melanin content in the tumor cells, both from intrinsic pigmentation or stimulated by irradiation, was observed in the case of complex 2. Similar tests with keratinocytes and melanocytes indicated a much lower sensitivity to both copper (II) complexes, even after exposition to UV light. Clonogenic assays attested that the fractions of melanoma cells survival were much lower under treatment with complex 2 compared to complex 1, both with or without previous irradiation of the cells. The process also involves generation of reactive oxygen species (ROS), as verified by EPR spectroscopy, and by using fluorescence indicators. Autophagic assays indicated a remarkable formation of cytoplasmic vacuoles in melanomas treated with complex 2, while this effect was not observed in similar treatment with complex 1. Monitoring of specific protein LC3 corroborated the simultaneous occurrence of autophagy. A balance interplay between different modes of cell death, apoptosis and autophagy, occurs when melanomas were treated with the dinuclear complex 2, in contrast to the mononuclear complex 1. These results pointed out to different mechanisms of action of such complexes, depending on its nuclearity.",
              "Renal fibrosis is the final common pathway of all chronic kidney diseases progressing to end-stage renal diseases. Autophagy, a highly conserved lysosomal degradation pathway, plays important roles in maintaining cellular homeostasis in all major types of kidney cells including renal tubular cells as well as podocytes, mesangial cells and endothelial cells in glomeruli. Autophagy dysfunction is implicated in the pathogenesis of various renal pathologies. Here, we analyze the pathological role and regulation of autophagy in renal fibrosis and related kidney diseases in both glomeruli and tubulointerstitial compartments. Further research is expected to gain significant mechanistic insights and discover pathway-specific and kidney-selective therapies targeting autophagy to prevent renal fibrosis and related kidney diseases.",
              "The present study aims to evaluate the effect of the ethyl acetate extract of Cichorium (EAEC) as a novel photosensitizer in photodynamic therapy (PDT) of colorectal carcinoma (CRC) HCT116 and SW620 cells. The absorption and fluorescence spectra of EAEC were measured using a UV-vis spectrophotometer and fluorescence spectrophotometer, respectively. EAEC-induced reactive oxygen species (ROS) production in HCT116 and SW620 cells was detected using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and glutathione/glutathione disulfide (GSH/GSSG). The photo- and dark toxicities of EAEC were estimated using the Cell Counting Kit-8 (CCK-8) assay. Cellular uptake and localization of EAEC were detected by confocal laser fluorescence microscopy. Annexin V-FITC/PI staining, Western blotting and immunofluorescence staining were used to assess apoptosis and autophagy. The antitumor activity of EAEC was confirmed in a xenograft model. Finally, effects on the PERK pathway were verified using qRT-PCR and Western blotting. EAEC displayed absorption and fluorescence emission peaks at 660 nm and 678 nm, respectively. EAEC induced ROS production in CRC cells. Assessment of dark toxicity showed that treatment with EAEC alone induced little cytotoxicity in CRC or normal cells but that EAEC-PDT induced significant photocytotoxicity in CRC cells in a time- and dose-dependent manner. After cellular uptake, EAEC was located in the mitochondria. Treatment with EAEC-PDT reduced xenograft tumor size. Further evaluation suggested that activation of the PERK pathway mediates these effects, as the apoptotic rate and autophagy flux increased markedly after EAEC-PDT. EAEC, a natural photosensitizer extracted from Cichorium, displays potential utility in PDT of CRC by targeting the PERK pathway.",
              "Peritoneal dissemination is the most frequent metastatic route of ovarian cancer. However, due to the high heterogeneity in ovarian cancer, most conventional studies lack parental tumor controls relevant to metastases and, thus, it is difficult to trace the molecular changes of cancer cells along with the selection by the abdominal microenvironment. Here, we established an in vivo mouse peritoneal dissemination scheme that allowed us to select more aggressive sublines from parental ovarian cancer cells, including A2780 and SKOV-3. Microarray and gene profiling analyses indicated that autophagy-related genes were enriched in selected malignant sublines. Detection of LC3-II, p62 and autophagic puncta demonstrated that these malignant variants were more sensitive to autophagic induction when exposed to diverse stress conditions, such as high cell density, starvation and drug treatment. As compared with parental A2780, the selected variant acquired the ability to grow better under high-density stress; however, this effect was reversed by addition of autophagic inhibitors or knockdown of ATG5. When analyzing the clinical profiles of autophagy-related genes identified to be enriched in malignant A2780 variant, 73% of them had prognostic significance for the survival of ovarian cancer patients. Taken together, our findings indicate that an increase in autophagic potency among ovarian cancer cells is crucial for selection of metastatic colonies in the abdominal microenvironment. In addition, the derived autophagic gene profile can not only predict prognosis well but can also be potentially applied to precision medicine for identifying those ovarian cancer patients suitable for taking anti-autophagy cancer drugs.",
              "OBJECTIVES: To explore the effects of carbon disulfide (CS2) and N-carbamoyl glutamate (NCG) on autophagy during the window of embryo implantation in mice and whether dietary NCG supplementation can promote embryo implantation in case of CS2 exposure. METHODS: Pregnant mice that received single intraperitoneal injection of CS2 on Gestational day (GD)4 were fed basal diet with or without NCG supplementation from GD1 to endpoints. The control mice were injected solvents. There were four endpoints (GD5, GD6, GD7 and GD9 endpoints) in each group. The uterus was collected on endpoints to detect autophagy-related markers by using the methods of transmission electron microscopy (TEM), immunohistochemistry (IHC), quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA. RESULTS: The P62 brown punctate staining increased in CS2 exposure group and reduced after dietary NCG supplementation, which was opposite with LC3B, Beclin1 and ATG5 on GD5 endpoint. Simultaneously, P62 protein expression raised 43.33% on GD5 endpoint (p<0.01) when exposed to CS2 and descended to the control level after NCG supplementation. The rate of decline of LC3B and Beclin1 proteins were 27.04% (p<0.01) and 23.27% (p<0.05) on GD5 endpoint, 20.20% (p<0.05) and 11.30% on GD7 endpoint in CS2 exposure group, respectively, then NCG supplementation caused the LC3B and Beclin1 protein expression to rise in different degrees. Comparatively, the mRNA expression of all autophagy-related gene changed more apparently on three endpoints than the protein expression. The images of TEM showed that nearly no autophagosome could be seen in CS2 exposure group, while dietary NCG supplementation increased the number of autophagosome obviously on GD5 endpoint. The number of implanted embryos which declined due to CS2 exposure returned to normal in NCG supplementation group. CONCLUSIONS: Dietary NCG supplementation could rescue the suppressed autophagy induced by CS2 in the window of implantation and increase the number of implanted embryos.",
              "OBJECTIVE: To explore the effect of electroacupuncture (EA) at \"Zusanli\" (ST36) on gastrointestinal motility and expression of autophagy marker LC3 and autophagy signaling pathway molecule AMP-activated protein kinase (AMPK) in rats with functional dyspepsia (FD), so as to explore its mechanisms underlying improvement of FD. METHODS: A total of 40 male SD rats were randomly divided into blank control, model, EA, AMPK inhibitor and EAAMPK inhibitor groups, with 8 rats in each group. The FD model was established by tail-clip (30 min/time, twice daily) single day feeding, and gavage of normal saline (2 mL/time, twice a day) for 2 successive weeks. For rats of EA and EAAMPK inhibitor groups, EA (4 Hz, 1.0 mA) was applied to bilateral ST36 for 20 min, once daily for 7 successive days. For rats of the AMPK-inhibitor and EAAMPK inhibitor groups, Compound C (20 mg/kg) solution was administered by intraperitoneal injection before every EA administration. The gastric residual rate and small intestinal transit rate were calculated based on the weight of stomach and length of ink propelling and total small intestine, respectively. The expression levels of c-kit, microtubule-associated protein 1 light chain 3, Beclin 1, phosphorylated (p)-AMPK and p-unc-51 like autophagy activating kinase 1(ULK1) in the gastric antrum tissue were detected by using Western blot. RESULTS: Compared with the blank control group, the gastric residual rate and the expression levels of LC3-/LC3 Beclin 1, p-AMPK and p-ULK1 proteins were significantly increased, and the small intestinal transit rate and the expression of c-kit protein obviously decreased in the model group (P<0.01). After EA intervention, modeling-induced increase of gastric residual rate and the expression of LC3-/LC3 Beclin 1, p-AMPK and p-ULK1 proteins, and decrease of small intestinal transit rate and expression of c-kit protein were reversed in the EA, AMPK inhibitor and EAAMPK inhibitor groups (P<0.05, P<0.01). The therapeutic effect of EA and EAAMPK was significantly superior to that of AMPK inhibitor in down-regulating the expression of LC3/LC3 Beclin 1, p-AMPK and p-ULK1 proteins and in up-regulating the expression of c-kit protein (P<0.05, P<0.01). No significant differences were found among the EA, AMPK inhibitor and EAAMPK inhibitor groups in lowering gastric residual rate and elevating the small intestinal transit rate (P>0.05). CONCLUSION: EA at ST36 can promote gastrointestinal motility in FD rats, which is possibly mediated by inhibiting excessive autophagy of interstitial cells of Cajal via down-regulating AMPK/ULK1 signaling.",
              "Excessive autophagy is one of the crucial factors of cerebral ischemia-reperfusion injury (CIRI), which has been demonstrated to be one of the targets for acupuncture treatment of ischemic stroke. In the present paper, we make a review about the development of acupuncture intervention induced improvement of CIRI (such as reducing the infarction area, improving learning-memory ability and motor function) by regulating autophagy in animal studies. Outcomes showed that acupuncture intervention can function in 1) inhibiting CIRI-induced increase of the number of lysosomes and autophagic lysosomes, and relieving structural injury of mitochondria, and reducing the number of autophagosome in the central region of the ischemic cerebral cortex tissue; 2) down-regulating the expression of microtubule-associated protein light chain 3 (LC3) and the ratio of LC3-/LC3- in the ischemic cerebral region, and 3) regulating the expression of Beclin 1 (autophagy-related gene), promoting the expression of P62 (autophagy-related adaptor protein). In addition, acupuncture can also regulate phosphoinositide 3 kinase (PI3K)- protein kinase B (AKT)- mammalian target of rapamycin complex 1(mTOR) signaling at different time-points (down-regulation at the early stage and up-regulation at the later stage), and activating AMP-activated protein kinase (AMPK)-mTOR- UNC51-like kinase-1 signaling to relieve cerebral ischemic injury. These results reveal some mechanisms of acupuncture therapy underlying improvement of CIRI and provide experimental basis for clinical application of acupuncture therapy in the treatment of ischemic stroke.",
              "Sepsis, defined as life-threatening tissue damage and organ dysfunction caused by a dysregulated host response to infection, is a critical disease which imposes global health burden. Sepsis-induced organ dysfunction, including circulatory and cardiac dysfunction, hepatic dysfunction, renal dysfunction, etc., contributes to high mortality and long-term disability of sepsis patients. Altered inflammatory response, ROS and reactive aldehyde stress, mitochondrial dysfunction, and programmed cell death pathways (necrosis, apoptosis, and autophagy) have been demonstrated to play crucial roles in septic organ dysfunction. Unfortunately, except for infection control and supportive therapies, no specific therapy exists for sepsis. New specific therapeutic targets are highly warranted. Emerging studies suggested a role of potential therapeutic target of ALDH2, a tetrameric enzyme located in mitochondria to detoxify aldehydes, in septic organ dysfunction. In this article, we will review the presentations and pathophysiology of septic organ dysfunction, as well as summarize and discuss the recent insights regarding ALDH2 in sepsis.",
              "Leishmaniasis comprises a group of infectious diseases with worldwide distribution, of which both the visceral and cutaneous forms are caused by Leishmania parasites. In the absence of vaccines, efficacious chemotherapy remains the basis for leishmaniasis control. The available drugs are expensive and associated with several secondary adverse effects. Due to these limitations, the development of new antileishmanial compounds is imperative, and plants offer various perspectives in this regard. The present study evaluated the in vitro leishmanicidal activity of flavonoids isolated from Solanum paludosum Moric. and investigated the mechanisms of cell death induced by them. These compounds were evaluated in vitro for their antileishmanial activity against Leishmania amazonensis promastigotes and they showed prominent leishmanicidal activity. The EtOAc fraction, gossypetin 3,7,8,4'-tetra-O-methyl ether (1), and kaempferol 3,7-di-O-methyl ether (3) were selected to be used in an in vitro assay against L. amazonensis amastigotes and cell death assays. The flavonoids (1) and (3) presented significant activity against L. amazonensis amastigotes, exhibiting the IC50 values of 23.3 +/- 4.5 muM, 34.0 +/- 9.6 muM, and 10.5 +/- 2.5 muM for the EtOAc fraction, (1), and (3), respectively, without toxic effects to the host cells. Moreover, (1) and (3) induced blocked cell cycle progression at the G1/S transition, ultimately leading to G1/G0 arrest. Flavonoid (3) also induced autophagy. Using Raman spectroscopy in conjunction with principal component analysis, the biochemical changes in the cellular components induced by flavonoids (1) and (3) were presented. The obtained results indicated that the mechanisms of action of (1) and (3) occurred through different routes. The results support that the flavonoids derived from S. paludosum can become lead molecules for the design of antileishmanial prototypes.",
              "AIMS: To explore the mechanism of how LSD1 regulates autophagy and the correlation between LSD1 and Ox-LDL-induced inflammation. MAIN METHODS: RAW264.7 cells were used during the whole study. Firstly, the effect of Ox-LDL-stimulation on LSD1 expression was detected. Through loss-of-function assay, the associations between LSD1 interference and SESN2 expression, autophagy, NLRP3 inflammasome and inflammatory cytokines were explored. Finally, the function of LSD1 exerted on activation of PI3K/Akt/mTOR signal pathway was detected using western blotting assay. KEY FINDINGS: The expression of LSD1 was significantly elevated in Ox-LDL-treated RAW264.7 cells. Inhibition of LSD1 promoted autophagy, inhibited inflammation and activated NLRP3 inflammasome. SESN2 was elevated by LSD1 inhibition, and thus activate the PI3K/Akt/mTOR signal pathway. What' more, Knockdown of SESN2 or deactivate the PI3K/Akt/mTOR signal pathway partly reversed the effect of LSD1 inhibition on autophagy. SIGNIFICANCE: Our present study drew the finding that the knockdown of LSD1 meliorated Ox-LDL-stimulated NLRP3 activation and inflammation through promoting autophagy via SESN2-mediated PI3K/Akt/mTOR pathway.",
              "Neurotoxicity caused by particulate matter (PM) has been highlighted as being a potential risk factor for neurodegenerative diseases. However, the effects of brain inflammation in response to traffic-related PM remain unclear. The objective of this study was to investigate the effects of traffic-related PM on microglial responses. We determined the cytotoxicity, oxidative stress, lipid peroxidation, inflammation, activation, autophagy, and apoptosis due to exposure to carbon black (CB) and diesel exhaust particles (DEPs) in Bv2 microglial cells. Additionally, cells were pretreated with corticosteroid to determine alterations in microglial activation and inflammation. For in vivo confirmation, Sprague Dawley (SD) rats were whole-body exposed to traffic-related PM1 (PM with an aerodynamic diameter of <1mum) for 3 and 6 months. We observed that a decrease in cell viability and increases in dichlorodihydrofluorescein (DCFH), lactate dehydrogenase (LDH), and thiobarbituric acid-reactive substances (TBARSs) occurred due to CB and DEP. Production of interleukin (IL)-6 and soluble tumor necrosis factor (TNF)-alpha was significantly stimulated by CB and DEP, whereas production of cellular TNF-alpha was significantly stimulated by CB. Iba1 and prostaglandin E2 (PGE2) significantly increased due to CB and DEP. Consistently, we observed significant increases in Iba1 in the hippocampus of rats after 3 and 6 months of exposure to traffic-related PM1. We found that the light chain 3II (LC3II)/LC3I ratio and caspase-3 activity increased due to CB and DEP exposure. Subsequently, LDH, TBARS, LC3II/I, and caspase-3 activities did not clearly respond to corticosteroid pretreatment followed by DEP exposure in BV2 cells. Results of the present study suggested that traffic-related PM induced cytotoxicity, lipid peroxidation, microglial activation, and inflammation as well as autophagy and caspase-3 regulation in microglia. We demonstrated that microglial activation and inflammation may play important roles in the response of the brain to traffic-related PM.",
              "Ischemia-reperfusion injury is a major reason for acute kidney injury and various kidney diseases. Autophagy plays an important role during renal ischemia-reperfusion injury (RIRI), but it remains controversial whether autophagy contributes to cell survival or ischemia-reperfusion-induced cell death. In the review, we summarized the function of autophagy in the progression of acute ischemic kidney injury, as well as its related molecular mechanisms. While analyzing the opposite roles of autophagy in RIRI, it was concluded that the protective or detrimental function of autophagy was depending on the timing and amount of the activation of cell autophagy. We also summarized the regulatory agents, including active compounds, proteins, or microRNAs (miRNAs), which regulated the cell autophagy during renal acute ischemic kidney injury process. This explained why the opposite conclusion occurred when cell autophagy was studied in the RIRI models from different researchers. Therefore, the article provided a hypothesis to control cell autophagy at the appropriate timing and intensity so as to alleviate renal injury and sustain cell survival of the renal cell.",
              "A novel dual-mode nanoprobe (Apt@MNPS) was created for the detection of autophagy-related miRNAs to monitor the autophagic level and study the effect of PTT on autophagy. Interestingly, using our developed probe, PTT was found to be able to activate the autophagy by down regulation of miR-18a* and miR-4802, which in turn restricted the PTT efficiency for cancer.",
              "Recent studies have discovered a selective autophagy-lipophagy, which can selectively identify and degrade lipids and plays an important role in regulating cellular lipid metabolism and maintaining intracellular lipid homeostasis. The process of lipophagy can be directly or indirectly regulated by genes, enzymes, transcriptional regulators and other factors. This review examines the role of lipophagy in reducing liver lipid content, regulating pancreatic lipid metabolism, and regulating adipose tissue differentiation, and summarizes the findings of the molecules (Rab GTPase, enzymes, ion channels, transcription factors, small molecular substances) involved in the regulation of lipophagy, which points to new directions for the treatment of diseases caused by lipid accumulation.",
              "OBJECTIVE: To investigate the inhibitory effect of matrine on the proliferation of human non-small cell lung cancer (NSCLC) and explore the possible molecular mechanism. METHODS: Cultured human NSCLC A549 cells were treated with 0.4, 0.8, 1.2, 1.6, and 2.0 g/L matrine for 24, 48 or 72 h. CCK-8 assay was used for measuring the changes in A549 cell viability. The morphological changes of the cells were observed under a fluorescence microscope, and flow cytometry was employed for analyzing the cell apoptosis. The effects of matrine and the PI3K specific inhibitor LY294002 (10 nmol/L) on AKT pathway and autophagy-related proteins in A549 cells were investigated using Western blotting. RESULTS: Matrine significantly inhibited the proliferation of A549 cells in a time- and dose-dependent manner (P &lt; 0.05). At the concentration of 1.6 g/L or higher, matrine caused obvious cell shrinkage and fragmentation and significantly increased floating cells; autophagy vacuoles could be observed in the cells after acridine orange staining. Within the concentrations range of 0.8-1.6 g/L, matrine time- and dosedependently increased the cell apoptosis. Treatment of the cells with 1.6 g/L matrine and 10 nmol/L LY294002 resulted in significantly lowered expressions of p-AKT and p-mTOR proteins and increased the expression of light chain 3B (LC 3B), an autophagy-related protein, as compared with those in the control cells (P &lt; 0.05). CONCLUSIONS: We demonstrate that matrine inhibits the proliferation and induces autophagy and apoptosis of A549 cells by deactivating AKT pathway, suggesting the potential of matrine as an anti-cancer agent for lung cancer.",
              "AIMS: Obesity is not only associated with metabolic diseases but is also a symptom of menopause in women. To date, there are no effective drugs for the management of obesity, and it is important to find new agents with fewer side effects, for the treatment of obesity. This study aimed to determine the anti-obesity effect of 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), a heat shock protein 90 (Hsp90) inhibitor, and its underlying mechanism in rats with ovariectomy-induced obesity. MAIN METHODS: Ovariectomy (Ovx) rats were treated with 17-DMAG (1mgkg(-)(1), intraperitoneally) for eight weeks from one week after surgery. The body weight, food intake, locomotor activity, adipogenic- and autophagy-related protein expression in white adipose tissue (WAT) and plasma triglyceride (TG) levels were measured in sham and Ovx rats. KEY FINDINGS: Compared with sham rats, Ovx rats showed increased weight gain, food intake, WAT mass, TG levels, adipogenic protein expression, and decreased locomotor activity. Furthermore, autophagy-related proteins and Foxo3a of WAT were significantly increased in Ovx rats. However, with the exclusion of increased food intake, the changes induced by Ovx were all reversed in 17-DMAG-treated Ovx rats. In addition, the expression of Hsp70 and phosphorylation of Akt increased in 17-DMAG-treated Ovx rats. SIGNIFICANCE: These results suggest that 17-DMAG significantly ameliorated obesity induced by Ovx, and this phenomenon is accompanied by the downregulation of adipogenic-related and autophagy-related proteins as well as the upregulation of Akt-phosphorylation and Hsp70 expression. Therefore, 17-DMAG may be a potential agent for preventing or treating obesity in postmenopausal women.",
              "BACKGROUND: Autophagy is a conserved, highly-regulated catabolic process that plays important roles in growth, development and innate immunity in plants. In this study, we compared the rate of autophagy induction in Nicotiana benthamiana plants infected with Tobacco mosaic virus or the TMV 24A + UPD mutant variant, which replicates at a faster rate and induces more severe symptoms. Using a BirA* tag and proximity-dependent biotin identification (BioID) analysis, we identified host proteins that interact with the core autophagy protein, ATG8 in TMV 24A + UPD infected plants. By combining the use of a fast replicating TMV mutant and an in vivo protein-protein screening technique, we were able to gain functional insight into the role of autophagy in a compatible virus-host interaction. RESULTS: Our study revealed an increased autophagic flux induced by TMV 24A + UPD, as compared to TMV in N. benthamiana. Analysis of the functional proteome associated with ATG8 revealed a total of 67 proteins, 16 of which are known to interact with ATG8 or its orthologs in mammalian and yeast systems. The interacting proteins were categorized into four functional groups: immune system process, response to ROS, sulphur amino acid metabolism and calcium signalling. Due to the presence of an ubiquitin-associated (UBA) domain, which is demonstrated to interact with ATG8, the Huntingtin-interacting protein K-like (HYPK) was selected for validation of the physical interaction and function. We used yeast two hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and subcellular localization to validate the ATG8-HYPK interaction. Subsequent down-regulation of ATG8 by virus-induced gene silencing (VIGS) showed enhanced TMV symptoms, suggesting a protective role for autophagy during TMV 24A + UPD infection. CONCLUSION: This study presents the use of BioID as a suitable method for screening ATG8 interacting proteins in planta. We have identified many putative binding partners of ATG8 during TMV 24A + UPD infection in N. benthamiana plants. In addition, we have verified that NbHYPK is an interacting partner of ATG8. We infer that autophagy plays a protective role in TMV 24A + UPD infected plants.",
              "BACKGROUND: Nelson Bay orthoreovirus (NBV) was first isolated over 40 years ago from a fruit bat in Australia. Normally, NBV does not cause human diseases, but recently several NBV strains have been associated with human respiratory tract infections, thus attracting clinical attention. Autophagy, an evolutionarily conserved process in eukaryotic cells, degrades intracellular substrates, participates in multiple physiological processes, and maintains cellular homeostasis. In addition, autophagy is intimately involved in viral infection. METHODS: A new strain of NBV, isolated from a patient with a respiratory tract infection who returned to Japan from Bali, Indonesia, in 2007, was used in this study. NBV was rescued using a reverse genetics system involving cotransfection of BHK cells with 11 plasmids (pT7-L1 MB, pT7-L2 MB, pT7-L3 MB, pT7-M1 MB, pT7-M2 MB, pT7-M3 MB, pT7-S1 MB, pT7-S2 MB, pT7-S3 MB, pT7-S4 MB, and pcDNA3.1-T7), yielding NBV-MB. Recovered viruses were confirmed by immunofluorescence. The effect of NBV-MB on autophagy was evaluated by measuring the LC3-I/II proteins by immunoblot analysis after infection of BHK cells. Furthermore, after treatment with rapamycin (RAPA), 3-methyladenine (3-MA), chloroquine (CQ), or plasmid (GFP-LC3) transfection, the changes in expression of the LC3 gene and the amount of LC3-I/II protein were examined. In addition, variations in viral titer were assayed after treatment of BHK cells with drugs or after transfection with plasmids pCAGM3 and pCAGS3, which encode virus nonstructural proteins muNS and sigmaNS, respectively. RESULTS: NBV-MB infection induced autophagy in host cells; however, the level of induction was dependent on viral replication. Induction of autophagy increased viral replication. By contrast, inhibiting autophagy suppressed NBV replication, albeit not significantly. The NBV-MB nonstructural protein muNS was involved in the induction of autophagy with viral infection. CONCLUSIONS: NBV-MB infection triggered autophagy. Also, the NBV nonstructural protein muNS may contribute to augmentation of autophagy upon viral infection.",
              "Cadmium (Cd), an established carcinogen, is a risk factor for oral squamous cell carcinoma (OSCC). Macroautophagy/autophagy is proposed to play a pivotal role in Cd-mediated carcinogenic activity. However, the mechanisms underlying Cd-induced autophagy are poorly understood. In the present study, a CAL27 OSCC cell line exposed to 10(-6) M Cd for 8 weeks was used as a model system. Repeated Cd exposure induced significant migration and invasion of CAL27 cells. Furthermore, we showed that Cd increased the autophagic flux in CAL27 cells, as evidenced by the upregulation of LC3-II and the downregulation of P62/SQSTM1. The genetic blocking of autophagy inhibited Cd-induced migration and invasion, indicating a carcinogenic role of autophagy in Cd-treated CAL27 cells. Cd-induced NUPR1 expression, which contributes to lysosomal biogenesis and expression of autophagy-related gene, was found to mechanistically initiate autophagy in CAL27 cells. Of note, NUPR1 shRNA abolished Cd-induced autophagy both in vitro and in vivo. We also found that Cd triggered the generation of MDA in a xenograft tumour model and that N-acetyl-l-cysteine, a reactive oxygen species (ROS) scavenger, abrogated the effects of Cd on NUPR1-dependent autophagy in vivo. Taken together, these results demonstrate that ROS-dependent NUPR1-mediated autophagy plays an important role in repeated Cd exposure -induced cell growth, migration and invasion in OSCC cells.",
              "Autophagy is an evolutionarily conserved process that degrades and recycles defective organelles, toxic proteins, and various other aggregates on the cytoplasmic surface by sequestering them into autophagosomes which, then, fuse with lysosomes which degrade them. If these aggregates are not cleared, they accumulate and damage the cell resulting in cellular senescence and aging. Stem cells, with their capacity to differentiate, are crucial for tissue homeostasis. In addition to differentiation, the stemness of stem cells must be preserved. Recent studies in stem cells show the importance of autophagy in evading cellular senescence. In this review, we describe the conservative nature of the autophagy process, carried out throughout evolution. In particular, we highlight the role of autophagy in various evolutionarily diverse species and how it evolved to maintain tissue homeostasis and regulate aging and cellular senescence in stem cells.",
              "Objective: To observe the effect of tumor necrosis factor-alpha (TNF-alpha) monoclonal antibody on autophagy in allergic rhinitis (AR) mice. Methods: Thirty six weeks old BALB/c mice were randomly divided by random number table method into five groups: control group, model group (AR group), TNF-alpha antibody intervention group (AR+TNF-alpha group), autophagy inhibitor (3-methylindole, 3-NA) intervention group (AR+3-MA group), TNF-alpha antibody combined with autophagy inducer rapamycin (RAP) intervention group (AR+TNF-alpha+RAP group), with 6 mice in each group. AR model was established by conventional method, the corresponding reagent was administered before nasal cavity stimulation sensitization and during the whole experiment. Behavioral scores of mice were obtained, blood was collected from the eye socket, and mice in each group were sacrificed to collect nasal mucosa tissue samples. Pathological changes of nasal mucosa were observed by hematoxylin-eosin staining. Expression levels of inflammatory factor and IgE in serum were detected by enzyme-linked immunosorbent assay (ELISA). Expressions of autophagy related indicators microtubule-associated protein-1 light chain-3B (LC3B), Beclin-1, sequestosome1 (p62), autophagy-related 5 (ATG5), autophagy-related 7 (ATG7) were measured by Real-time PCR and Western blot. The aggregation of LC3B protein was observed by immunofluorescence. SPSS 19.0 software was used for statistical analysis. Results: Compared with the AR model group, symptoms of AR in AR+TNF-alpha group and AR+3-MA group were mild; the pathological changes of nasal mucosa were weak; the expression of IgE, TNF-alpha, interleukin 4 (IL-4), interferon-gamma (IFN-gamma) in serum significantly reduced (IgE: 666.19+/-78.35 (x+/-s) vs. 692.38+/-64.29 vs. 1 059.05+/-146.44, TNF-alpha: 112.06+/-12.95 vs. 113.17+/-15.43 vs. 161.22+/-17.96, IL-4: 54.05+/-7.14 vs. 58.26+/-5.67 vs. 79.95+/-6.33, IFN-gamma: 28.58+/-4.51 vs. 30.67+/-2.60 vs. 39.83+/-3.31, all P<0.05), and the expression of LC3B /, Beclin-1, ATG5, ATG7 in nasal mucosa significantly decreased, the expression of p62 significantly elevated. After intervention with autophagy inducer RAP, the therapeutic effect of TNF-alpha monoclonal antibodies on AR was antagonized. Conclusion: TNF-alpha monoclonal antibody significantly improves nasal symptoms in AR mice by inhibiting autophagy levels.",
              "Objective: To explore the role of autophagy in PM2.5-induced inflammation in human nasal epithelial cells and related mechanism. Methods: Human nasal epithelial cells were exposed to different concentration of PM2.5 for different times, and the expression levels of microtubule-associated protein-1 light chain-3 (LC3 ) and Beclin1 proteins were measured by Western blot. The typical autophagosome and autolysosome were observed by using transmission electron microscopy (TEM). To observe autophagic flux, mRFP-GFP-LC3 plasmid was transfected to nasal epithelial cells and the punctate staining of mRFP-GFP-LC3 were determined by confocal laser scanning microscope. The expression of inflammatory cytokines interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in cell culture supernatant were assessed by enzyme-linked immunosorbent assay (ELISA). To assess the role of autophagy in PM2.5-mediated inflammation, autophagy related gene Atg5 and Beclin-1 were silenced by siRNA knockdown, and inflammatory cytokines were analyzed.GraphPad Prism 6.0 was used for statistical analysis. Results: PM2.5 exposure increased the expression of LC3 and Beclin-1 proteins in a dose- (in PM2.5 group with concentration of 0, 15, 30, 60, 120 mug/ml, the expression of LC3 was 0.021+/-0.001(x+/-s), 0.037+/-0.002, 0.058+/-0.005, 0.075+/-0.006, 0.085+/-0.004, respectively, F=126.8, P<0.05; the expression of Beclin-1 was 0.002+/-0.000, 0.003+/-0.000, 0.005+/-0.000, 0.007+/-0.001, 0.008+/-0.001, respectively, F=137.3, P<0.05) and time-dependent manner (in PM2.5 group with exposure time of 0, 3, 6, 12, 24 h, the expression of LC3 was 0.160+/-0.007, 0.222+/-0.003, 0.251+/-0.015, 0.483+/-0.029, 0.585+/-0.035, respectively, F=215.3, P<0.05; the expression of Beclin-1 was 0.059+/-0.002, 0.080+/-0.002, 0.087+/-0.002, 0.183+/-0.007, 0.228+/-0.005, respectively, F=137.3, P<0.05) in human nasal epithelial cells. TEM analysis showed typical autophagosome and autolysosome in cells after PM2.5 exposure for 24 h. PM2.5 significantly increased the number of yellow and red dots representing autophagosomes and autolysosomes respectively, indicating autophagic flux was elevated. Moreover, PM2.5 enhanced the secretion of inflammatory cytokines such as IL-6 and TNF-alpha, which was dramatically prevented by Atg5-siRNA and Beclin-1-siRNA. Conclusion: Autophagy plays an important role in PM2.5-caused inflammation response in nasal epithelial cells, which can induce release of inflammatory factors such as IL-6 and TNF-alpha and advance the inflammatory reaction.",
              "BACKGROUND/AIMS: Excessive exposure to UV radiation negatively affects the human skin, characterized by photo-damage (premature aging & carcinogenesis). UV-B radiation causes about 90% of non-melanoma skin cancers by damaging de-oxy ribonucleic acids (DNA). We have previously reported that UV-B radiation induces skin photodamage through oxidative & Endoplasmic Reticulum (ER) stresses and Glycyrrhizic acid (GA), a natural triterpene, protects skin cells against such stresses. UV-B radiation elicits signalling cascade by activation of proteins involved in sensing, signalling, and repair process of DNA damage. In this study, we explored the effects & mechanisms of Glycyrrhizic acid (GA) against UV-B -induced photodamage using a well established cellular model. METHODS: We used primary human dermal fibroblasts as a cellular model. The cells were cultured in the presence or absence of GA for 3,6, & 24 h. Effect of UV-B was assessed by examining cell viability, cell morphology, oxidative stress, ER stress, DNA damage & cellular autophagy levels through biochemical assays, microscopy & protein expression studies. RESULTS: In this study, we have determined the effect of GA on autophagy mediated DNA damage response system as the main mechanism in preventing photodamage due to UV-B -irradiation to primary human dermal fibroblasts (HDFs). GA treatment to UV-B exposed HDFs, significantly inhibited cell death, oxidative & ER stress responses, prevented Cyclobutane Pyrimidine dimer (CPD) DNA adduct formation, and DNA fragmentation via modulation of UV-B induced autophagic flux. Present results showed that GA treatment quenched reactive oxygen species (ROS), relieved ER stress response, improved autophagy (6 hr's post-UV-B -irradiation) and prevented UV-B induced DNA damage. CONCLUSION: The present study links autophagy induction by GA as the main mechanism in the prevention of DNA damage and provides a mechanistic basis for the photoprotective effect of GA and suggests that GA can be potentially developed as a promising agent against UV-B induced skin photo-damage.",
              "AIMS: Sirtuins have been implicated in the aging process, however, the functions of SIRT2 in post-maturation aging of oocytes are not fully understood. The purpose of the present investigation was to assess the roles of SIRT2 in aged oocytes and mechanisms involved. MAIN METHODS: The fresh MII oocytes were aging in vitro, and treated with SIRT2 inhibitor (SirReal2), autophagy activator (Rapamycin), and autophagy inhibitor (3-Ma) for 24h, respectively. Oocyte activation, cytoplasmic fragmentation, and spindle defects, mitochondrial distribution, ROS levels, ATP production, mitochondrial membrane potential, and early apoptosis were investigated. Western blotting was performed to determine LC3-II accumulation, SQSTM1 degradation, and caspase-3 activity. KEY FINDINGS: SIRT2 expression gradually decreased in a time-dependent manner during oocyte aging. Treatment with SirReal2 significantly increased the rates of oocyte activation, cytoplasmic fragmentation, and spindle defects. In particular, the high ROS levels, abnormal mitochondrial distribution, low ATP production, and lost DeltaPsim were observed in SirReal2-exposed oocytes. Further analysis revealed that LC3-II accumulation and SQSTM1 degradation were induced by SIRT2 inhibition. By performing early apoptosis analysis showed that oocyte aging was accompanied with cellular apoptosis, and SIRT2 inhibition increased apoptosis rates of aged oocytes. Importantly, upregulating autophagy with Rapamycin could mimic the effects of SIRT2 inhibition on apoptosis by increasing caspase-3 activation, whereas downregulating autophagy with 3-MA could abolish those effects by blocking caspase-3 activation. SIGNIFICANCE: Our results suggest that SIRT2 inactivation is a key mechanism underlying of cellular aging, and SIRT2 inhibition contributes to autophagy-dependent cellular apoptosis in post-maturation oocytes.",
              "BACKGROUND: Extensive death of uninfected bystander neuronal cells is an important component of the pathogenesis of cytomegalovirus retinitis (CMV). Our previous results have shown that there is a functional relationship between autophagy and apoptosis during MCMV infection of retinal pigment epithelium (RPE). The purpose of this study was to determine whether autophagy plays a significant role in the death of retinal cells during MCMV retinitis. METHODS: The retinas of adult BALB/c mice were infected with MCMV via supraciliary injection. Rapamycin, a mTOR inhibitor, was injected to MCMV-infected BALB/c mice intraperitoneally. Immunohistochemistry and western blot were performed to observe the spread pattern of virus in retinas and the levels of targeted proteins. Plaque assay was performed to determine the virus titer in different groups. Since Atg5 is a key gene regulating autophagy, we bred Atg5(flox/flox); Nestin-Cre mice to deeply elucidate the role of autophagy during MCMV retinitis. Atg5(flox/flox); Nestin-Cre mice were genotyped and infected with MCMV. Immunohistochemistry was performed to observe the type of virus-infected cells and apoptosis in retinas during MCMV retinitis. RESULTS: In MCMV mouse model, MCMV infection in outer nuclear layer (ONL) and inner nuclear layer (INL) in the retinas caused cleaved caspase 3 positive apoptosis, which is not co-localized with early antigen (EA) positive virus infected cells in rapamycin treated group. Rapamycin treatment increased the levels of LC3B-II by inhibiting mTOR and decreased the levels of cleaved caspase-3 during MCMV retinitis. However, virus propagation was not affected by rapamycin. In Atg5(flox/flox); Nestin-Cre mice, RPE and glial cells were the main targets of viral infection, and number of EA positive retinal cells and TUNEL positive retinal cells was significantly increased compared to Atg5(flox/+); Nestin-Cre mice though there was no difference of virus propagation between Atg5(flox/flox); Nestin-Cre mice and Atg5(flox/+); Nestin-Cre mice. CONCLUSIONS: Autophagy protects retinal cells from MCMV infection induced apoptosis through mTOR-mediated signaling pathway.",
              "Chronic myeloid leukemia (CML) is a lethal malignancy, and the progress toward long-term survival has stagnated in recent decades. Pristimerin, a quinone methide triterpenoid isolated from the Celastraceae and Hippocrateaceae families, is well-known to exert potential anticancer activities. In this study, we investigated the effects and the mechanisms of action on CML. We found that pristimerin inhibited cell proliferation of K562 CML cells by causing G1 phase arrest. Furthermore, we demonstrated that pristimerin triggered autophagy and apoptosis. Intriguingly, pristimerin-induced cell death was restored by an autophagy inhibitor, suggesting that autophagy is cross-linked with pristimerin-induced apoptosis. Further studies revealed that pristimerin could produce excessive reactive oxygen species (ROS), which then induce JNK activation. These findings provide clear evidence that pristimerin might be clinical benefit to patients with CML.",
              "Mutations exclusively in equilibrative nucleoside transporter 3 (ENT3), the only intracellular nucleoside transporter within the solute carrier 29 (SLC29) gene family, cause an expanding spectrum of human genetic disorders (e.g., H syndrome, PHID syndrome, and SHML/RDD syndrome). Here, we identify adult stem cell deficits that drive ENT3-related abnormalities in mice. ENT3 deficiency alters hematopoietic and mesenchymal stem cell fates; the former leads to stem cell exhaustion, and the latter leads to breaches of mesodermal tissue integrity. The molecular pathogenesis stems from the loss of lysosomal adenosine transport, which impedes autophagy-regulated stem cell differentiation programs via misregulation of the AMPK-mTOR-ULK axis. Furthermore, mass spectrometry-based metabolomics and bioenergetics studies identify defects in fatty acid utilization, and alterations in mitochondrial bioenergetics can additionally propel stem cell deficits. Genetic, pharmacologic and stem cell interventions ameliorate ENT3-disease pathologies and extend the lifespan of ENT3-deficient mice. These findings delineate a primary pathogenic basis for the development of ENT3 spectrum disorders and offer critical mechanistic insights into treating human ENT3-related disorders.",
              "AIMS: Mitochondrial dysfunction has been regarded as one of the hallmarks of cerebral ischemia-reperfusion injury. In previous studies, we have provided evidence that the extracellular signaling pathway (ERK) 1/2 inhibitor PD98059 improved the neurological deficits by modulating antioxidant and anti-apoptotic activities in rats subjected to cardiac arrest/cardiopulmonary resuscitation (CA/CPR). Since oxidative stress can activate mitochondria-dependent apoptosis and autophagy, we further explored the effects of PD98059 on mitochondria involved with apoptosis and autophagy in rat CA model. MATERIALS AND METHODS: We disposed PD98059 in CA/CPR rats, tested the mitochondrial-mediated apoptosis pathway in brain tissues at 24h post-resuscitation by mitochondrial permeability transition pores (MPTP), cytochrome c (CytC), BCL-2, BAX, caspase-3, as well as autophagy by LC3, Beclin-1, and p62. Furthermore, we explored the relationship of dynamin-related protein 1 (Drp1) with apoptosis and autophagy. KEY FINDINGS: Our study showed that PD98059 decreased the openings of MPTP, CytC release, caspase3 activation, apoptotic indices, LC3-II, Beclin-1and increased P62. PD98059 also inhibited mitochondria-dependent apoptosis and the activity of autophagy in a dose-dependent manner in rat cerebral cortices at 24h post-resuscitation. The generation of phosphorylated Drp1-616 was down-regulated accompanied by a decrease of TUNEL-positive cells and LC3 in dual immunostaining after PD98059 inhibited activation of ERK signaling pathway in a dose-dependent manner in rat cerebral cortices at 24h post-resuscitation. SIGNIFICANCE: PD98059 protects the brain against mitochondrial-mediated apoptosis and autophagy at 24h post-resuscitation in rats subjected to CA/CPR, which is linked with the downregulation of Drp1 expression.",
              "AIMS: Sepsis is a leading cause of death and disability worldwide. Autophagy may play a protective role in sepsis-induced myocardial dysfunction (SIMD). The present study investigated whether valproic acid (VPA), a class I histone deacetylase (HDAC) inhibitor, can attenuate SIMD by accelerating autophagy. MAIN METHODS: A sepsis model was established via the cecum ligation and puncture of male Sprague-Dawley rats. Cardiac injuries were measured using serum markers, echocardiographic cardiac parameters, and hematoxylin and eosin staining. Cardiac mitochondria injuries were detected with transmission electron microscopy, adenosine triphosphate (ATP) and cardiac mitochondrial DNA (mtDNA) contents. Cardiac oxidative levels were measured using redox markers in the cardiac homogenate. Real-time polymerase chain reaction (RT-PCR) and Western blot were performed to detect the expression levels of relative genes and proteins. HDAC binding to the phosphatase and tensin homolog deleted on chromosome ten (PTEN) promoters and histone acetylation levels of the PTEN promoters were analyzed via chromatin immunoprecipitation and quantitative RT-PCR. KEY FINDINGS: VPA can ameliorate SIMD by enhancing the autophagy level of the myocardium to reduce mitochondrial damage, oxidative stress, and myocardial inflammation in septic rats. Moreover, this study demonstrated that VPA induces autophagy by inhibiting HDAC1- and HDAC3-mediated PTEN expression in the myocardial tissues of septic rats. SIGNIFICANCE: This study found that VPA attenuates SIMD through myocardial autophagy acceleration by increasing PTEN expression and inhibiting the AKT/mTOR pathway. These findings preliminarily suggest that VPA may be a potential approach for the intervention and treatment of SIMD.",
              "BACKGROUND: Neuroblastoma is the main solid extracranial tumor of childhood. The amplification of N-myc oncogene (MYCN) and 1p deletion are the main molecular alterations. These features are what make treatment impossible, especially in high-risk patients with metastases. MATERIALS AND METHODS: Our study investigated the processes undergone by CHP-212 neuroblastoma cells, after being treated with Casiopeinas(R) (Cas) IIgly, IIIEa, and IIIia for 2, 10, and 24 h. RESULTS: At 2 h, all the treatments Ied to apoptosis [defined by the presence of B-cell lymphoma 2 (BCL2), BCL2-associated X protein, cytochrome c, and caspase-3]. In addition, autophagy with specific molecules beclin-1 and microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/LC3-I (ratio >1). Later at 10 h, autophagy-associated proteins were observed, and at 24 h, only survival proteins nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-kappaB), and extracellular signal-regulated kinases (ERK)2/ERK1>1 were found. Another relevant finding was the presence of caspase-10 throughout the study, especially in cells treated with CasIIgly and CasIIIEa. CONCLUSION: These relationships indicate a possible mechanism of action of Casiopeinas on neuroblastoma.",
              "OBJECTIVE: Previous studies have indicated that the plasticizer di (2-ethylhexyl) phthalate (DEHP) affects lipid accumulation; however, its underlying mechanism remains unclear. We aim to clarify the effect of DEHP on lipid metabolism and the role of TYK2/STAT1 and autophagy. METHODS: In total, 160 Wistar rats were exposed to DEHP [0, 5, 50, 500 mg/(kg*d)] for 8 weeks. Lipid levels, as well as mRNA and protein levels of TYK2, STAT1, PPARgamma, AOX, FAS, LPL, and LC3 were detected. RESULTS: The results indicate that DEHP exposure may lead to increased weight gain and altered serum lipids. We observed that DEHP exposure affected liver parenchyma and increased the volume or number of fat cells. In adipose tissue, decreased TYK2 and STAT1 promoted the expression of PPARgamma and FAS. The mRNA and protein expression of LC3 in 50 and 500 mg/(kg*d) groups was increased significantly. In the liver, TYK2 and STAT1 increased compensatorily; however, the expression of FAS and AOX increased, while LPL expression decreased. Joint exposure to both a high-fat diet and DEHP led to complete disorder of lipid metabolism. CONCLUSION: It is suggested that DEHP induces lipid metabolism disorder by regulating TYK2/STAT1. Autophagy may play a potential role in this process as well. High-fat diet, in combination with DEHP exposure, may jointly have an effect on lipid metabolism disorder.",
              "OBJECTIVE: To investigate the protective effects of procyanidin on periprosthetic osteolysis caused by tricalcium phosphate (TCP) wear particles in the mouse calvaria and its mechanism. METHODS: Forty-eight male ICR mice were randomly divided into sham group, TCP group, and procyanidin (0.2 mg/kg, 1 mg/kg, 5 mg/kg)-treated group (n=12). A periprosthetic osteolysis model in the mouse calvaria was established by implanting 30 mg of TCP wear particles onto the surface of bilateral parietal bones following removal of the periosteum. On the 2(nd) day post-operation, procyanidin (1 mg/kg, 5 mg/kg) was locally injected to the calvaria under the periosteum every other day. After 2 weeks, all the mice were sacrificed to collect the blood samples and the calvaria. Periprosthetic osteolysis and osteoclastogenesis in the mouse calvaria were observed by tartrate resistant acid phosphatase (TRAP) staining and HE staining. mRNA levels of TRAP, capthesin K, c-Fos and NFATc1 in the periprosthestic bone tissue were examined by real-time fluorescence quantitative PCR. Serum contents of total anti-oxidation capacity (T-AOC) and MDA, and superoxide dismutase (SOD) activity were determined by chemical colorimetry. Protein expressions of autophagic biomarkers such as Beclin-1 and LC-3 in periprosthetic bone tissue of the calvaria were examined by Western blot. RESULTS: Compared with sham group, periprosthetic osteolysis, osteoclastogenesis, mRNA levels of TRAP, capthesin K, c-Fos and NFATc1, and serum MDA content were increased significantly in the TCP group (P0.05), whereas serum T-AOC level and SOD activity were decreased. The protein expressions of Beclin-1 and LC-3, and the conversion of LC3-II from LC3-I were both up-regulated markedly in the mouse calvaria of TCP group (P0.05). Compared with TCP group, osteolysis, osteoclastogenesis, mRNA levels of TRAP, capthesin K, c-Fos and NFATc1 and serum MDA content were decreased obviously in the procyanidine group (P0.05), serum T-AOC level and SOD activity were increased, the expressions of Beclin-1 and LC-3, and the conversion of LC3-II from LC3-I were down-regulated obviously in the mouse calvaria of procyanidin group (P0.05). CONCLUSION: Procyanidin has a protective effect of periprosthetic osteolysis caused by TCP wear particles in the mouse calvaia, its mechanism may be mediated by inhibition of oxidative stress and autophagy.",
              "OBJECTIVE: To investigate the effects of Notch signal on hypoxic induction factor (HIF-1alpha) and autophagy-associated genes Beclin1, LC3I, LC3II in oxygen-glucose deprivation (OGD) induced myocardial cell injury. METHODS: The OGD model was established using hypoxic culture box and hypoglycemic DMEM medium. The cells were divided into normal control group, OGD group, OGD + NC siRNA group, OGD + Notch1 siRNA group and OGD + HIF-1alpha siRNA group. Western blot was used to detect the interference effects of HIF-1alpha siRNA and Notch1 siRNA. The effects of Notch1 siRNA and HIF-1alpha siRNA on the activity of myocardial cells in OGD model were detected by the CCK-8 assay. The effects of Notch1 siRNA and HIF-1alpha siRNA on autophage-associated genes Beclin1, LC3I and LC3II expression were detected by Western blot. RESULTS: The results of Western blot showed that HIF-1alpha siRNA could effectively knock down the expression of HIF-1alpha in myocardial cells in OGD model, and Notch1 siRNA could effectively knock down the expression of Notch1 and HIF-1alpha in myocardial cells in OGD model. The result of CCK-8 assay showed that Notch1 siRNA and HIF-1alpha siRNA reduced the activity of myocardial cells in OGD model, and there was no statistical difference between the two groups. Western blot results showed that Notch1 siRNA and HIF-1alpha siRNA could reduce the expressions of the autophagy-associated genes Beclin1, LC3I and LC3II, and reduce the ratio of LC3II to LC3I at mRNA level. CONCLUSION: Notch1 plays a role in myocardial protection by regulating the expression of HIF-1alpha to regulate the autophagy in OGD model cells.",
              "As seen in other types of cancer, development of drug resistance in NSCLC treatment causes adverse effects on disease fighting process. Recent studies have shown that one of the drug resistance development mechanisms is that cancer cells may acquire the ability to escape from cell death. Therefore, development of anticancer drugs which have the strategy to redirect cancer cells to any cell death pathways may provide positive results for cancer treatments. Autophagy may be a target mechanism of alternative cancer treatment strategy in cases of blocked apoptosis. There is also a complex molecular link between autophagy and apoptosis, has not been fully understood yet. The dicyano compound which we used in our study caused cell death in NSCLC cell lines. When we analyzed the cells which were treated with dicyano compound by transmission electron microscope, we observed autophagosome structures. Upon this result, we investigated expression levels of autophagic proteins in the dicyano compound-treated cells by immunoblotting and observed that expression levels of autophagic proteins were increased significantly. The TUNEL assay and qRT-PCR for pro-apoptotic and anti-apoptotic gene expression, which we performed to assess apoptosis in the dicyano compound-treated cells, showed that the cell death does not occur through apoptotic pathway. We showed that the dicyano compound, which was developed in our laboratories, may play a role in molecular link between apoptosis and autophagy and may shed light on development of new anticancer treatment strategies.",
              "OBJECTIVE: To study the effect of beta-sheet blocking peptide H102 on the expression of AMPK-mTOR autophagy pathway-related protein in APP/PS1 double transgenic AD mice. METHODS: Thirty male APP/PS1 transgenic male AD mice of 6 months old were randomly divided into AD group and H102 intervention group, and C57BL/6J male mice of the same age were used as control group (n=15). The mice in the HF group were administered with 5 mul (5.8 mg/kg) of H102 polypeptide solution through the nasal cavity at the same time period, and the mice in the control group and the AD group were given the same amount of blank adjuvant solution daily. The memory recognition ability was tested by a new object recognition experiment 30 days after continuous administration. Immunohistochemistry and Western blot were used to detect the expressions of phosphorylated AMP-activated protein kinase(P-AMPK),phosphorylated mammalian target of rapamycin (P-mTOR) and ratio of LC32to LC31(LC3II/I )in brain tissue. RESULTS: Compared with the control group, the new object recognition index (RI) of the AD group was significantly lower (P0.05), and the P-AMPK and LC3II/I ratios in the brain of the mice were significantly lower (P0.05). The expression of P-mTOR protein was increased significantly (P0.05). Compared with the AD group, the RI of the H102 intervention group was increased significantly (P0.05), and the P-AMPK and LC3II/I ratios in the brain tissue of the mice were increased significantly (P0.05). The expression of P-mTOR protein was decreased significantly (P0.05). CONCLUSION: H102 can improve the recognition and memory ability of AD mice by activating the AMPK-mTOR autophagy-related pathway.",
              "The limited availability of rapid and reliable flow cytometry-based assays for ex vivo quantification of autophagy has hampered their clinical applications for studies of diseases pathogenesis or for the implementation of autophagy-targeting therapies. To this aim, we modified and improved the protocol of a commercial kit developed for quantifying the microtubule-associated protein 1A/1B light chain 3B (LC3), the most reliable marker for autophagosomes currently available. The protocol modifications were set up measuring the autophagic flux in neoplastic (THP-1 cells) and primary cells (peripheral blood mononuclear cells; PBMC) of healthy donors. Moreover, PBMC of active tuberculosis (TB) patients were stimulated with the Mycobacterium tuberculosis purified protein derivatives or infected with live Mycobacterium bovis bacillus Calmette-Guerin (BCG). We found that the baseline median fluorescent intensity (MFI) of THP-1 cells changed depending on the time of sample acquisition to the flow cytometer. To solve this problem, a fixation step was introduced in different stages of the assay's protocol, obtaining more reproducible and sensitive results when a post-LC3 staining fixation was performed, in either THP1 or PBMC. Furthermore, since we found that results are influenced by the type and the dose of the lysosome inhibitor used, the best dose of Chloroquine for LC3 accumulation were set up in either THP-1 cells or PBMC. Finally, applying these experimental settings, we measured the autophagic flux in CD14+ cells from active TB patients' PBMC upon BCG infection. In conclusion, our data indicate that the protocol modifications here described in this work improve the stability and accuracy of a flow cytometry-based assay for the evaluation of autophagy, thus assuring more standardised cell analyses.",
              "Sepsis is the leading cause of death in intensive care units worldwide; however, it remains a scientific and clinical challenge in modern medicine. An excessive inflammatory response associated with high level of reactive oxygen species results in mitochondrial dysfunction and activation of the unfolded protein response leading to subsequent energetic organ failure in septic patients. In addition to blocking the inflammatory cascade directly, new strategies focusing on host endogenous adaption to severe infection may hold better promise for improving outcomes in septic patients. Autophagy is a fundamental cellular response to stress and pathogen invasion. The study of autophagic responses to sepsis is a critical component of understanding the mechanisms by which tissues respond to infection. This review aims at elucidating the role of autophagy in sepsis-induced hepatic failure and further explores the possible factor that suppresses autophagy and potential targets of augmenting autophagy, in an effort to provide a new perspective for the clinical treatment of sepsis-induced hepatic failure.",
              "Oxidative-stress-induced senescence constitutes a great risk factor for chronic diseases. Therefore, ameliorating oxidative-stress-induced senescence is expected to prevent chronic diseases. The beneficial effects of bilberry anthocyanin (BA) on healthy aging were evaluated using 12 month old, aging female SD rats in this study. The experimental results suggested that consumption of a middle-dose of BA (MBA) appreciably increased the relative liver mass by 7.34% when compared with that of the AC group. Furthermore, BA significantly increased the total antioxidant capacity, total superoxide dismutase activity, and catalase activities; decreased malondialdehyde, serum low-density lipoprotein cholesterol (LDL-C), serum total cholesterol (TC), serum triglyceride (TG), and glycated serum protein (GSP) levels; and reduced TC/high-density lipoprotein cholesterol (HDL-C) and LDL-C/HDL-C ratios. In addition, MBA decreased the activity of fecal bacterial enzymes and increased the content of fecal short-chain fatty acids. The Western blot results showed that MBA significantly upregulated the expression of OCLN, ZO-1, and autophagy-related proteins (ATP6 V0C, ATG4D, and CTSB) in aging rats. Moreover, it also showed that MBA induced the phosphorylation of AMPK and FOXO3a and inhibited the phosphorylation of mTOR, which indicated that bilberry anthocyanin induced autophagy via the AMPK-mTOR signaling pathways. This induction of autophagy further promoted oxidative stress resistance effects and intestinal epithelial barrier function of bilberry anthocyanin in aging female rats.",
              "BACKGROUND: The emergence of multiple-antibiotic-resistant (MAR) Salmonella has been a serious threat worldwide. Salmonella can invade into host cells and evade the attacks of host humoral defenses and antibiotics. Thus, a new antibacterial agent capable of inhibiting intracellular Salmonella is highly needed. METHODS: The anti-intracellular activity and cytotoxicity of drugs on intracellular bacteria and macrophages were assayed using intracellular CFU assay and MTT cell viability assay, respectively. The uptake of gentamicin into macrophage and the effect of autophagy inhibitor on loxapine's anti-intracellular Salmonella activity were assessed by using image-based high-content system. The expression of bacterial genes was measured by real-time PCR. The efflux pump activity of bacteria was measured by Hoechst accumulation assays. RESULTS: With our efforts, an antipsychotic drug, loxapine, was identified to exhibit high potency in suppressing intracellular MAR S. Typhimurium, Staphylococcus aureus, Shigella flexneri or Yersinia enterocolitica. Subsequent investigations indicated that loxapine's anti-intracellular bacteria activity was not associated with increased penetration of gentamicin into bacteria and macrophages. Loxapine didn't inhibit bacterial growth in broth at concentration up to 500 muM and has no effect on Salmonella's type III secretion system genes' expression. Blockage of autophagy also didn't reverse loxapine's anti-intracellular activity. Lastly, loxapine suppressed bacterial efflux pump activity in all bacteria tested. CONCLUSION: Altogether, our data suggested that loxapine might suppress intracellular bacteria through inhibiting of bacterial efflux pumps. In light of its unique activity, loxapine represents a promising lead compound with translational potential for the development of a new antibacterial agent against intracellular bacteria.",
              "Hepatocellular carcinoma (HCC) has been reported to be one of the major tumors in the world. There is a study indicating that MCM3AP-AS1 is an oncogenic factor in HCC; however, the mechanism by which MCM3AP-AS1 regulates HCC remains not fully understood. Reverse Transcription-quantitative PCR and Western blot approaches were used to detect mRNA and protein levels of various genes. To examine invasion of HCC cells and lymphatic vessel formation of human dermal lymphatic endothelial cells (HDLECs), we employed transwell invasion assay and lymphatic vessel assay. Bioinformatic analysis and luciferase reporter assay were used to establish direct interactions between MCM3AP-AS1 and miR-455. Besides, The Cancer Genome Atlas analyses of HCCs were performed to determine the association of MCM3AP-AS1 and epidermal growth factor receptor (EGFR) with overall survival. MCM3AP-AS1 knockdown impaired invasion of HCC cells and lymphatic vessel formation of HDLECs. MCM3AP-AS1 directly interacted with miR-455. Furthermore, miR-455 inhibitor-transfected HepG2 cells enhanced the invasion and lymphatic vessel formation abilities. The rescue experiments indicated that EGFR was critical for MCM3AP-AS1- and miR-455-regulated invasion and lymphatic vessel formation. More interestingly, autophagy-related genes (Beclin1, LC3 II/I, and ATG7) were abnormally regulated in miR-455 mimic or inhibitor HepG2 cells. miR-455 mimic inhibited cell invasion and lymphatic vessel formation, which was evidently abrogated by ATG7 overexpression. Finally, we analyzed The Cancer Genome Atlas data sets to test the upregulated expression levels of MCM3AP-AS1 and EGFR. In addition, the results showed that low levels of both genes facilitate survival of HCC patients. In this study, we reveal a novel mechanism underlying MCM3AP-AS1-induced HCC metastasis by regulating miR-455. The conclusions provide more insights into understanding mechanism underlying HCC and help development of therapeutical approaches for treating HCC.",
              "Hepatitis C virus (HCV) and human immunodeficiency virus (HIV) are among the most dangerous pathogens globally. Infection with HCV has been reported in a high percentage of HIV patients. Viruses are obligate intracellular pathogens and their survival is associated with their capability to subvert antiviral defenses of cells and to improve cellular processes required for their replication. The aim of this study was to compare the expression rate of the key gene for autophagy process, Beclin-1, as a cellular response to viral infections, and its effect on IFN-alpha expression in both HCV and HCV/HIV patient groups. In this study, a total number of 40 samples of peripheral blood mononuclear cells (PBMCs) including 20 HCV and 20 HCV/HIV patients before treatment were evaluated. The HCV viral load in both groups was evaluated by semi quantitative real-time PCR. The level of Beclin-1 and IFN-alpha gene expression was examined in all samples by semi quantitative real-time PCR assay. The median viral load was 8.3x105 copies/ml in HCV group and 2.1x106 copies/ml in HCV/HIV patients. While the expression level of Beclin-1 gene in HCV group was significantly higher, the level of IFN-alpha expression was lower compared to the HCV/HIV group (P Keywords: autophagy; Beclin gene; HCV; HIV; IFN gene.",
              "Autophagy mediates the degradation of damaged proteins, organelles and pathogens, and plays a key role in health and disease. Thus, the identification of new mechanisms involved in the regulation of autophagy is of major interest. In particular, little is known about the role of lipids and lipid-binding proteins in the early steps of autophagosome biogenesis. Using target-agnostic, high-content, image-based identification of indicative phenotypic changes induced by small molecules, we have identified autogramins as a new class of autophagy inhibitor. Autogramins selectively target the recently discovered cholesterol transfer protein GRAM domain-containing protein 1A (GRAMD1A, which had not previously been implicated in autophagy), and directly compete with cholesterol binding to the GRAMD1A StART domain. GRAMD1A accumulates at sites of autophagosome initiation, affects cholesterol distribution in response to starvation and is required for autophagosome biogenesis. These findings identify a new biological function of GRAMD1A and a new role for cholesterol in autophagy.",
              "Previous study reported that either selective GSK-3beta inhibitor or up-regulating autophagy can alleviate cisplatin-induced ototoxicity. Other studies indicate that the activity of GSK-3beta is closely associated with the autophagy level. The purpose of this study is to primarily explore the role of autophagy in the alleviation effect of GSK-3beta inhibition on cisplatin-induced ototoxicity in vivo and in vitro. We observed the autophagy changes induced by GSK-3beta inhibitor in outer hair cells (OHCs) in a cisplatin-induced ototoxicity rat model. In addition, autophagy inhibitor 3-MA was used in vitro experiments to observe the influence of autophagy inhibition on the cell protection effect due to GSK-3beta inactivation. The relationship among autophagy, GSK-3beta and cell damage were inferred. Negative regulation of GSK-3beta significantly enhanced autophagy and alleviated cisplatin-induced hearing loss, OHC death in vivo and apoptosis in vitro. The autophagy inhibitor 3-MA inverted the protective effect of negative regulation of GSK-3beta. These results indicated that enhancing autophagy may be a key downstream effect of GSK-3beta inhibition in the alleviation of cisplatin-induced ototoxicity both in vivo and in vitro.",
              "Alcoholism is a serious addiction that can lead to various health complications such as liver fibrosis, steatosis, and cirrhosis. Carvacrol is present in many plant-based essential oils and used as a preservative in the food industry. In this study, we have investigated the hepatoprotective role of carvacrol against ethanol-induced liver toxicity in mice. To determine the effect of carvacrol on liver injury parameters, 5 doses of 50% ethanol (10mL/kg body weight) were orally administered every 12h for inducing the hepatotoxicity in experimental mice. Interestingly, carvacrol pre-treatment (50 and 100mg/kg) reversed the ethanol-induced effects on liver function, antioxidant markers, matrix metalloproteinases activities, and histological changes. Moreover, carvacrol binds to the active pocket of cytochrome P450 (Cyt P450) and inhibits its expression. Thus, our finding suggests carvacrol can be used as an adjuvant for the amelioration of alcohol-induced hepatotoxicity.",
              "Health risks have been closely related to increased exposure of fine particulate matter (PM2.5) in general population. Immune system is considered to be a most vulnerable target for airborne pollutants. PM2.5 could make some serious damages to the body organs by inducing immunotoxicity. However, the underlying molecular mechanisms are still unclear. The purpose of this study is aimed to elucidate the possible mechanisms of PM2.5-mediated immunotoxicity on spleen organ by using SD rat models. This research demonstrated that the spleen structure damage induced by PM2.5 treatment was more pronounced in winter than in summer. Mechanistically, TUNEL staining show a considerable increase in spleen apoptosis by summer and winter PM2.5 exposures compared with control. However, winter PM2.5 exposure caused more toxicity in the spleen than summer PM2.5 exposure. Furthermore, our results illustrated that PM2.5 triggered oxidative stress and ERS in spleen tissues of SD rats, and lead to apoptosis via upregulation of CHOP and caspase-12. Likewise, the protein levels of LC3 were significantly increased and p62 was decreased by PM2.5 exposure, thereby activated the autophagy of spleen in SD rats in a concentration-dependent manner. In conclusion, this study supported that PM2.5 mediated the immunotoxicity by the occurrence of stimulation of ERS and autophagy in SD rats. Taken together, these findings suggest PM2.5 as potential agent of immunotoxicity that needs an urgent attention. Graphical abstract Graphical abstract contains poor quality of text inside the artwork. Please do not re-use the file that we have rejected or attempt to increase its resolution and re-save. It is originally poor, therefore, increasing the resolution will not solve the quality problem. We suggest that you provide us the original format. We prefer replacement figures containing vector/editable objects rather than embedded images. Preferred file formats are eps, ai, tiff and pdf.Thanks you attention. We will provide tiff format image.",
              "Mitochondria play a crucial role in energy production, general cell metabolism, cell signaling, and apoptosis. Mitochondria are also the main source of reactive oxygen species, especially in the case of their dysfunction. Therefore, damaged or even superfluous mitochondria not required for normal cell functioning represent risk factors and should be removed in order to maintain cell homeostasis. Mitochondria removal occurs via mitophagy, a type of selective autophagy (from Greek autos, self and phagein, to eat) that takes place in parallel with mitochondrial biogenesis and other processes. This review outlines general views on autophagy and mitophagy and summarizes information on the autophagy-related (Atg) proteins and their complexes involved in these processes. Yeast, especially Saccharomyces cerevisiae, is a convenient model system for studying molecular mechanisms of mitophagy because yeast genome, transcriptome, and proteome have been well characterized and because genetic manipulations with yeast are relatively simple and fast. Furthermore, yeast contain a number of orthologs of human proteins. Mitophagy in yeast is promoted by various factors, such as starvation, aging, oxidative stress, mitochondrial dysfunction, signaling proteins, and modification of mitochondrial proteins. In this review, we discuss molecular mechanisms underlying mitophagy and its regulation in yeast and present examples of relationships between mitophagy and ubiquitination-deubiquitination processes, as well as between mitophagy and other types of autophagy.",
              "Kidney ischemia reperfusion injury (IRI) is an acute kidney injury associated with high number of mortality. We have examined the molecular mechanism and found that oxidative stress and hypoxia leads to induction of autophagy. In IRI induced autophagy, TFEB translocated to nucleus in response to IRI and induced a number of target genes of Coordinated Lysosomal Expression and Regulation (CLEAR) network. Real-time PCR analyses result showed IRI dependent increase in mRNA level to lysosomal hydrolases (Ctsa, Psap), lysosomal membranes (Lamp1), lysosomal acidification (Atp6ap1) non-lysosomal proteins involved in lysosomal biogenesis (M6pr, Nagpa) and autophagy (Becn1, VPS11). Overall, both lysosomal biogenesis and autophagy pathways were induced. Two key players of TFEB dependent proteins in autophagy, LAMP1 and BECN1 were verified by protein analyses. Pretreatment with urolithin A promoted autophagy and attenuated renal injury in kidney IRI and thus inverse relationship existed between TFEB-CLEAR pathway and kidney injury. Urolithin A also attenuated IRI induced pro-inflammatory cytokines TNFalpha, IL1beta, MIP1alpha and MIP2 mRNA and associated kidney injury. Overall, our results explored the understanding of autophagy and CLEAR network to kidney IRI and those insights may help to develop new therapeutic strategies to protect against IRI.",
              "AIMS: The aim of the present study is to shed light on the modulating action of selenium on two of the most crucial cellular pathways; apoptosis and autophagy and the possible interplay between them in determining the pituitary fate in the context of mercury intoxication through demonstration of the molecular, histopathological, immunohistochemical, and ultrastructural features of selenium mercury-treated adenohypophysis. METHODS: Thirty adult Sprague Dawley male albino rats were assigned into control group, mercury-treated group and mercuryselenium concomitantly-treated group. The adenohypophysis was subjected to structural, molecular and protein expression assessment of autophagy and apoptotic markers and western blotted analysis of Beclin 1 as a key cross-regulator of autophagy and apoptosis. KEY FINDINGS: Selenium treatment ameliorated the mercury-induced apoptosis detected by improvement in PCR and immunohistochemical expression of the apoptotic markers Bax, Bcl-2 and Caspase-3. Selenium also improved mercury-induced autophagic dysfunction with statistically significant improvement in western blotted levels of the autophagy markers LC3I, LC3II and Beclin1. The histopathological and ultrastructural studies strongly confirmed those findings. SIGNIFICANCE: The crosstalk between the apoptotic Bcl-2 family of proteins and the autophagic Beclin-1LC3 pathway in the context of mercury intoxication paves the way for developing novel effective treatment strategies for several mercury-induced pituitary diseases.",
              "Vascular endothelial growth factor C (VEGF-C) is an angiogenic and lymphangiogenic growth factor. Recent research has revealed the role for VEGF-C in regulating autophagy by interacting with a nontyrosine kinase receptor, neuropilin-2 (NRP-2). However, whether VEGF-C participates in regulating cell survival and autophagy in renal proximal tubular cells is unknown. To address this question, we employed a cell modal of serum deprivation to verify the role of VEGF-C and its receptor NRP-2 in regulating cell survival and autophagy in NRK52E cell lines. The results show that VEGF-C rescued the loss of cell viability induced by serum deprivation in a concentration-dependent manner. Furthermore, endogenous VEGF-C was knocked down in NRK52E cells by using specific small-interfering RNAs (siRNA), cells were more sensitive to serum deprivation-induced cell death. A similar increase in cell death rate was observed following NRP-2 depletion in serum-starved NRK52E cells. Autophagy activity in serum-starved NRK52E cells was confirmed by western blot analysis of microtubule-associated protein-1 chain 3 (LC3), immunofluorescence staining of endogenous LC3, and the formation of autophagosomes by electron microscopy. VEGF-C or NRP-2 depletion further increased LC3 expression induced by serum deprivation, suggesting that VEGF-C and NRP-2 were involved in controlling autophagy in NRK52E cells. We further performed autophagic flux experiments to identify that VEGF-C promotes the activation of autophagy in serum-starved NRK52E cells. Together, these results suggest for the first time that VEGF-C/NRP-2 axis promotes survival and autophagy in NRK52E cells under serum deprivation condition. SIGNIFICANCE OF THE STUDY: More researchers had focused on the regulation of autophagy in kidney disease. The effect of VEGF-C on cell death and autophagy in renal epithelial cells has not been examined. We first identified the VEGF-C as a regulator of cell survival and autophagy in NRK52E cell lines. And VEGF-C/NRP-2 may mediate autophagy by regulating the phosphorylation of 4EBP1 and P70S6K. VEGF-C treatment may be identified as a therapeutic target in renal injury repair due to its capacity to promote tubular cell survival in the future.",
              "Autophagy, a mechanism of recycling intracellular constituents, favors plant growth, especially under nutrient starvation. However, autophagy's role in regulating postharvest fruit senescence is unclear. Here, effects of the autophagy inhibitor hydroxychloroquine (HCQ) and activator LiCl on postharvest jujube fruit senescence were investigated. HCQ significantly reduced weight loss and decay incidence, and enhanced firmness compared with those of the control. LiCl had the opposite effects. Protein oxidation and H2O2 increased significantly in LiCl-treated compared with HCQ-treated fruit. The contents of vitamin C, total thiol, and phenolics, and total antioxidant capacity and DPPH-radical scavenging capacity, followed the order: HCQ>control>LiCl. The HCQ-mediated reduction in fruit respiration was significantly enhanced by ATP and partly reversed by 2,4-dinitrophenol, a mitochondrial uncoupler. Thus, jujube fruit senescence may be regulated by autophagy and the antioxidant capacity. A mechanism of autophagy-mediated postharvest fruit senescence involving mitochondrial biogenesis and respiration was proposed.",
              "BACKGROUND: Cytostatic treatment induces apoptosis or other types of cell death like autophagy, necrosis, mitotic catastrophe, etc. Autophagy can play a role in the drug resistance of neoplastic cells, allowing the survival of blast cells under stressful conditions, such as the use of cytostatics. Studies on apoptosis and autophagy 12-24 h after the start of treatment have not been conducted until now. OBJECTIVES: The study aimed to investigate the predictive and prognostic significance of autophagy and apoptosis in patients with acute myeloid leukemia (AML). MATERIAL AND METHODS: The study included 38 patients. Blood was collected before and 12-24 h after the start of treatment, since at that time point, the appropriate blast cell count was still available. Autophagy was measured with the expression of the ATG5, MAP1L3, LC3-I, and LC3-II proteins. The percentage of mononuclear cells in early and late apoptosis was evaluated with flow cytometry, using the annexin V and propidium iodide (PI) binding assay. RESULTS: The percentage of apoptotic blast cells before treatment was not associated with the response. However, in the remission group, the overall percentage of apoptotic cells measured 12-24 h after the start of treatment was higher than in non-remission patients, which was statistically significant. In neither group we found any difference in the level of autophagy before and 12-24 h after the start of treatment. Nevertheless, we observed an increasing tendency of the MAP1LC3 protein expression (not statistically significant) in the remission group 12-24 h after the start of treatment. Patients with a higher percentage of blast cells in apoptosis and with a higher expression of MAP1LC3 protein measured 12-24 h after the start of the therapy had longer overall survival (OS). CONCLUSIONS: A higher percentage of apoptotic as well as autophagic blast cells measured 12-24 h after the start of the chemotherapy is an independent factor associated with better outcomes.",
              "Temperature is a key factor for determining the lifespan of both poikilotherms and homeotherms. It is believed that animals live longer at lower body temperatures. However, the precise mechanism remains largely unknown. Here, we report that autophagy serves as a boost mechanism for longevity at low temperature in the nematode Caenorhabditis elegans. The adiponectin receptor AdipoR2 homolog PAQR-2 signaling detects temperature drop and augments the biosynthesis of two omega-6 polyunsaturated fatty acids, gamma-linolenic acid and arachidonic acid. These two polyunsaturated fatty acids in turn initiate autophagy in the epidermis, delaying an age-dependent decline in collagen contents, and extending the lifespan. Our findings reveal that the adiponectin receptor PAQR-2 signaling acts as a regulator linking low temperature with autophagy to extend lifespan, and suggest that such a mechanism may be evolutionally conserved among diverse organisms.",
              "BACKGROUND: Long non-coding RNA H19 (H19) plays an important role by regulating protein expression in different tissues and organs of the body. However, whether H19 induces hypoxia/reoxygenation (h/R) injury via increase of autophagy in the hepatoma carcinoma cells is unknown. RESULTS: H19 was expressed in the hepatoma carcinoma cells (Hep G2 and HCCLM3 cells) and its expression was most in 8 h/24R. The knockdown of H19 and 3-MA (an autophagy inhibitor) protected against h/R-induced apoptosis, cell damage, the expression of cleaved caspase-3 and cleaved caspase-9, the release of cytochrome c (Cyt c). The knockdown of H19 and 3-MA also decreased the autophagic vesicles (AVs) and the expression of Beclin-1 and the ration of LC3-II/LC3-I, and increased cell viability, the expression of Bcl-2 and P62 and the phosphorylation of PI3K, Akt and mTOR. In addition, chloroquine (CQ, an inhibitor of autophagy flux) markedly decreased formation of autophagy flux (the ration of LC3-II/LC3-I). The results of the knockdown of H19 group were similar to those of the 3-MA (or CQ) group. Rapamycin (a mTOR inhibitor, an autophagy activator) further down-regulated h/R-induced decrease of the phosphorylated PI3K, Akt and mTOR. The knockdown of H19 cancelled the effect of rapamycin. The overexpression of H19 further expanded h/R-induced increase of the ration of LC3-II/LC3-I and decrease of the phosphorylated PI3K, Akt and mTOR. CONCLUSIONS: Our results suggest that the long non-coding RNA H19 induces h/R injury by up-regulation of autophagy via activation of PI3K-Akt-mTOR pathway in the hepatoma carcinoma cells.",
              "Cadmium (Cd), as a kind of ubiquitous and highly toxic heavy metal pollutants, has been known to result in immunotoxicity in animals. As a multifunctional bioactivity disaccharide, trehalose (Tre) is characterized by antioxidative, antiapoptotic, and accelerating autophagy. In this study, Sprague-Dawley (SD) rats were fed with cadmium chloride (CdCl2) and/or Tre to explore the molecular mechanisms of Tre-protected against spleen injury caused by Cd exposure. Firstly, the results showed that Tre partially recovered splenic pathological changes induced by Cd exposure. Secondly, Tre dramatically declined the level of methane dicarboxylic aldehyde (MDA) and elevated the level of total antioxidant capacity (T-AOC) to weaken oxidative stress caused by Cd exposure in spleen tissue. Moreover, the results showed that Tre significantly suppressed Cd-induced the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and up-regulated the protein expression of nuclear Nrf2. Thirdly, Tre remarkably reduced the protein expression of sequestosome 1 (p62/SQSTM1) and microtubule-associated protein light chain 3II (LC-3II) to restore autophagy inhibition induced by Cd exposure. Finally, the results of TUNEL and the expression of apoptosis marker proteins showed that Tre significantly inhibited Cd-induced apoptosis in spleen tissue to exert its protective effects. In summary, the results indicated that Tre modulated Nrf2 signaling pathway, which interacted with apoptosis and autophagy to against Cd-induced spleen injury, providing potential therapeutic strategies for the prevention and treatment of Cd-related immune system diseases.",
              "Purpose: Disturbed metabolism of cholesterol and triacylglycerols (TGs) carries increased risk for coronary artery calcification (CAC). However, the exact relationship between individual lipid species and CAC remains unclear. The aim of this study was to identify disturbances in lipid profiles involved in the calcification process, in an attempt to propose potential biomarker candidates. Patients and methods: We studied 70 patients at intermediate risk for coronary artery disease who had undergone coronary calcification assessment using computed tomography and Agatston coronary artery calcium score (CACS). Patients were divided into three groups: with no coronary calcification (NCC; CACS: 0; n=26), mild coronary calcification (MCC; CACS: 1-250; n=27), or severe coronary calcification (SCC; CACS: >250; n=17). Patients' serum samples were analyzed using liquid chromatography-mass spectrometry in an untargeted lipidomics approach. Results: We identified 103 lipids within the glycerolipid, glycerophospholipid, sphingolipid, and sterol lipid classes. After false discovery rate correction, phosphatidylcholine (PC)(16:0/20:4) in higher levels and PC(18:2/18:2), PC(36:3), and phosphatidylethanolamine(20:0/18:2) in lower levels were identified as correlates with SCC compared to NCC. There were no significant differences in the levels of individual TGs between the three groups; however, clustering the lipid profiles showed a trend for higher levels of saturated and monounsaturated TGs in SCC compared to NCC. There was also a trend for lower TG(49:2), TG(51:1), TG(54:5), and TG(56:8) levels in SCC compared to MCC. Conclusion: In this study we investigated the lipidome of patients with coronary calcification. Our results suggest that the calcification process may be associated with dysfunction in autophagy. The lipidomic biomarkers revealed in this study may aid in better assessment of patients with subclinical coronary artery disease.",
              "The Kolliker's organ is a transient epithelial structure during cochlea development that gradually degenerates and disappears at postnatal 12-14 days (P12-14). While apoptosis has been shown to play an essential role in the degeneration of the Kolliker's organ, the role of another programmed cell death, autophagy, remains unclear. In our study, autophagy markers including microtubule associated protein light chain 3-II (LC3-II), sequestosome 1 (SQSTM1/p62) and Beclin1 were detected in the supporting cells of the Kolliker's organ through immunohistochemistry staining. In addition, Western blot and real-time PCR revealed a gradually decreased expression of LC3-II and an increased expression of p62 during early postnatal development. Compared to apoptosis markers that peaks between P7 and P10, autophagy flux peaked earlier at P1 and decreased from P1 to P14. By transmission electron microscopy, we observed representative autophagosome and autolysosome that packaged various organelles in the supporting cells of the Kolliker's organ. During the degeneration, these organelles were digested via autophagy well ahead of the cellular apoptosis. These results suggest that autophagy plays an important role in transition and degeneration of the Kolliker's organ prior to apoptosis during the early postnatal development.",
              "Octreotide (OCT) shows clinical efficacies in the treatment of liver cirrhosis complicated with gastrointestinal hemorrhage. Experiments were designed to investigate its function mechanism associated with endoplasmic reticulum stress (ERS)-induced autophagy and microRNA (miR). Protein associated with ERS and autophagy was detected by western blot. miR-101 was examined by qRT-PCR. Besides, miR-101 or G protein-coupled receptor 78 (GPR78)-silenced Caco-2 cells were established by transfection. Furthermore, western blot was used to determine TGF-beta activated kinase 1 (TAK1), AMPK, mTOR, p70S6K as well as their phosphorylated forms. Lipopolysaccharide (LPS) enforced the expression of GPR78. Besides, LPS triggered the production of Beclin-1 and LC3-II while mitigated the accumulation of p62. Then all these above results were reversed by OCT pretreatment. Moreover, miR-101 expression was downregulated by LPS while upregulated by OCT. Further, miR-101 knockdown strengthened ERS and promoted autophagy. GPR78 silence retarded autophagy process. In the end, OCT mitigated phosphorylation of TAK1, AMPK while enhanced the phosphorylated expression of mTOR and p70S6K in LPS-treated Caco-2 cells. The anti-autophagy property of OCT was mediated by miR-101-induced suppression of GPR78 in LPS-treated Caco-2 cells.",
              "A series of new quinoline derivatives was designed, synthesized and evaluated for their antiproliferative activity. The results demonstrated that compounds 11p, 11s, 11v, 11x and 11y exhibited potent antiproliferative activity with IC50 value of lower than 10muM against seven human tumor cell lines, and N-(3-methoxyphenyl)-7- (3-phenylpropoxy)quinolin-4-amine 11x was found to be the most potent antiproliferative agent against HCT-116, RKO, A2780 and Hela cell lines with an IC50 value of 2.56, 3.67, 3.46 and 2.71muM, respectively. The antitumor efficacy of the representative compound 11x in mice was also evaluated, and the results showed that compound 11x effectively inhibited tumor growth and decreased tumor weight in animal models. Further investigation on mechanism of action indicated that compound 11x could inhibit colorectal cancer growth through ATG5-depenent autophagy pathway. Therefore, these quinoline derivatives are a new class of molecules that have the potential to be developed as new antitumor drugs.",
              "Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium, contaminating in a wide variety of foods and feeds. Mycotoxins, including OTA, could cause immunosuppression in almost all previous studies in vivo. However, the vast majority of results in vitro showed that mycotoxins caused immunostimulation. Why the results of studies in vitro are contrary to studies in vivo is unknown. Our study aims to explore the underlying reason and mechanism of the paradoxical effect. In this study, porcine alveolar macrophage cell line 3D4/21 was chosen as an in vitro model and treated with 1.0 mug/mL OTA for different times. Some indexes, such as expression of inflammatory cytokines, migration, phagocytosis, macrophage polarization, autophagy-related proteins, and Akt1 phosphorylation, were detected. The results showed that pro-inflammatory cytokine expression, migration, and phagocytosis were increased, with macrophage polarization to the M1 phenotype at 24 h of OTA exposure. Surprisedly, anti-inflammatory cytokine expression was increased, cell phagocytosis and migration were decreased, and macrophage polarization was switched from M1 to M2 at 72 h of OTA exposure. Furthermore, we found that long-time exposure of OTA also suppressed autophagy, and the autophagy activator blocked the OTA-induced immunosuppression. Phosphorylation of Akt1 plays a positive role in autophagy inhibition. In conclusion, long-time instead of short-time exposure of OTA in vitro induced immunosuppression. The immunosuppression mechanism of OTA in vitro involved inhibition of autophagy through upregulating p-Akt1. Our results provide new insight into research on the mechanism of mycotoxin-induced immunosuppression in vitro.",
              "The aim of this study was to examine the effects of 5fluorouracil (5FU), antiepidermal growth factor receptor (EGFR) antibody and aspirin (ASA) on the characteristics of two CRC cell lines, HCT116 and HT29, maintained in a spherical culture system. We observed that the morphology of both the HCT116 and HT29 cellderived spheres was significantly impaired and the size of the colonospheres was markedly reduced following treatment with the aforementioned three drugs. In contrast to adherent cultures, the spherical cultures were more resistant to the tested drugs, as was reflected by their capacity to recreate the colonospheres when sustained in serumfree medium. Flow cytometric analysis of the drugtreated HCT116 cellderived spheres revealed changes in the fraction of cells expressing markers of cancer stem cells (CSCs), whereas the CSC phenotype of HT29 cellderived colonospheres was affected to a lesser extent. All reagents enhanced the percentage of nonviable cells in the colonospheres despite the diminished fraction of active caspase3positive cells following treatment of the HT29 cellderived spheres with antiEGFR antibody. Increased autophagy, assessed by acridine orange staining, was noted following the incubation of the HT29colonospheres with ASA and 5FU in comparison to the control. Notably, the percentage of cyclooxygenase (COX)2positive cells was not affected by ASA, although its activity was markedly elevated in the colonospheres incubated with antiEGFR antibody. On the whole, the findings of this study indicate that all the tested drugs were involved in different cellular processes, which suggests that they should be considered for the combined therapeutic treatment of CRC, particularly for targeting the population of CSClike cells. Thus, cancer cellderived spheres may be used as a preferable model for in vitro anticancer drug testing.",
              "Autophagy is a critical physiologic process contributing to the maintenance of cell homeostasis. Autophagy dysfunction has been directly linked to a growing number of neurodegenerative disorders, including prion diseases. However, little is known about the molecular mechanisms underlying autophagic failure and its connection with prion neuropathology. In a previous work we described alterations of this process in the central nervous system (CNS) of sheep naturally infected with classical scrapie, although specific neuronal populations such as Purkinje cells seemed to display an autophagy-related neuroprotective effect against prion toxicity. As atypical scrapie displays a lesion pattern different to the one observed in the classical form, using immunohistochemical analyses, we further investigated herein the role of autophagy in the CNS of sheep experimentally infected with atypical scrapie prions. While ATG5 protein showed a similar distribution in atypical scrapie to that observed in the classical form, expression of LC3-B and LC3-A did not change in any brain region. However, p62, a marker of impaired autophagy, was overexpressed in the most prion-affected areas, including Purkinje cells, which suggests that autophagic activity is deteriorated in the CNS of atypical scrapie and these cells are also susceptible to neurotoxicity and do not exhibit a general defensive mechanism based on autophagy. By comparing data from both clinical scrapie forms, we have demonstrated that autophagy impairment is highly dependent on the neuropathological lesion levels of the brain area analysed and may be implicated in prion neuropathology.",
              "E5 protein, the major oncoprotein of bovine Deltapapillomavirus (BPV), was found to be expressed in 18 of 21 examined urothelial cancers of cattle. E5 oncoprotein was found to interact with p62 which was degraded through the autophagosome-lysosome pathway as well as LC3-II and appeared to be involved in the phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 (eIF2alpha). Autophagy was morphologically documented by transmission electron microscope (TEM) through the detection of double-membrane autophagosomes and autolysosomes. Overexpression of Bag3 known to mediate selective autophagy was also demonstrated. Furthermore, Bag3 and BPV E5 oncoprotein were seen to co-localize with dynein and 14-3-3gamma, which suggested that Bag3 could be involved in inducing the retrograde transport of BPV E5 along microtubules to aggresomes, perinuclear sites with high autophagic flux. Electron dense perinuclear structures consistent with aggresomes were also documented by TEM in urothelial cancer cells. Finally, Bag3 was found to also interact with synaptopodin 2 (Synpo2), which would seem to contribute to cargo degradation as it has been shown to facilitate autophagosome formation. This study provides mechanistic insights into the potential role(s) of autophagy in BPV disease, which can help to develop future treatment and control measures for BPV infection. Activation of autophagy correlates positively with BPV infection and may play a role in biological behavior of bladder cancer as urothelial carcinomas of cattle are known to be characterized by a relatively low rate of metastasis.",
              "Exposure to urban airborne particulate matter (PM) associates with adverse health effects, but the exact mechanisms remain unclear. In this study, we focused on cytotoxicity (MTT), oxidative stress (DCF/FC), DNA damage (PI/FC), necrosis/apoptosis (FC), and autophagy (LC3 expression; WB/FC) triggered by urban dust (UD) in naive human alveolar epithelial A549 cells and in the cells with reduced glutathione (GSH). The A549 cells were grown in F12K/FCS media supplemented with coarse carbon black (CB; Huber990; 260 nm diameter; 200 mug.ml(-1)) or urban dust (UD; Standard Reference Materials; 200 mug.ml(-1)) for 24 h. To deplete intracellular glutathione (GSH), l-buthionine-(S,R)-sulfoximine (BSO; 100 mM; 24 h) was used. Pre-treatment with BSO depleted the cellular GSH by about 30%. A similar effect was noticed after UD. The CB was without any effects on the parameters tested, except for LC3 expression (autophagy) which increased by about twofold. However, UD decreased cell viability by about 27%, decreased cell proliferation in BSO pre-treated cells, increased ROS production, and increased both Hsp70 and LC3 proteins by about twofold, but most changes were unrelated to ROS-mediated GSH depletion. We conclude that urban dust-induced oxidative stress is important in PM toxicity, but other as yet unrecognized mechanisms are also involved.",
              "As a part of the aging process, N-retinylidene-N-retinylethanolamine (A2E) accumulates in the retina to activate autophagy in retinal pigmented epithelial cells. However, the effect of A2E photoactivation on autophagy, which is more clinically relevant, still remains unclear. Here, we investigated the effect of blue light (BL)-activated A2E on autophagy in human retinal pigmented epithelial cells, ARPE-19. A significant increase in LC3-II protein was observed when BL was irradiated on ARPE-19cells containing A2E. The mammalian target of rapamycin (mTOR) pathway was examined to verify whether autophagy was activated, but no change in AKT, mTOR, and 4EBP phosphorylation was observed. Transcription factor EB (TFEB) target gene expression, which is another pathway involved in autophagy, was also not altered by A2E and BL. However, intracellular p62 protein levels were significantly increased, which represented the inhibition of autophagic flux. To investigate the mechanism of the suppressed autophagic flux, the lysosomal state was observed. After BL irradiation, lysosomal damage was induced in A2E-treated ARPE-19cells, and this phenomenon was prevented by treatment with the antioxidant, N-acetylcysteine. Our results suggest that A2E photoactivation compromises autophagy in ARPE-19cells and that reactive oxygen species (ROS) play an important role in this process.",
              "Zearalenone (ZEA) can widely contaminate crops and agricultural products. The ingestion of ZEA-contaminated food or feed affects the integrity and functions of the intestines. In this study, we aimed to find the potential protective mechanism against ZEA ingestion. We found that ZEA induced cell death in IPEC-J2 cells. Meanwhile, the cytoprotective autophagy was activated in ZEA-treated cells. Further studies demonstrated that a p38/MAPK inhibitor down-regulated autophagy and increased cell death compared to those of the controls. Furthermore, ZEA could induce the accumulation of ROS, and eliminating ROS with NAC resulted in a decline in cell death, p38/MAPK phosphorylation, and the expression of LC3-II compared to those of ZEA-group. In addition, cytochrome P450 reductase (CYPOR) was significantly increased in ZEA-treated cells compared to that in the controls, and an inhibitor of CYPOR decreased ROS levels and mitigated cell death compared to those of the ZEA-group. More importantly, we found that blocking both p38/MAPK signalling and autophagy could enhance CYPOR expression and elevate ROS levels. Overall, our study indicated that the p38/MAPK pathway could activate protective autophagy in response to the CYPOR-dependent oxidative stress that was induced by ZEA in IPEC-J2 cells.",
              "OBJECTIVE: To investigate the expression of autophagy-related protein Beclin-1 and microtubule-associated protein 2 light chain 3 (LC3) in periodontal ligament cells in orthodontic tooth pressure areas. METHODS: Sixty male SD rats were randomly divided into a blank control group and nine experimental groups. In the experimental groups, 0.392 N orthodontic force was used to move the first right upper molars for 15 min, 30 min, 1 h, 2 h, 4 h, 12 h, 1 d, 3 d, or 7 d. The blank control group did not receive any treatment. The rats were euthanized. Changes in the morphology of the periodontal membrane in the pressure areas were observed through hematoxylin and eosin (HE) staining. The expression levels of Beclin-1 and LC3 were detected by immunohistochemical staining, and tartrate-resistant acid phosphatase (TRAP) staining was performed for the counting of osteoclasts. RESULTS: The HE stains showed that the hyalinization of the periodontal ligament appeared in the pressure areas after 1 day of exertion and was gradually aggravated. The immunohistochemical stains showed that the expression levels of Beclin-1 and LC3 in the experimental groups gradually increased, peaked after 1 h, and then gradually decreased. The expression levels peaked again after 1 d, then decreased to baseline levels at 7 d of exertion. Beclin-1 and LC3 were expressed in the osteoclasts. The TRAP stains indicated that the number of osteoclasts started to increase after 1 day. CONCLUSIONS: Autophagy may participate in the process of periodontal ligament reconstruction in orthodontic tooth pressure areas by mediating the hyalinization of periodontal ligament and affecting the biological effects of osteoclasts.",
              "Objective To investigate the mechanism of serine hydroxymethyl transferase 2 (SHMT2) inducing autophagy and promoting chemotherapy resistance in colon cancer cells. Methods TCGA database and real-time quantitative PCR were used to analyze the level of SHMT2 mRNA in colon cancer tissues. Western blot analysis and immunohistochemistry were used to detect the expression and distribution of SHMT2 in colon cancer tissues. Western blot analysis was performed to detect the SHMT2 protein levels of SW480, SW620, HCT116, CACO2, RKO, HCT8, HT15 and HT29 cells. After over-expression of SHMT2 in CACO2 colon cancer cells, MTT assay was used to detect cell viability, and annexin V-FITC/PI double labeling was used to detect the apoptosis of colon cancer cells induced by 5-fluorouracil (5-Fu). The autophagosomes of colon cancer cells were observed by transmission electron microscopy. The protein levels of LC3 II/I, P62, cleaved PARP (c-PARP), and cleaved caspase-3 (c-caspase-3) were examined by Western blot analysis. Signaling Phospho-Antibody Array and Western blot analysis were applied to analyze the phosphorylation level of AMPK/mTOR. Results SHMT2 was highly expressed in colon cancer tissues and cells. Over-expression of SHMT2 significantly increased cell viability and the ratio of LC3 II/ LC3 I. It was found that the phosphorylation level of AMPK was raised and the phosphorylation level of mTOR was reduced after the over-expression of SHMT2. Conclusion SHMT2 may induce autophagy by promoting AMPK phosphorylation and directly or indirectly inhibiting mTOR activity, thus leading to chemotherapy-induced apoptosis tolerance and resistance to chemotherapeutics.",
              "Zingerone (ZO), one of the active components of ginger (Zingiber officinale), is a phenolic alkanone with antioxidant, antiapoptotic, and anti-inflammatory properties. Cisplatin (CP) is a widely used chemotherapeutic drug for solid tumors, but its therapeutic use is limited due to dose-dependent nephrotoxicity. In the present study, we investigated the ameliorative effect of ZO against CP-induced nephrotoxicity. Intraperitoneal administration of single-dose CP (7 mg/kg body weight) on the first day enhanced kidney lipid peroxidation and reduced antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione (GSH). CP increased serum urea and creatinine levels and disrupted histological integrity while causing a decrease aquaporin 1 (AQP1) level in the kidney tissues. CP induced inflammatory responses by elevating the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), interleukin-33 (IL-33) and nuclear factor kappa B (NF-kappaB), and activities of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, it also caused oxidative DNA damage and activation of apoptotic pathway by increasing of 8-hydroxy-2'-deoxyguanosine (8-OHdG), p53, cysteine aspartate-specific protease-3 (caspase-3), and Bcl-2-associated x protein (bax) while decreasing B cell lymphoma-2 (Bcl-2). However, treatment with ZO at a dose of 25 and 50 mg/kg b.wt. for 7 days significantly decreased oxidative stress, apoptosis, inflammation, and histopathological alterations while increased AQP1 levels in the kidney tissue. The results of the current study suggested that ZO as an effective natural product attenuates CP-induced nephrotoxicity.",
              "Failure of autophagy induction results in the accumulation of abnormal mitochondria to cause neurodegenerative diseases. Artificial autophagy activation via the mitochondrial delivery of polyrotaxane with autophagy induced activity is achieved using a MITO-Porter, a nanodevice for mitochondrial delivery. This strategy can be applied to innovative research and therapy.",
              "BACKGROUND: Allium species are magnificently nutritious and are commonly used as a part of the diet in Iran. They have health enhancing benefits including anticancer properties due to the presence of numerous bioactive compounds. Herein, we investigated in vitro and in vivo anticancer properties of Allium bakhtiaricum extracts. METHODS: Anti-growth activity of different fractions was explored in vitro on different cancerous cells using MTT assay, Annexin V/PI and SA-beta-gal staining, Western blotting, flowcytometric and immunofluorescence microscopic evaluations. In vivo antitumor activity was investigated in BALB/c mice bearing 4 T1 mammary carcinoma cells. RESULTS: We demonstrated that chloroformic and ethyl acetate fractions exert cytotoxic activity toward MDA-MB-231 cells, the most sensitive cell line, after 72 h of treatment with IC50 values of 0.005 and 0.006 mg/ml, respectively. Incubation of MDA-MB-231 cells with (1/4) and (1/2) IC50-72h concentrations of each fraction resulted in a significant G2/M cell cycle arrest. (1/4) IC50-72h concentration of the chloroform fraction led to the disruption of polymerization in mitotic microtubules. Exposure of human breast cancer cells to different concentrations of the extracts at different incubation times did not induce apoptosis, autophagy or senescence. Our in vivo study revealed that administration of the chloroform extract at a dose of 1 mg/kg/day strongly suppressed mammary tumor progression and decreased the number of proliferative cells in the lung tissues indicating its anti-metastatic effect. CONCLUSION: Our findings imply that the chloroform fraction of Allium bakhtiaricum possesses the suppressive action on breast cancer through mitotic cell cycle arrest suggesting a mechanism associated with disturbing microtubule polymerization.",
              "Macroautophagy (referred to here as autophagy) degrades and recycles cytoplasmic constituents to sustain cellular and mammalian metabolism and survival during starvation. Deregulation of autophagy is involved in numerous diseases, such as cancer. Cancers up-regulate autophagy and depend on it for survival, growth, and malignancy in a tumor cell-autonomous fashion. Recently, it has become apparent that autophagy in host tissues as well as the tumor cells themselves contribute to tumor growth. Understanding how autophagy regulates metabolism and tumor growth has revealed new essential tumor nutrients, where they come from, and how they are supplied and used, which can now be targeted for cancer therapy.",
              "Human bocavirus 1 (HBoV1) refers to a human parvovirus causing acute respiratory tract infection in children. Bocaviruses encode an NP1 protein, which has 47% amino acid homology with NP1 of Minute Virus of Canines (MVC) and Bovine Parvovirus (BPV), but not with any protein of other parvoviruses. NP1 was found to induce apoptosis in Hela cells, which does not depend on viral replication and other protein expression. However, whether NP1 induces pulmonary cell death is unclear. In the present study, we investigate the impacts of NP1 on the autophagy and viability of A549 cells by expressing NP1. The plasmid containing NP1 gene was transfected into A549 cells. The apoptosis of A549 was evaluated by apoptosis detection kit and expression of caspase3. Cell viability and cell migration were detected by CCK8 kit and cell scratch test, respectively. The autophagy-related proteins and HMGB1 were detected by Western blot after NP1 expression in transfected cells. The real-time PCR was employed to detect HMGB1 mRNA. The secretory HMGB1 in supernatant of cell culture was measured by ELISA kit. The transient expression of NP1 did not induce apoptosis in A549 cells, but inhibited cell viability and migration. The expression of Beclin1 and LC3 II increased significantly and that of autophagy substrate P62 decreased dramatically upon transfection of NP1. The expression of NP1 reduced both levels of mRNA and protein HMGB1. The NP1 induced A549 autophagy was activated by STAT3 signaling pathway. HBoV1 NP1 induced autophagy in A549 cells by activating phosphorylation of STAT3 signaling pathway and inhibited A549 cell viability. This study provides insight into further elucidating the replication mechanism of HBoV1.",
              "Autophagy is crucial for immune defense against Mycobacterium tuberculosis (Mtb) infection. Mtb can evade host immune attack and survival within macrophages by manipulating the autophagic process. MicroRNAs (miRNAs) are small, non-coding RNAs that are involved in regulating vital genes during Mtb infection. The precise role of miRNAs in autophagy with the exits of Mtb remains largely unknown. In this study, we found miR-1958, a new miRNA that could regulate autophagy by interacting with 3'UTR of autophagy-related gene 5 (Atg5). In addition, Mtb infection triggered miR-1958 expression in RAW264.7 cells. What's more, miR- 1958 overexpression blocked autophagic flux by impairing the fusion of autophagosomes and lysosomes. Overexpression of miR-1958 reduced Atg5 expression and LC3 puncta while inhibition of miR-1958 brought an increase of Atg5 and LC3 puncta; the opposite results were observed in detection of p62. The survival of Mtb in RAW264.7 cells transfected with mimic of miR-1958 was enhanced. Taken together, our research demonstrated that a novel miR-1958 could inhibit autophagy by interacting with Atg5 and favored intracellular Mtb survival in RAW264.7 cells.",
              "FAM134B/RETREG1 is a selective ER-phagy receptor that regulates the size and shape of the endoplasmic reticulum. The structure of its reticulon-homology domain (RHD), an element shared with other ER-shaping proteins, and the mechanism of membrane shaping remain poorly understood. Using molecular modeling and molecular dynamics (MD) simulations, we assemble a structural model for the RHD of FAM134B. Through MD simulations of FAM134B in flat and curved membranes, we relate the dynamic RHD structure with its two wedge-shaped transmembrane helical hairpins and two amphipathic helices to FAM134B functions in membrane-curvature induction and curvature-mediated protein sorting. FAM134B clustering, as expected to occur in autophagic puncta, amplifies the membrane-shaping effects. Electron microscopy of in vitro liposome remodeling experiments support the membrane remodeling functions of the different RHD structural elements. Disruption of the RHD structure affects selective autophagy flux and leads to disease states.",
              "BACKGROUND: Atherosclerosis is a condition with the vascular accumulation of lipid plaques, and its main major contributing factor is endothelial injury induced by oxidized low-density lipoprotein (ox-LDL). Salidroside (SAL) is the primary active ingredient of Rhodiola rosea, and exhibits antioxidant properties on endothelial cells and alleviates atherosclerosis. However, the effect of SAL on autophagy in ox-LDL-induced vascular endothelial injury remains unclear. Here, we investigated the effect and underlying mechanisms of SAL on autophagy in human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were incubated with ox-LDL to induce in vitro atherosclerosis model. The cell viability and injury were evaluated by cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) release assay. The oxidative stress was evaluated by NADPH oxidase, malondialdehyde (MDA) and superoxide dismutase (SOD) activities. Immunofluorescence was performed to detect autophagy using LC3beta antibody. Quantitative real-time PCR (qRT-PCR) and western blot were performed to measure the mRNA expressions of SIRT1 and Forkhead box O1 (FOXO1). Nicotinamide (NAM) and AS1842856 were used to inhibit activities of SIRT1 and FOXO1, respectively. RESULTS: Exposure of HUVECs to ox-LDL (100 mug/mL) reduced cell viability, increased cellular MDA, and reduced SOD in a concentration-dependent manner. The pretreatment with SAL (20, 50 and 100 muM) significantly enhanced the cell viability and decreased LDH release in HUVECs exposed to ox-LDL (100 mug/mL). ox-LDL induced autophagy in HUVECs, which was further enhanced by pretreatment with SAL. However, SAL attenuated increase in oxidative stress in HUVECs induced by ox-LDL. ox-LDL reduced mRNA and protein expressions of SIRT1 and FOXO1, which could be reversed by SAL. The protective, anti-oxidative and pro-autophagic effects of SAL could be obviously abolished by cotreatment with SIRT1 inhibitor or FOXO1 inhibitor. CONCLUSION: Salidroside shows protective effect on endothelial cell induced by ox-LDL, and the mechanisms might be related to autophagy induction via increasing SIRT1 and FoxO1 expressions.",
              "AIMS: Coronary artery disease (CAD) ranks the leading cause of death globally. Interferon-gamma (IFN-gamma) gene, along with long noncoding RNA (lncRNA) BRAF-activated noncoding RNA (BANCR), could coordinately function in the occurrence of CAD. We hypothesized that level of IFN-gamma, genetic variants of IFN-gamma and BANCR gene should be associated with the occurrence of CAD. MATERIALS AND METHODS: A case-control study was conducted in Chinese population. KEY FINDINGS: We found that serum level of IFN-gamma in CAD cases was significantly higher than that in controls (P<0.001). Compared with the first quartile, all of the second (OR: 1.87; 95% CIs: 1.33-2.62), the third (OR: 1.79; 95% CIs: 1.30-2.45), and the fourth (OR: 3.98; 95% CIs: 2.59-6.12) quartiles of serum level of IFN-gamma were associated with increased risk of CAD (P<0.05). We found IFN-gamma gene (rs2069705 and rs2430561), and 2 variants in lncRNA BANCR (rs6559446 and rs79823312) could increase CAD susceptibility in allelic and dominant model, while IFN-gamma rs2069705 and rs2430561, BANCR rs79823312 were also associated with CAD risk in additive model. IFN-gamma rs2069705 and rs2430561 were associated with higher level of serum IFN-gamma in CAD patients (P<0.001). SIGNIFICANCE: This study confirmed the crucial role of IFN-gamma and lncRNA BANCR in the occurrence of CAD, and might serve as the biomarkers of CAD screening and prevention.",
              "OBJECTIVE: To investigate the inhibitory effect of genistein on activation of hepatic stellate cells (HSCs) in vitro and the role of the autophagy pathway regulated by PPAR-gamma in mediating this effect. METHODS: Cultured HSC-T6 cells were exposed to different concentrations of genistein for 48 h, and HSC activation was verified by detecting the expressions of -SMA and 1(I) collagen; autophagy activation in the cells was determined by detecting the expressions of LC3-II and p62 using Western blotting. The autophagy inhibitor 3-MA was used to confirm the role of autophagy in genistein-induced inhibition of HSC activation. A PPAR-gamma inhibitor was used to explore the role of PPAR-gamma in activating autophagy in the HSCs. RESULTS: Genistein at concentrations of 5 and 50 mumol/L significantly inhibited the expressions of -SMA and 1(I) collagen (P &lt; 0.05), markedly upregulated the expressions of PPAR-gamma and the autophagy-related protein LC3-II (P &lt; 0.05) and significantly down-regulated the expression of the ubiqutin-binding protein p62 (P &lt; 0.05) in HSC-T6 cells. The cells pretreated with 3-MA prior to genistein treatment showed significantly increased protein expressions of -SMA and 1(I) collagen compared with the cells treated with genistein only (P &lt; 0.05). Treatment with the PPAR-gamma inhibitor obviously lowered the expression of LC3-II and enhanced the expression p62 in genistein-treated HSC-T6 cells, suggesting the activation of the autophagy pathway. CONCLUSIONS: PPAR-gamma- regulated autophagy plays an important role in mediating genistein-induced inhibition of HSC activation in vitro.",
              "OBJECTIVE: To investigate whether autophagy mediates the effects of aldehyde dehydrogenase 2 (ALDH2) on the proliferation of neonatal rat cardiac fibroblasts cultured in high glucose. METHODS: Cardiac fibroblasts were isolated from neonatal (within 3 days) SD rats and subcultured. The fibroblasts of the third passage, after identification with immunofluorescence staining for vimentin, were treated with 5.5 mmol/L glucose (control group), 30 mmol/L glucose (high glucose group), or 30 mmol/L glucose in the presence of Alda-1 (an ALDH2 agonist), daidzin (an ALDH2 2 inhibitor), or both. Western blotting was employed to detect ALDH2, microtubule-associated protein 1 light chain 3B subunit (LC3B) and Beclin-1 in the cells, and a hydroxyproline detection kit was used for determining hydroxyproline content in cell culture medium; CCK- 8 kit was used for assessing the proliferation ability of the cardiac fibroblasts after the treatments. RESULTS: Compared with the control cells, the cells exposed to high glucose exhibited obviously decreased expressions of ALDH2, Beclin-1 and LC3B and increased cell number and hydroxyproline content in the culture medium. Treatment of the high glucose-exposed cells with Alda-1 significantly increased Beclin-1, LC3B, and ALDH2 protein expressions and lowered the cell number and intracellular hydroxyproline content, whereas the application of daidzin resulted in reverse changes in the expressions of ALDH2, Beclin-1 and LC3B, viable cell number and intracellular hydroxyproline content in high glucose-exposed cells. CONCLUSIONS: Mitochondrial ALDH2 inhibits the proliferation of neonatal rat cardiac fibroblasts induced by high glucose, and the effect is possibly mediated by the up-regulation of autophagy-related proteins Beclin-1 and LC3B.",
              "BACKGROUND/AIMS: Pyruvate kinase M2 (PKM2) is essential for aerobic glycolysis. Although high PKM2 expression is observed in various cancer tissues, its functional role in cancer metabolism is unclear. Here, we investigated the role of PKM2 in regulating autophagy and its associated pathways in prostate cancer cells. METHODS: Immunohistochemistry was performed to compare the expression level of PKM2 in prostate cancer patients and normal human, whereas expression of PKM2 in several cell lines was also examined by using western blot. PKM2 expression was silenced using various small interfering RNAs (siRNAs). Cell viability was examined using IncuCyte ZOOM live cell imaging system. Western blotting and immunofluorescence were performed to investigate the PKM2 knockdown on other cellular signaling molecules. Acridine orange and Monodansylcadaverine staining was performed to check effect of PKM2 knockdown on autophagy induction. High performance thin layer chromatography was carried out to quantify the level of different cellular metabolites (pyruvate and lactate). Colony formation assay was performed to determine the ability of a cells to form large colonies. RESULTS: PKM2 was highly expressed in prostate cancer patients as compared to normal human. PKM2 siRNA-transfected prostate cancer cells showed significantly reduced viability. Acridine orange, Monodansylcadaverine staining and western blotting analysis showed that PKM2 downregulation markedly increased autophagic cell death. Results of western blotting analysis showed that PKM2 knockdown affected protein kinase B/mechanistic target of rapamycin 1 pathway, which consequently downregulated the expression of glycolytic enzymes lactate dehydrogenase A and glucose transporter 1. Knockdown of PKM2 also reduced the colony formation ability of human prostate cancer cell DU145. CONCLUSION: To the best of our knowledge, this is the first study to show that PKM2 inhibition alters prostate cancer cell metabolism and induces autophagy, thus providing new perspectives for developing PKM2-targeting anticancer therapies for treating prostate cancer.",
              "The eukaryotic initiation factor 4E (eIF4E) is an emerging anticancer drug target for specific anticancer therapy as a promising approach to overcome drug resistance and promote chemotherapy antitumor efficacy. A series of bromophenol-thiazolylhydrazone hybrids were designed, synthesized and evaluated for their antitumor activities. Among of them, the most potent compound 3e (EGPI-1) could inhibit the eIF4E/eIF4G interaction. Further mechanism study demonstrated EGPI-1 played an antitumor role in multiple modes of action including regulating the activity of eIF4E by inhibiting the phosphorylation of eIF4E and 4EBP1, disrupting mitochondrial function through the mTOR/4EBP1 signaling pathway, and inducing autophagy, apoptosis and ROS generation. Moreover, EGPI-1 showed good safety and favorable pharmacokinetic properties in vivo. These observations demonstrate that EGPI-1 may serve as an excellent lead compound for the development of new anticancer drugs that target the eIF4E/eIF4G interface and as a chemical genetic probe to investigate the role of the eIF4E in biological processes and human diseases.",
              "As toxic elements when excessive, arsenic (As) and copper (Cu) are two naturally occurring elements that may be ingested by the organism at the same time. However, the precise damaged mechanism and the pathways that are activated by As and/or Cu is rarely researched in gizzard, a unique organ of birds. In this study, ultrastructural observations, TdT-mediated dUTP Nick-End Labeling, real-time quantitative PCR and Western blotting were performed to evaluate the toxic effects of chronic exposure to Cu(2+) and/or arsenite on chicken gizzard. The results revealed that increased apoptosis and autophagy levels induced by Cu(2+) and arsenite appeared to be independent of oxidative stress, which didn't have significant changes in different treatment groups at the same time point. Nevertheless, the redox balance gradually deviated with the extension of time. And increased mitochondrial division and decreased fusion were also caused by Cu(2+) and arsenite. In conclusion, apoptosis and autophagy in gizzard induced by Cu(2+) and/or arsenite, at least, strongly linked with the disruption of mitochondrial homeostasis. Our study showed that the combination of Cu(2+) and arsenite produces stronger toxicity. The results of this study can serve as a reference for agicultural feeding and environmental protection, that is, to avoid the combined exposure of Cu(2+) and arsenite to prevent greater economic losses and health risks.",
              "BACKGROUND: Elimination of malaria depends on mastering transmission and understanding the biological basis of Plasmodium infection in the vector. The first mosquito organ to interact with the parasite is the midgut and its transcriptomic characterization during infection can reveal effective antiplasmodial responses able to limit the survival of the parasite. The vector response to Plasmodium vivax is not fully characterized, and its specificities when compared with other malaria parasites can be of fundamental interest for specific control measures. METHODS: Experimental infections were performed using a membrane-feeding device. Three groups were used: P. vivax-blood-fed, blood-fed on inactivated gametocytes, and unfed mosquitoes. Twenty-four hours after feeding, the mosquitoes were dissected and the midgut collected for transcriptomic analysis using RNAseq. Nine cDNA libraries were generated and sequenced on an Illumina HiSeq2500. Readings were checked for quality control and analysed using the Trinity platform for de novo transcriptome assembly. Transcript quantification was performed and the transcriptome was functionally annotated. Differential expression gene analysis was carried out. The role of the identified mechanisms was further explored using functional approaches. RESULTS: Forty-nine genes were identified as being differentially expressed with P. vivax infection: 34 were upregulated and 15 were downregulated. Half of the P. vivax-related differentially expressed genes could be related to autophagy; therefore, the effect of the known inhibitor (wortmannin) and activator (spermidine) was tested on the infection outcome. Autophagic activation significantly reduced the intensity and prevalence of infection. This was associated with transcription alterations of the autophagy regulating genes Beclin, DRAM and Apg8. CONCLUSIONS: Our data indicate that P. vivax invasion of An. aquasalis midgut epithelium triggers an autophagic response and its activation reduces infection. This suggests a novel mechanism that mosquitoes can use to fight Plasmodium infection.",
              "BACKGROUND: Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation. RESULTS: In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/-II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1beta were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1beta. CONCLUSIONS: Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.",
              "BACKGROUND: Matrine, a traditional Chinese medicine, has been reported to exert anti-tumor effects in several types of cancers. Here, we explored the anti-tumor effects of matrine on the glioma cells. METHODS: Glioma cell line U251cells were treated with matrine to assess viability and proliferation using CCK8 and EdU assays. PI/FITC staining was performed for apoptosis assay. Transfections were performed for circRNA-104075 or Bcl-9 overexpression. Western blot analysis was performed to evaluate changes of protein levels and changes of gene level were detected by qRT-PCR in U251cells. RESULTS: Matrine suppressed cell viability while induced apoptosis and autophagy in U251cells. Matrine also decreased circRNA-104075 expression significantly. Overexpression of circRNA-104075 was found to counteract the inhibitory effects of matrine on cell proliferation and promoting effects on apoptosis and autophagy in U251cells. Moreover, the suppressed Wnt/beta-catenin and PI3K/AKT signaling pathways by matrine were activated by circRNA-104075 overexpression. Furthermore, Bcl-9 expression was also down-regulated by matrine treatment. Bcl-9 overexpression elevated the decreased cell proliferation while suppressed the increased apoptosis and autophagy induced by matrine in U251cells. CONCLUSION: Taken together, the present findings suggested that matrine induced apoptosis and autophagy through down-regulating circ-104075 and Bcl-9 expression via inhibition of PI3K/AKT and Wnt-beta-catenin pathways in glioma cells. The present study provides a foundation for further preclinical and clinical evaluations of matrine as a glioma therapy.",
              "As additive brominated fl ame retardants, hexabromocyclododecanes (HBCDs) are being widely used in diverse artificial materials and products, including thermal insulation building materials, housings of electronic equipment, and upholstery textiles. Toxicology studies have shown that HBCDs exposure are closely related to hepatotoxicity and liver diseases. The present study is designed to explore how HBCDs affect cell apoptosis and autophagy process in a human hepatocyte cell line (L02) and to reveal the underline molecular mechanisms. Firstly, HBCDs could elevate the apoptosis rate of L02cells dose-dependently. Three apoptosis related proteins (apoptotic protease activating factor 1 (Apaf-1), cysteinyl aspartate specific proteinase 3 (caspase-3) and cysteinyl aspartate specific proteinase 9 (caspase-9)) were observed to be up-regulated using western blotting method. Autophagy process was also started by HBCDs in L02cells as indicated by the increased expressions of LC3-phosphatidylethanolamine conjugate (LC3-II) and other autophagic protein markers (Beclin-1, autophagy related protein 3 (Atg3), autophagy related protein 5 (Atg5), autophagy related protein 7 (Atg7) and autophagy related protein 16L1(Atg16L1)). The results of the green fluorescent protein (GFP)-microtubule-associated protein 1 light chain 3 (LC3) intracellular localization and fluorescence intensity further evidenced the activation of autophagy in L02cells after treated with HBCDs. In addition, phosphatidylinositide 3-kinases/protein kinase B/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway was activated in L02cells by HBCDs, suggested by the increased expressions of related proteins. The inhibitors of PI3K (LY294002), DNA-activated protein kinase catalytic subunit (DNA-PKcs) (NU7441), Akt (MK2206), and mTOR (KU0063794) could obviously reduce the autophagic proteins prompted by HBCDs. The fluorescence intensities of GFP-LC3 transfected L02cells were also decreased significantly after the application of these inhibitors. These results indicated that PI3K/Akt/mTOR pathway was participated in regulating autophagy process promoted by HBCDs. In above, HBCDs could induce mitochondrial-dependent apoptosis and autophagy in L02cells, which was modulated by PI3K/Akt/mTOR pathway.",
              "OBJECTIVE: To investigate the effect on beta-Catenin pathway by lncRNA urothelial carcinoma associated 1 (UCA1) targeting regulated miR-185-5p in human lung adenocarcinoma A549 cell line. METHODS: A549 cell was selected as the study model and were divided into four groups, blank control group, sh-scramble negative control group (sh-scramble), sh-UCA1 interference group (sh-UCA1), miR-185 inhibitor group (miR-185 inhibitor) and sh-UCA1+ inhibitor group (sh-UCA1+inhibitor). The proliferation-, apoptosis- and autophagy- related protein levels were determined by Western blot. qRT-PCR was employed to detect the mRNA levels of UCA1 and miR-185-5p. The relationship between lnRNA UCA1 and miR-185-5p was validated by bioinformatics analysis and luciferase reporter system assays. BrdU staining was used to detect the cell growth, and immunofluorence staining was performed to measure the content of LC3(+) cells. RESULTS: sh-UCA1 significantly decreased UCA1 expression and increased miR-185-5p expression in A549 cells, and inhibited the cell growth and autophagy, while promoted the cell apoptosis (P<0.01). Bioinformatics analysis and luciferase reporter system assays demonstrated that lncRNA UCA1 and miR-185-5 can combine effectively, indicating that they have a targating relationship. sh-UCA1 also significantly inhibited the protein levels of beta-Catenin/TCF-4, Beclin 1 and LC3 , and decreased the cell growth and autophagy by the miR-185-5p; and down-regulated the LC3 expression (P<0.01). CONCLUSION: The effect of UCA1 inhibition for miR-185-5p was decreased by lncRNA UCA1 inference, and released the beta-Catenin/TCF-4, Beclin 1 and LC3 , and further reduced the autophagy and growth in A549 cells.",
              "MAIN CONCLUSION: Different autophagy pathways are a driver of vacuolar biogenesis and are development stage specific during the extrafloral nectary development in Citharexylum myrianthum. Plant autophagy plays an important role in various developmental processes such as seed germination, pollen maturation and leaf senescence. However, studies that address the evidence of autophagy and its role in the development of plant glands are scarce and largely restricted to laticifers. Regarding nectary, studies have repeatedly pointed to signs of degradation associated with the end of the secretory cycle, without exploring autophagy. Likewise, the relationship between autophagy and biogenesis of vacuoles remains an unexplored issue. In this study, using conventional and microwave fixation in association with ultracytochemical methods for transmission electron microscopy, we investigated the occurrence of autophagy and its implication in the differentiation of extrafloral nectary in Citharexylum myrianthum (Verbenaceae) under natural conditions, focusing on the vacuole biogenesis. We described a variety of vacuole types associated with the stage of nectary epidermis development, which differs with respect to origin, function and nature of the products to be stored. Three distinct autophagy pathways were detected: macroautophagy, microautophagy (both restricted to the undifferentiated epidermal cells, at the presecretory stage) and megaautophagy (circumscribed to the differentiated epidermal cells, at the postsecretory stage). Our study clearly demonstrated that the vacuole variety and autophagy processes in the nectary epidermal cells are development specific. This study highlights the role of autophagy in vacuole biogenesis and its implications for the development of nectary and opens new venues for future studies on regulation mechanisms for autophagy in plant secretory structures under normal conditions.",
              "Spinosad is one of the most extensively used bio-pesticide in the world. The effects of pesticide in human health are mainly associated with its residue in food or occupational exposure in agricultural production. The lung is the direct target of pesticides exposure, although the study of inhalation damage caused by Spinosad remains unclear. The aim of the present study was to evaluate the cytotoxic effects of the Spinosad in human lung cells. We demonstrated that Spinosad could inhibite the proliferation of human lung epithelial A549cells, induce the DNA damage and enhance the programmed cell death. Intracellular biochemical assay indicated that DNA double strand breaks, cleaved of PARP, release of cytochrome c, decrease of mitochondrial membrane potential, generation of reactive oxygen species (ROS), activation of caspase-3/9, increase of Bax/Bcl-2 ratio, LC3-II conversion, accumulation of Beclin-1, degradation of p62 and the changes in the phosphorylation of AMPK, mTOR are contributed to the toxic effects of Spinosad in A549cells. The results showed that the cytotoxicity of Spinosad may be associated with the activity of mitochondrial apoptotic pathways or AMPK/mTOR-mediated autophagy. Meanwhile, the DNA stand breaks caused by the Spinosad suggest it has a potential genotoxic effects on human lung cells. We conclude that Spinosad has a potential risk to human health by inducing the cytotoxic effects.",
              "Cellular senescence is a key factor driving age-related diseases. Recent studies have revealed that senescence-associated secretory phenotype, telomere attrition, epigenetic changes, and mitochondrial autophagy damage may mediate the pathogenesis of senescence-related idiopathic pulmonary fibrosis (IPF). Reducing the level of cellular senescence or clearing senescent cells can down-regulate the expression of fibrosis factors and alleviate the symptoms of IPF. In this review, we outlined the role and mechanism of cellular senescence in IPF.",
              "We previously found that genetic factors are associated with a familial predisposition for developing liver cirrhosis and hepatocellular carcinoma during chronic hepatitis B virus (HBV) infection. Autophagy has been shown to play a role in HBV replication and the course of disease. More than 190 host genes have been identified that modify the process of autophagy, but which of these genes are involved in chronicity of HBV infection and how this occurs remains unclear. Chronic hepatitis B (CHB) patients were recruited to investigate the expression of autophagy-modulating genes in peripheral blood mononuclear cells (PBMCs). mRNA prepared from PBMCs from members of two families with clustering HBV infection, including 11 CHB patients and nine healthy spouses, was hybridized to high-density oligonucleotide arrays. Immunoblot analysis was used to determine the level of autophagy. Of the 192 autophagy-modulating genes, 18 were found to be differently expressed. Of these, 11 displayed decreased expression in CHB patients, while seven displayed increased expression compared to those in healthy controls. Functional analysis showed that these genes are closely involved in initiation, nucleation, elongation of phagophores, formation of autophagosomes, transportation to lysosomes, and the process of degradation. Western blot analysis revealed inhibited autophagy in PBMCs based on decreased lipidation of LC3II. A differential expression profile of autophagy-modulating genes was observed, and decreased autophagy in PBMCs could be closely associated with chronicity of HBV infection, suggesting a novel strategy for the treatment of patients with chronic HBV infection.",
              "Lung cancer often has a poor prognosis, with brain metastases a major reason for mortality. We modified lonidamine (LND), an antiglycolytic drug with limited efficacy, to mitochondria-targeted mito-lonidamine (Mito-LND) which is 100-fold more potent. Mito-LND, a tumor-selective inhibitor of oxidative phosphorylation, inhibits mitochondrial bioenergetics in lung cancer cells and mitigates lung cancer cell viability, growth, progression, and metastasis of lung cancer xenografts in mice. Mito-LND blocks lung tumor development and brain metastasis by inhibiting mitochondrial bioenergetics, stimulating the formation of reactive oxygen species, oxidizing mitochondrial peroxiredoxin, inactivating AKT/mTOR/p70S6K signaling, and inducing autophagic cell death in lung cancer cells. Mito-LND causes no toxicity in mice even when administered for eight weeks at 50 times the effective cancer inhibitory dose. Collectively, these findings show that mitochondrial targeting of LND is a promising therapeutic approach for investigating the role of autophagy in mitigating lung cancer development and brain metastasis.",
              "Autophagy is an important intracellular process to maintain homeostasis and studies have shown the key role of autophagy in modulating the functions of reproductive system. Alongside with it, the activation of autophagy has also been found to regulate a number of important processes involved in in vitro fertilization including degeneration of granulosa cells and oocyte defects in obese and aging women; apoptosis of oocytes during vitrification-warming; quality and viability of embryo; developmental competence and pre-implantation development of in vitro produced blastocysts; placental vascularization and fetal growth. The different mechanisms that may contribute in autophagy-mediated increase in developmental competence and pre-implantation development include decrease in endoplasmic reticulum (ER) stress, activation of poly(ADP-ribosyl)ation (PARP) and reduction in free radical production. The present review discusses the role of autophagy activation in increasing the efficiency of in vitro fertilization by modulating different aspects related to fertilization.",
              "OBJECTIVES: Based on previous reports that ginsenosides have been shown to exert better preventive effects on cisplatin-induced kidney injury, the present work aims to evaluate the protective effects of ginsenoside Rb3 (G-Rb3) on cisplatin-induced renal damage and underlying mechanisms in vivo and in vitro. MATERIALS AND METHODS: The protective effect of G-Rb3 on cisplatin-induced acute renal failure in ICR mouse model and HEK293 cell model was investigated, and the underlying possible mechanisms were also explored. For animal experiment, renal function, kidney histology, inflammation, oxidative stress, relative protein molecules involved in apoptosis and autophagy signalling pathways were assessed. In addition, rapamycin (a specific inhibitor of mTOR), compound C (a specific inhibitor of AMPK) and acetylcysteine (NAC, a specific ROS scavenger) were employed to testify the effects of AMPK/mTOR signal pathway on the protective effects of G-Rb3 in HEK293 cells. RESULTS: Pre-treatment with G-Rb3 at doses of 10 and 20 mg/kg for ten days significantly reversed the increases in serum creatinine (CRE), blood urea nitrogen (BUN) and malondialdehyde (MDA), and decrease in glutathione (GSH) content and superoxide dismutase (SOD) activity. Histopathological examination further revealed that G-Rb3 inhibited cisplatin-induced nephrotoxicity. G-Rb3 diminished cisplatin-induced increase in protein expression levels of p62, Atg3, Atg5 and Atg7, and decrease in protein expression level of p-mTOR and the ratio of LC3-I/LC3-II, indicating that G-Rb3 suppressed cisplatin-induced activation of autophagy. Inhibition of autophagy induced inactivation of apoptosis, which suggested that autophagy played an adverse effect on cisplatin-evoked renal damage. Further, we found that G-Rb3 might potentially modulate the expressions of AMPK-related signal pathways. CONCLUSIONS: These findings clearly suggested that G-Rb3-mediated alleviation of cisplatin-induced nephrotoxicity was in part due to regulation of AMPK-/mTOR-mediated autophagy and inhibition of apoptosis in vitro and in vivo.",
              "Periodontal disease is a chronic infectious disease characterized by gingival inflammation and progressive destruction of alveolar bone, and the interaction between plaque microbes and host defense affects the process of the disease. Recent studies have found that autophagy is one of the most primitive host defense responses against pathogenic microbial invasion. Autophagy plays an important role in the development of periodontal disease through such functions as clearing pathogens, regulating inflammatory responses and promoting osteoclast activation to affect bone resorption with inflammatory factors. This review article mainly introduces the research advances on autophagy mechanism and the relationship between autophagy and periodontal disease from the domestic and foreign literatures and expounds the mechanism of autophagy and periodontal pathogens, inflammatory reaction and bone resorption of osteoclasts, so as to enrich the pathogenesis of periodontal disease and provide a new perspective for the treatment of periodontal disease.",
              "Eukaryotic cells use conserved quality control mechanisms to repair or degrade defective proteins, which are synthesized at a high rate during proteotoxic stress. Quality control mechanisms include molecular chaperones, the ubiquitin-proteasome system, and autophagic machinery. Recent research reveals that during autophagy, membrane-bound organelles are selectively sequestered and degraded. Selective autophagy is also critical for the clearance of excess or damaged protein complexes (e.g., proteasomes and ribosomes) and membrane-less compartments (e.g., protein aggregates and ribonucleoprotein granules). As sessile organisms, plants rely on quality control mechanisms for their adaptation to fluctuating environments. In this mini-review, we highlight recent work elucidating the roles of selective autophagy in the quality control of proteins and RNA in plant cells. Emphasis will be placed on selective degradation of membrane-less compartments and protein complexes in the cytoplasm. We also propose possible mechanisms by which defective proteins are selectively recognized by autophagic machinery.",
              "Objective: To investigate the changes of autophagy after spinal cord injury (SCI) in rats and its relationship with multisite phosphorylation of B-cell lymphoma-2 (Bcl-2) protein. Methods: Forty male Sprague-Dawley rats aged 8 weeks were used to prepare SCI models by modified Allen method, and the SCI model were prepared successfully in 36 rats. The 36 SCI models were randomly divided into SCI group, autophagy inhibitor group, and autophagy promoter group, with 12 rats in each group. Another 12 rats were selected as sham operation group with only laminectomy and no spinal cord injury. At the end of modeling, the autophagy inhibitor group and the autophagy promoter group were intrathecally injected with 20 muL of 600 nmol/L 3-methyladenine and 25 nmol/L rapamycin, respectively, once a day for 4 weeks. The sham operation group and the SCI group were injected with only 20 muL of normal saline at the same time point. The motor function of rat in each group was evaluated by the Basso-Beattie-Bresnahan (BBB) score at 1 day and 1, 2, 4 weeks after modeling. The rats in each group were sacrificed at 24 hours after the last injection and the spinal cord tissues were taken. ELISA assay was used to detect the levels of inflammatory factors in spinal cord tissues, including myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-alpha), and interleukin 1beta (IL-1beta); the morphological changes of spinal cord were observed by HE staining; the autophagy of mitochondria in spinal cord tissues was observed by transmission electron microscopy; the expressions of Beclin1 and microtubule-associated protein light chain 3 (LC3) were detected by immunofluorescence staining; neuronal apoptosis in spinal cord tissues were observed by TUNEL staining; LC3/TUNEL positive cells were calculated by immunofluorescence double staining; the expressions of Bcl-2 associated X protein (Bax), Bcl-2, p-Bcl-2 (Ser87), and p-Bcl-2 (Ser70) were detected by Western blot. Results: Compared with sham operation group, BBB score of SCI group decreased at each time point, while the levels of MPO, TNF-alpha, and IL-1beta increased; peripheral space of nerve cells enlarged, cells swelled, vacuoles appeared, and autophagic bodies appeared in mitochondria; the positive rates of Beclin1 and LC3 proteins, and apoptotic rate of neurons significantly increased; the LC3/TUNEL positive cells significantly increased; the expressions of Bax, p-Bcl-2 (Ser87), and p-Bcl-2 (Ser70) proteins increased, while the expression of Bcl-2 protein decreased; all showing significant differences ( P<0.05). Compared with SCI group, BBB score in autophagy inhibitor group decreased at each time point, while the levels of MPO, TNF-alpha, and IL-1beta increased; a few autophagic vesicles appeared in mitochondria; the positive rates of Beclin1 and LC3 proteins decreased and the apoptotic rate of neurons increased significantly; the LC3 positive cells decreased and the TUNEL positive cells increased; the expressions of Bax, p-Bcl-2 (Ser87), and p-Bcl-2 (Ser70) proteins increased, while the expression of Bcl-2 protein decreased. The results of autophagy promoter group were opposite to those of autophagy inhibitor group; all showing significant differences between groups ( P<0.05). Conclusion: Induction of autophagy after SCI in rats can reduce neuronal apoptosis and protect spinal cord function, which may be related to the inhibition of Bcl-2 protein multisite phosphorylation.",
              "Objective: To evaluate the effects of icariin on autophagy induced by low-concentration of glucocorticoid and exosome production in bone microvascular endothelial cells (BMECs). Methods: BMECs were isolated from femoral heads resected in total hip arthroplasty and then intervened with hydrocortisone of low concentration (0, 0.03, 0.06, 0.10 mg/mL), which were set as groups A, B, C, and D, respectively. On the basis of hydrocortisone intervention, 5x10 (-5) mol/L of icariin was added to each group (set as groups A1, B1, C1 and D1, respectively). Western blot was used to detect the expressions of microtubule-associated protein 1 light chain 3B (LC3B) and dead bone slice 1 (p62) after 24 hours. Exosomes were extracted from BMECs treated with icariin (intervention group) and without icariin (non-intervention group), and the diameter and concentration of exosomes were evaluated by nanoparticle tracking analysis technique. The total protein content of exosomes was detected by BCA method, and the expressions of proteins carried by exosomes including CD9, CD81, transforming growth factor beta 1 (TGF-beta 1), and vascular endothelial growth factor A (VEGFA) were assessed by Western blot. The BMECs were further divided into three groups: BMECs in the experimental group and the control group were co-cultured with exosomes secreted by BMECs treated with or without icariin, respectively; the blank control group was BMECs without exosome intervention. The three groups were treated with hydrocortisone and Western blot was used to detect the expressions of LC3B and p62. The scratching assay was used to detect cell migration ability; angiogenic ability of BMECs was also assessed. Results: With the increase of hydrocortisone concentration, the protein expression of LC3B- increased gradually, and the protein expression of p62 decreased gradually ( P<0.01). Compared with group with same concentration of hydrocortisone, the protein expression of LC3B- decreased and the protein expression of p62 increased after the administration of icariin ( P<0.01). The concentration of exosomes in the intervention group was significantly higher than that in the non-intervention group ( t=-10.191, P=0.001); and there was no significant difference in exosome diameter and total protein content between the two groups ( P>0.05). CD9 and CD81 proteins were highly expressed in the non-intervention group and the intervention group, and the relative expression ratios of VEGFA/CD9 and TGF-beta 1/CD9 proteins in the intervention group were significantly higher than those in the non-intervention group ( P<0.01). After co-culture of exosomes, the protein expression of p62 increased in blank control group, control group, and experimental group, while the protein expression of LC3B- decreased. There were significant differences among groups ( P<0.05). When treated with hydrocortisone for 12 and 24 hours, the scratch closure rate of the control group and experimental group was significantly higher than that of the blank control group ( P<0.05), and the scratch closure rate of the experimental group was significantly higher than that of the control group ( P<0.05). When treated with hydrocortisone for 4 and 8 hours, the number of lumens, number of sprouting vessels, and length of tubule branches in the experimental group and the control group were significantly greater than those in the blank control group ( P<0.05); the length of tubule branches and the number of lumens in the experimental group were significantly greater than those in the control group ( P<0.05). Conclusion: Icariin and BMECs-derived exosomes can improve the autophagy of BMECs induced by low concentration of glucocorticoid.",
              "This paper aimed to study the protective effect of ginsenoside Rg_1 on endotoxin(LPS)-induced apoptosis of lung epithelial cells and its mechanism of action. Mouse lung epithelial cells(MLE-12) were first treated with LPS. The autophagy changes and apoptosis and the relationship with concentration and time of LPS were observed. Then,the level of autophagy in MLE-12 was regulated at a specific concentration and action time of LPS,and the changes of apoptosis were observed. Secondly,ginsenoside Rg_1 and autophagy inhibitor 3-MA were added respectively at the same concentration and action time of LPS. The lung epithelial cells were grouped to observe the effect of ginsenoside Rg_1 on LPS-induced apoptosis of lung epithelial cells and its mechanism. In the animal experiment,the mice were grouped and tested by apoptosis protein,lung injury score and HE staining section to verify whether ginsenoside Rg_1 has a protective effect on LPS-induced lung injury. The results showed that apoptosis and autophagy increased as the rise of concentration after treatment with LPS for 12 h. The apoptosis increased gradually,and the autophagy increased first and then decreased over time at the LPS concentration of 25 g.L-1. The apoptosis of LPS group was higher than that of control group,and LPS+3-MA group increased further,while apoptosis decreased significantly in LPS+RAM(rapamycin,autophagy promoter) group. The autophagy increased in LPS group,decreased in LPS+3-MA group and increased in LPS+RAM group. The apoptosis of LPS group was higher than that of control group,and the apoptosis of LPS+Rg_1 group decreased. The apoptosis of LPS+Rg_1+3-MA group increased again. The autophagy of LPS group further increased after administration of ginsenoside Rg_1,but decreased after administration of 3-MA. In the in vivo experiments in mice,the apoptosis of LPS group increased significantly compared with the control group,while LPS + ginsenoside Rg_1 group decreased. Lung injury score and HE staining also conformed to the above trend. LPS can induce the apoptosis of lung epithelial cells in a time-dependent and concentration-dependent manner. The autophagy of lung epithelial cells increases with the rise of LPS concentration. At the specific concentration of LPS,autophagy increases first and then decreases after 12-16 hours. Proper increase of autophagy in lung epithelial cells within a certain period of time can reduce the apoptosis induced by LPS,while inhibition of autophagy can increase apoptosis. Ginsenoside Rg_1 has a protective effect on lung cancer epithelial cell apoptosis induced by autophagy.",
              "BACKGROUND: Glioblastoma is a disease with high heterogeneity that has long been difficult for doctors to identify and treat. ARHI is a remarkable tumor suppressor gene in human ovarian cancer and many other cancers. We found over-expression of ARHI can also inhibit cancer cell proliferation, decrease tumorigenicity, and induce autophagic cell death in human glioma and inhibition of the late stage of autophagy can further enhance the antitumor effect of ARHI through inducing apoptosis in vitro or vivo. METHODS: Using MTT assay to detect cell viability. The colony formation assay was used to measure single cell clonogenicity. Autophagy associated morphological changes were tested by transmission electron microscopy. Flow cytometry and TUNEL staining were used to measure the apoptosis rate. Autophagy inhibitor chloroquine (CQ) was used to study the effects of inhibition at late stage of autophagy on ARHI-induced autophagy and apoptosis. Protein expression were detected by Western blot, immunofluorescence and immunohistochemical analyses. LN229-derived xenografts were established to observe the effect of ARHI in vivo. RESULTS: ARHI induced autophagic death in glioma cells, and blocking late-stage autophagy markedly enhanced the antiproliferative activites of ARHI. In our research, we observed the inhibition of RAS-AKT-mTOR signaling in ARHI-glioma cells and blockade of autophagy flux at late stage by CQ enhanced the cytotoxicity of ARHI, caused accumulation of autophagic vacuoles and robust apoptosis. As a result, the inhibition of RAS augmented autophagy of glioma cells. CONCLUSION: ARHI may also be a functional tumor suppressor in glioma. And chloroquine (CQ) used as an auxiliary medicine in glioma chemotherapy can enhance the antitumor effect of ARHI, and this study provides a novel mechanistic basis and strategy for glioma therapy.",
              "Background: Ischemia/reperfusion (I/R) caused by ischemic stroke treatments leads to brain injury, and autophagy plays a role in the pathology. Astragaloside IV is a potential neuroprotectant, but its underlying mechanism on cerebral I/R injury needs to be explored. The objective of this study is to investigate the neuroprotective mechanism of Astragaloside IV against cerebral I/R injury. Methods: Middle cerebral artery occlusion method (MCAO) and oxygen and glucose deprivation/reoxygenation (OGD/R) method were used to simulate cerebral I/R injury in Sprague-Dawley (SD) rats and HT22 cells, respectively. The neurological score, 2,3,5-Triphe-nyltetrazolium chloride (TTC) staining, and transmission electron microscope were used to detect cerebral damage in SD rats. Cell viability and cytotoxicity assay were tested in vitro. Fluorescent staining and flow cytometry were applied to detect the level of apoptosis. Western blotting was conducted to examine the expression of proteins associated with autophagy. Results: This study found that Astragaloside IV could decrease the neurological score, reduce the infarct volume in the brain, and alleviate cerebral I/R injury in MCAO rats. Astragaloside IV promoted cell viability and balanced Bcl-2 and Bax expression in vitro, reduced the rate of apoptosis, decreased the expression of P62, and increased the expression of LC3II/LC3I in HT22 cells after OGD/R. Conclusions: These data suggested that Astragaloside IV plays a neuroprotective role by down-regulating apoptosis by promoting the degree of autophagy.",
              "AIMS: Bone marrow mesenchymal stem cells (BMSCs) show great potential in clinical applications such as in intervertebral disc degeneration. Nevertheless, environmental stress during the BMSC transplant or in the injured tissues is a catastrophic factor that causes cell toxicity and poor survival of BMSCs. Mitophagy plays a vital role in maintaining cellular homeostasis and defending against oxidative stress because this process could control mitochondrial quality and quantity by eliminating dysfunctional or damaged mitochondria that can cause cell death. However, the accurate mechanisms of mitophagy in protecting BMSCs against the harshness of oxidative stress remain largely unknown. MAIN METHODS: BMSCs were treated with H2O2 for various time periods. Mitophagy response was evaluated through the expression levels of LC3-II, p62 and mitophagosomal formation by using Western blot and fluorescence analysis. Cell apoptosis was examined by flow cytometry and TUNEL assay. The interactions of mitophagy and apoptosis and the possible signalling pathways were investigated through the co-treatment of mitophagy inhibitor or mitophagy activator with H2O2. KEY FINDINGS: Oxidative stress rapidly facilitated mitophagy through JNK at an early stage but decreased mitophagy and increased apoptosis at a late stage. Furthermore, mitophagy inhibition significantly enhanced the apoptosis in the cells treated by H2O2. SIGNIFICANCE: Induced mitophagy may play pivotal roles in protecting cells against oxidative stress in BMSCs.",
              "Autophagy is an evolutionarily conserved process in eukaryotes to maintain cellular homeostasis under environmental stress. Intracellular control is exerted to produce energy or maintain intracellular protein quality controls. Autophagy plays an important role in embryogenesis, implantation, and maintenance of pregnancy. This role includes supporting extravillous trophoblasts (EVTs) that invade the decidua (endometrium) until the first third of uterine myometrium and migrate along the lumina of spiral arterioles under hypoxic and low-nutrient conditions in early pregnancy. In addition, autophagy inhibition has been linked to poor placentation-a feature of preeclamptic placentas-in a placenta-specific autophagy knockout mouse model. Studies of autophagy in human placentas have revealed controversial results, especially with regard to preeclampsia and gestational diabetes mellitus (GDM). Without precise estimation of autophagy flux, wrong interpretation would lead to fixed tissues. This paper presents a review of the role of autophagy in pregnancy and elaborates on the interpretation of autophagy in human placental tissues.",
              "Selenocyanates and diselenides are potential antitumor agents. Here we report two series of selenium derivatives related to selenocyanates and diselenides containing carboxylic, amide and imide moieties. These compounds were screened for their potency and selectivity against seven tumor cell lines and two non-malignant cell lines. Results showed that MCF-7cells were especially sensitive to the treatment, with seven compounds presenting GI50 values below 10muM. Notably, the carboxylic selenocyanate 8b and the cyclic imide 10a also displayed high selectivity for tumor cells. Treatment of MCF-7cells with these compounds resulted in cell cycle arrest at S phase, increased levels of pJNK and pAMPK and caspase independent cell death. Autophagy inhibitors wortmannin and chloroquine partially prevented 8b and 10a induced cell death. Consistent with autophagy, increased Beclin1 and LC3-IIB and reduced SQSTM1/p62 levels were detected. Our results point to 8b and 10a as autophagic cell death inducers.",
              "Fluorene-9-bisphenol (BHPF), a substitute for bisphenol A, is a chemical component of plastics for industrial production. There is evidence that BHPF exerts an antioestrogenic effect on mice, induces endometrial atrophy and leads to adverse pregnancy outcomes. However, the effects of BHPF on oocyte maturation and ovary development as well as its possible mechanisms remain unclear. The objective of this study was to investigate the toxicity and mechanism of BHPF exposure in mouse oocytes in vitro and in vivo. Our results showed that BHPF could inhibit the maturation of oocytes in vitro by reducing the protein level of p-MAPK and destroying the meiotic spindle. We found that in vitro, BHPF-treated oocytes showed increased ROS levels, DNA damage, mitochondrial dysfunction, and expression of apoptosis- and autophagy-related genes, such as Bax, cleaved-caspase 3, LC 3 and Atg 12. In addition, in vivo experiments showed that BHPF exposure could induce the expression of oxidative stress genes (Cat, Gpx 3 and Sod 2) and apoptosis genes (Bax, Bcl-2 and Cleaved-caspase 3) and increase the number of atresia follicles in the ovaries. Our data showed that BHPF exposure affected the first polar body extrusion of oocytes, increased oxidative stress, destroyed spindle assembly, caused DNA damage, altered mitochondrial membrane potentials, induced apoptosis and autophagy, and affected ovarian development.",
              "Icariin is a flavonoid derived from Epimedium sagittatum, and has a wide range of biological and pharmacological effects; however, little is known regarding its effect on drugresistant ovarian cancer and the signal transduction pathways underlying the regulation of apoptosis and autophagy. The present study aimed to investigate the resensitization effects of icariin exerted on an ovarian cancer cell line. Autophagy was analyzed in a SKVCR cell line that had been treated with icariin. We investigated the sensitivity of SKVCR cells to cisplatin, as well as the effects of an autophagy agonist (rapamycin) on autophagy, apoptosis, and the protein kinase B (AKT) signaling pathway. Finally, the mechanism underlying the effects of autophagyrelated (ATG) protein ATG5 overexpression on autophagy, apoptosis and AKT signaling in SKVCR cells were determined. The results revealed that treatment with icariin inhibited cell viability and autophagy, but promoted G0/G1 phase cell cycle arrest and apoptosis as determined by Cell Counting Kit8, immunofluorescence and flow cytometry assays, respectively. Icariin reduced the resistance of SKVCR cells to cisplatin in vitro by inducing G1/S cell cycle transition, apoptosis and inhibiting autophagy. Furthermore, enhanced autophagy induced by rapamycin treatment or overexpression of ATG5 partially reversed the effect of icariin on cisplatin resistance and autophagy in SKVCR cells. At the molecular level, rapamycin treatment or overexpression of ATG5 reversed the effects of icariin on the expression of autophagyassociated proteins, including microtubuleassociated protein 1 light chain 3beta, Beclin1, ATG5 and p62, and the AKT/mammalian target of rapamycin (mTOR) pathway. Collectively, our results suggested that icariin enhances the chemosensitivity of SKVCR cells by suppressing autophagy via activation of the AKT/mTOR signaling pathway.",
              "Cardiac dysfunction is a major component of sepsis-induced multiorgan failure in critical care units. Uncoupling protein 2 (UCP2) involves immune response, regulation of oxidative stress, and maintenance of mitochondrial membrane potential as well as energy production. However, whether and how UCP2 plays roles in the development of septic cardiac dysfunction are largely unknown. Here, intraperitoneal injection of LPS significantly activated UCP2 expression accompanied by a significant decrease of cardiac function and caused a significantly lower survival rate in mice. Of note, knockdown of UCP2 through a cardiotropic adenoassociated viral vector carrying a short hairpin RNA (shRNA) specifically targeting the UCP2 evoked resistance to LPS-triggered septic cardiac dysfunction and lethality in vivo. Moreover, UCP2 deficiency ameliorated the reduced levels of intracellular ATP in the LPS-challenged heart tissues and preserved mitochondrial membrane potential loss in primary adult mouse cardiomyocytes in LPS-challenged animals. Mechanistically, we confirmed that the inhibition of UCP2 promoted autophagy in response to LPS, as shown by an increase in LC3II and a decrease in p62. At last, the autophagy inhibitor 3-MA abolished UCP2 knockdown-afforded cardioprotective effects. Those results indicate that UCP2 drives septic cardiac dysfunction and that the targeted induction of UCP2-mediated autophagy may have important therapeutic potential.",
              "Expansion of the autophagosomal membrane requires a mechanism to supply lipids while excluding most membrane proteins. In this issue, Valverde et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201811139) identify ATG2, a member of the autophagy-related protein family, as a lipid transfer protein and provide important novel insights on how autophagosomes grow.",
              "Photodynamic therapy (PDT) of cancer uses photosensitizers (PS), a light source and oxygen to generate high levels of reactive oxygen species (ROS), that exert a cytotoxic action on tumor cells. Recently, it has been shown that mixed non-symmetrical diaryl porphyrins, with two different pendants, are more photodynamically active than symmetrical diaryl porphyrins. In the present study, we investigate the in vitro photodynamic effects of four novel non-symmetrical diaryl porphyrins, two of which bear one pentafluoro-phenyl and one bromo-alkyl (apolar) pendant, whereas the two others bear one pentafluoro-phenyl and one cationic pyridine pendant. The four compounds were tested in a small panel of human cancer cell lines, and their photodynamic activities were compared with that of m-THPC (Foscan), currently the most successful PS approved for clinical use in cancer PDT. The results of the cytotoxicity studies indicate that the two molecules bearing the cationic pendant are more potent in vitro than those with the apolar pendant, and that they are as potent as Foscan. To gain further insights into the mechanism of PS-induced phototoxicity, induction of apoptotic, autophagic and necrotic cell death, and generation of reactive oxygen species (ROS) were evaluated in cancer cells following exposure to the PSs and irradiation. The effect of the PSs on the migratory activity of the cells was also assessed. The data obtained from this work support a greater potency of diaryl porphyrins with a positive charge in inducing cell death, as compared to those with the bromo-alkyl pendant; most importantly, some of these novel compounds exhibit features that might make them superior to the clinically approved PS Foscan.",
              "AIM: Head and neck squamous cell carcinoma (HNSCC) is one of the most prevalent types of cancer worldwide with unfavorable patient outcomes and relatively low survival rates. Long non-coding RNAs (lncRNAs) have been demonstrated to participate in the progression of HNSCC. The present study aimed to investigate the functional mechanism of lncRNA LINC00460 in HNSCC by mediating microRNA-206 (miR-206)/stanniocalcin-2 (STC2) axis. METHODS: The interactions among miR-206, LINC00460 and STC2 were identified, and the expression of LINC00460, miR-206 and STC2 in tissues and cells was determined. Gain- and loss-of function experiments were conducted to analyze effects of LINC00460, miR-206 and STC2 on the expression of apoptosis-related proteins, autophagy-related proteins, and the extents of AKT, ERK phosphorylation. Cell cycle distribution, apoptosis and the production of autophagosomes after transfection were evaluated to further explore the role of LINC00460/miR-206/STC2 axis in HNSCC. RESULTS: LINC00460 and STC2 were highly expressed while miR-206 was poorly expressed in HNSCC. Besides, miR-206 was found to bind to both LINC00460 and STC2. After the transfection of HNSCC cells with miR-206 mimic or si-LINC00460, the expression of STC2, AKT, ERK, as well as the extent of AKT, ERK phosphorylation all decreased, which facilitated the apoptosis and autophagy of HNSCC cells. CONCLUSION: Collectively, the apoptosis and autophagy of HNSCC can be facilitated by downregulating LINC00460, which highlights a novel target in the treatment of HNSCC.",
              "Drinking water with high levels of iodine has been identified as the key contributor to iodine excess, but the mechanisms of neurotoxicity induced by excessive iodine remain elusive. The present study aimed to explore the role of autophagy in the neurotoxic effect induced by excessive iodine in vivo. The Morris water maze test results demonstrated that excessive iodine impaired the learning and memory capabilities of rats, which were associated with marked body weight and brain weight abnormalities. In addition, iodine treatment increased malondialdehyde accumulation, decreased superoxide dismutase activity and glutathione (GSH) level, and enhanced levels of autophagy markers in the hippocampus. Notably, inhibition of autophagy with 3-methyladenine (3-MA) could significantly alleviate excessive iodine-induced cognitive impairment. These data imply that autophagy is involved in the cognitive impairment elicited by excessive iodine as a pathway of cell death, and inhibition of autophagy via 3-MA may significantly alleviate the above damage.",
              "OBJECTIVE: To investigate the effects of brazilin on the proliferation, apoptosis and autophagy of human tongue squamous cell carcinoma Tca8113 cells in vitro and explore its molecular mechanism. METHODS: The changes in the proliferation, morphology and apoptosis of Tca8113 cells in response to brazilin treatment were detected using MTT assay, Hoechst33342 staining, and Annexin V/PI double staining, respectively. The expressions of apoptosis-related protein Bax, Bcl-2, cleaved caspase-3 and autophagy-related proteins p-AMPK, p-mTOR, LC3B, and p62 in the treated cells were detected using Western blotting. The effect of treatment with both the AMPK pathway inhibitor and brazilin on the expressions of the pathway-related proteins p-AMPK, p-mTOR, and LC3B was assessed. RESULTS: MTT assay showed that brazilin significantly inhibited the proliferation of Tca8113 cells with an IC50 of 31.17 mumol/L at 24 h. Hoechst33342 staining showed that brazilin induced apoptotic morphological changes in Tca8113 cells in a concentration-dependent manner. Treatment with different concentrations of brazilin resulted in increased apoptosis in the cells. Brazilin obviously inhibited the expression of Bcl-2, p62 and p-mTOR and enhanced the expressions of Bax, cleaved caspase-3, LC3B and p-AMPK. The AMPK pathway inhibitor significantly inhibited the increase in p-AMPK and LC3B expressions and the decrease in p-mTOR expression induced by brazilin. CONCLUSIONS: Brazilin can inhibit the proliferation and promote apoptosis in Tca8113 cells and at the same time induces autophagy in the cells through the AMPK/mTOR pathway.",
              "OBJECTIVE: To explore the expression of the RNA-binding protein tristetraprolin in lung adenocarcinoma cells and its molecular mechanism for inhibiting autophagy. METHODS: Quantitative real-time PCR and Western blotting were performed to detect the expression of autophagy-related genes (including Beclin1, LC3-/LC3- and SQSTM1/p62) in cultured lung adenocarcinoma cells at 24, 48 and 72 h after transient transfection with a tristetraprolin-overexpressing plasmid and the empty plasmid. The effects of transfection with the tristetraprolin-overexpressing plasmid and empty plasmids in the presence or absence of tumor necrosis factor-alpha (TNF-alpha) on the expressions of nuclear factor-kappaB (NF-kappaB) p65, c-rel, and p50 were examined in lung adenocarcinoma cells using immunofluorescence assay and Western blotting. The cells were also transfected with the IkappaBalpha-mut plasmid and the tristetraprolin-overexpressing plasmid, either alone or in combination, and the changes in the expressions of tristetraprolin and autophagy-related genes were detected using RT-qPCR and Western blotting. RESULTS: The expressions of tristetraprolin were significantly reduced at both the mRNA and protein levels in lung adenocarcinoma cells (P &lt; 0.001). Overexpression of tristetraprolin in the cells significantly lowered the expressions of autophagy-related genes Beclin1 and the ratio of LC3-/LC3- at the mRNA and protein levels (P &lt; 0.001), obviously lowered the expressions of NF-kappaB p65 and c-rel, and almost totally blocked the nuclear translocation of NF-kappaB p65 and c-rel (P &lt; 0.05); the expression of p50, however, did not undergo significant changes in response to tristetraprolin overexpression (P &gt; 0.05). The inhibitory effect of tristetraprolin overexpression on autophagy was abrogated by transfection of the cells with IkappaBalpha-mut plasmid, which blocked the NF-kappaB signaling pathway. Co-transfection of the cells with IkappaBalpha-mut also attenuated the inhibitory effect of tristetraprolin overexpression on Beclin1 and the LC3-/LC3- ratio at both the mRNA and protein levels (P &lt; 0.05). CONCLUSIONS: The expression of tristetraprolin is low in lung adenocarcinoma cells. Tristetraprolin overexpression causes inhibition of autophagy in lung adenocarcinoma cells possibly by blocking NF-kappaB p65 and c-rel nuclear translocation.",
              "OBJECTIVE: To explore the molecular mechanism of sorafenib resistance in hepatoma cells and identify for new targets to reverse drug resistance. METHODS: THP-1 cells were induced into M2 tumor-associated macrophages (M2-TAMs) in vitro and identified by immunofluorescence. SMMC-7721 cells were co-cultured with M2-TAMs with or without sorafenib treatment. CCK-8 assay was used to observe the inhibitory effect of sorafenib on the cell proliferation. Annexin V/PI double staining and protein immunoblotting were used to assess the effect of sorafenib on the proliferation, apoptosis and the expressions of apoptosis-related proteins and autophagy-related protein in SMMC-7721 cells co-cultured with M2-TAMs in the presence or absence of the autophagy inhibitor chloroquine (CQ). RESULTS: The IC50 of sorafenib at 48 h was 2.25 mumol/L in SMMC-7721 cells cultured alone, and increased to 4.72 mumol/L in the cells co-cultured with M2-TAMs. Compared with the cells cultured alone, the co-cultured SMMC-7721 cells showed significantly reduced apoptosis rate in response to sorafenib (P &lt; 0.01) and significantly increased expression of Bcl-2 and Bcl-2/Bax ratio (P &lt; 0.05) with also increased LC3-II/LC3-I ratio (P &lt; 0.001) and lowered expression of p62 (P &lt; 0.05), suggesting a significantly enhanced level of autophagy. CQ treatment significantly inhibited the proliferation of the co-cultured SMMC-7721 cells (P &lt; 0.05), increased the cell apoptosis (P &lt; 0.05) and reduced the Bcl-2/Bax ratio (P &lt; 0.01). CONCLUSIONS: M2-TAMs can attenuate the inhibitory effect of sorafenib on the proliferation of hepatoma cells by increasing the level of autophagy, suggesting a new strategy for reversing sorafenib resistance induced by the tumor microenvironment by inhibiting autophagy.",
              "Oxygen deprivation affects human health by modulating system as well as cellular physiology. Hypoxia generates reactive oxygen species (ROS), causes oxidative stress and affects female reproductive health by altering ovarian as well as oocyte physiology in mammals. Hypoxic conditions lead to several degenerative changes by inducing various cell death pathways like autophagy, apoptosis and necrosis in the follicle of mammalian ovary. The encircling somatic cell death interrupts supply of nutrients to the oocyte and nutrient deprivation may result in the generation of ROS. Increased level of ROS could induce granulosa cells as well as oocyte autophagy. Although autophagy removes damaged proteins and subcellular organelles to maintain the cell survival, irreparable damages could induce cell death within intra-follicular microenvironment. Hypoxia-induced autophagy is operated through 5' AMP activated protein kinase-mammalian target of rapamycin, endoplasmic reticulum stress/unfolded protein response and protein kinase C delta-c-junN terminal kinase 1 pathways in a wide variety of somatic cell types. Similar to somatic cells, we propose that hypoxia may induce granulosa cell as well as oocyte autophagy and it could be responsible at least in part for germ cell elimination from mammalian ovary. Hypoxia-mediated germ cell depletion may cause several reproductive impairments including early menopause in mammals.",
              "Renal cancer is one of the most common malignant urological tumors; however, its diagnosis and treatment are not well established. In the present study, we identified that CDK5 regulatory subunit-associated protein 3 (CDK5RAP3), a putative tumor suppressor in many cancers, was downregulated in renal cancer tissues. Through loss- and gain-of-function experiments, we observed that the action of CDK5RAP3 in renal cancer cells was different in Caki-1 and 769-P cell lines. Knockdown of endogenous CDK5RAP3 in Caki-1 slightly increased cell viability, whereas overexpression of CDK5RAP3 in 769-P cells inhibited cell viability. In addition, we observed that CDK5RAP3 participated in the regulation of autophagy in renal cancer. Knockdown of CDK5RAP3 induced significant inhibition of autophagy in Caki-1 cells but not in 769-P cells. In contrast, overexpression of CDK5RAP3 significantly activated autophagy in 769-P cells, as evidenced by increased LC3-II levels. However, the LC3-II could not be altered by CDK5RAP3 overexpression in Caki-1 cells. These findings demonstrated that CDK5RAP3 is downregulated in renal cancer and may be associated with autophagy.",
              "Chondroitin sulfate (CS) and heparan sulfate (HS) are glycosaminoglycans that both bind the receptor-type protein tyrosine phosphatase PTPRsigma, affecting axonal regeneration. CS inhibits axonal growth, while HS promotes it. Here, we have prepared a library of HS octasaccharides and, together with synthetic CS oligomers, we found that PTPRsigma preferentially interacts with CS-E-a rare sulfation pattern in natural CS-and most HS oligomers bearing sulfate and sulfamate groups. Consequently, short and long stretches of natural CS and HS, respectively, bind to PTPRsigma. CS activates PTPRsigma, which dephosphorylates cortactin-herein identified as a new PTPRsigma substrate-and disrupts autophagy flux at the autophagosome-lysosome fusion step. Such disruption is required and sufficient for dystrophic endball formation and inhibition of axonal regeneration. Therefore, sulfation patterns determine the length of the glycosaminoglycan segment that bind to PTPRsigma and define the fate of axonal regeneration through a mechanism involving PTPRsigma, cortactin and autophagy.",
              "In solid tumors, cancer cells are exposed to microenvironment stress, including hypoxia and insufficient nutrients. An acidic microenvironment in tumors is facilitated by the increase in synthesis of lactic acid; this is known as Warburg effect. We previously showed that B16F10 melanoma cells were induced autophagic cell death by glucose-deprivation stress, and lactic acid suppressed the cell death through the inhibition of autophagy. In this study, effects of lactic acid on cell death of B16F10 cells under hypoxic and glucose-depleted double stress conditions were investigated. The double stress promoted autophagic cell death earlier than glucose-depleted stress alone. Lactic acid repressed the double stress-induced cell death by inhibiting autophagy. These results suggest that lactic acid serves for cell survival under microenvironmental stress conditions in B16F10 melanoma cells.",
              "The mammalian target of rapamycin(mTOR)is a serine/threonine protein kinase that regulates protein synthesis and degradation,cytoskeletal formation,and cell longevity.Autophagy,a catabolic process necessary for the maintenance of intracellular homeostasis,is essential for cell survival,whereas mTOR is the crucial regulator of autophagy.Alzheimer's disease(AD)is the most common cause of progressive dementia in the elderly.It has been shown that disorders of mTOR and autophagy signaling pathways are closely related to AD.In the present review,we describe the regulatory roles of mTOR signaling and autophagy pathway in AD brain and introduce drugs for AD acting via modulation of autophagy and mTOR.",
              "In recent years, functional interconnections emerged between synaptic transmission, inflammatory/immune mediators, and central nervous system (CNS) (patho)-physiology. Such interconnections rose up to a level that involves synaptic plasticity, both concerning its molecular mechanisms and the clinical outcomes related to its behavioral abnormalities. Within this context, synaptic plasticity, apart from being modulated by classic CNS molecules, is strongly affected by the immune system, and vice versa. This is not surprising, given the common molecular pathways that operate at the cross-road between the CNS and immune system. When searching for a common pathway bridging neuro-immune and synaptic dysregulations, the two major cell-clearing cell clearing systems, namely the ubiquitin proteasome system (UPS) and autophagy, take center stage. In fact, just like is happening for the turnover of key proteins involved in neurotransmitter release, antigen processing within both peripheral and CNS-resident antigen presenting cells is carried out by UPS and autophagy. Recent evidence unravelling the functional cross-talk between the cell-clearing pathways challenged the traditional concept of autophagy and UPS as independent systems. In fact, autophagy and UPS are simultaneously affected in a variety of CNS disorders where synaptic and inflammatory/immune alterations concur. In this review, we discuss the role of autophagy and UPS in bridging synaptic plasticity with neuro-immunity, while posing a special emphasis on their interactions, which may be key to defining the role of immunity in synaptic plasticity in health and disease.",
              "Methamphetamine (METH) is a widely abused illicit psychoactive drug. Our previous study has shown that CCAAT-enhancer binding protein beta (C/EBPbeta) is an important regulator in METH-induced neuronal autophagy and apoptosis. However, the detailed molecular mechanisms underlying this process remain poorly understood. Previous studies have demonstrated that DNA damage-inducible transcript 4 (DDIT4), Trib3 (tribbles pseudo kinase 3), alpha-synuclein (alpha-syn) are involved in METH-induced dopaminergic neurotoxicity. We hypothesized that C/EBPbeta is involved in METH-induced DDIT4-mediated neuronal autophagy and Trib3-mediated neuronal apoptosis. We tested our hypothesis by examining the effects of silencing C/EBPbeta, DDIT4, Trib3 or alpha-syn with small interfering ribonucleic acid (siRNA) on METH-induced autophagy and apoptosis in the human neuroblastoma SH-SY5Y cells. We also measured the levels of phosphorylated tuberous sclerosis complex 2 (TSC2) protein and Parkin protein level in SH-SY5Y cells. Furthermore, we demonstrated the effect of silencing C/EBPbeta on METH-caused neurotoxicity in the striatum of rats by injecting LV-shC/EBPbeta lentivirus using a stereotaxic positioning system. The results showed that METH exposure increased C/EBPbeta, DDIT4 protein expression. Elevated DDIT4 expression raised up p-TSC2/TSC2 protein expression ratio, inhibited mTOR signaling pathway, activating cell autophagy. We also found that METH exposure increased the expression of Trib3, alpha-syn, decreased the Parkin protein expression. Lowering levels of Parkin raised up alpha-syn expression, which initiated mitochondrial apoptosis by down-regulating anti-apoptotic Bcl-2, followed by up-regulation of pro-apoptotic Bax, resulting in translocation of cytochrome c (cyto c), an apoptogenic factor, from the mitochondria to cytoplasm and activation of caspase-dependent pathways. These findings were supported by data showing METH-induced autophagy and apoptosis was significantly inhibited by silencing C/EBPbeta, DDIT4, Trib3 or alpha-syn, or by Parkin over-expression. Based on the present data, a novel of mechanism on METH-induced cell toxicity is proposed, METH exposure increased C/EBPbeta protein expression, triggered DDIT4/TSC2/mTOR signaling pathway, and evoked Trib3/Parkin/alpha-syn-related mitochondrial apoptotic signaling pathway. Collectively, these results suggest that C/EBPbeta plays an important role in METH-triggered autophagy and apoptosis and it may be a potential target for therapeutics in METH-caused neurotoxicity.",
              "Polyether compounds, a large group of biologically active metabolites produced by Streptomyces species have been reported to show a variety of bioactivity such as antibacterial, antifungal, antiparasitic, antiviral, and tumour cell cytotoxicity. Since some of these compounds target cancer stem cells and multi-drug resistant cancer cells, this family of compounds have become of high interest. In this study, three polyether-type metabolites (1-3), one of which was a new natural product (3), were isolated from the marine derived Streptomyces cacaoi via antimicrobial activity-guided fractionation studies. As several polyether compounds with structural similarity such as monensin have been linked with autophagy and cell death, we first assessed the cytotoxicity of these three compounds. Compounds 2 and 3, but not 1, were found to be cytotoxic in several cell lines with a higher potency towards cancer cells. Furthermore, 2 and 3 caused accumulation of both autophagy flux markers LC3-II and p62 along with cleavage of caspase-3, caspase-9 and poly (ADP-ribose) polymerase 1 (PARP-1). Interestingly, prolonged treatment of the compounds caused a dramatic downregulation of the proteins related to autophagasome formation in a dose dependent manner. Our findings provide insights on the molecular mechanisms of the polyether-type polyketides, and signify their potency as chemotherapeutic agents through inhibiting autophagy and inducing apoptosis.",
              "To assess the effect of cluster of differentiation (CD47) downregulation on autophagy in hypoxia/reoxygenation (H/R)treated H9c2 cardiomyocytes. H9c2 cells were maintained in normoxic conditions (95% air, 5% CO2, 37 C) without CD47 antibodies, SiCD47 or chloroquine (CQ) treatment; H9c2 cells in the H/R group were subjected to 24 h of hypoxia (1% O2, 94% N2, 5% CO2, 37 C) followed by 12 h of reoxygenation (95% air, 5% CO2, 37 C). All assays were controlled, triplicated and repeated on three separately initiated cultures. The biochemical parameters in the medium supernatant were measured to evaluate the oxidative stress in cardiomyocytes. The Annexin Vfluorescein isothiocyanate assay was used to detect the apoptotic rate in the H9c2 cells. Transmission electron microscope, immunofluorescent staining and western blot analysis were performed to detect the effect of the CD47 antibody on autophagic flux in H/Rtreated H9c2 cardiomyocytes. The cardiomyocytic oxidative stress and apoptotic rate decreased and autophagic clearance increased after CD47 downregulation. H/R triggered cell autophagy, autophagosome accumulation and apoptosis in H9c2 cell lines. However, these effects can be attenuated by CD47 downregulation. This study demonstrates its clinical implications in ischemia/reperfusion injury treatment.",
              "Interleukin 17A (IL17A) exerts pleiotropic effects on periodontitis, partially through enhancement of alveolar bone loss. Osteoclasts are the main culprits that absorb alveolar bone. However, studies describing the correlation between IL17A and osteoclasts are not conclusive. Previously, autophagy was revealed to be involved in osteoclast differentiation and bone resorption. However, the role of autophagy in IL17Amediated osteoclast formation is yet to be clarified. In the present study, bone marrow macrophages (BMMs) were treated with or without IL17A. 3Methyladenine (3MA) was applied to inhibit autophagy. Osteoclast formation was detected by tartrateresistant acid phosphatase (TRAP) staining, immunofluorescence, and scanning electron microscope. The effects of IL17A on osteoclastspecific genes and autophagyrelated genes during osteoclast differentiation were examined by realtime quantitative polymerase chain reaction and western blot analysis. Autophagosomes were observed by transmission electron microscope. Hematoxylin and eosin (H&E), and TRAP staining was adopted to assess alveolar bone destruction and the number of osteoclasts, respectively in a rat periodontitis model. Consequently, IL17A stimulated osteoclast differentiation and bone resorption of BMMs accompanied by an increase in the mRNA expression of osteoclastspecific genes. Furthermore, IL17A increased the levels of autophagyrelated genes and proteins, and inhibition of autophagy with 3MA attenuated the IL17Amediated osteoclastogenesis. In addition, there was an increase in the number of osteoclasts and alveolar bone resorption with IL17A treatment in the periodontitis rat model. Collectively, these findings indicated that IL17A facilitated osteoclast differentiation and bone resorption in vitro and in vivo, which may contribute to the understanding of the molecular basis of IL17A in alveolar bone destruction and provide insight on the clinical therapeutic targets for periodontitis.",
              "Inflammation has been implicated in the pathogenesis of type 2 diabetes (T2D), which is a progressive disease characterized by pancreatic betacell dysfunction and apoptosis with consequential insufficient insulin secretion. Autophagy is necessary to maintain the structure, mass and function of pancreatic betacells. The present study investigated the crosstalk between autophagy and inflammasome activation in T2D. INS1 cells were stimulated with lipopolysaccharide. Apoptosis and reactive oxygen species (ROS) formation were measured using flow cytometry, and cell proliferation was measured using Cell Counting Kit8 solution. Autophagy was assayed using western blotting and transmission electron microscopy. The expression levels of interleukin1beta (IL1beta) and caspase1 were detected by western blotting. The results demonstrated that inhibiting autophagy using 3methyladenine (3MA) promoted INS1 cell apoptosis. This response was correlated with an increase in ROS production and the inflammatory response, including IL1beta maturation and caspase1 activation. Furthermore, when ROS were inhibited using NacetylLcysteine, inflammation was decreased. These results demonstrated that inhibition of autophagy enhanced inflammatory injury via the ROSmediated activation of the Nodlike receptor pyrin domaincontaining protein 3 inflammasome. Autophagy may have a protective effect by mitigating inflammation in T2D, which may provide a novel approach for T2D treatment.",
              "Zinc is an essential trace element and plays critical roles in cellular integrity and biological functions. Excess copper induced both oxidative stress and endoplasmic reticulum (ER) stress in liver-derived cultured cells. Excess copper also induced impairment of autophagic flux at the step of autophagosome-lysosome fusion, as well as Mallory-Denk body (MDB)-like inclusion body formation. Zinc ameliorated excess copper-induced impairment of autophagic flux and MDB-like inclusion body formation via the maintenance of ER homeostasis. Furthermore, zinc also ameliorated free fatty acid-induced impairment of autophagic flux. These results indicate that zinc may be able to protect hepatocytes from various ER stress-related conditions.",
              "The mitochondrion is an essential organelle important for the generation of ATP for cellular function. This is especially critical for cells with high energy demands, such as neurons for signal transmission and cardiomyocytes for the continuous mechanical work of the heart. However, deleterious reactive oxygen species are generated as a result of mitochondrial electron transport, requiring a rigorous activation of antioxidative defense in order to maintain homeostatic mitochondrial function. Indeed, recent studies have demonstrated that the dysregulation of antioxidant response leads to mitochondrial dysfunction in human degenerative diseases affecting the nervous system and the heart. In this review, we outline and discuss the mitochondrial and oxidative stress factors causing degenerative diseases, such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, and Friedreich's ataxia. In particular, the pathological involvement of mitochondrial dysfunction in relation to oxidative stress, energy metabolism, mitochondrial dynamics, and cell death will be explored. Understanding the pathology and the development of these diseases has highlighted novel regulators in the homeostatic maintenance of mitochondria. Importantly, this offers potential therapeutic targets in the development of future treatments for these degenerative diseases.",
              "BACKGROUND: Colorectal cancer (CRC) is one of the main causes of cancer-related deaths in China and around the world. Advanced CRC (ACRC) patients suffer from a low cure rate though treated with targeted therapies. The response rate is about 50% to chemotherapy and cetuximab, a monoclonal antibody targeting epidermal growth factor receptor (EGFR) and used for ACRC with wild-type KRAS. It is important to identify more predictors of cetuximab efficacy to further improve precise treatment. Autophagy, showing a key role in the cancer progression, is influenced by the EGFR pathway. Whether autophagy can predict cetuximab efficacy in ACRC is an interesting topic. AIM: To investigate the effect of autophagy on the efficacy of cetuximab in colon cancer cells and ACRC patients with wild-type KRAS. METHODS: ACRC patients treated with cetuximab plus chemotherapy, with detailed data and tumor tissue, at Sun Yat-sen University Cancer Center from January 1, 2005, to October 1, 2015, were studied. Expression of autophagy-related proteins [Beclin1, microtubule-associated protein 1A/B-light chain 3 (LC3), and 4E-binding protein 1 (4E-BP1)] was examined by Western blot in CRC cells and by immunohistochemistry in cancerous and normal tissues. The effect of autophagy on cetuximab-treated cancer cells was confirmed by MTT assay. The associations between Beclin1, LC3, and 4E-BP1 expression in tumor tissue and the efficacy of cetuximab-based therapy were analyzed. RESULTS: In CACO-2 cells exposed to cetuximab, LC3 and 4E-BP1 were upregulated, and P62 was downregulated. Autophagosome formation was observed, and autophagy increased the efficacy of cetuximab. In 68 ACRC patients, immunohistochemistry showed that Beclin1 levels were significantly correlated with those of LC3 (0.657, P < 0.001) and 4E-BP1 (0.211, P = 0.042) in ACRC tissues. LC3 was significantly overexpressed in tumor tissues compared to normal tissues (P < 0.001). In 45 patients with wild-type KRAS, the expression levels of these three proteins were not related to progression-free survival; however, the expression levels of Beclin1 (P = 0.010) and 4E-BP1 (P = 0.005), pathological grade (P = 0.002), and T stage (P = 0.004) were independent prognostic factors for overall survival (OS). CONCLUSION: The effect of cetuximab on colon cancer cells might be improved by autophagy. LC3 is overexpressed in tumor tissues, and Beclin1 and 4E-BP1 could be significant predictors of OS in ACRC patients treated with cetuximab.",
              "OBJECTIVE: To observe the effect of moxibustion of acupoints of the Governor Vessel on the levels of cellular autophagy, beta amyloid protein (Abeta) immunoactivity, and expression of LC3- LC3- p62 and p-P70S6K proteins in the hippocampal tissue of APPswe/PS1de9 (APP/PS1) double-transgenic Alzheimer's disease (AD) mice, so as to reveal its underlying mechanisms in improving AD. METHODS: APP/PS1 double-transgenic AD mice were randomly divided into AD model, moxibustion, autophagy-inducer (Rapamycin) and autophagy-inhibitor (3-MA)moxibustion groups (n10 in each group), and other 10 C57BL/6J male mice (the same age) were used as the normal control group. Herbal-cake (made of Chuanwu [Radix Aconiti Praeparata]) partitioned moxibustion was applied to \"Baihui\"(GV20), moxibustion was applied to \"Fengfu\"(GV16) and \"Dazhui\"(GV14), all for 20 min, once daily for 2 weeks, with one day's off between two weeks. For mice of the autophagy-inducer and 3-MAmoxibustion groups, Rapamycin (2 mg*kg(1)*d(1)) and 3-MA (1.5 mg*kg(1)*d(1)) were separately administered by intraperitoneal injection for 2 weeks. The cognitive ability was examined by Morris water maze tests, and the ultrastructural changes (including autophagic lysosomes, etc.) of hippocampal neurons were observed by using transmission electron microscopy. The immunoactivity of cerebral cortex and hippocampal Amyloid beta peptide 1-42 (Abeta1-42) was detected by immunohistochemistry, and the expression levels of hippocampal LC3- LC3- p62 and p-P70S6K proteins were detected by Western blot. RESULTS: After modeling, the escape latency of Morris water maze tasks was prolonged in the model group than in the normal control group (P<0.05) and obviously shortened in the moxibustion and autophagy-inducer groups (not the autophagy-inhibitor group) than in the model group (P<0.05). Results of transmission electron microscope showed deformed, irregular or atrophic neurons with rough and incomplete and fuzzy nuclear membrane, and decreased intracellular autophagosomes in the hippocampus in the model group, and partial irregular, atrophic neurons with more autophagic vesicles and lysosomes in the moxibustion group. The expression levels of Abeta1-42 in both cerebral cortex and hippocampus tissues, and LC3- p62 and p-P70S6K proteins in the hippocampus were consi-derably up-regulated in the model group relevant to the normal control group (P<0.01), and evidently down-regulated in both moxibustion and autophagy-inducer groups (not the autophagy-inhibitor group) than in the model group (P<0.01), while that of hippocampal LC3- protein and LC3-/ ratio levels were obviously down-regulated in the model group relevant to the normal control group (P<0.01), and significantly up-regulated in both moxibustion and autophagy-inducer groups (not the autophagy-inhibitor group) than in the model group (P<0.01). CONCLUSION: Moxibustion can improve the cognitive ability of APP/PS1 double-transgenic AD mice, which is associated with its effects in promoting hip-pocampal and cerebral cortex autophagy level, and down-regulating the expression levels of Abeta1-42, LC3- p62 and p-P70S6K proteins in the hippocampus.",
              "Excessive accumulation of cadmium (Cd) in retina plays an important role in tobacco smoking-associated age-related macular degeneration (AMD). Plenty of evidence has revealed that the retinal pigment epithelium (RPE) is the primary site of pathology in AMD. Our current study demonstrated that Cd induced apoptosis in a human RPE cell line ARPE-19 cells, as it dose-dependently caused cell viability loss and activated caspase-3. The reactive oxygen species (ROS) were confirmed to be important mediators for Cd-triggered cell death in ARPE-19 cells. We found that endoplasmic reticulum (ER) stress was activated as its marker BiP was remarkably upregulated by Cd-exposure. Whereas the antioxidants N-acetylcysteine (NAC) and Tempol significantly suppressed the expression of BiP and CHOP, suggesting that ROS generation is an early trigger of Cd-activated ER stress. Furthermore, we found that Cd-induced oxidative stress significantly increased autophagic flux and p62 expression. A temporal impact of Cd exposure is possibly existed in p62 expression in ARPE-19 cells. Moreover, an ER stress inhibitor salubrinal diminished Cd-induced LC3BII expression and attenuated cytotoxicity, indicating that ER stress mediates autophagy and was implicated in apoptosis of Cd-exposed ARPE-19 cells. However, CHOP expression may not exert impact on the regulation of Cd-caused autophagy. Additionally, inhibition of autophagy with si-Beclin 1 and 3-Methyladenine significantly ameliorated Cd-induced CHOP expression and cytotoxicity, indicating that autophagy was detrimental in Cd-accumulated ARPE-19 cells, and a positive feedback regulation mechanism may exist between Cd-triggered ER stress and autophagy. Taken together, these results suggest that Cd-caused ER stress and autophagy are implicated in RPE cell death associated retinopathies especially related to smoking.",
              "The macrophage innate immune response is outlined through recognition of the components of Mycobacterium tuberculosis. DNA of M. tuberculosis (MtbDNA) is recognized by macrophages, but the implications of this recognition are poorly characterized. Stimulation of murine macrophages with MtbDNA induces autophagy, a process that promotes elimination of intracellular pathogens. However, it remains unknown whether this or other phenomena also occur in human cells. In this work, we studied the innate response profiles of human macrophages after stimulation with DNA from virulent M. tuberculosis H37Rv. Human monocyte-derived macrophages were polarized into M1 and M2 phenotypes and stimulated with MtbDNA. The plasma membrane markers of the phenotype, production of TNF-alpha, and induction of autophagy were evaluated. Our results indicate that MtbDNA induced phenotypical changes, the significant production of TNF-alpha, and autophagy confirmed by the augmented expression of immunity related GTPase M (IRGM) and autophagy related ATG16L1 genes in M1 macrophages, whereas M2 macrophages exhibited limited responses. In addition, MtbDNA activation was TLR-9-dependent. Although TLR-9 expression was similar between M1 and M2 macrophages, only M1 macrophages were fully responsive to MtbDNA. In conclusion, MtbDNA recognition enhanced the antimicrobial mechanisms of M1 macrophages.",
              "Arginase I has been documented to impair T cell function and attenuate cellular immunity, however, there is little evidence to reveal the effect of arginase I on macrophage function. Recently, recombinant human arginase I (rhArg) has been developed for cancer therapy and is in clinical trial for hepatocellular carcinoma, whereas the potential immunosuppression induced by rhArg limited its therapeutic efficacy. To improve the clinical outcome of rhArg, addressing the immune suppression appears to be particularly important. In this study, we found that rhArg attenuated macrophage functions, including inhibiting macrophage cell proliferation, nitric oxide (NO) and reactive oxygen species (ROS) production, cytokine secretion, MHC-II surface expression, and phagocytosis, thereby inducing immunosuppression in lipopolysaccharides (LPS)/interferon-gamma (IFN-gamma)-activated macrophages. Notably, we observed that rhArg downregulated autophagy in activated macrophages. Moreover, application of trehalose (an autophagy inducer) significantly restored the impaired immune function in activated macrophages, suggesting the essential role of autophagy in rhArg-induced immunosuppression. To further illustrate the effect of autophagy in immunosuppression, we then observed the effect of 3-MA (an autophagy inhibitor) on the immune function of macrophages. As expected, inhibiting autophagy by 3-MA attenuated immune functions in activated macrophages. Collectively, this study elucidated that rhArg induced immunosuppression in activated macrophages via inhibiting autophagy, providing potential strategy to ameliorate the immune suppression which is of great significance to cancer therapy and facilitating the development of rhArg as a potential therapy for malignant carcinomas.",
              "Mitophagy is the selective autophagic targeting and removal of dysfunctional mitochondria. While PINK1/Parkin-dependent mitophagy is well-characterized, PINK1/Parkin-independent route is poorly understood. Using structure illumination microscopy (SR-SIM), we demonstrate that the SNARE protein Syntaxin 17 (STX17) initiates mitophagy upon depletion of outer mitochondrial membrane protein Fis1. With proteomics analysis, we identify the STX17-Fis1 interaction, which controls the dynamic shuffling of STX17 between ER and mitochondria. Fis1 loss results in aberrant STX17 accumulation on mitochondria, which exposes the N terminus and promotes self-oligomerization to trigger mitophagy. Mitochondrial STX17 interacts with ATG14 and recruits core autophagy proteins to form mitophagosome, followed by Rab7-dependent mitophagosome-lysosome fusion. Furthermore, Fis1 loss impairs mitochondrial respiration and potentially sensitizes cells to mitochondrial clearance, which is mediated through canonical autophagy machinery, closely linking non-selective macroautophagy to mitochondrial turnover. Our findings uncover a PINK1/Parkin-independent mitophagic mechanism in which outer mitochondrial membrane protein Fis1 regulates mitochondrial quality control.",
              "Autophagy is an essential recycling and quality control pathway. Mammalian ATG8 proteins drive autophagosome formation and selective removal of protein aggregates and organelles by recruiting autophagy receptors and adaptors that contain a LC3-interacting region (LIR) motif. LIR motifs can be highly selective for ATG8 subfamily proteins (LC3s/GABARAPs), however the molecular determinants regulating these selective interactions remain elusive. Here we show that residues within the core LIR motif and adjacent C-terminal region as well as ATG8 subfamily-specific residues in the LIR docking site are critical for binding of receptors and adaptors to GABARAPs. Moreover, rendering GABARAP more LC3B-like impairs autophagy receptor degradation. Modulating LIR binding specificity of the centriolar satellite protein PCM1, implicated in autophagy and centrosomal function, alters its dynamics in cells. Our data provides new mechanistic insight into how selective binding of LIR motifs to GABARAPs is achieved, and elucidate the overlapping and distinct functions of ATG8 subfamily proteins.",
              "BACKGROUND: Celastrol, a triterpene compound derived from the traditional Chinese medicine Tripterygium wilfordii, has been reported to possess potential antitumor activity towards various malignancies. However, the effect of celastrol on glioma cells and the underlying molecular mechanisms remain elusive. METHODS: Glioma cells, including the U251, U87-MG and C6 cell lines and an animal model were used. The effects of celastrol on cells were evaluated by flow cytometry, confocal microscopy, reactive oxygen species production assay and immunoblotting after treatment of celastrol. Fisher's exact test, a one-way ANOVA and the Mann-Whitney U-test were used to compare differences between groups. All data were analyzed using SPSS version 21.0 software. RESULTS: Here, we found that exposure to celastrol induced G2/M phase arrest and apoptosis. Celastrol increased the formation of autophagosomes, accumulation of LC3B and the expression of p62 protein. Celastrol-treated glioma cells exhibited decreased cell viability after the use of autophagy inhibitors. Additionally, autophagy and apoptosis caused by celastrol in glioma cells inhibited each other. Furthermore, celastrol induced JNK activation and ROS production and inhibited the activities of Akt and mTOR kinases. JNK and ROS inhibitors significantly attenuated celastrol-trigged apoptosis and autophagy, while Akt and mTOR inhibitors had opposite effects. CONCLUSIONS: In conclusion, our study revealed that celastrol caused G2/M phase arrest and trigged apoptosis and autophagy by activating ROS/JNK signaling and blocking the Akt/mTOR signaling pathway.",
              "BACKGROUND: Malignant peripheral nerve sheath tumours (MPNSTs) are sarcomas of Schwann cell lineage origin that occur sporadically or in association with the inherited syndrome, neurofibromatosis type 1 (NF1). This study aimed to examine the function of High mobility group protein A2 (HMGA2) in NF1 MPNST progression and the underlying molecular mechanism. METHODS: Immunohistochemistry (IHC) was used to detect HMGA2 expression in MPNST and neurofibroma patient samples. Cell Cycle Kit-8 (CCK-8) and 5-ethynyl-20-deoxyuridine (EdU) assays, terminal deoxynucleotidyl transferase-mediated nick end labelling, and transmission electron microscopy were performed to reveal HMGA2 functions in NF1 MPNST cells in vitro and in vivo. Chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA sequencing (RNA-Seq) were used to detect HMGA2-modulated genes regulating autophagy and growth in NF1 MPNSTs in vitro and in vivo. RESULTS: NF1 MPNST samples exhibit higher HMGA2 positivity rates (13/16) than sporadic MPNST (16/41) and neurofibroma (0/7) patient samples. High HMGA2 expression is correlated with poor prognosis. Neurofibromin 1 (NF1)-deficient MPNST cells display elevated HMGA2 expression. Functional experiments revealed that HMGA2 knockdown inhibits NF1 MPNST cell growth in vitro and in vivo. In addition to promoting cell cycle arrest and apoptosis, HMGA2 knockdown inhibits autophagy, favouring cell death. RNA-Seq and ChIP-Seq revealed that HMGA2 directly activates the Musashi-2 (MSI2) promoter region, and MSI2 overexpression reverses autophagy and growth in shHMGA2-transfected cells. MSI2 interacts with Beclin1, and Beclin1 blockade inhibits autophagy, thereby inhibiting cell proliferation. CONCLUSIONS: HMGA2 knockdown regulates autophagy via MSI2-Beclin1 interactions to inhibit NF1 MPNST growth, revealing potential therapeutic targets for these untreatable tumours.",
              "BACKGROUND: Acquired resistance to sorafenib greatly limits its therapeutic efficiency in the treatment of hepatocellular carcinoma (HCC). Increasing evidence indicates that long noncoding RNAs (lncRNAs) play important roles in the resistance to anti-cancer drugs. The present study aims to explore the involvement of lncRNA SNHG1 (small nucleolar RNA host gene 1) in sorafenib resistance and how SNHG1 is associated with overexpressed microRNA-21 (miR-21) and the activated Akt pathway, which have been demonstrated to mediate this resistance in HCC cells. METHODS: Sorafenib-resistant HCC (SR-HCC) cells were generated and their sorafenib-resistant properties were confirmed by cell viability and apoptosis assays. Potential lncRNAs were screened by using multiple bioinformatics analyses and databases. The expression of genes and proteins was detected by qRT-PCR, Western blot and in situ hybridization. Gene silencing was achieved by specific siRNA or lncRNA Smart Silencer. The effects of anti-SNHG1 were evaluated in vitro and in experimental animals by using quantitative measures of cell proliferation, apoptosis and autophagy. The binding sites of miR-21 and SNHG1 were predicted by using the RNAhybrid algorithm and their interaction was verified by luciferase assays. RESULTS: The Akt pathway was highly activated by overexpressed miR-21 in SR-HCC cells compared with parental HCC cells. Among ten screened candidates, SNHG1 showed the largest folds of alteration between SR-HCC and parental cells and between vehicle- and sorafenib-treated cells. Overexpressed SNHG1 contributes to sorafenib resistance by activating the Akt pathway via regulating SLC3A2. Depletion of SNHG1 enhanced the efficacy of sorafenib to induce apoptosis and autophagy of SR-HCC cells by inhibiting the activation of Akt pathway. Sorafenib induced translocation of miR-21 to the nucleus, where it promoted the expression of SNHG1, resulting in upregulation of SLC3A2, leading to the activation of Akt pathway. In contrast, SNHG1 was shown to have little effect on the expression of miR-21, which downregulated the expression of PTEN, leading to the activation of the Akt pathway independently of SNHG1. CONCLUSIONS: The present study has demonstrated that lncRNA SNHG1 contributes to sorafenib resistance by activating the Akt pathway and its nuclear expression is promoted by miR-21, whose nuclear translocation is induced by sorafenib. These results indicate that SNHG1 may represent a potentially valuable target for overcoming sorafenib resistance for HCC.",
              "AIMS: Cigarette smoking results in well-known negative reproductive consequences. However, the role of histone deacetylase 1 and 2 (HDAC1/2) in the structural changes of uterine tissues induced by cigarette smoke (CS) exposure and the therapeutic potential of trichostatin A (TSA), a HDAC inhibitor, have not been investigated. MAIN METHODS: Female mice were exposed to CS twice daily for 30days and TSA was injected intraperitoneally into CS-exposed mice on alternate days in the TSA-treated group. Uteri in the estrus phase were weighed and uterine histomorphology and HDAC1 cell distribution were examined by HE and immunohistochemistry. Markers associated with macro-autophagy (Beclin-1), autophagic flux (increased LC3-II and a lack of p62 accumulation), autophagy inhibiting factor (mTOR, phosphorylated mTOR and its upstream IRS, phosphorylated IRS), HDAC1/2, FOXO1 and FOXO3 were assessed by Western blot. KEY FINDINGS: CS exposure decreased body weight and triggered uterine histomorphologic alterations, including a thinner myometrium and a reduced number of glandular and interstitial cells. HDAC1/2 were activated in uterine tissues after CS exposure and TSA effectively inhibited HDAC1/2 activation and attenuated the loss of body weight and uterine wet weight induced by CS exposure. TSA effectively restored the thickness of the myometrium and number of glandular and interstitial cells. TSA also restored the expression of markers of macro-autophagy (LC3-II and Beclin-1) and reduced phosphorylated mTOR, phosphorylated IRS, FOXO1 and FOXO3 activation. SIGNIFICANCE: TSA inhibited uterine histomorphologic alterations induced by CS exposure. The TSA effect might be associated with resumption of macro-autophagy via HDAC1/2 inhibition.",
              "BACKGROUND/AIMS: Atherosclerosis is a chronic inflammatory cardiovascular disease. Macrophages are major components of atherosclerotic plaques and play a key role in the development of atherosclerosis by secreting a variety of pro-inflammatory factors. Our previous studies have confirmed that upconversion nanoparticles encapsulating chlorin e6 (UCNPs-Ce6) mediated photodynamic therapy (PDT) can promote cholesterol efflux and induce apoptosis in THP-1 macrophages. In this study, we investigated whether reactive oxygen species (ROS) generated by UCNPs-Ce6-mediated PDT can induce autophagy to inhibit the expression of pro-inflammatory factor in M1 peritoneal macrophages. METHODS: Peritoneal macrophages were collected from C57/BL6 mice injected with 3% thioglycollate broth medium and induced by lipopolysaccharides and interferon-gamma. Intracellular ROS production was assessed by 2'-7'-dichloroflorescein diacetate and flow cytometry. Autophagy was assayed by western blot, transmission electron microscopy and immunofluorescence. Pro-inflammatory cytokines were detected by enzyme-linked immunosorbent assay and western blot. RESULTS: Model M1 peritoneal macrophages were established after 24 h induction. Protein expression levels of LC3 II and Beclin1, and degradation of p62 increased and peaked at 2 h in the PDT group. Meanwhile, levels of inflammatory cytokines iNOS, IL-12, and TNF-alpha markedly decreased after PDT. The increase in autophagy levels and decrease in pro-inflammatory cytokines were significantly inhibited by 3-methyladenine. Furthermore, ROS generated by UCNPs- Ce6 mediated PDT activated autophagy. The expression of autophagy related-protein and inflammatory cytokines iNOS, IL-12, and TNF-alpha were inhibited by the ROS inhibitor N-acetyl cysteine. CONCLUSION: ROS generated by UCNPs-Ce6-mediated PDT activated autophagy and inhibited the expression of pro-inflammatory factors of M1 peritoneal macrophage via the PI3K/AKT/mTOR signaling pathway.",
              "Recently, it has been found that the level of urinary D-ribose in type 2 diabetes is notably higher than that in age-matched normal control, and D-ribose is more reactive in the glycation than D-glucose and induces oxidative stress. Kaempferol is one of the main bioactive components in Astragalus membranaceus, with numerous physiological actives, such as antioxidant. The present study investigated the protective effects of kaempferol on D-ribose-treated mesangial cells. CCK-8 and LDH assay were used to test cell viability and cell toxicity. Immunofluorescence and flow cytometry were used to detect the AGE formation and ROS accumulation. GSH level was measured to reflect oxidation resistance. Cell apoptosis was evaluated by Hoechst 33258 staining, AO/EB staining, and western blot. Mitochondrial membrane integrity was detected by JC-1 staining, western blot, and RT-PCR. The change of autophagy level was tested by western blot. The results indicated that D-ribose induced not only cell damage and increased AGE formation and ROS accumulation but also GSH depletion. Further studies demonstrated that D-ribose induced mitochondrial depolarization and the activation of caspase-9/3. But kaempferol could partly block these damages. Subsequently, it was confirmed that kaempferol repaired the autophagy disturbance induced by D-ribose, and 3-MA could reverse the protective effect of kaempferol under D-ribose condition. Our study demonstrated that D-ribose induced AGE accumulation and ROS production in mesangial cell and caused mitochondrial apoptosis, but kaempferol could attenuate these changes and its protective effect might be related to the repair of autophagy.",
              "Background: Hyperosmotic stress is an important pathophysiologic condition in diabetes, severe trauma, dehydration, infection, and ischemia. Furthermore, brain neuronal cells face hyperosmotic stress in ageing and Alzheimer's disease. Despite the enormous importance of knowing the homeostatic mechanisms underlying the responses of nerve cells to hyperosmotic stress, this topic has been underrepresented in the literature. Recent evidence points to autophagy induction as a hallmark of hyperosmotic stress, which has been proposed to be controlled by mTOR inhibition as a consequence of AMPK activation. We previously showed that methylglyoxal induced a decrease in the antioxidant proteins thioredoxin 1 (Trx1) and glyoxalase 2 (Glo2), which was mediated by AMPK-dependent autophagy. Thus, we hypothesized that hyperosmotic stress would have the same effect. Methods: HT22 hippocampal nerve cells were treated with NaCl (37, 75, or 150 mM), and the activation of the AMPK/mTOR pathway was investigated, as well as the levels of Trx1 and Glo2. To determine if autophagy was involved, the inhibitors bafilomycin (Baf) and chloroquine (CQ), as well as ATG5 siRNA, were used. To test for AMPK involvement, AMPK-deficient mouse embryonic fibroblasts (MEFs) were used. Results: Hyperosmotic stress induced a clear increase in autophagy, which was demonstrated by a decrease in p62 and an increase in LC3 lipidation. AMPK phosphorylation, linked to a decrease in mTOR and S6 ribosomal protein phosphorylation, was also observed. Deletion of AMPK in MEFs did not prevent autophagy induction by hyperosmotic stress, as detected by decreased p62 and increased LC3 II, or mTOR inhibition, inferred by decreased phosphorylation of P70 S6 kinase and S6 ribosomal protein. These data indicating that AMPK was not involved in autophagy activation by hyperosmotic stress were supported by a decrease in p(S555)-ULK1, an AMPK phosphorylation site. Trx1 and Glo2 levels were decreased at 6 and 18 h after treatment with 150 mM NaCl. However, this decrease in Trx1 and Glo2 in HT22 cells was not prevented by autophagy inhibition by Baf, CQ, or ATG5 siRNA. AMPK-deficient MEFs under hyperosmotic stress presented the same Trx1 and Glo2 decrease as wild-type cells. Conclusion: Hyperosmotic stress induced AMPK activation, but this was not responsible for its effects on mTOR activity or autophagy induction. Moreover, the decrease in Trx1 and Glo2 induced by hyperosmotic stress was independent of both autophagy and AMPK activation.",
              "Honokiol (HKL) is a natural low-molecular-weight biphenolic compound derived from the bark of magnolia trees. Previous studies indicate that HKL exerts potent cardioprotective effects on ischemia/reperfusion (I/R) injury; however, evidence of the further relationship between HKL posttreatment and myocardial I/R injury has not been clearly found. In our study, we explored the protective effect of HKL post treatment on myocardial I/R injury in C57BL/6 mice. We also demonstrated that HKL significantly reduced cellular reactive oxygen species production and attenuated mitochondrial damage in neonatal rat cardiomyocytes exposed to hypoxia/reoxygenation (H/R). In addition, HKL was found to enhance autophagy during I/R or H/R; these effects could be partially blocked by the autophagic flux inhibitor chloroquine. Moreover, our results suggested that enhanced autophagic flux is associated with the Akt signaling pathway. Collectively, our results indicate that HKL posttreatment alleviates myocardial I/R injury and suggest a critical cardioprotective role of HKL in promoting autophagic flux.",
              "AIM: Nucleus pulposus (NP) cell apoptosis induced by oxidative stress is known to be closely involved in the pathogenesis of intervertebral disc (IVD) degeneration. Berberine, a small molecule derived from Rhizoma coptidis, has been found to exert antioxidative activity and preserve cell viability. The present study aims to investigate whether berberine can prevent NP cell apoptosis under oxidative damage and the potential underlying mechanisms. METHODS AND MATERIALS: The effects of berberine on IVD degeneration were investigated both in vitro and in vivo. KEY FINDINGS: Our results showed that berberine significantly mitigated oxidative stress-decreased cell viability as well as apoptosis in human NP cells. Berberine treatment could attenuate oxidative stress-induced ER stress and autophagy in a concentration-dependent manner. With 4-PBA (ER stress specific inhibitor) and 3-MA (autophagy specific inhibitor) administration, we demonstrated that berberine inhibited oxidative stress-induced apoptosis by modulating the ER stress and autophagy pathway. We also found that the IRE1/JNK pathway was involved in the induction of ER stress-dependent autophagy. With Ca(2+) chelator BAPTA-AM utilization, we revealed that oxidative stress-mediated ER stress and autophagy repressed by berberine could be restored by inducing intracellular Ca(2+) dysregulation. Furthermore, in vivo study provided evidence that berberine treatment could retard the process of puncture-induced IVD degeneration in a rat model. SIGNIFICANCE: Our results indicate that berberine could prevent oxidative stress-induced apoptosis by modulating ER stress and autophagy, thus offering a novel potential pharmacological treatment strategy for IVD degeneration.",
              "BACKGROUND Worldwide, ovarian cancer has a high mortality rate due to the difficulty in diagnosing early-stage disease and resistance to chemotherapy agents. Costunolide is a plant-derived sesquiterpene lactone with anti-oxidant properties. This study aimed to investigate the effects of costunolide on cell growth, apoptosis, autophagy, the production of reactive oxygen species (ROS), cleaved caspase-3, and cleaved caspase-9 on the multidrug-resistant ovarian cancer cell line, OAW42-A. MATERIAL AND METHODS The MTT assay determined the proliferation rate of OAW42-A multidrug-resistant ovarian cancer cells and the apoptosis rate was determined using propidium iodide (PI) staining. Autophagy was detected by measuring the expression of LC3 II. Fluorescence flow cytometry was used to measure the levels of reactive oxygen species (ROS) and the mitochondrial membrane potential. Protein expression of LC3 II, beclin 1, cleaved caspase-3, and cleaved caspase-9 were measured by Western blot. RESULTS Costunolide treatment inhibited the growth of OAW42-A cells with an IC(5)(0) of 25 microM, resulted in apoptotic cell death, increased the expression of Bax, and decreased the expression of Bcl-2. Confocal electron microscopy showed that costunolide induced autophagy in the OAW42-A cells. Western blot showed that costunolide treatment of OAW42-A cells increased the expression of the LC3 II, beclin 1, cleaved caspase-3, and cleaved caspase-9. Costunolide treatment significantly increased the levels of ROS and reduced the OAW42-A cell mitochondrial membrane potential. CONCLUSIONS Costunolide inhibited growth, apoptosis, ROS generation, and was associated with loss of mitochondrial membrane potential of OAW42-A multidrug-resistant ovarian cancer cells.",
              "Finding novel therapeutic agent for the treatment of cerebral ischemia is urgently required. These experiments explored the potential roles of 6-Gingerols (6G) in hypoxia-stimulated rat PC-12 cells. Cell viability, apoptosis and its related proteins were studied by the approaches of MTT assay, flow cytometry assay and Western blot analysis, respectively. In addition, whether 6G achieved its functions in hypoxia-induced injury through miR-103 was illustrated. Moreover, the associated signalling pathways were investigated. Obviously, hypoxia treatment blocked cell viability and enhanced apoptosis while this trend was ameliorated by 6G. Then we observed that hypoxia administration up-regulated miR-103 expression and 6G could further increase miR-103 expression in hypoxia-stimulated PC-12 cells. Inhibition of miR-103 attenuated the neuroprotective effects of 6G on hypoxia-treated PC-12 cells. Moreover, Bcl2/adenovirus EIB 19kD-interacting protein 3 (BNIP3) was a target of miR-103 and BNIP3 upregulation also attenuated the neuroprotective impact of 6G on hypoxia-treated PC-12 cells. Hypoxia activated the p38MAPK and JNK pathways were inactivated by 6G. To sum up, 6G protected hypoxia-stimulated PC-12 cells through miR-103-mediatated down-regulation of BNIP3 by inhibiting p38 MAPK and JNK pathways. Highlights 6-Gingerols (6G) is a promising agent for cerebral ischemia therapy. The neuroprotective effects of 6G are mediated by miR-103 and BNIP3. Up-regulation of miR-103 exerts neuroprotective effects.",
              "Background: Gadolinium-based nanoparticles (GdNPs) have been used as theranostic sensitizers in clinical radiotherapy studies; however, the biomechanisms underlying the radio-sensitizing effects of GdNPs have yet to be determined. In this study, ultra-small gadolinium oxide nanocrystals (GONs) were employed to investigate their radiosensitizing effects and biological mechanisms in non-small-cell lung cancer (NSCLC) cells under X-ray irradiation. Method and materials: GONs were synthesized using polyol method. Hydroxyl radical production, oxidative stress, and clonogenic survival after X-ray irradiation were used to evaluate the radiosensitizing effects of GONs. DNA double-strand breakage, cell cycle phase, and apoptosis and autophagy incidences were investigated in vitro to determine the radiosensitizing biomechanism of GONs under X-ray irradiation. Results: GONs induced hydroxyl radical production and oxidative stress in a dose- and concentration-dependent manner in NSCLC cells after X-ray irradiation. The sensitizer enhancement ratios of GONs ranged between 19.3% and 26.3% for the NSCLC cells under investigation with a 10% survival rate compared with that of the cells treated with irradiation alone. Addition of 3-methyladenine to the cell medium decreased the incidence rate of autophagy and increased cell survival, supporting the idea that the GONs promoted cytostatic autophagy in NSCLC cells under X-ray irradiation. Conclusion: This study examined the biological mechanisms underlying the radiosensitizing effects of GONs on NSCLC cells and presented the first evidence for the radiosensitizing effects of GONs via activation of cytostatic autophagy pathway following X-ray irradiation.",
              "Purpose: The beneficial, neuroprotective effects of curcumin against ischemia-reperfusion injury have been demonstrated. In the present study, whether curcumin exerts neuroprotective effects associated with the inhibition of autophagy and hypoxia inducible factor-1alpha (HIF-1alpha) was investigated. Materials and methods: PC12 cellular model of oxygen glucose deprivation/reperfusion (OGD/R) has been developed to mimic cerebral ischemia-reperfusion injury. Cell viability was evaluated using the CellTiter 96((R)) AQueous One Solution Cell Proliferation Assay. Apoptosis was assessed using flow cytometry. The expression levels of HIF-1alpha and autophagy-associated proteins, LC3 and P62, were examined using Western blot. The autophagy flux was quantitatively estimated based on the number of autophagic compartments using fluorescence microscopy. In addition, 3-methyladenine (3-MA) was administered to PC12 cells to investigate how autophagy affects HIF-1alpha. Moreover, the inhibitory effects of HIF-1alpha on autophagy activation level were examined. Results: In this study, curcumin decreased the death and apoptosis of cells, and inhibited autophagy and HIF-1alpha under OGD/R conditions, consistent with 3-MA treatment or HIF-1alpha downregulation. Moreover, inhibition of autophagy caused a decrease in HIF-1alpha, and the attenuation of HIF-1alpha induced autophagy suppression under OGD/R conditions. Conclusion: The results of this study showed that curcumin exerts neuroprotective effects against ischemia-reperfusion, which is associated with the regulation of the reciprocal function between autophagy and HIF-1alpha.",
              "BACKGROUND: Sub-chronic arsenic (arsenite) exposure-induced oxidative toxicity leads to adverse effects in various organ systems, especially the kidney. Copper sulphate (Cu(2+)), known for its extensive uses in agriculture, has also been reported to have pro-oxidation properties. Both of these two potential toxic elements can bio-accumulate through food chain, thus endangering human health. However, their interaction study in the kidney is scanty. AIM: To investigate the synergism effects of Cu(2+) in arseniasis-elicited oxidative stress and cascaded renal injury in chickens. RESULTS: Arsenite intoxication decreased renal antioxidant system along with ATPases. Arsenite exposure also significantly elicited disequilibrium of mitochondrial homeostasis, accompanying by elevated apoptotic and autophagic cell death. The disturbed morphological and ultrastructural changes further corroborated arsenite nephrotoxicity. These anomalies aligned with the findings in Cu(2+) groups, which co-administrated with arsenic further deteriorated these pathological changes. This synergism was achieved partially via the inactivation of phosphoinositide-3-kinase/protein kinase b/mammalian target of rapamycin (PI3K/AKT/mTOR) pathway through the activation of P53. CONCLUSIONS: Copper excess and arsenic exposure can function independently or cooperatively to affect oxidative stress, mitochondrial dynamics and programmed cell death. These results highlighted the need to take precautions against copper and arsenic co-exposure when considering their impact in susceptible animals/populations.",
              "Cell growth is controlled by a lysosomal signalling complex containing Rag small GTPases and mammalian target of rapamycin complex 1 (mTORC1) kinase. Here, we carried out a microscopy-based genome-wide human short interfering RNA screen and discovered a lysosome-localized G protein-coupled receptor (GPCR)-like protein, GPR137B, that interacts with Rag GTPases, increases Rag localization and activity, and thereby regulates mTORC1 translocation and activity. High GPR137B expression can recruit and activate mTORC1 in the absence of amino acids. Furthermore, GPR137B also regulates the dissociation of activated Rag from lysosomes, suggesting that GPR137B controls a cycle of Rag activation and dissociation from lysosomes. GPR137B-knockout cells exhibited defective autophagy and an expanded lysosome compartment, similar to Rag-knockout cells. Like zebrafish RagA mutants, GPR137B-mutant zebrafish had upregulated TFEB target gene expression and an expanded lysosome compartment in microglia. Thus, GPR137B is a GPCR-like lysosomal regulatory protein that controls dynamic Rag and mTORC1 localization and activity as well as lysosome morphology.",
              "BACKGROUND: To investigate the effects of the Alisma and Rhizoma decoction on nonalcoholic steatohepatitis (NASH) and to further shed light on the underlying mechanisms of the actions of the Alisma and Rhizoma decoction. METHODS: Plasma alanine aminotransferase (ALT) content was determined and liver inflammation and fibrosis were evaluated. Intrahepatocellular malondialdehyde and superoxide dismutase contents were determined using commercially available kits Furthermore, alpha-SMA expression in liver tissues was examined by immunohistochemistry and LC3-II was detected by immunoblotting assays. RESULTS: Mice receiving the Alisma and Rhizoma decoction by gastric lavage had significantly lower plasma ALT content and markedly higher hepatic superoxide dismutase activity than mice receiving the methionine-choline deficient (MCD) diet. Furthermore, the decoction aborted MCD-induced increase in liver malondialdehyde content. Immunohistochemistry showed that the decoction suppressed hepatic alpha-SMA expression. Our transmission electronic microscopy revealed that the decoction markedly reduced the number of autophagosomes and immunoblotting assays showed that the decoction caused a dose-dependent decrease in LC3-II in hepatic tissues. CONCLUSION: The Alisma and Rhizoma decoction lessens NASH-associated liver injuries by modulating oxidative stress and autophagy in hepatocytes of mice fed with MCD.",
              "Tumor cells possess a high metabolic plasticity, which drives them to switch on the anaerobic glycolysis and lactate production when challenged by hypoxia. Among the enzymes mediating this plasticity through bidirectional conversion of pyruvate and lactate, the lactate dehydrogenase A (LDHA) and lactate dehydrogenase B (LDHB), are indicated. LDHA has a higher affinity for pyruvate, preferentially converting pyruvate to lactate, and NADH to NAD(+) in anaerobic conditions, whereas LDHB possess a higher affinity for lactate, preferentially converting lactate to pyruvate, and NAD(+) to NADH, when oxygen is abundant. Apart from the undisputed role of LDHA and LDHB in tumor cell metabolism and adaptation to unfavorable environmental or cellular conditions, these enzymes participate in the regulation of cell death. This review presents the latest progress made in this area on the roles of LDHA and LDHB in apoptosis and autophagy of tumor cells. Several examples of how LDHA and LDHB impact on these processes, as well as possible molecular mechanisms, will be discussed in this article. The information included in this review points to the legitimacy of modulating LDHA and/or LDHB to target tumor cells in the context of human and veterinary medicine.",
              "Melanogenesis is the sequential process of melanin production by melanocytes in order to protect the skin from harmful stimuli. Melanogenesis is disrupted by radiation exposure, which results in the differentiation of melanocytes into melanoma. Recently, some methods have been developed to maintain the instability of melanogenesis in melanoma by activating cellular autophagy. However, there is still a lack of knowledge about how autophagy is involved in the regulation of melanogenesis in melanoma cells. Here, we used rottlerin as an autophagy inducer to investigate the role of the cyclic adenosine monophosphate (cAMP)/cAMP response element binding (CREB) signaling pathway in melanogenesis. We found that rottlerin can inhibit melanin production by targeting cAMP, which is initially activated by alpha-melanocyte stimulating hormone (alpha-MSH). Our findings suggest that rottlerin has a pivotal role as an autophagy inducer in the regulation of melanogenesis by targeting the cAMP/CREB signaling pathway.",
              "Ganoderma lucidum is a multi-purpose plant medicine that is homologous to functional food. The most attractive properties of G. lucidum are its immunomodulatory and antitumour activities, which are mainly attributed to the following two major active components: G. lucidum polysaccharides and G. lucidum triterpenoids (GLTs). GLTs are effective as supplemental therapies and improve health when combined with other medications to treat hepatitis, fatigue syndrome, and prostate cancer. However, research investigating the mechanism and application of G. lucidum or GLTs in the treatment of diseases remains preliminary in terms of both the utilization efficacy and product type. This review offers comprehensive insight into the pharmacological activities of GLTs and their potential applications in the development of functional foods and nutraceuticals. Specifically, 83 GLTs were selected, and their molecular structures and chemical formulas were described. We also describe 7 ganoderic acids that are currently at different stages of clinical trials (ganoderic acids A, C2, D, F, DM, X and Y). The related pharmacodynamic mechanisms and targeted signalling proteins were further analysed. Notably, the specific relationship between autophagy and apoptosis induced by ganoderic acid DM is summarized here for the first time.",
              "Objective To study the mechanism of p38/myocyte enhancer factor 2C (p38/MEF2C) pathway regulating synapse before and after autophagy intervention in the prefrontal cortex of autistic rats. Methods An animal model of autism was induced by intraperitoneal injection of valproic acid (VPA) at 12.5 days of gestation in Wistar rats. They were treated with normal saline or VPA. The offspring of the saline treatment group served as a control group. The offspring of the VPA treatment group were randomly divided into the model group, 5 mg/kg 3-methyladenine (3-MA) group, and 5 mg/kg rapamycin (Rap) autophagy enhanced group, and the treatment time of each group was from the 35th day of birth to the 42nd day of birth. Western blot analysis was used to detect the protein levels of p38, phosphorylated p38 (p-p38), MEF2C, synaptic vesicle protein (SYN), postsynaptic density 95 (PSD-95), and gephyrin protein in the prefrontal cortex of rats; immunohistochemical staining was used to detect the expression and distribution of SYN, PSD-95 and gephyrin in the prefrontal cortex, and semi-quantitative analysis was performed then. Results Compared with the control group, the developmental and behavioral test showed that the model group had developmental lag and social disorder. Compared with the model group, the Rap group shoed improved social disorder, and the 3-MA group could aggravate social disorder. Compared with the control group, the expression of p38, p-p38, MEF2C was down-regulated, the expression of SYN and PSD-95 protein was up-regulated, and the expression of gephyrin was down-regulated. Compared with the model group, the expression of p38, p-p38, MEF2C in the Rap group was up-regulated, the levels of SYN and PSD-95 protein were down-regulated, and the level of gephyrin protein was up-regulated, while that in 3-MA group was opposite. Compared with the control group, the number of SYN and PSD-95 positive cells in the model group increased, and the number of gephyrin positive cells decreased. Compared with the model group, the number of SYN and PSD-95 positive cells in the Rap group decreased, and the number of gephyrin-positive cells increased, and the 3-MA group was opposite. Conclusion The p38/MEF2C signaling pathway is inhibited in the prefrontal cortex of rats with autism, which can regulate the expression of synaptic related proteins and improve autistic behavior by enhancing autophagy to activate the p38/MEF2C signaling pathway.",
              "Objective To explore the possible effect of C-X-C motif chemokine ligands13 (CXCL13) on the autophagy and proliferation of human mesenchymal stem cells (hBMSCs) and the underlying mechanisms. Methods The hBMSCs were divided into control group, hypoxia group, CXCL13 treatment group, and CXCL13 combined with SB203580 group. Except the control group, the rest of cells were cultured under the established hypoxia condition of 920 mL/L N2, 30 mL/L O2 and 50 mL/L CO2. The cells of CXCL13 treatment group were stimulated with 50 ng/mL recombinant CXCL13. In the combination group, the cells were preincubated with 20 mumol /L of MAPK inhibitor SB203580 for 30 minutes and subsequently treated with 50 ng/mL recombinant CXCL13. MTT assay was used to determine the proliferation of hBMSCs. The apoptosis rate of hBMSCs was analyzed by annexin V-FITC/PI double labeling and flow cytometry. The protein levels of nuclear factor-kappaB (NF-kappaB) and beclin-1 were measured by Western blot analysis. ELISA was used to measure the content of autophagy-related protein 7 (ATG7) in cell lysis buffer. Results Compared with the hypoxia group and the combination group. The cell proliferation, beclin-1 and NF-kappaB protein levels increased significantly in the CXCL13 treatment group however, the apoptosis rate decreased, and the level of ATG7 increased significantly in cell lysate. Conclusion Under hypoxic condition, CXCL13 promotes autophagy and proliferation of hBMSCs through the MAPK/NF-kappaB signaling pathway.",
              "Aconitine, a major aconitum alkaloid, is well known for its high toxicity that induces severe arrhythmias and neurological symptoms. One mechanism of aconitine-induced toxic responses is the induction of apoptosis. Apoptosis and autophagy are interconnected processes and the two pathways share critical components. In this study, we investigated the role of autophagy in aconitine-induced toxicity using mouse model. 120 mice were randomly divided into 4 experimental groups (normal saline), low dose group (0.14mumol/L), medium dose group (0.28mumol/L) and high dose group (0.56mumol/ L). 30 mice in each group were administered with aconitine (lavage) for 30days. The livers were collected for analysis of autophagy-related proteins by Western blotting. The expression of LC3II/LC3I ratio and Beclin 1 were found to increase and then decrease with the highest expression at 10days and the p62 showed a time-dependent decreases. Autophagy is regulated by the mTOR pathway, we further analyzed the effects of aconitine on this pathway and found aconitine inhibited, phosphorylation of p-PI3K, p-Akt and p-mTOR. The p-p70s6k and p-4EBP1 which are downstream of mTOR were concomitantly decreased. These results suggest that aconitine induce autophagy in mouse liver. The PI3K/Akt/mTOR signaling pathway is involved in the regulation of aconitine-induced autophagy in the liver of mice.",
              "An update on pathogenesis of Alzheimer's disease, the present document describes the crosstalk between autophagy and inflammation in Alzheimer's disease progression by assessing key findings and identifying gaps in the current knowledge. Inflammation mediators can disrupt the clearance of misfolded proteins. It is well defined that misfolded protein accumulation and aberrant level of inflammatory mediators in the brain can trigger Alzheimer's disease. Studies indicated that restoring the inflammatory and autophagy mediators improves Alzheimer's disease. This presents a problem: How inflammation interacts with the protein clearance system, autophagy, in the brain. Here, we describe an update on the link between inflammation and autophagy pathways. Some of the vital challenges for the presented link included investigation of the questions: What is the role of inflammation mediators in the brain under various conditions; and how altered level of inflammatory mediators affects the performance of autophagy at molecular level.",
              "Several studies indicate that the cytosolic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has pleiotropic functions independent of its canonical role in glycolysis. The GAPDH functional diversity is mainly due to post-translational modifications in different amino acid residues or due to protein-protein interactions altering its localization from cytosol to nucleus, mitochondria or extracellular microenvironment. Non-glycolytic functions of GAPDH include the regulation of cell death, autophagy, DNA repair and RNA export, and they are observed in physiological and pathological conditions as cancer and neurodegenerative disorders. In disease, the knowledge of the mechanisms regarding GAPDH-mediated cell death is becoming fundamental for the identification of novel therapies. Here, we elucidate the correlation between autophagy and GAPDH in cancer, describing the molecular mechanisms involved and its impact in cancer development. Since autophagy is a degradative pathway associated with the regulation of cell death, we discuss recent evidence supporting GAPDH as a therapeutic target for autophagy regulation in cancer therapy. Furthermore, we summarize the molecular mechanisms and the cellular effects of GAPDH aggregates, which are correlated with mitochondrial malfunctions and can be considered a potential therapeutic target for various diseases, including cancer and neurodegenerative disorders.",
              "The immunotoxicity of synthetic pyrethroid (SPs) has garnered much attention, and our previous research demonstrated that beta-CYP causes immunotoxicity and oxidative stress in macrophages. Nevertheless, the underlying mechanism remains largely unknown. In this study, the murine macrophage RAW 264.7cells and murine peritoneal macrophages (PMs) were exposed to beta-CYP. The results showed that beta-CYP elevated intracellular ROS levels in both RAW 264.7cells and PMs. Exposure to beta-CYP also caused mitochondrial dysfunction with reduced mitochondrial membrane potential (MMP), intracellular ATP level and mitochondrial DNA (mtDNA) content in the two cell types. In addition, exposure of RAW 264.7cells to beta-CYP for 12h and 24h enhanced autophagy, with elevated Beclin1, Rab7, Lamp1 and LC3-II expression levels, while 48h of exposure attenuated autophagy. In contrast, exposure of PMs to beta-CYP for 12h promoted autophagy, whereas exposure for 24h and 48h impaired autophagy. Cotreatment with an antioxidant, N-acetyl-L-cysteine (NAC), partially blocked the reduced MMP, intracellular ATP level and autophagy disturbance. Moreover, cotreatment with an autophagy agonist, rapamycin (RAPA), partially blocked mitochondrial dysfunction and oxidative stress in the two cell types, whereas cotreatment with an autophagy inhibitor, 3-methyladenine (3-MA), augmented the abovementioned toxic effects. Furthermore, mitochondrial ROS levels in both RAW 264.7cells and PMs were elevated by exposure to beta-CYP, and molecular docking showed that beta-CYP docked with mouse respiratory chain complex I by binding to the ND2, ND4, and ND5 subunits of the protein complex. Taken together, the data obtained in the present study demonstrate that oxidative stress partially mediates mitochondrial dysfunction and autophagy disturbance upon exposure to beta-CYP in macrophages, and autophagy plays a protective role against the toxic effects.",
              "Atg5, as a switch of cell autophagy and apoptosis, plays an important regulatory role in the occurrence and development of autophagy. Atg5 has been reported to involve the autophagy process but little in the apoptotic process. Here, we constructed an Atg5(-/-) DF-1 cell line using the CRISPR/Cas9 assay and confirmed the significant difference in growth kinetics between Atg5(-/-) DF-1 cells and wild-type DF-1 cells. Importantly, we found that Atg5 suppresses the cellular proliferation and induce the apoptosis in DF-1 cells by Hoechst's staining, flow cytometry, and caspase activity assay. All these findings indicated that Atg5 plays an important role in the proliferation of DF-1 cells. On the other hand, we compared the expression of autophagy key proteins LC3 and P62 in Atg5 knockout cells and wild-type cells, and detected the aggregation point distribution of LC3 protein in cells by laser confocal technique; our results showed that Atg5 knockout inhibited autophagy compared with wild-type cells. The present findings further help to resolve the molecular mechanisms regulating Atg5 autophagy and apoptosis.",
              "Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potent cancer cell apoptosis-inducing factor that can induce apoptosis in a variety of cancer cells. However, resistance to TRAIL in cancer cells is a huge obstacle in creating effective TRAIL-targeted clinical therapies. Thus, agents that can either enhance the effect of TRAIL or overcome its resistance are needed. In this study, we combined TRAIL with SNX-2112, an Hsp90 inhibitor we previously developed, to explore the effect and mechanism that SNX-2112 enhanced TRAIL-induced apoptosis in cervical cancer cells. Our results showed that SNX-2112 markedly enhanced TRAIL-induced cytotoxicity in HeLa cells, and this combination was found to be synergistic. Additionally, we found that SNX-2112 sensitized TRAIL-mediated apoptosis caspase-dependently in TRAIL-resistant HeLa cells. Mechanismly, SNX-2112 downregulated antiapoptosis proteins, including Bcl-2, Bcl-XL, and FLIP, promoted the accumulation of reactive oxygen species (ROS), and increased the expression levels of p-JNK and p53. ROS scavenger NAC rescued SNX-2112/TRAIL-induced apoptosis and suppressed SNX-2112-induced p-JNK and p53. Moreover, SNX-2112 induced the upregulation of death-receptor DR5 in HeLa cells. The silencing of DR5 by siRNA significantly decreased cell apoptosis by the combined effect of SNX-2112 and TRAIL. In addition, SNX-2112 inhibited the Akt/mTOR signaling pathway and induced autophagy in HeLa cells. The blockage of autophagy by bafilomycin A1 or Atg7 siRNA abolished SNX-2112-induced upregulation of DR5. Meanwhile, ROS scavenger NAC, JNK inhibitor SP600125, and p53 inhibitor PFTalpha were used to verify that autophagy-mediated upregulation of DR5 was regulated by the SNX-2112-stimulated activation of the ROS-JNK-p53 signaling pathway. Thus, the combination of SNX-2112 and TRAIL may provide a novel strategy for the treatment of human cervical cancer by overcoming cellular mechanisms of apoptosis resistance.",
              "Chronic exposure to cigarette smoke (CS) causes structural and functional changes in the respiratory tract. It is a major risk factor for cardiovascular and systemic pulmonary diseases. The aim of this study was to investigate the effect of acute CS exposure (2 h) on oxidative stress, heat shock protein 70 (HSP70) expression, autophagy (LC3 expression), and oxidative stress (DCF fluorescence) in human alveolar epithelial cell line A549. Cell culture medium was conditioned with CS using commercial cigarettes, and A549 cells were grown in modified media for 2 h. In some experiments, A549 cells were pretreated with 100 muM of L-buthionine-sulfoximine (BSO) for 24 h to induce glutathione (GSH) depletion. In the cells grown in CS-conditioned medium, GSH was depleted by more than 30%, and reactive oxygen species were increased. Moreover, there was a considerable overexpression of HSP70 and a substantial accumulation of LC3. Similar changes were found when the cells were pretreated with BSO. We conclude that the short-term exposure of epithelial cells to CS increases oxidative stress that entails enhanced autophagy activity.",
              "Prevention of inflammatory bowel disease (IBD) relies on tight control of inflammatory, cell death and autophagic mechanisms, but how these pathways are integrated at the molecular level is still unclear. Here we show that the anti-inflammatory protein A20 and the critical autophagic mediator Atg16l1 physically interact and synergize to regulate the stability of the intestinal epithelial barrier. A proteomic screen using the WD40 domain of ATG16L1 (WDD) identified A20 as a WDD-interacting protein. Loss of A20 and Atg16l1 in mouse intestinal epithelium induces spontaneous IBD-like pathology, as characterized by severe inflammation and increased intestinal epithelial cell death in both small and large intestine. Mechanistically, absence of A20 promotes Atg16l1 accumulation, while elimination of Atg16l1 or expression of WDD-deficient Atg16l1 stabilizes A20. Collectively our data show that A20 and Atg16l1 cooperatively control intestinal homeostasis by acting at the intersection of inflammatory, autophagy and cell death pathways.",
              "Cancer-associated fibroblasts (CAFs) plays an essential role in cancer cell growth, metabolism and immunoreaction. Autophagy is an intracellular self-degradative process that balances cell energy source and regulates tissue homeostasis. Targeting autophagy has gained interest with multiple preclinical and clinical trials, such as the pharmacological inhibitor chloroquine or the inducer rapamycin, especially in exploiting its ability to modulate the secretory capability of CAFs to enhance drug delivery or inhibit it to prevent its influence on cancer cell chemoresistance. In this review, we summarize the reports on autophagy in cancer-associated fibroblasts by detailing the mechanism and role of autophagy in CAFs, including the hypoxic-autophagy positive feedback cycle, the metabolic cross-talk between CAFs and tumors induced by autophagy, CAFs secreted cytokines promote cancer survival by secretory autophagy, CAFs autophagy-induced EMT, stemness, senescence and treatment sensitivity, as well as the research of antitumor chemicals, miRNAs and lncRNAs. Additionally, we discuss the evidence of molecules in CAFs that are relevant to autophagy and the contribution to sensitive treatments as a potential target for cancer treatment.",
              "OBJECTIVE: To identify the significance of autophagy in lupus nephritis (LN). METHODS: The number of autophagosomes in podocytes was counted and the expression of multiple molecular markers associated with autophagy was evaluated in LN specimens: in renal biopsy specimens from 90 patients with LN and 15 healthy controls, autophagosomes in podocytes were counted using transmission electron microscopy and the expression levels of four autophage related proteins including Beclin-1, microtubule-associated protein light chain 3 (LC3), autophagy-related gene 7 (Atg7), and UNC-51-like kinase 1 (ULK1) were measured using immunohistochemistry. RESULTS: The number of autophagosomes in patients with LN types III, IV, and combined V-IV type were significantly higher than in controls (p < 0.0001; p < 0.0001; p = 0.009, respectively). However, the autophagosomes numbers in patients with II and V types LN were significantly lower than controls (both p < 0.0001). Various levels of marker expression were identified, and they correlated significantly with LN pathology classifications. Moreover, the percentage of marker expression in LN types III, IV and V-IV were significantly higher than controls (p < 0.05), while that in types II and V were lower than controls, although the difference for LC3 and ULK1 was not statistically significant. CONCLUSIONS: Autophagy activity and expression pattern of autophagy-related markers in podocytes were significantly positively correlated with LN of types III, IV, and V-IV, but negatively correlated with II and V types. Therefore autophagy could be a useful predictor of LN pathology type, and be informative and helpful in the development of treatment strategies in clinical settings.",
              "Objective: To explore the effect of aspirin combined with metformin on the apoptosis of thyroid cancer TPC-1 cells and its mechanism. Methods: The proliferation and apoptosis of TPC-1 cells treated with different concentrations of aspirin and metformin were detected using cell count kit-8 (CCK-8) assay and flow cytometry, respectively. Western blot was used to detect the expressions of microtubule-associated protein light chain 3 (LC3), p62 and cysteinyl aspartate specific proteinase 3 (caspase-3) after treatment with aspirin, metformin and 3-Methyladenine (3-MA). Results: The relative cell viability of TPC-1 cells treated with 0.5, 1.0, 2.0, 4.0 mmol/L aspirin for 24 and 48 hours were (85.6+/-9.1)%, (79.9+/-8.6)%, (57.0+/-5.3)%, (55.7+/-5.4)%; (76.7+/-2.8)%, (75.4+/-6.1)%, (46.1+/-4.1)%, (36.3+/-3.2)%, respectively. The value of half maximal inhibitory concentration (IC(50)) for 24 and 48 hours were 4.297 mmol/L, 2.133 mmol/L, respectively. The apoptotic rate in the 1 mmol/L aspirin treatment group and negative control group were (29.2+/-8.5)%, (4.2+/-2.9)%, respectively (P<0.05). Moreover, treatment with metformin increased the protein expression of LC3/ ratio, and decreased the expression of p62, while treatment with aspirin decreased the expression of LC3/ ratio and increased the expression of p62. The relative cell viability of TPC-1 cells treated with metformin, 3-MA, an autophagy inhibitor, and 3-MA combined with metformin were (73.2+/-9.2)%, (95.8+/-3.3)%, (59.9+/-9.2)%, respectively. The apoptotic rates in these groups were (35.5+/-1.5)%, (12.3+/-1.4)%, (49.9+/-5.4)%, respectively. Compared with the metformin group, the relative cell viability in metformin combined with 3-MA group was significantly lower while the apoptotic rate was higher (P<0.05), which indicated that treatment with 3-MA enhanced the metformin-induced apoptosis of TPC-1 cells. The relative cell viability of TPC-1 cells in metformin group, aspirin group, metformin combined with aspirin group were (87.3+/-11.8)%, (85.7+/-9.6)%, (72.4+/-8.8)%, respectively. The apoptotic rates in these groups were (29.7+/-4.0)%, (30.5+/-6.5)%, (52.5+/-4.6)%, respectively. Compared with the metformin or aspirin group, the relative cell viability in metformin combined with aspirin group was significantly lower, while the apoptotic rate was higher (P<0.05), which indicated that aspirin enhanced the metformin-induced apoptosis of TPC-1 cells. Conclusions: Our findings indicate that metformin-mediated autophagy plays a protective role in metformin-induced apoptosis and proliferation inhibition. Aspirin enhances the metformin-induced apoptosis of thyroid cancer TPC-1 cells through inhibition of autophagy.",
              "UbiA prenyltransferase domain-containing protein 1 (UBIAD1) is a vitamin K2 biosynthetic enzyme. We previously showed the lethality of this enzyme in UBIAD1 knockout mice during the embryonic stage. However, the biological effects of UBIAD1 deficiency after birth remain unclear. In the present study, we used a tamoxifen-inducible systemic UBIAD1 knockout mouse model to determine the role of UBIAD1 in adult mice. UBIAD1 knockout resulted in the death of the mice within about 60 days of administration of tamoxifen. The pancreas presented with the most prominent abnormality in the tamoxifen-induced UBIAD1 knockout mice. The pancreas was reduced remarkably in size; furthermore, the pancreatic acinar cells disappeared and were replaced by vacuoles. Further analysis revealed that the vacuoles were adipocytes. UBIAD1 deficiency in the pancreatic acinar cells caused an increase in oxidative stress and autophagy, leading to apoptotic cell death in the tamoxifen-induced UBIAD 1 knockout mice. These results indicate that UBIAD1 is essential for maintaining the survival of pancreatic acinar cells in the pancreas.",
              "This study investigated the efficacy of quercetin (QCT) in combination with sodium butyrate (NaB) in enhancing apoptosis in rat C6 and human T98G glioblastoma cells though blockage of autophagy under nutrient-starvation. The most synergistic doses of the drugs were determined to be 25 microM QCT and 1 mM NaB in both cell lines. After QCT and QCT + NaB treatments, autophagy quantification with acridine orange staining showed a drastic decrease in protective autophagy in the cells under nutrient-starvation. Decrease in autophagy was correlated with decreases in expression of Beclin-1 and LC3B II. Combination treatment increased the morphological signs of apoptosis including membrane blebbing, nuclear fragmentation, and chromatin condensation. Annexin V staining was also performed for detection and quantification of increases in apoptosis. Western blotting results showed that combination of QCT and NaB increased apoptosis by decreasing anti-apoptotic Bcl-2 and increasing pro-apoptotic Bax, decreasing survivin, activating caspase-3, and degrading poly (ADP-ribose) polymerase (PARP). This study demonstrated the therapeutic potentials of a novel combination therapy in inhibiting protective autophagy to enhance apoptosis in rat C6 and human T98G glioblastoma cells.",
              "Mitochondrial dysfunction has been implicated in a wide variety of degenerative diseases, including age-related macular degeneration. Damage to mitochondria and mitochondrial DNA accumulates with age in the postmitotic retinal pigment epithelium (RPE), which could lead to RPE cell death and trigger disease. One possible mechanism for cells to avoid cell death is mitophagy, the targeted clearance of damaged mitochondria by autophagy. Here, we induced mitochondrial damage in human RPE cells (ARPE-19 and hRPE), using antimycin A, an inhibitor of complex III of the electron transport chain, and investigated cellular viability, mitochondrial structure and function, and autophagy activity. We observed that antimycin A evoked dose-dependent cell death, a rapid loss in mitochondrial membrane potential, and a collapse of oxidative phosphorylation. Mitochondria appeared swollen and there was clear damage to their cristae structure. At the same time, cells were undergoing active autophagy and were sensitive to autophagy inhibition by bafilomycin A1 or chloroquine. These results indicate that mitochondrial dysfunction can cause significant RPE damage and that autophagy is an important survival mechanism for cells suffering from mitochondrial damage.",
              "Cardiac remodeling characterized by cardiac fibrosis is a pathologic process occurring after acute myocardial infarction. Fibrosis can be ameliorated by interferon-gamma (IFN-gamma), which is a soluble cytokine showing various effects such as anti-fibrosis, apoptosis, anti-proliferation, immunomodulation, and anti-viral activities. However, the role of IFN-gamma in cardiac myofibroblasts is not well established. Therefore, we investigated the anti-fibrotic effects of IFN-gamma in human cardiac myofibroblasts (hCMs) in vitro and whether indoleamine 2,3-dioxygenase (IDO), induced by IFN-gamma and resulting in cell cycle arrest, plays an important role in regulating the biological activity of hCMs. After IFN-gamma treatment, cell signaling pathways and DNA contents were analyzed to assess the biological activity of IFN-gamma in hCMs. In addition, an IDO inhibitor (1-methyl tryptophan; 1-MT) was used to assess whether IDO plays a key role in regulating hCMs. IFN-gamma significantly inhibited hCM proliferation, and IFN-gamma-induced IDO expression caused cell cycle arrest in G0/G1 through tryptophan depletion. Moreover, IFN-gamma treatment gradually suppressed the expression of alpha-smooth muscle actin. When IDO activity was inhibited by 1-MT, marked apoptosis was observed in hCMs through the induction of interferon regulatory factor, Fas, and Fas ligand. Our results suggest that IFN-gamma plays key roles in anti-proliferative and anti-fibrotic activities in hCMs and further induces apoptosis via IDO inhibition. In conclusion, co-treatment with IFN-gamma and 1-MT can ameliorate fibrosis in cardiac myofibroblasts through apoptosis.",
              "HIV-1 gp120, an important subunit of the envelope spikes that decorate the surface of virions, is known to play a vital role in neuronal injury during HIV-1-associated neurocognitive disorder (HAND), although the pathological mechanism is not fully understood. Our previous studies have suggested that the V3 loop of HIV-1 gp120 (HIV-1 gp120 V3 loop) can induce neuronal apoptosis in the hippocampus, resulting in impairment in spatial learning and memory in Sprague-Dawley (SD) rats. In this study, we demonstrated that autophagy was significantly increased in rat primary hippocampal neurons in response to treatment of HIV-1 gp120 V3 loop. Importantly, HIV-1 gp120 V3 loop-induced autophagy played a dual role in the cell survival and death. An increase in autophagy for a short period inhibited apoptosis of neurons, while persistent autophagy over an extended period of time played a detrimental role by augmenting the apoptotic cascade in rat primary hippocampal neurons. In addition, we found that the HIV-1 gp120 V3 loop induced autophagy via AMPK/mTOR-dependent and calpain/mTOR-independent pathways, and the ERK/mTOR pathway plays a partial role. These findings provide evidence that HIV-1-induced autophagy plays a dual role in the survival and apoptosis of the primary rat hippocampal neurons and persistent autophagy may contribute to the pathogenesis of HAND, and autophagy modulation may represent a potential therapeutic strategy for reducing neuronal damage in HAND.",
              "BACKGROUND: Myocardial fibrosis is a common pathological manifestation of many cardiovascular diseases at the end stage. Autophagy has been demonstrated to play a protective role in the cardiac fibrosis. Our previous studies have demonstrated that the Saponins from Panax japonicus effectively ameliorated the degree of fibrosis in rat acute myocardial ischemia injury model though the mechanisms are not clear. HYPOTHESIS: We hypothesized that Chikusetsusaponin IVa (CS), a major component of Saponins from Panaxjaponicus, may improve isoprenaline induced myocardial fibrosis via AMPK/mTOR/ULK1 mediated autophagy METHODS: Continuous subcutaneous injection of isoproterenol for 21 days was used to induce myocardial fibrosis in mice and high and low doses (15mg/kg and 5mg/kg) of CS was administered by oral gavage to observe the efficacy. Animals were sacrificed 12 h after the last administration and samples were collected. H&E staining, Masson staining and wheat germ agglutinin (WGA) staining were used to evaluate histopathological changes, collagen deposition and myocardial cell hypertrophy. Autophagy-related markers (LC3beta, Beclin1 and p62) and AMPK/mTOR/ULK1 pathway-related markers were evaluated by western blot. RESULTS: CS effectively attenuated isoprenaline-induced myocardial fibrosis in vivo, reduced the heart index, inhibited inflammatory infiltration, decreased collagen deposition and myocardial cell size. CS treatment rescued the expression of autophagy-related markers. CS activated autophagy through the activation of AMPK, which in turn inhibited the phosphorylation of mTOR and ULK1(Ser757), rather than directly phosphorylate ULK1(Ser555) by AMPK. CONCLUSION: Our data demonstrated that CS attenuated isoprenaline-induced myocardial fibrosis by activating autophagy through AMPK/mTOR/ULK1 pathway. Our findings suggested that CS is a potential candidate drug against cardiac fibrosis and have identified potential drug targets for the treatment of heart diseases.",
              "BACKGROUND: Cardiac hypertrophy is an adaptive response of the myocardium to pressure or volume overload. Recent evidences indicate that allicin can prevent cardiac hypertrophy. However, it is not clear whether allicin alleviates cardiac hypertrophy by inhibiting autophagy. PURPOSE: We aimed to investigate the effects of allicin on pressure overload-induced cardiac hypertrophy, and further to clarify the related mechanism. STUDY DESIGN/METHODS: Cardiac hypertrophy was successfully established by abdominal aortic constriction (AAC) in rats, and cardiomyocytes hypertrophy was simulated by angiotensin II (Ang II) in vitro. Hemodynamic parameters were monitored by organism function experiment system in vivo. The changes of cell surface area were observed using HE and immunofluorescence staining in vivoand in vitro, respectively. The expressions of cardiac hypertrophy relative protein (BNP and beta-MHC), autophagy marker protein (LC3-II and Beclin-1), Akt, PI3K and ERK were detected by western blot. RESULTS: Allicin could improve cardiac function, and reduce cardiomyocytes size, and decrease BNP and beta-MHC protein expressions. Further results showed that allicin could lower LC3-II and Beclin-1 protein expressions both in vivo and in vitro experiments. And pharmacological inhibitor of mTOR, rapamycin could antagonize the effects of allicin on Ang II-induced cardiac hypertrophy and autophagy. Simultaneously, allicin could promote the expressions of p-Akt, p-PI3K and p-ERK protein. CONCLUSION: These findings reveal a novel mechanism of allicin attenuating cardiac hypertrophy which allicin could inhibit excessive autophagy via activating PI3K/Akt/mTOR and MAPK/ERK/mTOR signaling pathways.",
              "BACKGROUND: Melanoma is a high fatality skin cancer which lacks effective drugs. Sasanquasaponin, an important sort of constituents in theaceae, has been demonstrated to have potent anti-tumor effect in breast cancer and hepatocellular carcinoma. As a sasanquasaponin, we speculate that Sasanquasaponin III (SQS III) isolated from Schima crenata Korth may also have anti-tumor activity. PURPOSE: This study aims to investigate whether SQS III has anti-melanoma activity and examine the underlying mechanisms of SQS III against melanoma. METHODS/STUDY DESIGNS: The anti-proliferative effect of SQS III was assessed by cells viability assay. Annexin V-FITC/PI double staining assay was utilized for detection of apoptosis. Mitochondrial membrane potential and reactive oxygen species (ROS) production were detected using JC-1 and DCFH-DA assay, respectively. Autophagy was monitored using transmission electron microscopy (TEM) and GFP-LC3 transfection fluorescence analysis. Autophagosome-lysosome fusion and lysosomal degradation were determined using a GFP-LC3 & LAMP1 co-localization assay and DQ-BSA staining. Proteins related to apoptosis and autophagy were analyzed by Western blotting. RESULTS: Our results demonstrated that the SQS III exhibited potent anti-cancer activity in A375 cells by inducing both apoptosis and autophagy. In melanoma cells treated with SQS III, caspases were activated and PARP was cleaved, proving the occurrence of apoptosis. Mechanistic studies indicated that the pro-apoptosis activity of SQS III was mediated by death receptor pathway and mitochondrial dysfunction which was induced by ROS accumulation and reversed by the ROS inhibitor N-acetyl-cysteine (NAC). In addition to triggering apoptosis, SQS III may also cause autophagy in melanoma cells. Our results demonstrated that SQS III induced up-regulated expression of GFP-LC3, autophagosome-lysosomal fusion and lysosomal degradation. Additionally, the ROS accumulation was also involved in the activation of autophagy. Meanwhile, it was also found that after SQS III treatment, the expression of LC3-II was up-regulated and the AKT/mTOR signaling pathway was inhibited. The autophagy inhibitor 3-MA converted cytotoxicity and apoptosis of SQS III in A375 cells, which indicated that autophagy promoted the SQS III-induced apoptosis. CONCLUSION: SQS III showed potent anti-cancer activity by inducing apoptosis and autophagy, which provides insights into its possible use as a therapy for melanoma.",
              "AIMS: Improvements in cancer treatment have significantly extended the lifespan of patients. However, due to the adverse effects of cancer treatment, cancer survivors are at increased risk of cardiovascular complications. Doxorubicin is a widely used spectrum antitumor drug, but the life-threatening side-effect of cardiotoxicity limits its clinical application. Ammonium chloride (NH4Cl), as a heteropolar compound with pH value regulation, can cause intracellular alkalization and metabolic acidosis thus effecting enzymatic activity and influencing the process of biological system. The underlying effect of NH4CL in DOX-induced cardiomyocyte apoptosis and hypertrophy in mice has never been reported before. MAIN METHODS: This study we used DOX to induce cardiac remodeling and dysfunction in mice. Myocardial histology was performed using HE staining. Myocardial cell size was measured by wheat germ agglutinin (WGA) staining. Echocardiographic evaluation of cardiac function, qPCR detection of the mRNA expression of cardiac hypertrophy and inflammation markers. Apoptosis was detected by TUNEL method. Transmission electron microscopy (TEM) was used to detect autophagy. KEY FINDINGS: We found that NH4CL effectively improved DOX-induced cardiomyocyte apoptosis and cardiac dysfunction in mice. Our results showed that NH4CL significantly improved DOX-induced contractile dysfunction, inflammation, apoptosis and autophagy in mice. SIGNIFICANCE: Our results indicate that NH4CL is effective in improving DOX-induced cardiac dysfunction and remodeling. It may therefore be a therapeutic entry point to limit doxorubicin-mediated adverse cardiac reactions.",
              "Salmonella enterica serovar Typhimurium (S. Typhimurium) inhabits a wide range of hosts, including poultry, and causes acute gastroenteritis in humans that may result in death. Superoxide dismutase (SOD) is an important antioxidant enzyme present in nearly all living cells exposed to oxygen. Recently, we reported the novel roles of SOD in serum resistance and biofilm formation in S. Typhimurium. This study was designed to explore the effect of infection with sodA mutant of S. Typhimurium on the autophagic response of macrophages. Murine macrophage cell line RAW264.7 was infected with wild-type (LSM52), a sodA deletion mutant (LSM52DeltasodA) and complemented strain (LSM52CDeltasodA). We found that sodA deletion triggered remarkable autophagic responses in infected cells, shown as higher concentrations of LC3-II or Beclin-1 than those infected with the wild-type or complemented strain during the first hour post-infection in S. Typhimurium. Consistent with these results, the number of viable bacteria in cells infected with the sodA mutant was significantly lower than those infected with wild-type or complemented strains at 1 h, 2 h and 3 h post-infection in S. Typhimurium. All results indicated that infection with sodA mutant of S. Typhimurium leads to up-regulation of autophagy in Raw264.7 macrophages. SIGNIFICANCE AND IMPACT OF THE STUDY: Autophagy plays an important role in Salmonella infection although the role of autophagy in Salmonella infection remains unclear. This study was designed to explore the effect of sodA on the autophagic response of macrophage. We found that infection with sodA mutant of Salmonella Typhimurium could lead to up-regulation of autophagy in Raw264.7 macrophages.",
              "ATG9 vesicles are crucial for autophagy, yet the role of ATG9 remains unclear. In this issue, Judith et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201901115) implicate the BAR protein Arfaptin2 in the loading of PI4-kinase IIIbeta onto ATG9 vesicles for recruitment of ATG13 to the site of autophagosome biogenesis.",
              "The gradual energy dissipation of all organisms allows adapting to energy demands. Pathological situations of uncured diseases such as cancer, diabetes, and other obesity-related diseases are caused by an abrupt energy imbalance. As an energy sensor, AMP-activated kinase (AMPK) can regulate the cellular energy status. In case of increased energy demands or insufficient nutrient supply, cells digest their own interior, which is called autophagy. AMPK-mediated autophagy regulates various metabolic and physiological processes and is dysregulated in different chronic conditions. Because of AMPK's critical role in physiology and pathology, it is an emerging target for both prevention and treatment of these uncured diseases. This review discusses the multifaceted role of AMPK on cancer cell survival and inhibition mechanism. First, we discuss the dual role of AMPK on cancer progression and suppression, and we discuss how different AMPK subunit combinations influence the tumor progression and suppression. Next, we discuss what could be the centering point of AMPK that supports promotion or inhibition of the cancer cell growth. Furthermore, we review the role of connecting mechanism of AMPK-mediated molecular intermediates on cancer cell survival and inhibition pathways. Finally, we discuss how AMPK can affect DNA damage and repairing mechanisms, and immune response of host cell and cancer cells.",
              "Neoplastic transformation is characterized by metabolic rewiring to sustain the elevated biosynthetic demands of highly proliferative cancer cells. To obtain the precursors for macromolecule biosynthesis, cancer cells avidly uptake and metabolize glucose and glutamine. Thus, targeting the availability or metabolism of these nutrients is an attractive anticancer therapeutic strategy. To improve our knowledge concerning how cancer cells respond to nutrient withdrawal, the response to glutamine and/or glucose starvation was studied in human in vitro transformed fibroblasts, deeply characterized at the cellular and molecular level. Concomitant starvation of both nutrients led to rapid loss of cellular adhesion (~16 h after starvation), followed by cell death. Deprivation of glucose alone had the same effect, although at a later time (~48 h after starvation), suggesting that glucose plays a key role in enabling cell attachment to the extracellular matrix. Glutamine deprivation did not induce rapid cell death, but caused a prolonged arrest of cellular proliferation; the cells started dying only 96 h after starvation. Before massive cell death occurred, the effects of all the starvation conditions were reversible. Autophagy activation was observed in cells incubated in the absence of glucose for more than 48 h, while autophagy was not detected under the other starvation conditions. Markers of apoptotic cell death, such as caspase 3, caspase 9 and poly(ADPribose) polymerase 1 (PARP1) proteolytic fragments, were not observed under any growth condition. Glucose and/or glutamine deprivation caused very rapid PARP1 activation, with marked PARP1 (polyADP) ribosylation and protein (polyADP) ribosylation. This activation was not due to starvationinduced DNA doublestrand breaks, which appeared at the late stages of deprivation, when most cells died. Collectively, these results highlight a broad range of consequences of glucose and glutamine starvation, which may be taken into account when nutrient availability is used as a target for anticancer therapies.",
              "Neurodegenerative diseases like Alzheimer's disease, Parkinson's disease and Huntington's disease manifest with the neuronal accumulation of toxic proteins. Since autophagy upregulation enhances the clearance of such proteins and ameliorates their toxicities in animal models, we and others have sought to re-position/re-profile existing compounds used in humans to identify those that may induce autophagy in the brain. A key challenge with this approach is to assess if any hits identified can induce neuronal autophagy at concentrations that would be seen in humans taking the drug for its conventional indication. Here we report that felodipine, an L-type calcium channel blocker and anti-hypertensive drug, induces autophagy and clears diverse aggregate-prone, neurodegenerative disease-associated proteins. Felodipine can clear mutant alpha-synuclein in mouse brains at plasma concentrations similar to those that would be seen in humans taking the drug. This is associated with neuroprotection in mice, suggesting the promise of this compound for use in neurodegeneration.",
              "BACKGROUND: The biology function of antisense intronic long noncoding RNA (Ai-lncRNA) is still unknown. Meanwhile, cancer patients with paclitaxel resistance have limited therapeutic options in the clinic. However, the potential involvement of Ai-lncRNA in paclitaxel sensitivity remains unclear in human cancer. METHODS: Whole transcriptome sequencing of 33 breast specimens was performed to identify Ai-lncRNA EGOT. Next, the role of EGOT in regulation of paclitaxel sensitivity was investigated. Moreover, the mechanism of EGOT enhancing autophagy sensitizes paclitaxel cytotoxicity via upregulation of ITPR1 expression by RNA-RNA and RNA-protein interactions was investigated in detail. Furthermore, upstream transcriptional regulation of EGOT expression was also investigated by co-immunoprecipitation and chromatin immunoprecipitation. Finally, clinical breast specimens in our cohort, TCGA and ICGC were applied to validate the role of EGOT in enhancing of paclitaxel sensitivity. RESULTS: EGOT enhances autophagosome accumulation via the up-regulation of ITPR1 expression, thereby sensitizing cells to paclitaxel toxicity. Mechanistically, on one hand, EGOT upregulates ITPR1 levels via formation of a pre-ITPR1/EGOT dsRNA that induces pre-ITPR1 accumulation to increase ITPR1 protein expression in cis. On the other hand, EGOT recruits hnRNPH1 to enhance the alternative splicing of pre-ITPR1 in trans via two binding motifs in EGOT segment 2 (324-645 nucleotides) in exon 1. Moreover, EGOT is transcriptionally regulated by stress conditions. Finally, EGOT expression enhances paclitaxel sensitivity via assessment of cancer specimens. CONCLUSIONS: These findings broaden comprehensive understanding of the biology function of Ai-lncRNAs. Proper regulation of EGOT may be a novel synergistic strategy for enhancing paclitaxel sensitivity in cancer therapy.",
              "Autophagy is an evolutionarily conserved 'self-eating' process that maintains cellular, tissue, and organismal homeostasis. New studies on autophagy, mediated by subsets of autophagy proteins, are emerging in many physiological and pathological processes. Astragalus membranaceus (AM), also named Huangqi, is one of the fundamental herbs in traditional Chinese medicine and its extracts have been proved to possess many biological activities related to autophagy, including anti-oxidation, anti-inflammation, anticancer, anti-photoaging, and improvement of cardiomyocyte function. Evidence suggests that AM extracts can have therapeutic potential in autophagy dysregulation-associated diseases because of their biological positive effects. Here we will review the literature concerning the effects of AM extracts on autophagy dysregulation-associated diseases.",
              "Fascaplysin is a natural product isolated from marine sponges that exhibits broad anticancer activity. Previous studies revealed that fascaplysin-induced apoptosis and angiogenesis inhibition in vascular endothelial cells contributed to its anticancer activity. Accumulating evidence indicates that autophagy plays a significant role in mediating the function of vascular endothelial cells (VECs) and the response to cancer therapy. However, the effect of fascaplysin on VEC autophagy and the role of autophagy in fascaplysin-induced vascular endothelial cell apoptosis and angiogenesis inhibition are not clear. The present study found that fascaplysin induced autophagy in vascular endothelial cells. Suppression of autophagy using a pharmacological inhibitor (3-methyladenine) or RNA interference of an essential autophagy gene (ATG5) enhanced the cell death and anti-angiogenesis activity of fascaplysin. We further found that fascaplysin significantly increased p8 protein and reactive oxygen species (ROS) levels and decreased mitochondrial membrane potential but had no effect on the mTOR pathway in VECs. Notably, the ROS scavenger N-acetylcysteine inhibited fascaplysin-induced autophagy and increased p8 protein level. Knockdown of p8 by using RNA interference inhibited the autophagy but increased the level of ROS in VECs. Taken together, these data indicated that fascaplysin activated autophagy as a cytoprotective response via ROS and p8 in VECs. Our findings provided important insight into the response of VECs to fascaplysin and may be useful for improving the anticancer efficacy of fascaplysin.",
              "Autophagy is a dynamic recycling process that eliminates damaged proteins and cellular organelles to maintain cellular homeostasis. Aldose reductase (AR) catalyzes conversion of glucose to sorbitol. It also catalyzes the reduction of a broad array of saturated and unsaturated aldehydes. Recently we demonstrated that deletion of AR promotes pathological cardiac remodeling via excessive autophagy; however, the role of AR in starvation-induced autophagy has not been determined. To determine the role of AR in starvation-induced autophagy, WTC57/Bl6 mice were pretreated with the AR inhibitor sorbinil (0.2g/L for 48h) in drinking water, followed by 24h fasting. We found that the sorbinil pretreatment in fed mice did not affect blood glucose levels, whereas, it decreased the blood glucose levels in fasting mice. In comparison with fed mice, the LC3II formation and LCII/LCI ratio were increased in the fasted mice hearts and sorbinil pretreatment further enhanced LC3II formation and LC3II/LC3I ratios in these hearts. Fasting-induced autophagy coincided with AMPK activation in the sorbinil pretreated fasted mice hearts. Autophagy and activation of AMPK was also induced in the gastrocnemius skeletal muscle of sorbinil pre-treated fasted mice. Induction of autophagy in the cardiac tissues of sorbinil pretreated fasted mice was accompanied by increased clearance of 4-hydroxytrans-2-nonenal-protein adducts. Taken together, these results indicate that the inhibition of AR during fasting activates autophagic response, increases clearance of aldehyde-protein adducts, which could serve as a mechanism to maintain cellular homeostasis during starvation.",
              "OBJECTIVE: To investigate the effect of targeting the silent information regulator 2 hemolog 2 (SIRT2) expression on the apoptosis of drug-resistant AML cell line HL-60/A and its mechanism. METHODS: The expression of SIRT2 and antophagy-related protein LCT, P62 in HL-60/A and HL-60 was detected by Western blot, the effect of cytorabine on the apoptosis of HL-60/A cells was detected by using Annexin V/PI double staining after targeting inhibition of SIRT2 expression resulting from transfecting HL-60/A cells with SiRNA. The Western blot and transmission electray microscopy were used to detect the cell autophagy. To further clarify the role of autophragy in the regulatory effect of SIRT2 on the drug-resistance of HL-60/A cells, the autophagy-specific agonist, repamycin, was added into the cell culture medium after SIRT2-siRNA transfection. Then, the autophagy and apoptosis of HL-60/A were detected, respectively. RESULTS: The SIRT2 protein expression obviously increased in HL-60/A cells than that in HL-60 cells. Moreover, the expression rate of LC3II/I was higher, but P62 expression was lower in HL-60/A cells. After siRNA successfully transfecting into HL-60/A cells, quantitative PCR and Western blot should that the expression of SIRT2 significantly decreased. Meawhile, Western blot showed that the expression of LC3 II/I decreased, but P62 increased. Meanwhile, By TEM found that the number of autophagosome also decreased, suggesting the autophagy was inhibited after down-regulation of SIRT2. In addition, the drug senstivity of HL-60/A cells to cytarabine in siRNA-transfection group increased, and the apoptotic rate detected by Annexin V/PI double staining significantly increased. However, after co-culture with rapamycin, the suppressed autophagy in siRNA-trasfect HL-60/A cells was activated, leading to the reappearance of drug resistance of cells to cytarabine, and more significantly decrease of apoptotic rate. CONCLUSION: The high expression of SIRT2 in HL-60/A cells activates the protective autophagy mechanism, which closely related with drug resistance.",
              "BACKGROUND We examined the anticancer potential of anthecotulide against SK-MEL-24 malignant melanoma cells. The apoptotic and autophagic effects of anthecotulide were also investigated. MATERIAL AND METHODS The cell viability of SK-MEL-24 human malignant melanoma cells was evaluated by WST-1 assay. Fluorescence microscopy using acridine orange and ethidium bromide staining, as well as Western blot analysis, were used to study apoptotic effects induced by anthecotulide. Autophagy was assessed by Western blot analysis and fluorescence microscopy. Effects of anthecotulide on cell cycle progression were analyzed by flow cytometry. RESULTS The results revealed that anthecotulide exerts significant growth-inhibitory effects on SK-MEL-24 cells. The IC(5)(0) of anthecotulide against the SK-MEL-24 cells was found to be 10 microM. However, the anticancer effects against the normal cells were minimal (IC50; 100 microM). Investigation of the underlying mechanism revealed that anthecotulide prompts apoptotic cell death of the SK-MEL-24 cells, which was linked with increased expression of Bax and decreased expression of Bcl-2. It also triggered concentration-dependent activation of caspase 3 and 9. Anthecotulide induced autophagy in the SK-MEL-24 cells, which was associated with upregulation of LC3 II and Beclin-1 expression. Anthecotulide also halted the SK-MEL-24 cells at S-phase of the cell cycle and downregulated the expression of Cyclin B1. However, the expression of p27 was upregulated. CONCLUSIONS These results indicate anthecotulide is a potent lead molecule for the treatment of melanoma. In vivo and other related experiments are warranted to further assess this promising drug candidate.",
              "BACKGROUND: Critical illness myopathy (CIM) is associated with severe skeletal muscle wasting and impaired function in intensive care unit (ICU) patients. The mechanisms underlying CIM remain incompletely understood. To elucidate the biological activities occurring at the transcriptional level in the skeletal muscle of ICU patients with CIM, the gene expression profiles, potential upstream regulators, and enrichment pathways were characterized using RNA sequencing (RNA-seq). We also compared the skeletal muscle gene signatures in ICU patients with CIM and genes perturbed by mechanical loading in one leg of the ICU patients, with an aim of reducing the loss of muscle function. METHODS: RNA-seq was used to assess gene expression changes in tibialis anterior skeletal muscle samples from seven critically ill, immobilized, and mechanically ventilated ICU patients with CIM and matched control subjects. We also examined skeletal muscle gene expression for both legs of six ICU patients with CIM, where one leg was mechanically loaded for 10 h/day for an average of 9 days. RESULTS: In total, 6257 of 17,221 detected genes were differentially expressed (84% upregulated; p < 0.05 and fold change >/= 1.5) in skeletal muscle from ICU patients with CIM when compared to control subjects. The differentially expressed genes were highly associated with gene changes identified in patients with myopathy, sepsis, long-term inactivity, polymyositis, tumor, and repeat exercise resistance. Upstream regulator analysis revealed that the CIM signature could be a result of the activation of MYOD1, p38 MAPK, or treatment with dexamethasone. Passive mechanical loading only reversed expression of 0.74% of the affected genes (46 of 6257 genes). CONCLUSIONS: RNA-seq analysis revealed that the marked muscle atrophy and weakness observed in ICU patients with CIM were associated with the altered expression of genes involved in muscle contraction, newly identified E3 ligases, autophagy and calpain systems, apoptosis, and chaperone expression. In addition, MYOD1, p38 MAPK, and dexamethasone were identified as potential upstream regulators of skeletal muscle gene expression in ICU patients with CIM. Mechanical loading only marginally affected the skeletal muscle transcriptome profiling of ICU patients diagnosed with CIM.",
              "In the study, the protective effect of plasma protein from Tachypleus tridentatus (PPTT) on acute kidney injury (AKI) and the related molecular mechanisms were first investigated by Western blotting analyses, TdT-mediated dUTP Nick-End Labeling (TUNEL) assay, and immunohistochemistry. It was found that PPTT had an obviously inhibitory effect on Reactive oxygen species (ROS) in cyclophosphamide (CTX)-exposed mice. Furthermore, results demonstrated that the renal cell death mode is due to inducing apoptosis and autophagy inhibited by dose-dependent PPTT in mice treated with CTX by decreasing the protein expression of bax, beclin-1, and LC3 and increasing the expression of bcl-2. Moreover, the p38 MAPK and PI3K/Akt signaling pathways were observed to take part in the PPTT-induced renal cell growth effect by enhancing the upregulation of the expression of Akt and p-Akt as well as the downregulation of the expression of p38 and p-p38, which indicated a PPTT ameliorating effect on AKI CTX-induced in mice through p38 MAPK and PI3K/Akt signaling pathways. Briefly, this article preliminarily studies the mechanism of the PPTT ameliorating effect on AKI CTX-induced in mice, which helps to provide a reference for PPTT clinical application in AKI therapy.",
              "Autophagy is a cytoprotective mechanism triggered in response to adverse environmental conditions. Herein, we investigated the autophagy process in the oriental river prawn (Macrobrachium nipponense) following hypoxia. Full-length cDNAs encoding autophagy-related genes (ATGs) ATG3, ATG4B, ATG5, and ATG9A were cloned, and transcription following hypoxia was explored in different tissues and developmental stages. The ATG3, ATG4B, ATG5, and ATG9A cDNAs include open reading frames encoding proteins of 319, 264, 268, and 828 amino acids, respectively. The four M. nipponense proteins clustered separately from vertebrate homologs in phylogenetic analysis. All four mRNAs were expressed in various tissues, with highest levels in brain and hepatopancreas. Hypoxia up-regulated all four mRNAs in a time-dependent manner. Thus, these genes may contribute to autophagy-based responses against hypoxia in M. nipponense. Biochemical analysis revealed that hypoxia stimulated anaerobic metabolism in the brain tissue. Furthermore, in situ hybridization experiments revealed that ATG4B was mainly expressed in the secretory and astrocyte cells of the brain. Silencing of ATG4B down-regulated ATG8 and decreased cell viability in juvenile prawn brains following hypoxia. Thus, autophagy is an adaptive response protecting against hypoxia in M. nipponense and possibly other crustaceans. Recombinant MnATG4B could interact with recombinant MnATG8, but the GST protein could not bind to MnATG8. These findings provide us with a better understanding of the fundamental mechanisms of autophagy in prawns.",
              "To explore the effects and molecular mechanisms of mycelium of Cordyceps sinensis(MCs)improving renal tubular epithelial cells aging induced by D-galactose,the renal proximal tubular epithelial cells(NRK-52E cells)of rats in vitro were divided into the normal group(N),the D-gal model group(D),the low dose of MCs group(L-MCs),the medium dose of MCs group(M-MCs)and the high dose of MCs group(H-MCs),and treated by the different measures,respectively.More specifically,the NRK-52E cells in each group were separately treated by 1%fetal bovine serum(FBS)or D-galactose(D-gal,100 mmol.L~(-1))or D-gal(100 mmol.L~(-1))+MCs(20 mg.L~(-1))or D-gal(100 mmol.L~(-1))+MCs(40 mg.L~(-1))or D-gal(100 mmol.L~(-1))+MCs(80 mg.L~(-1)).After the intervention for24 h or 48 h,firstly,the effects of D-gal on the protein expression levels of klotho,P27 and P16,the staining of senescence-associatedbeta-galactosidase(SA-beta-gal)and the activation of adenosine monophosphate activated protein kinase(AMPK)/uncoordinated 51-like kinase 1(ULK1)signaling in the NRK-52E cells were detected,respectively.Secondly,the effects of MCs on the activation of the NRK-52E cells proliferation were investigated,respectively.Finally,the effects of MCs on the protein expression levels of klotho,P27,P16and microtubule-associated protein 1 light chain 3(LC3),the staining of SA-beta-gal and the activation of AMPK/ULK1 signaling in the NRK-52E cells exposed to D-gal were examined severally.The results indicated that,for the NRK-52E cells,D-gal could cause aging,induce the protein over-expression levels of the phosphorylated AMPK(p-AMPK)and the phosphorylated ULK1(p-ULK1)and activate AMPK/ULK1 signaling pathway.The co-treatment of MCs at the medium and high doses and D-gal could significantly ameliorate the protein expression levels of klotho,P27,P16 and the staining of SA-beta-gal,suggesting the anti-cell aging actions.In addition,the cotreatment of MCs at the medium and high doses and D-gal could obviously improve the protein expression levels of LC3,p-AMPK,and p-ULK1,inhibit the activation of AMPK/ULK1 signaling and increase autophagy.On the whole,for the renal tubular epithelial cells aging models induced by D-gal,MCs not only has the in vitro actions of anti-aging,but also intervenes aging process by inhibiting autophagy-related AMPK/ULK1 signaling activation,which may be the novel molecular mechanisms of MCs protecting against aging of the renal tubular epithelial cells.",
              "In our previous study, we proved that a novel Heat shock protein 90 (HSP90) inhibitor 4-(3-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-y l) benzoic acid (DPB) could inhibit A549 lung cancer cell growth via inducing apoptosis. However, whether DPB affects autophagy is still unknown. Here, we investigated the effects of DPB on autophagy and the improved anti-cancer activity in A549 lung cancer cells. Aggregation of LC3-II was observed using laser scanning confocal microscopy in GFP-LC3 stably transfected U87 cells. Autophagy and apoptosis-related protein levels were examined by Western blot analysis. It is suggested that treatment with DPB (5-20 mumol/L) induced mTOR-independent autophagy in dose- and time-dependent manners. Pre-treatment A549 cells with autophagy inhibitor 3-methyladenine (3-MA, 5 mmol/L) enhanced DPB-induced apoptosis. And, DPB inhibited A549 cell growth more effectively in combination with autophagy inhibitors 3-MA (5 mmol/L) or 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO, 30 mumol/L). These results illustrated that as a potential and promising HSP90 inhibitor, DPB could be utilized in the treatment of cancer combined with the autophagy inhibitor.",
              "BACKGROUND: ALKBH5 regulated the malignant behavior of breast cancer and glioblastoma. However, the expression and function of ALKBH5 in epithelial ovarian cancer have not yet been determined. In the present study, we investigated the expression and function of ALKBH5 in epithelial ovarian cancer with respect to its potential role in the tumorigenesis of the disease as well as an early diagnostic marker. METHODS: Immunohistochemistry and western blot were used to detect protein expression. Gene silencing and over-expression experiment were used to study gene function. Cell proliferation assay and Matrigel invasion assays were used to detect cell proliferation and invasion, respectively. The nude mouse tumor formation experiment was used to evaluate the growth of cells in vivo. RESULTS: The expression of ALKBH5 was found to be increased in epithelial ovarian cancer tissue as compared to the normal ovarian tissues. The silencing of ALKBH5 in SKOV3 cells enhanced the autophagy and inhibited the proliferation and invasion in vitro and in vivo, whereas the ectopic expression of ALKBH5 in A2780 cells exerted an opposite effect. Mechanical study revealed that ALKBH5 physically interacted with HuR. ALKBH5 activated EGFR-PIK3CA-AKT-mTOR signaling pathway. Also, ALKBH5 enhanced the stability of BCL-2 mRNA and promoted the interaction between Bcl-2 and Beclin1. CONCLUSION: Overall, the present study identified ALKBH5 as a candidate oncogene in epithelial ovarian cancer and a potential target for ovarian cancer therapy.",
              "BACKGROUND: Glucose-6-phospate dehydrogenase (G6PD) is the limiting enzyme of the pentose phosphate pathway (PPP) correlated to cancer progression and drug resistance. We previously showed that G6PD inhibition leads to Endoplasmic Reticulum (ER) stress often associated to autophagy deregulation. The latter can be induced by target-based agents such as Lapatinib, an anti-HER2 tyrosine kinase inhibitor (TKI) largely used in breast cancer treatment. METHODS: Here we investigate whether G6PD inhibition causes autophagy alteration, which can potentiate Lapatinib effect on cancer cells. Immunofluorescence and flow cytometry for LC3B and lysosomes tracker were used to study autophagy in cells treated with lapatinib and/or G6PD inhibitors (polydatin). Immunoblots for LC3B and p62 were performed to confirm autophagy flux analyses together with puncta and colocalization studies. We generated a cell line overexpressing G6PD and performed synergism studies on cell growth inhibition induced by Lapatinib and Polydatin using the median effect by Chou-Talay. Synergism studies were additionally validated with apoptosis analysis by annexin V/PI staining in the presence or absence of autophagy blockers. RESULTS: We found that the inhibition of G6PD induced endoplasmic reticulum stress, which was responsible for the deregulation of autophagy flux. Indeed, G6PD blockade caused a consistent increase of autophagosomes formation independently from mTOR status. Cells engineered to overexpress G6PD became resilient to autophagy and resistant to lapatinib. On the other hand, G6PD inhibition synergistically increased lapatinib-induced cytotoxic effect on cancer cells, while autophagy blockade abolished this effect. Finally, in silico studies showed a significant correlation between G6PD expression and tumour relapse/resistance in patients. CONCLUSIONS: These results point out that autophagy and PPP are crucial players in TKI resistance, and highlight a peculiar vulnerability of breast cancer cells, where impairment of metabolic pathways and autophagy could be used to reinforce TKI efficacy in cancer treatment.",
              "Obesity, a major risk factor for chronic diseases such as type 2 diabetes (T2D), represents a serious primary health problem worldwide. Dietary habits are of special interest to prevent and counteract the obesity and its associated metabolic disorders, including lipid steatosis. Capsaicin, a pungent compound of chili peppers, has been found to ameliorate diet-induced obesity in rodents and humans. The purpose of this study was to examine the effect of capsaicin on hepatic lipogenesis and to delineate the underlying signaling pathways involved, using HepG2 cells as an experimental model. Cellular neutral lipids, stained with BODIPY493/503, were quantified by flow cytometry, and the protein expression and activity were determined by immunoblotting. Capsaicin reduced basal neutral lipid content in HepG2 cells, as well that induced by troglitazone or by oleic acid. This effect of capsaicin was prevented by dorsomorphin and GW9662, pharmacological inhibitors of AMPK and PPARgamma, respectively. In addition, capsaicin activated AMPK and inhibited the AKT/mTOR pathway, major regulators of hepatic lipogenesis. Furthermore, capsaicin blocked autophagy and increased PGC-1alpha protein. These results suggest that capsaicin behaves as an anti-lipogenic compound in HepG2 cells.",
              "BACKGROUND: The pathological heart contractions, called arrhythmias, especially ventricular fibrillation (VF), are a prominent feature of many cardiovascular diseases leading to sudden cardiac death. The present investigation evaluates the effect of electrically stimulated VF on cardiac functions related to autophagy and apoptotic mechanisms in isolated working rat hearts. METHODS: Each group of hearts was subjected to 0 (Control), 1, 3, or 10 min of spacing-induced VF, followed by 120 min of recovery period and evaluated for cardiac functions, including aortic flow (AF), coronary flow (CF), cardiac output (CO), stroke volume (SV), and heart rate (HR). Hearts were also evaluated for VF effects on infarcted zone magnitude and Western blot analysis was conducted on heart tissue for expression of the apoptotic biomarker cleaved-caspase-3 and the autophagy proteins: p62, P-mTOR/mTOR, LC3BII/LC3BI ratio, and Atg5-12 complexes. RESULTS: Data revealed that VF induced degradation in AF, CF, CO, and SV, which prominently included-variable post-VF capacity for recovery of normal heart rhythm; increased extent of infarcted heart tissue; altered expression of cleaved-caspase-3 suggesting potential for VF-mediated amplification of apoptosis. VF influence on expression of p62, LC3BII/LC3BI, and Atg5-12 proteins was complex, possibly due to differential effects of VF-induced expression on proteins comprising the autophagic program. CONCLUSIONS: VF was observed to cause time-dependent changes in autophagy processes, which with additional analysis under ongoing investigations, likely to yield novel therapeutic targets for the prevention of VF and sudden cardiac death.",
              "Proteinuria is one of the most important clinical features of nephrotic syndrome (NS). Injury of podocyte has been proved to contribute to the occurrence of proteinuria. This study explored the effects of geniposide (GEN) on lipopolysaccharide (LPS)-caused murine kidney podocyte MPC5 apoptosis and autophagy. Viability and apoptosis of MPC5 cells were respectively detected with the help of CCK-8 assay and Guava Nexin assay. 3-Methyladenine (3-MA) was used as an autophagy inhibitor, while rapamycin as autophagy activator. Si-Beclin-1 was transfected in MPC5 cells to down-regulate the expression of Beclin-1. We found that LPS stimulation significantly caused MPC5 cell viability reduction, apoptosis and autophagy (P < .05 or P < .01). GEN treatment remarkably alleviated the LPS-caused MPC5 cell viability reduction and apoptosis, but promoted cell autophagy (P < .05). Moreover, 3-MA incubation or si-Beclin-1 transfection notably weakened the effects of GEN on LPS-caused MPC5 cell apoptosis and autophagy (P < .05), while rapamycin had opposite effects (P < .05). Furthermore, GEN activated Ras/Raf/MEK/ERK pathway in LPS-treated MPC5 cells (P < .05). In conclusion, this research verified the protective effects of GEN on podocytes damage. GEN alleviates LPS-caused apoptosis of murine kidney podocytes by activating Ras/Raf/MEK/ERK-mediated cell autophagy. Highlights: LPS causes podocyte MPC5 apoptosis and autophagy. GEN alleviates LPS-caused MPC5 cell apoptosis, but promotes cell autophagy. 3-MA or si-Beclin-1 weakens the effects of GEN on LPS-treated MPC5 cells. Rapamycin strengthens the effects of GEN on LPS-treated MPC5 cells. GEN activates Ras/Raf/MEK/ERK pathway in LPS-treated MPC5 cells.",
              "Parkinson's disease (PD), the second most common neurodegenerative movement disorder, is characterized by progressive motor and non-motor symptoms [1]. Despite treatment with pharmacologic and surgical therapies, the disease will continue to relentlessly advance. Hence, there is a great deal of interest in potential disease-modifying therapies with the hope that the neurodegenerative process can be slowed or halted. The purpose of this review is to highlight the role toxic alpha-synuclein (alpha-syn) plays in PD pathogenesis and critically review the relevant literature about therapeutic modalities targeting alpha-syn. Toxic alpha-syn plays a key role in PD pathogenesis, disrupting important cellular functions, and, thus, targeting alpha-syn is a reasonable disease-modifying strategy. Current approaches under investigation include decreasing alpha-syn production with RNA interference (RNAi), inhibiting alpha-syn aggregation, promoting intracellular degradation of alpha-syn aggregates (via enhancing autophagy and enhancing lysosomal degradation), and promoting extracellular degradation of alpha-syn via active and passive immunization.",
              "Dysregulation of the epigenome and constitutional epimutation lead to aberrant expression of the genes, which regulate cancer initiation and progression. Histone deacetylases (HDACs), which are highly conserved in yeast to humans, are known to regulate numerous proteins involved in the transcriptional regulation of chromatin structures, apoptosis, autophagy, and mitophagy. In addition, a non-permissive chromatin conformation is created by HDACs, preventing the transcription of the genes encoding the proteins associated with tumorigenesis. Recently, an expanding perspective has been reported from the clinical trials with HDACis (HDAC inhibitors), which has emerged as a determining target for the study of the detailed mechanisms underlying cancer progression. Therefore, the present review focuses on the comprehensive lucubration of post-translational modifications and the molecular mechanisms through which HDACs alter the ambiguities associated with epigenome, with particular insights into the initiation, progression, and regulation of cancer.",
              "Although cytokine-based therapy is a promising tool to control the progression of esophageal cancer, low therapeutic responses largely compromise treatment efficacy through unidentified mechanisms. The goal of our study was to explore the roles of macrophage stimulating 1 (Mst1) and mitophagy in enhancing IL-24-based cytokine therapy in esophageal cancer. Our data demonstrated that IL-24 application promoted cancer death by inducing mitochondrial stress, as manifested by mitochondrial ROS overproduction, mitochondrial potential dissipation, cellular ATP deprivation and mitochondrial death activation. Overexpression of Mst1 enhanced IL-24-mediated mitochondrial damage and further augmented IL-24-induced death in esophageal cancer. Molecular investigations illustrated that the IL-24-activated mitochondrial response is accompanied by activation of mitophagy, a protective mechanism to attenuate mitochondrial damage. However, Mst1 overexpression inhibited mitophagy activity, which was achieved by inactivating the ERK-Mfn2 signaling pathway. The re-activation of mitophagy abolished the cancer-killing effects of Mst1 overexpression on esophageal cancer. Altogether, our data demonstrate that IL-24-related therapeutic resistance is associated with mitophagy activation. Mst1 overexpression inhibits mitophagy activity via suppressing the ERK-Mfn2 pathway, ultimately augmenting IL-24-inducd esophageal cancer death via enhanced mitochondrial stress.",
              "Enhancement of insulin-like growth factor 1 receptor (IGF-IR) degradation by heat shock protein 90 (HSP90) inhibitor is a potential antitumor therapeutic strategy. However, very little is known about how IGF-IR protein levels are degraded by HSP90 inhibitors in pancreatic cancer (PC). We found that the HSP90alpha inhibitor NVP-AUY922 (922) effectively downregulated and destabilized the IGF-1Rbeta protein, substantially reduced the levels of downstream signaling molecules (p-AKT, AKT and p-ERK1/2), and resulted in growth inhibition and apoptosis in IGF-1Rbeta-overexpressing PC cells. Preincubation with a proteasome or lysosome inhibitor (MG132, 3 MA or CQ) mainly led to IGF-1Rbeta degradation via the lysosome degradation pathway, rather than the proteasome-dependent pathway, after PC cells were treated with 922 for 24 h. These results might be associated with the inhibition of pancreatic cellular chymotrypsin-peptidase activity by 922 for 24 h. Interestingly, 922 induced autophagic flux by increasing LC3II expression and puncta formation. However, knockdown of the crucial autophagy component AGT5 and the chemical inhibitor 3 MA-blocked 922-induced autophagy did not abrogate 922-triggered IGF-1Rbeta degradation. Furthermore, 922 could enhance chaperone-mediated autophagy (CMA) activity and promote the association between HSP/HSC70 and IGF-1Rbeta or LAMP2A in coimmunoprecipitation and immunofluorescence analyses. Silencing of LAMP2A to inhibit CMA activity reversed 922-induced IGF-1Rbeta degradation, suggesting that IGF-1Rbeta degradation by 922 was partially dependent on the CMA pathway rather than macroautophagy. This finding is mirrored by the identification of the KFERQ-like motif in IGF-1Rbeta. These observations support the potential application of 922 for IGF-1Rbeta-overexpressing PC therapy and first identify the role of the CMA pathway in IGF-1Rbeta degradation by an HSP90 inhibitor.",
              "Autophagy maintains homeostasis and is induced upon stress. Yet, its mechanistic interaction with oncogenic signaling remains elusive. Here, we show that in BRAF(V600E)-melanoma, autophagy is induced by BRAF inhibitor (BRAFi), as part of a transcriptional program coordinating lysosome biogenesis/function, mediated by the TFEB transcription factor. TFEB is phosphorylated and thus inactivated by BRAF(V600E) via its downstream ERK independently of mTORC1. BRAFi disrupts TFEB phosphorylation, allowing its nuclear translocation, which is synergized by increased phosphorylation/inactivation of the ZKSCAN3 transcriptional repressor by JNK2/p38-MAPK. Blockade of BRAFi-induced transcriptional activation of autophagy-lysosomal function in melanoma xenografts causes enhanced tumor progression, EMT-transdifferentiation, metastatic dissemination, and chemoresistance, which is associated with elevated TGF-beta levels and enhanced TGF-beta signaling. Inhibition of TGF-beta signaling restores tumor differentiation and drug responsiveness in melanoma cells. Thus, the \"BRAF-TFEB-autophagy-lysosome\" axis represents an intrinsic regulatory pathway in BRAF-mutant melanoma, coupling BRAF signaling with TGF-beta signaling to drive tumor progression and chemoresistance.",
              ": Autophagy plays a role in several physiological and pathological processes as it controls the turnover rate of cellular components and influences cellular homeostasis. The liver plays a central role in controlling organisms' metabolism, regulating glucose storage, plasma proteins and bile synthesis and the removal of toxic substances. Liver functions are particularly sensitive to autophagy modulation. In this review we summarize studies investigating how autophagy influences the hepatic metabolism, focusing on fat accumulation and lipids turnover. We also describe how autophagy affects bile production and the scavenger function within the complex homeostasis of the liver. We underline the role of hepatic autophagy in counteracting the metabolic syndrome and the associated cardiovascular risk. Finally, we highlight recent reports demonstrating how the autophagy occurring within the liver may affect skeletal muscle homeostasis as well as different extrahepatic solid tumors, such as melanoma.",
              "The pleiotropic effects of hyperthermia on cancer cells have been well documented, and microwave hyperthermia (MWHT) has been widely applied for multifarious cancer treatment. However, the mechanisms underlying the anticancer effect of MWHT combined with gemcitabine (GEM) remain poorly understood. The aim of the present study was to investigate the role of autophagy in the thermochemotherapy of human squamous cell lung carcinoma cells. It was observed that MWHT combined with GEM potently suppressed the viability of NCIH2170 and NCIH1703 cells, and induced G0/G1 cell cycle arrest. Notably, MWHT with GEM induced autophagy, as indicated by the formation of autophagic vacuoles, downregulation of p62 and upregulation of light chain 3II. It was further demonstrated that the autophagy was due to the production of reactive oxygen species (ROS), whereas Nacetyl cysteine, an ROS scavenger, attenuated the level of autophagy. However, when the autophagy inhibitor 3methyladenine was used, there was no significant change in the production of ROS. Furthermore, it was observed that MWHT combined with GEM downregulated the protein expression levels of phosphoinositide 3kinase (PI3K), phosphorylated (p)PI3K, protein kinase B (AKT), pAKT, mammalian target of rapamycin (mTOR), pmTOR, phosphorylated S6 (pS6) and p70 S6 kinase, which are associated with autophagy. In addition, the results demonstrated that ROS served as an upstream mediator of PI3K/AKT/mTOR signaling. In light of these findings, the present study provides original insights into the molecular mechanisms underlying the cell death induced by MWHT combined with GEM, and this may be a promising approach for the treatment of human squamous cell lung carcinoma.",
              "BACKGROUND: Prostate cancer (PCa) remains a challenge worldwide. Due to the development of castration-resistance, traditional first-line androgen deprivation therapy (ADT) became powerlessness. Epidermal growth factor receptor (EGFR) is a well characterized therapeutic target to treat colorectal carcinoma and non-small cell lung cancer. Increasing studies have unraveled the significance of EGFR and its downstream signaling in the progression of castration-resistant PCa. METHOD: MTS, colony formation and Edu staining assays were used to analyze the cell proliferation of PCa cells. Flow cytometry was used to analyze PCa cell cycle distribution and cell apoptosis. Western blot was used to measure the expression of key proteins associated with cell cycle progression, apoptosis and EGFR signaling pathways. Transfection of exogenous small interfering RNA (siRNA) or plasmid was used to intervene specific gene expression. Nude mouse model was employed to test the in vivo effect of Spautin-1. RESULTS: The current study reveals that Spautin-1, a known inhibitor of ubiquitin-specific peptidase 10 (USP10) and USP13, inhibits EGFR phosphorylation and the activation of its downstream signaling. Inhibition of EGFR signaling induced by Spautin-1 leads to cell cycle arrest and apoptosis of PCa in a USP10/USP13 independent manner. The application of Spautin-1 reduces the expression of glucose transporter 1 (Glut1) and dramatically induces cell death under glucose deprivation condition. In vivo experiments show a potent anti-tumor effect of Spautin-1 alone and in combination with Enzalutamide. CONCLUSION: This study demonstrates the therapeutic potential of EGFR signaling inhibition by the use of Spautin-1 for PCa treatment.",
              "Maintenance of a healthy proteome is essential for cellular homeostasis and loss of proteostasis is associated with tissue dysfunction and neurodegenerative disease. The mechanisms that support proteostasis in healthy cells and how they become defective during aging or in disease states are not fully understood. Here, we investigate the transcriptional programs that are essential for neural stem and progenitor cell (NSPC) function and uncover a program of autophagy genes under the control of the transcription factor FOXO3. Using genomic approaches, we observe that FOXO3 directly binds a network of target genes in adult NSPCs that are involved in autophagy, and find that FOXO3 functionally regulates induction of autophagy in these cells. Interestingly, in the absence of FOXO activity, aggregates accumulate in NSPCs, and this effect is reversed by TOR (target of rapamycin) inhibition. Surprisingly, enhancing FOXO3 causes nucleation of protein aggregates, but does not increase their degradation. The work presented here identifies a genomic network under the direct control of a key transcriptional regulator of aging that is critical for maintaining a healthy mammalian stem cell pool to support lifelong neurogenesis.",
              "Acute pancreatitis (AP) is a potentially lethal inflammatory disease that lacks specific therapy. Damaged pancreatic acinar cells are believed to be the site of AP initiation. The primary function of these cells is the synthesis, storage, and export of digestive enzymes. Beginning in the endoplasmic reticulum and ending with secretion of proteins stored in zymogen granules, distinct pancreatic organelles use ATP produced by mitochondria to move and modify nascent proteins through sequential vesicular compartments. Compartment-specific accessory proteins concentrate cargo and promote vesicular budding, targeting, and fusion. The autophagy-lysosomal-endosomal pathways maintain acinar cell homeostasis by removing damaged/dysfunctional organelles and recycling cell constituents for substrate and energy. Here, we discuss studies in experimental and genetic AP models, primarily from our groups, which show that acinar cell injury is mediated by distinct mechanisms of organelle dysfunction involved in protein synthesis and trafficking, secretion, energy generation, and autophagy. These early AP events (often first manifest by abnormal cytosolic Ca signaling) in the acinar cell trigger the inflammatory and cell death responses of pancreatitis. Manifestations of acinar cell organelle disorders are also prominent in human pancreatitis. Our findings suggest that targeting specific mediators of organelle dysfunction could reduce disease severity.",
              "BACKGROUND: Lung cancer patients with KRAS mutation(s) have a poor prognosis due in part to the development of resistance to currently available therapeutic interventions. Development of a new class of anticancer agents that directly targets KRAS may provide a more attractive option for the treatment of KRAS-mutant lung cancer. RESULTS: Here we identified a small molecule KRAS agonist, KRA-533, that binds the GTP/GDP-binding pocket of KRAS. In vitro GDP/GTP exchange assay reveals that KRA-533 activates KRAS by preventing the cleavage of GTP into GDP, leading to the accumulation of GTP-KRAS, an active form of KRAS. Treatment of human lung cancer cells with KRA-533 resulted in increased KRAS activity and suppression of cell growth. Lung cancer cell lines with KRAS mutation were relatively more sensitive to KRA-533 than cell lines without KRAS mutation. Mutating one of the hydrogen-bonds among the KRA-533 binding amino acids in KRAS (mutant K117A) resulted in failure of KRAS to bind KRA-533. KRA-533 had no effect on the activity of K117A mutant KRAS, suggesting that KRA-533 binding to K117 is required for KRA-533 to enhance KRAS activity. Intriguingly, KRA-533-mediated KRAS activation not only promoted apoptosis but also autophagic cell death. In mutant KRAS lung cancer xenografts and genetically engineered mutant KRAS-driven lung cancer models, KRA-533 suppressed malignant growth without significant toxicity to normal tissues. CONCLUSIONS: The development of this KRAS agonist as a new class of anticancer drug offers a potentially effective strategy for the treatment of lung cancer with KRAS mutation and/or mutant KRAS-driven lung cancer.",
              "Objective:To investigate the expression of microtubuledouble ended arrowassociated protein 1 light chain 3 beta(LC3) and eosinophil cationic protein in allergic rhinitis(AR) for further understanding of the pathogenesis of AR. Method: Twenty cases of normal nasal mucosa and 20 cases of AR nasal mucosa were collected. Histological changes of nasal mucosa were examined by hematoxylin and eosin(HE) staining. The expression of LC3 and ECP were measured by immunohistochemistry(IHC) and Western Blot(WB). Result: The tissue samples demonstrated a large number of eosinophils and lymphocytes infiltration in AR. IHC revealed that LC3 and ECP expression were higher in AR than in normal nasal mucosa(P<0.05). WB also showed that the relative expression levels of protein expression of LC3 and ECP were greater in AR than in controls. The expression level of LC3 was positively correlated with that of ECP protein in AR. Conclusion: LC3 and ECP were upregulated and positively correlated in AR, indicating that autophagy plays an important role in the toxicity of allergic rhinitis , which provides theoretical basis for the precise treatment of AR.",
              "AIMS: Osteoarthritis (OA) is a chronic and degenerative joint disease prevalent in the elderly, which is characterized by hypertrophy and reactive hyperplasia of articular cartilage. Autophagy has been reported to inhibit inflammation and reduce chondrocyte apoptosis in OA. As the microRNA (miRNA)-335-5p has been linked to both inflammation and autophagy, this study aimed to investigate its potential role in regulating autophagy during the pathogenesis of OA. MAIN METHODS: Quantitative real-time PCR (qRT-PCR) was used to detect miRNA-335-5p expression in normal and OA human chondrocytes. Following transfection of human OA chondrocytes with double-stranded miRNA-335-5p mimic/inhibitor, qRT-PCR, western blotting, and immunofluorescence were used to determine expression levels of the inflammatory mediators IL-1beta, IL-6, and TNF-alpha, and the autophagic markers Beclin-1, autophagy-related protein 5 (ATG5), and ATG7. The autophagy inhibitor 3-methyladenine (3-MA) was used to link the anti-inflammatory effects of miRNA-335-5p to autophagy. KEY FINDINGS: The expression of miRNA-335-5p was significantly lower in OA chondrocytes than in normal chondrocytes. Transfection of human OA chondrocytes with the miRNA-335-5p mimic led to a remarkable increase in viability, a significant increase in autophagy-related factors, and a reduction in inflammatory mediators. Importantly, treatment of miRNA-335-5p-overexpressing OA chondrocytes with the autophagy inhibitor 3-MA restored the expression of inflammatory mediators. SIGNIFICANCE: We conclude that miRNA-335-5p can significantly alleviate inflammation in human OA chondrocytes by activating autophagy. Therefore, miRNA-335-5p has potential for future use in the clinical diagnosis and treatment of OA.",
              "Emerging evidence shows that palmitic acid (PA), a common fatty acid in the human diet, serves as a signaling molecule regulating the progression and development of many diseases at the molecular level. In this review, we focus on its regulatory roles in the development of five pathological conditions, namely, metabolic syndrome, cardiovascular diseases, cancer, neurodegenerative diseases, and inflammation. We summarize the clinical and epidemiological studies; and also the mechanistic studies which have identified the molecular targets for PA in these pathological conditions. Activation or inactivation of these molecular targets by PA controls disease development. Therefore, identifying the specific targets and signaling pathways that are regulated by PA can give us a better understanding of how these diseases develop for the design of effective targeted therapeutics.",
              "The major neurological feature of prion diseases is a neuronal loss accomplished through either apoptosis or autophagy. In this review, I compared axonal alterations in prion diseases to those described 40 years earlier as a result of nerve ligation. I also demonstrated that autophagic vacuoles and autophagosomes are a major part of dystrophic neurites. Furthermore, I summarized the current status of the autophagy in prion diseases and hypothesize, that spongiform change may originate from the autophagic vacuoles. This conclusion should be supported by other methods, in particular laser confocal microscopy. We observed neuronal autophagic vacuoles in different stages of formation, and our interpretation of the 'maturity' of their formation may or may not equate to actual developmental stages. Initially, a part of the neuronal cytoplasm was sequestrated within double or multiple membranes (phagophores) and often exhibited increased electron-density. The intracytoplasmic membranes formed labyrinth-like structures that suggest a multiplication of those membranes. The autophagic vacuoles then expand and eventually, a vast area of the cytoplasm was transformed into a merging mass of autophagic vacuoles. Margaret R. Matthews published a long treatise in the Philosophical Transactions of the Royal Society of London in which she had described in great detail the ultrastructure of postganglionic branches of the superior cervical ganglion in the rat following ligation of them. The earliest changes observed by Matthews between 6 h to 2 days in the proximal stump were distensions of proximal axons. Analogously, in our models, an increased number of 'regular' (round) and 'irregular' MVB and some autophagic vacuoles were observed collectively, both processes were similar.",
              "beta-Elemene is a sesquiterpene compound extracted from the herb Curcuma Rhizoma and is used in traditional Chinese medicine (TCM) to treat several types of cancer, with no reported severe adverse effects. Recent studies, using in vitro and in vivo studies combined with molecular methods, have shown that beta-elemene can inhibit cell proliferation, arrest the cell cycle, and induce cell apoptosis. Recent studies have identified the molecular targets of beta-elemene that may have a role in cancer therapy. This review aims to discuss the anticancer potential of beta-elemene through its actions on several molecular targets including kinase enzymes, transcription factors, growth factors and their receptors, and proteins. beta-Elemene also regulates the expression of several key molecules that are involved in tumor angiogenesis and metastasis including vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMPs), E-cadherin, N-cadherin, and vimentin. Also, beta-elemene has been shown to have regulatory effects on the immune response and increases the sensitivity of cancer cells to chemoradiotherapy and has shown effects on multidrug resistance (MDR) in malignancy. Recent studies have shown that beta-elemene can induce autophagy, which prevents cancer cells from undergoing apoptosis. Therefore, the molecular mechanisms for the treatment effects on cancer of the herbal extract, beta-elemene, which has been used for centuries in traditional Chinese medicine, are now being studied and identified.",
              "Lead (Pb) is an environmental pollutant and has toxic effect on birds. Selenium (Se) has alleviative effect on Pb poisoning. This study investigated mitigative effect of Se on autophagy in Pb-treated chicken testes. Seven-day-old male chickens were randomly divided into four groups with 45 birds in each group. The birds of the control group were offered drinking water (DW) and commercial diet (CD) (0.49mg/kg Se). The birds of the Se group were offered DW and CD containing sodium selenite (SeCD) (1.00mg/kg Se). The birds of the Pb group were offered DW containing lead acetate (PbDW) (350.00mg/L Pb) and CD. The birds of the Pb/Se group were offered PbDW and SeCD. On the 30(th), 60(th), and 90(th) days, respectively; histology, antioxidant indexes (hydrogen peroxide (H2O2), catalase (CAT), total antioxidant capacity (TAOC), reduced glutathione (GSH), and superoxide dismutase (SOD)), mRNA and protein levels of autophagy-related genes (autophagy-related proteins 5, Beclin 1, Dynein, light chain 3 (LC3)-I, LC3-II, and mammalian target of rapamycin (mTOR)) were performed in chicken testes. The results of this study showed that Pb caused histological changes; increased H2O2 content; decreased CAT, TAOC, and SOD activities and GSH content; and increased mRNA and protein levels of the above autophagy-related genes except that mTOR decreased in chicken testes. Se alleviated the above changes. Se alleviated histological damage, oxidative stress, and autophagy in the Pb-treated chicken testes.",
              "Multiple drug resistance (MDR) often occurs after prolonged chemotherapy, leading to refractory tumor and cancer recurrence. Autophagy as a primarily process during starvation or stress has a bipolar nature in cancer. It can cause MDR to become more difficult or make resistant cancer cells more susceptible to chemotherapeutic agents. A number of natural products have been introduced to drug discovery for many years. Some of these compounds have been shown to reverse drug resistance by different regulatory mechanisms. In this review, the focus is on the role of medicinal plants in the MDR phenomenon, primarily through the autophagy process.",
              "Objective: The objective of this study was to investigate the molecular mechanisms involved in rapamycin-induced inhibition of tumor growth. Materials and Methods: Murine S180 sarcoma cells were subcutaneously injected into mice, and the tumor-bearing mice were randomly divided into three groups (vehicle control, 2 mg/kg rapamycin, and 4 mg/kg rapamycin). The effect of rapamycin on tumor growth was determined by measuring tumor volume. Mammalian target of rapamycin (mTOR), Beclin1, ULK1, LC3, Notch1, CD133, and CD90 expressions was confirmed using confocal microscopy and Western blotting. Results: The tumor growth inhibition rates induced by high-dose and low-dose rapamycin were 48.8% and 30.1%, respectively. Beclin1 and ULK1 expressions and the LC3-II/LC3-I ratio in tumor tissues were altered by rapamycin, whereas mTOR, Notch1, CD133, and CD90 expressions were significantly inhibited by rapamycin in immunofluorescence assays. Western blotting also showed similar results. Conclusion: Tumor growth delay induced by rapamycin may be associated with the suppression of the cancer stem cell phenotype (Notch1, CD133, and CD90) and promotion of autophagy (mTOR, Beclin1, ULK1, and LC3-II/LC3-I ratio) in the murine S180 sarcoma model.",
              "Background: The receptor for advanced glycation end-product (RAGE) was one of the signal transduction receptors. RAGE interacted with various signaling molecules which were involved in human disease processes including tumorigenesis. Previous reports have indicated that RAGE/high-mobility group box 1 (HMGB1) could regulate autophagy in different carcinomas. However, the functional role of RAGE/ HMGB1 in the regulation of clear cell renal cell carcinoma (ccRCC) autophagy remained unrevealed. Methods: Western blot, quantitative real-time polymerase chain reaction (qRT-PCR) and immunofluorescence were used in the present study. Results: In this study, we demonstrated that the levels of RAGE/HMGB1 and autophagic protein LC3, Beclin-1, PI3K were much higher in ccRCC samples than those of in adjacent normal tissues. RAGE and autophagic protein expression was regulated with RAGE/HMGB1 in human RCC cell lines. Conclusion: Our results implicated that RAGE and autophagy played important roles in ccRCC, and RAGE/HMGB1 might serve as a novel therapeutic target for future ccRCC treatment.",
              "Green tea is one of the most beverages with antioxidants and nutrients. As one of the major components of green tea, (-)-epicatechin gallate (ECG) was evaluated for its antioxidative properties in the present study. Cell proliferation assay, tube formation, cell migration, apoptosis, and autophagy were performed in human brain microvascular endothelial cells (HBMVECs) after oxygen-glucose deprivation/reoxygenation (OGD/R) to investigate potential anti-ischemia/reperfusion injury properties of ECG in vitro. Markers of oxidative stress as ROS, LDH, MDA, and SOD were further assayed in our study. Data indicated that ECG could affect neovascularization and promote cell proliferation, tube formation, and cell migration while inhibiting apoptosis and autophagy through affecting VEGF, Bcl-2, BAX, LC3B, caspase 3, mTOR, and Beclin-1 expression. All the data suggested that ECG may be protective for the brain against ischemia/reperfusion injury by promoting neovascularization, alleviating apoptosis and autophagy, and promoting cell proliferation in HBMVECs of OGD/R.",
              "Autophagy allows for lysosomal cellular degradation of cytosolic components. In particular, neuronal autophagy is essential for cellular homeostasis and neuronal survival and is tightly regulated by several autophagy-related (ATG) proteins in post-mitotic neurons. Among these ATG proteins, the LC3/GABARAP proteins are known to regulate autophagosome biogenesis/maturation and cargo recognition. However, little is known about the role of GABARAP family proteins in neuronal autophagy despite their abundant expression in post-mitotic neurons. We have previously developed HyD (Hydrophobic Domain)-LIR (LC3-interacting region)-based autophagosome markers. In this study, to monitor GABARAP family proteins in autophagosomes of post-mitotic neurons, we improved the sensitivity of the probes for specifically detecting endogenous GABARAP family proteins by adding one more LIR motif to the LIR probes. We have tested the efficiency of two different LIRs, from ULK2 and Stbd1, in regard to their cellular localization to autophagosomes. HyD-2xLIR(ULK2)-GFP and HyD-2xLIR(Stbd1)-GFP demonstrated specific localization to GABARAP-positive autophagosomes relative to LC3B-positive autophagosomes in MEF/HeLa cells in an autophagy-dependent manner. Indeed, HyD-2xLIR(Stbd1)-GFP could efficiently detect GABARAP-positive autophagosomes in cultured cortical neurons. Our improved GABARAP-sensitive probes will contribute toward understanding the specific role of GABARAP family proteins in regard to neuronal autophagy.",
              "Cathepsin D is one of the major lysosomal aspartic proteases that is essential for the normal functioning of the autophagy-lysosomal system. In the kidney, cathepsin D is enriched in renal proximal tubular epithelial cells, and its levels increase during acute kidney injury. To investigate how cathepsin D-deficiency impacts renal proximal tubular cells, we employed a conditional knockout CtsD(flox/-); Spink3(Cre) mouse. Immunohistochemical analyses using anti-cathepsin D antibody revealed that cathepsin D was significantly decreased in tubular epithelial cells of the cortico-medullary region, mainly in renal proximal tubular cells of this mouse. Cathepsin D-deficient renal proximal tubular cells showed an increase of microtubule-associated protein light chain 3 (LC3; a marker for autophagosome/autolysosome)-signals and an accumulation of abnormal autophagic structures. Renal ischemia/reperfusion injury resulted in an increase of early kidney injury marker, Kidney injury molecule 1 (Kim-1), in the cathepsin D-deficient renal tubular epithelial cells of the CtsD(flox/-); Spink3(Cre) mouse. Inflammation marker was also increased in the cortico-medullary region of the CtsD(flox/-); Spink3(Cre) mouse. Our results indicated that lack of cathepsin D in the renal tubular epithelial cells led to an increase of sensitivity against ischemia/reperfusion injury.",
              "Chronic arsenic exposure through water intake is a worldwide issue, which has caused many diseases. Lungs are the first target organ of arsenic and lung inflammation, autophagy, and even the onset of tumors can be induced by arsenic exposure. Here, we tested the outcome of low-concentration arsenic exposure in rat lungs. Tissue changes, inflammation, autophagy, and other physiological responses were observed in this study. Results showed that low-concentration exposure of arsenite through water intake could initiate autophagy and inflammation in lungs but high concentration exposure produced a weak autophagy response and accentuated inflammation with the possibility of a chronic inflammation environment emerging followed by tumorigenesis.",
              "Abamectin (ABA) is one of the most widely used compounds in agriculture and veterinary medicine. However, the cytotoxicity of ABA in human gastric cells is utterly unknown. In this study, ABA suppressed the proliferation of MGC803 cells by arresting the cell cycle at the G0/G1-phase. Moreover, ABA induced mitochondrial-mediated apoptosis by inducing the loss of mitochondrial membrane potential, upregulation of Bax/Bcl-2, and activation of caspase-3. ABA significantly improved the LC3-II/LC3-I ratio and reduced P62 protein expression in a dose-dependent manner. Through detection of the reactive oxygen species (ROS) levels, we found ABA induced the accumulation of intracellular ROS and then reduced PI3K/AKT signaling activation related to MGC803 cell apoptosis and autophagy. Our results indicate that ABA exerts cytotoxic effects on human MGC803 cells through apoptosis and autophagy by inhibiting ROS-mediated PI3K/AKT signaling. Furthermore, ABA may be a potential risk to human gastric health.",
              "Cell death was once believed to be the result of one of two distinct processes, apoptosis (also known as programmed cell death) or necrosis (uncontrolled cell death); in recent years, however, several other forms of cell death have been discovered highlighting that a cell can die via a number of differing pathways. Apoptosis is characterised by a number of characteristic morphological changes in the structure of the cell, together with a number of enzyme-dependent biochemical processes. The result being the clearance of cells from the body, with minimal damage to surrounding tissues. Necrosis, however, is generally characterised to be the uncontrolled death of the cell, usually following a severe insult, resulting in spillage of the contents of the cell into surrounding tissues and subsequent damage thereof. Failure of apoptosis and the resultant accumulation of damaged cells in the body can result in various forms of cancer. An understanding of the pathways is therefore important in developing efficient chemotherapeutics. It has recently become clear that there exists a number of subtypes of apoptosis and that there is an overlap between apoptosis, necrosis and autophagy. The goal of this review is to provide a general overview of the current knowledge relating to the various forms of cell death, including apoptosis, necrosis, oncosis, pyroptosis and autophagy. This will provide researchers with a summary of the major forms of cell death and allow them to compare and contrast between them.",
              "Interferons (IFNs) have anti-viral and anti-tumour effects. Type III interferon, as a member of the recently discovered interferon family, has been proved to inhibit tumour proliferation and promote the apoptosis of various tumour cells. However, whether type III IFN could inhibit the proliferation of lung cancer was not clear. In this study, we found that interferon lambda (IFN lambda) could inhibit the proliferation of A549 cells and induce autophagy and apoptosis of A549 cells. IFN lambda could promote the expression of autophagy gene Beclin1 and interfere the expression of autophagy gene Beclin1 with small interfering RNA, thus inhibiting the effect of type III interferon on anti-proliferation and promoting apoptosis of lung cancer cell. These results suggested that IFN lambda could inhibit the proliferation of A549 cells by activating autophagy pathway, and IFN lambda might be one of the potential therapeutic drugs for lung cancer.",
              "Circular RNAs (circRNAs) play critical roles in various diseases. However, whether and how circular RNA regulates influenza A virus (IAV) infection is unknown. Here, we studied the role of circular RNA GATA Zinc Finger Domain Containing 2A (circ-GATAD2A) in the replication of IAV H1N1 in A549 cells. Circ-GATAD2A was formed upon H1N1 infection. Knockdown of circ-GATAD2A in A549 cells enhanced autophagy and inhibited H1N1 replication. By contrast, overexpression of circ-GATAD2A impaired autophagy and promoted H1N1 replication. Similarly, knockout of vacuolar protein sorting 34 (VPS34) blocked autophagy and increased H1N1 replication. However, the expression of circ-GATAD2A could not further enhance H1N1 replication in VPS34 knockout cells. Collectively, these data indicated that circ-GATAD2A promotes the replication of H1N1 by inhibiting autophagy.",
              "This study aimed to explore mechanisms of the effects of hydroxysafflor yellow A (HSYA) on neural stem cells (NSCs) after heat stress (HS). Rat NSCs cells were cultured at 42 degrees C to impose heat stress. Cell counting kit-8 and Edu assay were used to analyze NSC proliferation. Annexin V/PI apoptosis kit was used to detect NSC apoptosis. Expression and phosphorylation of autophagy and apoptosis-associated proteins were determined by western blotting. We showed that HSYA significantly promoted proliferation and attenuated apoptosis of NSCs after heat stress. HSYA also increased Bcl-2 expression but decreased the expression of Bax and cleaved caspase-3 in NSCs induced by heat stress. In addition, HSYA decreased p38 and Hsp27-78 phosphorylation and MK-2 expression after heat stress, which was consistent with NSCs treated with SB203850 treatment or p38 knockdown. Furthermore, we demonstrated that heat stress increased LC3-II expression and mTOR phosphorylation, and decreased the expression of p62 in NSCs, while HSYA, SB203850 treatment or p38 knockdown reversed these alterations. In conclusion, HSYA significantly reversed the apoptosis and autophagy of NSCs induced by heat stress (P < 0.05), via downregulating MK2 expression and p38 and Hsp27-78 phosphorylation.",
              "AIMS: In myocardial ischemia-reperfusion (MI/R) injury, impaired autophagy function worsens cardiomyocyte death. AMP-activated protein kinase (AMPK) is a heterotrimeric protein that plays an important role in cardioprotection and myocardial autophagic function. AMPKalpha1 and alpha2 are localized primarily in the cytoplasm and nucleus, respectively, in cardiomyocytes, but the isoform-specific autophagy regulation of AMPK during MI/R remains unclear. MATERIALS AND METHODS: An MI/R model was built, and the protein expression of AMPKalpha1/alpha2, p-AMPK, mTOR, p-mTOR, TFEB, p-FoxO3a, SKP2, CARM1, TBP, Atg5, LAMP2, LC3B, and p62 during ischemia and reperfusion was determined by western blotting. Recombinant adeno-associated virus (serotype 9) vectors carrying tandem fluorescent-tagged LC3 or mRFP-GFP-LC3/GFP-LC3 were used to evaluate the autophagy status. AMPKalpha2 knockout mice were used for in vivo studies. KEY FINDINGS: Both cytoplasmic AMPKalpha1 and nuclear alpha2 subunit expression decreased during the reperfusion period, which led to AMPKalpha1-mTOR-TFEB and AMPKalpha2-Skp2-CARM1-TFEB signaling inhibition, respectively. The decreased TFEB level during reperfusion suppressed autophagy. Metformin could activate both the AMPKalpha1- and alpha2- mediated pathways, thus restoring autophagy flux during reperfusion. Nevertheless, in AMPKalpha2 knockout mice, nuclear alpha2-regulated Skp2-CARM1-TFEB signaling was inhibited, while alpha1-related signaling was comparatively unaffected, which partially impaired metformin-enhanced autophagy. SIGNIFICANCE: Our study suggests that metformin had the dual effects of promoting both cytoplasmic AMPKalpha1- and nuclear AMPKalpha2-related signaling to improve autophagic flux and restore cardiac function during MI/R.",
              "BACKGROUND: Gallbladder cancer is the most common biliary tract malignancy and not sensitive to chemotherapy. Autophagy is an important factor prolonging the survival of cancer cells under chemotherapeutic stress. We aimed to investigate the role of long non-coding RNAs (lncRNAs) in autophagy and chemoresistance of gallbladder cancer cells. METHODS: We established doxorubicin (Dox)-resistant gallbladder cancer cells and used microarray analysis to compare the expression profiles of lncRNAs in Dox-resistant gallbladder cancer cells and their parental cells. Knockdown or exogenous expression of lncRNA combined with in vitro and in vivo assays were performed to prove the functional significance of lncRNA. The effects of lncRNA on autophagy were assessed by stubRFP-sensGFP-LC3 and western blot. We used RNA pull-down and mass spectrometry analysis to identify the target proteins of lncRNA. RESULTS: The drug-resistant property of gallbladder cancer cells is related to their enhanced autophagic activity. And we found a lncRNA ENST00000425894 termed gallbladder cancer drug resistance-associated lncRNA1 (GBCDRlnc1) that serves as a critical regulator in gallbladder cancer chemoresistance. Furthermore, we discovered that GBCDRlnc1 is upregulated in gallbladder cancer tissues. Knockdown of GBCDRlnc1, via inhibiting autophagy at initial stage, enhanced the sensitivity of Dox-resistant gallbladder cancer cells to Dox in vitro and in vivo. Mechanically, we identified that GBCDRlnc1 interacts with phosphoglycerate kinase 1 and inhibits its ubiquitination in Dox-resistant gallbladder cancer cells, which leads to the down-regulation of autophagy initiator ATG5-ATG12 conjugate. CONCLUSIONS: Our findings established that the chemoresistant driver GBCDRlnc1 might be a candidate therapeutic target for the treatment of advanced gallbladder cancer.",
              "The class 3 phosphoinositide 3-kinase (PI3K) is required for lysosomal degradation by autophagy and vesicular trafficking, assuring nutrient availability. Mitochondrial lipid catabolism is another energy source. Autophagy and mitochondrial metabolism are transcriptionally controlled by nutrient sensing nuclear receptors. However, the class 3 PI3K contribution to this regulation is unknown. We show that liver-specific inactivation of Vps15, the essential regulatory subunit of the class 3 PI3K, elicits mitochondrial depletion and failure to oxidize fatty acids. Mechanistically, transcriptional activity of Peroxisome Proliferator Activated Receptor alpha (PPARalpha), a nuclear receptor orchestrating lipid catabolism, is blunted in Vps15-deficient livers. We find PPARalpha repressors Histone Deacetylase 3 (Hdac3) and Nuclear receptor co-repressor 1 (NCoR1) accumulated in Vps15-deficient livers due to defective autophagy. Activation of PPARalpha or inhibition of Hdac3 restored mitochondrial biogenesis and lipid oxidation in Vps15-deficient hepatocytes. These findings reveal roles for the class 3 PI3K and autophagy in transcriptional coordination of mitochondrial metabolism.",
              "Selective autophagy ensures the removal of specific soluble proteins, protein aggregates, damaged mitochondria, and invasive bacteria from cells. Defective autophagy has been directly linked to metabolic disorders. However how selective autophagy regulates metabolism remains largely uncharacterized. Here we show that a deficiency in selective autophagy is associated with suppression of lipid oxidation. Hepatic loss of Atg7 or Atg5 significantly impairs the production of ketone bodies upon fasting, due to decreased expression of enzymes involved in beta-oxidation following suppression of transactivation by PPARalpha. Mechanistically, nuclear receptor co-repressor 1 (NCoR1), which interacts with PPARalpha to suppress its transactivation, binds to the autophagosomal GABARAP family proteins and is degraded by autophagy. Consequently, loss of autophagy causes accumulation of NCoR1, suppressing PPARalpha activity and resulting in impaired lipid oxidation. These results suggest that autophagy contributes to PPARalpha activation upon fasting by promoting degradation of NCoR1 and thus regulates beta-oxidation and ketone bodies production.",
              "AIMS: Hydrogen gas (H2) has a diversity of effects such as anti-apoptotic, anti-inflammatory and anti-oxidative properties. However, molecular mechanism underlying the potential effect of H2 on chronic intermittent hypoxia (CIH) induced renal injury remains obscure. MATERIALS AND METHODS: In the present study, adult male Sprague-Dawley rats were randomly allocated into four groups: control (CON) group, CIH group, CIH with H2 treatment (CIH+H2) group, and control with H2 treatment (CON+H2) group. Oxidative stress, autophagy and endoplasmic reticulum (ER) stress were detected to determine how H2 affected the renal function of CIH exposed rats. KEY FINDINGS: We demonstrated that rats who inhale hydrogen gas showed improved renal function, alleviated pathological damage, oxidative stress and apoptosis in CIH rats. Meanwhile, CIH-induced endoplasmic reticulum stress was decreased by H2 as the expressions of CHOP, caspase-12, and GRP78 were down-regulated. Furthermore, relative higher levels of LC3-II/I ratio and Beclin-1, with decreased expression of p62, were found after H2 administrated. Inhibition of mTOR may be involved in the upregulation of autophagy by H2. Finally, increased phosphorylation of p38 and JNK was involved in the CIH-induced pathological process. H2 could inhibit the activation of p38 and JNK, suggesting H2 played an active part in resisting renal injury via MAPK. SIGNIFICANCE: Taken together, our study reveals that H2 can ameliorate CIH-induced kidney injury by decreasing endoplasmic reticulum stress and activating autophagy through inhibiting oxidative stress-dependent p38 and JNK MAPK activation.",
              "Several molecular mechanisms are involved in the regulation of skeletal muscle function. Among them, mitochondrial activity can be identified. The mitochondria is an important and essential organelle in the skeletal muscle that is involved in metabolic regulation and ATP production, which are two key elements of muscle contractibility and plasticity. Thus, in this review, we present the critical and recent antecedents regarding the mechanisms through which mitochondrial dysfunction can be involved in the generation and development of skeletal muscle pathologies, its contribution to detrimental functioning in skeletal muscle and its crosstalk with other typical signaling pathways related to muscle diseases. In addition, an update on the development of new strategies with therapeutic potential to inhibit the deleterious impact of mitochondrial dysfunction in skeletal muscle is discussed.",
              "Mitochondrial dysfunction is a major contributory factor for myocardial ischemia-reperfusion (I/R) injury. It has been reported that Pink1-Parkin-mediated mitochondrial autophagy could effectively remove damaged mitochondria and excess ROS to ensure the stability of intracellular mitochondria. The present study was designed to evaluate whether the polymerized porcine haemoglobin (pPolyHb), a novel type of haemoglobin oxygen carrier, has an effect on I/R injury via regulating the Pink1-Parkin mediated mitochondrial autophagy pathway in myocardial H9C2 cells. The results revealed that pPolyHb could effectively reduce apoptosis and improve the survival rates of H9C2 cells. In addition, Pink1 and Parkin levels gradually decreased with pPolyHb reoxygenation. The inhibition of mitochondrial autophagy through mitochondrial-division inhibitor-1(mdivi-1) resulted in a decrease in anti-apoptotic protein Bcl-2 and an increase in pro-apoptotic protein Bax and CytC. In conclusion, pPolyHb has a protective effect on myocardial ischemia-reperfusion injury by regulating moderate mitochondrial autophagy.",
              "OBJECTIVE: To investigate the effect of electroacupuncture (EA) on cardiac hypertrophy and expression of myocardial autophagy-specific proteins in spontaneously hypertensive rats (SHRs). METHODS: Twelve male Wistar Kyoto (WKY) rats were selected as the normal control group, and 24 male SHRs were randomized into model and EA groups (n12 in each). EA (2 Hz /15 Hz, 1 mA) was applied to \"Taichong\" (LR3) and \"Baihui\" (GV20) for 20 min, once a day for successive 30 days. The other two groups only received the same grasping and fixation procedures. The systolic blood pressure (SBP) was measured using a non-invasive blood detector, and the left ventricular function including the left ventricular anterior wall diameter (LVAWd), left ventricular posterior wall diameter (LVPWd) and left ventricular internal diameter at end-diastolic dimension (LVIDd) was detected using a real-time echocardiography imaging system, and the left ventricular mass index (left ventricle weight / body weight, LVMI) was calculated. The expression of Beclin-1 and LC3 proteins in the left ventricle tissue was detected by Western blot. Pathological changes of the myocardial tissue were observed by Masson staining and transmission electron microscope (TEM). RESULTS: After the intervention, the elevated SBP was significantly lowered in the EA group relevant to the model group (P<0.01) and the increased LVAWd, LVPWd and LVMI were markedly decreased in the EA group (P<0.01, P<0.05), while the reduced LVIDd was significantly ameliorated in the EA group (P<0.01). Masson staining showed that in SHRs, disorder of arrangement of cardiac myofibers, swelling of cardiomyocytes, widened space among myocardial cells with a large number of collagenous fibers were observed, and under TEM, aggregation and unequal size of myocardial mitochondria with vacuolation and rupture, autophagosomes and autophagic lysosomes were seen. These changes were relatively milder in the EA group. Western blot showed that hypertension induced up-regulation of Beclin-1 and LC3-II and ratio of LC3-II/LC3-I, and down-regulation of LC3-I were significantly suppressed in the EA group (P<0.01, P<0.05). CONCLUSION: EA at LR3 and GV20 may improve the left cardiac function, myocardial morphological changes and the degree of autophagy in SHRs, which is related to its effects in down-regulating the expression of myocardial Beclin-1 and LC3-II and in up-regulating expression of LC3-I.",
              "Tetrandrine is a bisbenzylisoquinoline alkaloid known to exhibit anticancer activity against different cancers. In the present study, the cytotoxic effect of tetrandrine isolated from Cyclea peltata on pancreatic (PANC-1) and breast (MDA-MB-231) cancer cells was evaluated in vitro with an attempt to understand the role of tetrandrine on the generation of reactive oxygen species (ROS) and caspase activation. Results demonstrate the dose- and time-dependant cytotoxic effect of tetradrine on both MDA-MB-231 and PANC-1 cells with IC50 values ranging between 51 and 54 muM and 22 and 27 muM for 24 h and 48 h of incubation respectively. In addition, treatment of MDA-MB-231 and PANC-1 cells with tetrandrine showed the shrunken cytoplasm and damaged cell membrane in a dose- and time-dependant manner under the microscope. Also, tetrandrine treatment revealed an elevated levels of reactive oxygen species and increased activities of caspase-8, -9 and -3 confirming the apoptosis of cells through both extrinsic death receptor and intrinsic caspase activation. Therefore, the present study suggests the apoptosis of cells with the activation of caspase pathways mainly intrinsic pathway as a downstream event of tetrandrine-induced ROS generation. Hence, reactive oxygen species-mediated caspase activation pathway may be potentially targeted with the use of tetrandrine to treat breast and pancreatic cancers.",
              "Head and neck cancer is the sixth leading cancer by incidence worldwide. Unfortunately, drug resistance and relapse are the principal limitations of clinical oncology for many patients, and the failure of conventional treatments is an extremely demoralizing experience. It is therefore crucial to find new therapeutic targets and drugs to enhance the cytotoxic effects of conventional treatments without potentiating or offsetting the adverse effects. Melatonin has oncostatic effects, although the mechanisms involved and doses required remain unclear. The purpose of this study is to determine the precise underlying mitochondrial mechanisms of melatonin, which increase the cytotoxicity of oncological treatments, and also to propose new melatonin treatments in order to alleviate and reverse radio- and chemoresistant processes. We analyzed the effects of melatonin on head and neck squamous cell carcinoma (HNSCC) cell lines (Cal-27 and SCC-9), which were treated with 0.1, 0.5, 1, and 1.5 mM melatonin combined with 8 Gy irradiation or 10 muM cisplatin. Clonogenic and MTT assays, as well as autophagy and apoptosis, involving flow cytometry and western blot, were performed in order to determine the cytotoxic effects of the treatments. Mitochondrial function was evaluated by measuring mitochondrial respiration, mtDNA content (RT-PCR), and mitochondrial mass (NAO). ROS production, antioxidant enzyme activity, and GSH/GSSG levels were analyzed using a fluorometric method. We show that high concentrations of melatonin potentiate the cytotoxic effects of radiotherapy and CDDP in HNSCC, which are associated with increased mitochondrial function in these cells. In HNSCC, melatonin induces intracellular ROS, whose accumulation plays an upstream role in mitochondria-mediated apoptosis and autophagy. Our findings indicate that melatonin, at high concentrations, combined with cisplatin and radiotherapy to improve its effectiveness, is a potential adjuvant agent.",
              "Plant sterols (phytosterols) have been widely accepted as a natural anti-cancer agent in multiple malignant tumors. This study was designed to investigate the functions of daucosterol in prostate cancer progression and its possible molecular mechanisms. Our results showed that daucosterol inhibited cell proliferation and induced cell cycle arrest. Moreover, daucosterol treatment obviously promoted apoptosis and autophagy. An autophagy inhibitor, 3-methyladenine (3-MA) was proved to counteract daucosterol-triggered autophagy, growth inhibition, and apoptosis, indicating that daucosterol-induced apoptotic response was dependent on autophagy. Additionally, treatment with daucosterol resulted in increased phosphorylation of c-Jun N-terminal kinase (JNK). Furthermore, pre-treatment with a JNK-specific inhibitor SP600125 abated daucosterol-elicited autophagy and apoptotic cell death. Taken together, our findings demonstrated that daucosterol blocked prostate cancer growth at least partly through inducing autophagic-dependent apoptosis via activating JNK signaling, providing a promising candidate for the development of antitumor drugs in prostate cancer treatment.",
              "Temozolomide (TMZ) is the first choice chemotherapy agent against glioblastoma, but the TMZ chemotherapy resistance has restricted the clinical application. Although autophagy is considered an adaptive response for cell survival under the pressure of chemotherapy and associated with chemotherapy resistance, its initiator and the precise molecular mechanism remains unknown. In the present study, it was determined that TMZ increases the transient receptor potential cation channel subfamily C member 5 (TRPC5) protein expression and the basal autophagy level, and the upregulation of autophagy is mediated by TRPC5 in glioma cells. Additionally, knockdown of TRPC5 upregulated the chemotherapy sensitivity in vitro and in vivo. Furthermore, TRPC5small interfering RNA and pharmacological inhibition indicated that the Ca2+/calmodulin dependent protein kinase beta (CaMKKbeta)/AMPactivated protein kinase alpha (AMPKalpha)/mechanistic target of rapamycin kinase (mTOR) pathway mediates cell survival autophagy during TMZ treatment. In addition, TMZresistant U87/TMZ cells retained a high basal autophagy level, while silence of TRPC5 expression or inhibition of autophagy reversed TMZ resistance. Thus, the present study revealed that TRPC5, an initiator of autophagy, upregulated TMZ resistance via the CaMKKbeta/AMPKalpha/mTOR pathway and this indicated a novel therapeutic site for drug resistance in glioma chemotherapy.",
              "PURPOSE: The prime objective of the present study was to investigate the anticancer properties of angustifoline against COLO-205 human colon cancer cells. Its effects on cell autophagy, apoptosis, cell invasion and cell migration, and cell cycle arrest were also evaluated in the current study. METHODS: WST-1 assay was used to study cytotoxic effects of the compound on the cell viability. Effects on apoptosis and cell cycle arrest were evaluated by flow cytometry. In vitro wound healing assay and matrigel assay were carried out to study the effects of angustifoline on cell migration and cell invasion respectively. To confirm autophagy, we evaluated the expression of several autophagy-associated proteins using Western blot assay along with transmission electron microscopy (TEM). RESULTS: The findings indicated that angustifoline induced dose- and time-dependent cytotoxicity in COLO-205 human colon cancer cells along with inhibiting cancer cell colony formation. Angustifoline-treated cells exhibited cell shrinkage along with distortion of the normal cell morphology. Angustifoline-treated cells were also arrested in the G2/M phase of the cell cycle, showing strong dose-dependence. The compound also led to inhibition of cell migration and cell invasion. The results showed that treatment of these cells led to generation of autophagic cell vesicles. Furthermore, it was observed that the expression of Beclin-1 and LC3-II proteins was significantly upregulated in the angustifoline-administered COLO-205 cells. CONCLUSIONS: In brief, the present study hints towards the potent anticancer potential of the natural product angustifoline against COLO-205 human colon cancer cells with in depth mechanistic studies.",
              "Currently speaking, it is noted that radiofrequency ablation (RFA) has been the most widely used treatment for hepatocellular carcinoma (HCC) occurring in patients. However, accumulating evidence has demonstrated that the incidence of insufficient RFA (IRFA) may result in the identified rapid progression of residual HCC in the patient, which can greatly hinder the effectiveness and patient reported benefits of utilizing this technique. Although many efforts have been proposed, the underlying mechanisms triggering the rapid progression of residual HCC after IRFA have not yet been fully clarified through current research literature reviews. It was shown in this study that cell proliferation, migration and invasion of residual HepG2 and SMMC7721 cells were significantly increased after the IRFA was simulated in vitro. In other words, it is noted that IRFA could do this by enhancing the image of autophagy of the residual HCC cell via the HIF-1alpha/BNIP3 pathway. Consequently, the down-regulation of BNIP3 may result in the inhibition of the residual HCC cell progression and autophagy after IRFA. Our present study results suggest that IRFA could promote residual HCC cell progression in vitro by enhancing autophagy via the HIF-1alpha/BNIP3 pathway. For this reason, it is noted that the targeting of the BNIP3 may be useful in preventing the rapid growth and metastasis of residual HCC after IRFA. [BMB Reports 2019; 52(4): 277-282].",
              "The present study aimed to explore the repair effect and mechanism of bone marrow mesenchymal stem cells (BMSCs) transplantation on injured kidneys caused by hexavalent chromium (Cr (VI)). Wistar rats were intraperitoneally injected with 0.4mg/kg*bw Cr (VI) ion solution. After 30 days, 1x10(7) BMSCs were transplanted into rats. After cell transplantation for 2 weeks, there was no significant difference in the chromium content between the model and BMSCs-therapy group by atomic absorption spectrometry. In BMSCs-therapy group, the renal organ index, the serum levels of blood urea nitrogen (BUN) and creatinine (CRE), malonaldehyde (MDA) content were significantly decreased, superoxide dismutase (SOD) activity was significantly elevated, and the pathological changes were improved compared with the model group. The results of immunohistochemical and western blot assays showed that the expressions of apoptosis-related proteins Bax, Cytochrome c, and Caspase-3, as well as autophagy-associated proteins Beclin 1, PINK1, Parkin, p-Parkin, LC3B, and the MAPK signaling pathway, including the ratio of p-p38 to p38 and p-JNK to JNK were all significantly decreased, Bcl-2 and p62 expressions, and the ratio of p-ERK to ERK were significantly elevated in BMSCs-therapy group compared with the model group. These results suggested that BMSCs repaired Cr (VI)-injured kidney through decreasing mitochondria-mediated apoptosis and mitophagy mediated by downregulating phosphorylation of p38 and JNK, upregulating phosphorylation of ERK.",
              "OBJECTIVES: Thyroid carcinoma (TC) represents a malignant neoplasm affecting the thyroid. Current treatment strategies include the removal of part of the thyroid; however, this approach is associated with a significant risk of developing hypothyroidism. In order to adequately understand the expression profiles of TNRC6C-AS1 and STK4 and their potential functions in TC, an investigation into their involvement with Hippo signalling pathway and the mechanism by which they influence TC apoptosis and autophagy were conducted. METHODS: A microarray analysis was performed to screen differentially expressed lncRNAs associated with TC. TC cells were employed to evaluate the role of TNRC6C-AS1 by over-expression or silencing means. The interaction of TNRC6C-AS1 with methylation of STK4 promoter was evaluated to elucidate its ability to elicit autophagy, proliferation and apoptosis. RESULTS: TNRC6C-AS1 was up-regulated while STK4 was down-regulated, where methylation level was elevated. STK4 was verified as a target gene of TNRC6C-AS1, which was enriched by methyltransferase. Methyltransferase's binding to STK4 increased expression of its promoter. Over-expressed TNRC6C-AS1 inhibited STK4 by promoting STK4 methylation and reducing the total protein levels of MST1 and LATS1/2. The phosphorylation of YAP1 phosphorylation was decreased, which resulted in the promotion of SW579 cell proliferation and tumorigenicity. CONCLUSION: Based on our observations, we subsequently confirmed the anti-proliferative, pro-apoptotic and pro-autophagy capabilities of TNRC6C-AS1 through STK4 methylation via the Hippo signalling pathway in TC.",
              "Autophagy is an essential process for maintaining cellular homeostasis, a critical process in all cell types. Because neurons are post-mitotic cells, maintaining cellular and functional homeostasis is more important in neurons than in other types of cells. Synapses are fundamental units needed for neural communication, and synapses with consistent protein quality are essential for neural functionality. Dysregulation of autophagy in neurons has been shown to be related to neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. This review describes the role of autophagy in the maintenance of synaptic functionality and the association between synaptic autophagy and neurodegenerative diseases.",
              "Sepsis is characterized by life-threatening organ dysfunction caused by a deregulated host response to infection. Autophagy is one of the innate immune defense mechanisms against microbial attack. Previous studies have demonstrated that autophagy is activated initially in sepsis, followed by a subsequent phase of impairment. A number of sepsis-related studies have shown that autophagy plays a protective role in multiple organ injuries partly by clearing pathogens, regulating inflammation and metabolism, inhibiting apoptosis and suppressing immune reactions. In this review, we present a general overview of and recent advances in the role of autophagy in sepsis and consider the therapeutic potential of autophagy activators in treating sepsis.",
              "Adiponectin regulates metabolism through blood glucose control and fatty acid oxidation, partly mediated by downstream effects of adiponectin signaling in skeletal muscle. More recently, skeletal muscle has been identified as a source of adiponectin expression, fueling interest in the role of adiponectin as both a circulating adipokine and a locally expressed paracrine/autocrine factor. In addition to being metabolically responsive, skeletal muscle functional capacity, calcium handling, growth and maintenance, regenerative capacity, and susceptibility to chronic inflammation are all strongly influenced by adiponectin stimulation. Furthermore, physical exercise has clear links to adiponectin expression and circulating concentrations in healthy and diseased populations. Greater physical activity is generally related to higher adiponectin expression while lower adiponectin levels are found in inactive obese, pre-diabetic, and diabetic populations. Exercise training typically restores plasma adiponectin and is associated with improved insulin sensitivity. Thus, the role of adiponectin signaling in skeletal muscle has expanded beyond that of a metabolic regulator to include several aspects of skeletal muscle function and maintenance critical to muscle health, many of which are responsive to, and mediated by, physical exercise.",
              "We tested the hypothesis that Let-7d-3p contributes to cardiac cell protection during hypoxic challenge. Myoblast H9c2 cells and primary neonatal rat ventricular cardiomyocytes (NRVM) were transfected with five selected miRNA mimics. Both cell lines were subjected to 0.2% oxygen hypoxia. The protective effects of these miRNAs were determined by assessment of cell metabolic activity by CCK8 assay and measurement of lactate dehydrogenase (LDH) release as a marker of cell injury. Apoptosis and autophagy flux were assessed by Annexin V/7-AAD double staining and the ratio of LC3 II/I with Baf-A1 treatment, an autophagy flux inhibitor, respectively. Luciferase-reporter assay, RT-qPCR and Western blots were performed to identify the changes of relevant gene targets. Among five miRNA mimic transfections, Let-7d-3p increased CCK8 activity, and decreased LDH release in both H9c2 and NRVM during hypoxia. Apoptosis was significantly reduced in H9c2 cells transfected with Let-7d-3p mimic. Autophagy and autophagy flux were not affected. In silico, mRNAs of HMGA2, YY1, KLF9, KLF12, and MEX3C are predicted targets for Let-7d-3p. Luciferase-reporter assay confirmed that Let-7d-3p bound directly to the 3'-UTR region of HMGA2, MEX3C, and YY1, the down-regulations of these mRNAs were verified in both H9c2 and NRVM. The protein expression of HMGA2, but not others, was downregulated in H9c2 and NRVM. It is known that HMGA2 is a strong apoptosis trigger through the blocking of DNA repair. Thus, we speculate that the anti-apoptotic effects of Let-7d-3p mimic during hypoxia challenge are due to direct targeting of HMGA2.",
              "Autophagy serves as a cell survival mechanism which becomes dysregulated under pathological conditions and aging. Aortic valve thickening and calcification causing left ventricular outflow obstruction is known as calcific aortic valve stenosis (CAVS). CAVS is a chronic and progressive disease which increases in incidence and severity with age. Currently, no medical treatment exists for CAVS, and the role of autophagy in the disease remains largely unexplored. To further understand the role of autophagy in the progression of CAVS, we analyzed expression of key autophagy genes in healthy, thickened, and calcified valve tissue from 55 patients, and compared them with nine patients without significant CAVS, undergoing surgery for aortic regurgitation (AR). This revealed a upregulation in autophagy exclusively in the calcified tissue of CAVS patients. This difference in autophagy between CAVS and AR was explored by LC3 lipidation in valvular interstitial cells (VICs), revealing an upregulation in autophagic flux in CAVS patients. Inhibition of autophagy by bafilomycin-A1 led to a decrease in VIC survival. Finally, treatment of VICs with high phosphate led to an increase in autophagic activity. In conclusion, our data suggests that autophagy is upregulated in the calcified tissue of CAVS, serving as a compensatory and pro-survival mechanism.",
              "Ultraviolet (UV)-B radiation is a major environmental risk factor that is responsible for the development and progression of many skin cancers. Apigenin, a type of bioflavonoid, has been reported to inhibit UVB-induced skin cancer. However, how apigenin functions in keratinocytes with UV damage remains unclear. In this study, by lactate dehydrogenase (LDH) release assay, we found that apigenin treatment increased cell death in the primary human epidermal keratinocytes (HEKs) and the cutaneous squamous cell carcinoma cell line COLO-16. Apigenin treatment reduced microtubule-associated protein 1 light chain 3 (LC3)-II turnover, acridine orange staining and GFP-LC3 puncta in both cell types, suggesting autophagy inhibition. However, apigenin treatment restored the inhibition of autophagy in UVB-challenged HEKs. Moreover, apigenin treatment restored the UVB-induced downregulation of ataxia-telangiectasia mutated (ATM), ataxia-telangiectasia, Rad3-related (ATR) and the unfolded protein response (UPR) regulatory proteins, BiP, IRE1alpha and PERK in HEKs. Apigenin treatment also inhibited UVB-induced apoptosis and cell death in HEKs. In addition, autophagy inhibition by autophagy-related gene (ATG) 5 RNA interference interrupted apigenin-induced restoration of ATR, ATM and BiP, which were downregulated in HEKs exposed to UVB radiation. Our findings indicate that apigenin exhibits a novel protective effect in keratinocytes with UVB damage, suggesting potential application as a photoprotective agent.",
              "The development of low-frequency ultrasound imaging technology and the improvement of ultrasound contrast agent production technology mean that they play an increasingly important role in tumor therapy. The interaction between ultrasound and microbubbles and their biological effects can transfer and release microbubbles carrying genes and drugs to target tissues, mediate the apoptosis of tumor cells, and block the embolization of tumor microvasculature. With the optimization of ultrasound parameters, the development of targeted microbubbles, and the emergence of various composite probes with both diagnostic and therapeutic functions, low-frequency ultrasound combined with microbubble contrast agents will bring new hope for clinical tumor treatment.",
              "The tumor suppressor p53 plays essential roles in cellular protection mechanisms against a variety of stress stimuli and its activation induces apoptosis or autophagy in certain cancer cells. Here, we identified protopine, an isoquinoline alkaloid isolated from Nandina domestica, as an activator of the p53 pathway from cell-based natural compound screening based on p53-responsive transcription. Protopine increased the p53-mediated transcriptional activity and promoted p53 phosphorylation at the Ser15 residue, resulting in stabilization of p53 protein. Moreover, protopine up-regulated the expression of p21(WAF1/CIP1) and BAX, downstream genes of p53, and inhibited the proliferation of HCT116 colon cancer cells. Apoptosis was elicited by protopine as indicated by caspase-3/7 activation, poly ADP ribose polymerase cleavage, and increased population of Annexin V-FITC-positive cells. Furthermore, protopine induced the formation of microtubule-associated protein 1 light chain 3 (LC3) puncta and LC3-II turnover, typical biochemical markers of autophagy, in HCT116 cells. Our findings suggest that protopine exerts its antiproliferative activity by stimulating the p53 pathway and may have potential as a chemopreventive agent for human colon cancer.",
              "Embryonic stem cells (ESCs) can propagate in an undifferentiated state indefinitely in culture and retain the potential to differentiate into any somatic lineage as well as germ cells. The catabolic process autophagy has been reported to be involved in ESC identity regulation, but the underlying mechanism is still largely unknown. Here we show that EPG5, a eukaryotic-specific autophagy regulator which mediates autophagosome/lysosome fusion, is highly expressed in ESCs and contributes to ESC identity maintenance. We identify that the deubiquitinating enzyme USP8 binds to the Coiled-coil domain of EPG5. Mechanistically, USP8 directly removes non-classical K63-linked ubiquitin chains from EPG5 at Lysine 252, leading to enhanced interaction between EPG5 and LC3. We propose that deubiquitination of EPG5 by USP8 guards the autophagic flux in ESCs to maintain their stemness. This work uncovers a novel crosstalk pathway between ubiquitination and autophagy through USP8-EPG5 interaction to regulate the stemness of ESCs.",
              "Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease that threatens human health, and present therapies remain limited due to the lack of effective drugs. Lipid metabolic disturbance and oxidative stress have strong links to the development of NAFLD, while autophagy was generally accepted as a key regulatory mechanism on these steps. Our previous studies indicated that cherry anthocyanins (CACN) protected against high fat diet-induced obesity and NALFD in C57BL/6 mice, while the underlying molecule mechanism is still unclear. Thus, in this study, we show that CACN protect against oleic acid- (OA-) induced oxidative stress and attenuate lipid droplet accumulation in NAFLD cell models. According to the results of a transmission electron microscope (TEM), western blot, immunofluorescence (IF), and adenovirus transfection (Ad-mCherry-GFP-LC3B), autophagy is in accordance with the lipid-lowering effect induced by CACN. Further studies illustrate that CACN may activate autophagy via mTOR pathways. In addition, an autophagy inhibitor, 3-methyladenine (3-MA), was applied and the result suggested that autophagy indeed participates in the lipid clearance process in OA-induced lipid accumulation. All these results indicate that the positive effects of CACN on OA-induced hepatic lipid accumulation are mediated via activating autophagy, showing a potential target for the therapeutic strategy of NAFLD.",
              "Diabetes mellitus is a serious disease endangering human health worldwide. Vitamin D (Vit D) is a well-characterized regulator of calcium-phosphorus metabolism that also exerts other biological effects extending far beyond mineral homeostasis. Some epidemiological studies have suggested that Vit D has a role in defense against diabetes, although the mechanism remains unclear. Autophagy, an intracellular catabolic process, is necessary to maintain the normal structure and function of host cells. In our previous study, we found that Vit D could induce autophagy of pancreatic beta cells and prevent insulitis, although the underlying mechanisms remain to be fully elucidated. In this study, the protective effect of 1,25(OH)2D3, the physiologically active metabolite of Vit D, against streptozotocin-induced cytotoxicity in rat insulinoma cell line (INS-1) cells was explored. Cell viability and insulin secretion of INS-1 cells in response to different treatments were measured with a cell counting kit and enzyme-linked immune absorbent assay (ELISA), respectively. In addition, malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were measured by ELISA. RT-PCR and Western blot analyses were used to detect autophagy levels, reactive oxygen species (ROS) was assessed by fluorescence microscope, ultrastructure analysis was performed using transmission electron microscopy. The results demonstrated that 1,25(OH)2D3 could increase cell viability and insulin secretion of INS-1 cells, and protected cells from oxidative damage induced by streptozotocin (STZ) through autophagy activation. These findings shed light on mechanisms underlying the ameliorative effects of Vit D on diabetes mellitus.",
              "Objective: To investigate the effects of artesunate combined with bortezomib on the proliferation, apoptosis and autophagy of human acute myeloid leukemia cell lines MV4-11, and its mechanisms. Methods: MTT method was used to determine the anti-proliferation effect of different concentrations of artesunate, bortezomib and their combination on MV4-11 cells. The cell apoptosis were analyzed by flow cytometry. The expression of cleaved-Caspase-3, Bcl-2 family protein (Bcl-2, Mcl-1, Bim, Bax) and autophagy-related protein LC3B were assayed by Western blot. Results: Artesunate displayed a proliferation inhibition effect on MV4-11 with dose- and time-dependent manner, the IC(50) of artesunate on MV4-11 after 48 hours was 1.44 mug/ml. Bortezomib displayed a proliferation inhibition effect on MV4-11 with dose-dependent manner, the IC(50) of bortezomib on MV4-11 after 48 hours was 8.97 nmol/L. The combination of artesunate (0.75, 1.0 mug/ml) and Bortezomib (6, 8 nmol/L) showed higher inhibition on MV4-11 than artesunate or bortezomib alone in the same concentration gradient after 48 hours (P<0.05) . The cooperation index of the two drugs were all less than 1. The 48 h apoptotic rate of artesunate (1.5 mug/ml) on MV4-11 was (15.27+/-2.18) %, (19.85+/-3.23) % of bortezomib (8 nmol/L) , (81.67+/-5.96) % of combination of the two drugs, significantly higher than the single group (P<0.05) . When combination of the two drugs on MV4-11 after 24 hours, the levels of pro-apoptotic protein Bim and the cleaved activation of Caspase-3 and autophagy-related protein LC3B were up-regulated and the anti-apoptotic protein Bcl-2 expressions was down-regulated. Conclusion: Combination of artesunate with bortezomib shows a significant synergistic effects on proliferation, apoptosis and autophagy of MV4-11 cell lines, which may be associated with Bcl-2 family proteins expression.",
              "Obesity has many deleterious effects on the cardiovascular system, mediated by changes in insulin sensitivity, dyslipidaemia, oxidative stress and inflammation. Current therapies mainly focus on caloric intake suppression and bariatric surgery, however the efficacy of these approaches remains elusive as most patients regain their body weight within the next 5years. A better understanding of the pathophysiology of obesity is of paramount importance for the development of new therapeutic strategies to prevent vascular complications. Autophagy has emerged as key self-degrading process responsible for the maintenance of cellular homeostasis. Defects in autophagy homeostasis are implicated in metabolic disorders, including obesity, insulin resistance, diabetes mellitus and atherosclerosis. Most importantly, autophagy regulates animal lifespan. Albeit ample preclinical evidence supports the therapeutic promise of autophagy modulators for the treatment of obesity and metabolic diseases, the clinical efficacy of pharmacological modulation of autophagy remains to be proven. Recent work has shown that GLP-1-based therapeutic approaches may positively affect autophagy in perivascular adipose tissue, thus improving obesity-related endothelial dysfunction. In the present review we discuss current evidence on the role of autophagy in obesity, with a specific focus on DPP-4 inhibitors (DPP-4i) and GLP-1 receptor agonists (GLP-1 RA) as modulators of this process. Experimental evidence on GLP-1-based approaches is critically discussed in light of recent clinical trials with DPP-4i and GLP-1 RA.",
              "BACKGROUND: Autophagy, a process for degrading intracellular substances to maintain basal metabolic turnover, is known to be perturbed in gastric cancer. Programmed cell death-1 (PD-1) with its ligand (PD-L1) are important immune checkpoint proteins and their regulation by autophagy has been reported in mouse melanoma and human ovarian cancer. Here, we explored the interplay between autophagy and the PD1/PD-L1 axis in gastric cancer. METHODS: The expression of PD-L1 in gastric cancer cells was detected by Western blot and flow cytometry analysis. The effect of autophagy inhibition on PD-L1 expression was examined in vitro and in vivo. The molecular mechanisms of the regulation of PD-L1 by autophagy were evaluated in gastric cancer cell lines. The clinical relevance of autophagy-related markers p62/SQSTM1 and LC3 with PD-L1 was evaluated in 137 patients with gastric cancer. RESULTS: We found that inhibition of autophagy by pharmacological inhibitors or small interfering RNAs increased the levels of PD-L1 in cultured gastric cancer cells and in xenografts. Interferon (IFN)-gamma also promoted PD-L1 gene transcription, whose action was enhanced by autophagy inhibition. Mechanistically, autophagy inhibition led to the accumulation of p62/SQSTM1 and activation of nuclear factor (NF)-kappaB, in which NF-kappaB inhibition or p62/SQSTM1 knockdown attenuated PD-L1 induction by autophagy inhibition. Immunohistochemical staining of primary tumor tissues of 137 patients with gastric cancer showed that LC3 and p62/SQSTM1 protein levels were positively correlated with PD-L1 (LC3, p < 0.001; p62/SQSTM1, p < 0.05). The expression of PD-L1 was also positively correlated with tumor lymphocyte infiltration (p < 0.001). CONCLUSIONS: We discovered that autophagy regulates PD-L1 expression in gastric cancer through the p62/SQSTM1-NF-kappaB pathway. Pharmacological modulation of autophagy may thus influence the therapeutic efficacy of PD-L1 blockade in gastric cancer.",
              "Atherosclerosis and nonalcoholic fatty liver disease (NAFLD) are frequent causes of death in the Western countries. Recently, it has been shown that autophagy dysfunction plays an important role in the pathogenesis of both atherosclerosis and NAFLD; thus, activators of autophagy might be useful for novel therapeutic interventions. Trehalose-a naturally occuring disaccharide present in plants, bacteria, fungi, insects, and certain types of shrimps-is a known inducer of autophagy. However, according to the literature, its anti-atherosclerotic and anti-steatotic potential seem to depend on the experimental setting. The aim of our study was to comprehensively describe the influence of a prolonged treatment with orally administered trehalose on the development of atherosclerotic lesions and hepatic steatosis in apolipoprotein E knockout (apoE(-/-)) mice in an experimental set up reflecting both moderate and severe proatherogenic conditions: male apoE(-/-) mice on a chow diet (CD) and female apoE(-/-) mice fed with a high-fat diet (HFD). We found that exogenous trehalose inhibited atherosclerosis and attenuated hepatic steatosis in apoE(-/-) mice. Such effects of trehalose were not associated with changes of plasma cholesterol, low-density lipoproteins (LDL), or high-density lipoproteins (HDL). Moreover, the anti-steatotic action of trehalose in the liver was associated with the induction of autophagy. The exact molecular mechanisms of both the anti-atherosclerotic action of trehalose and its inhibitory effect on liver steatosis require further clarification.",
              "Human papillomavirus (HPV) infection is linked to several diseases, the most prominent of which are cervical cancer and genital condyloma acuminatum. Previous studies have suggested an effective role for 5-aminolevulinic acid photodynamic therapy (ALA-PDT) against various cancers by the induction of autophagy and apoptosis. However, few reports have focused on the effectiveness of ALA-PDT on HPV related disorders. To identify the role of ALA-PDT in the context of HPV infection, we initially investigated 111 patients suffering from genital condyloma acuminatum. HPV viral load detected before and after ALA-PDT treatment was compared during this procedure; a significant difference was noted. HeLa (HPV18) cells were exposed to ALA-PDT in vitro to further explore the underlying mechanisms. Western blot analysis showed that ALA-PDT induces LC3II and p62 expression, along with the up regulation of caspase-3 and cleaved caspase-3. Our study also demonstrated that ALA-PDT treatment inhibits the proliferation of HeLa cells in a dose dependent manner and effectively reduces HPV viral load via autophagy and apoptosis by regulating the Ras/Raf/MEK/ERK and PI3K/AKT/mTOR pathways. Hydroxychloroquine (HCQ), although it inhibited autophagy degradation, functioned to activate reactive oxygen species (ROS) levels of ALA-PDT to enhance the observed effect. These findings suggest strategies for the improvement of PDT efficacy in patients.",
              "A paradox of tumor immunology is that tumor-infiltrating lymphocytes are dysfunctional in situ, yet are capable of stem cell-like behavior including self-renewal, expansion, and multipotency, resulting in the eradication of large metastatic tumors. We find that the overabundance of potassium in the tumor microenvironment underlies this dichotomy, triggering suppression of T cell effector function while preserving stemness. High levels of extracellular potassium constrain T cell effector programs by limiting nutrient uptake, thereby inducing autophagy and reduction of histone acetylation at effector and exhaustion loci, which in turn produces CD8(+) T cells with improved in vivo persistence, multipotency, and tumor clearance. This mechanistic knowledge advances our understanding of T cell dysfunction and may lead to novel approaches that enable the development of enhanced T cell strategies for cancer immunotherapy.",
              "Recent studies suggest that phytochemicals, as part of the food matrix, might alter the toxicity of nanoparticles (NPs); however, relatively few studies have investigated the impact of anthocyanidins on the toxicity of NPs to cells lining the gastrointestinal tract. Therefore, this study used cyanidin chloride (CC) as the model for anthocyanidins and investigated the effects of CC on the toxicity of ZnO or Ag NPs to Caco-2cells. Exposure to ZnO but not Ag NPs significantly induced cytotoxicity. The presence of CC, but not its analog quercetin (Qu), modestly protected Caco-2cells from ZnO NP exposure. However, the intracellular superoxide, Zn ions, or release of interleukin-8 after ZnO NP exposure were not significantly affected by the presence of CC. Rather, CC promoted the expression of autophagic genes ATG5, ATG7, and BECN1 as well as the ratio of LC3-II/I after exposure to ZnO NPs. Meanwhile, the presence of autophagic inhibitors (chloroquine, NH4Cl, bafilomycin A1) significantly promoted the cytotoxicity of ZnO NPs and inhibited the cytoprotective effects of CC. In conclusion, these data suggest that CC could modestly protect Caco-2cells from ZnO NP exposure, probably through the induction of autophagy.",
              "BACKGROUND: Non-small cell lung cancer (NSCLC) is the most common type of lung cancer with a poor prognosis. We previously found that protein disulfide isomerase family 6 (PDIA6) is upregulated in lung squamous cell carcinoma (LSCC). This study aimed to elucidate the clinical relevance, biological functions, and molecular mechanisms of PDIA6 in NSCLC. METHODS: The expression of PDIA6 in NSCLC was assessed using the TCGA database, western blotting, and immunohistochemistry. Correlations of PDIA6 expression with clinicopathological and survival features were evaluated. The functions of PDIA6 in regulating NSCLC cell growth, apoptosis, and autophagy were investigated using gain-and loss-of-function strategies in vitro or in vivo. The underlying molecular mechanisms of PDIA6 function were examined by human phospho-kinase array and co-immunoprecipitation. FINDINGS: PDIA6 expression was upregulated in NSCLC compared with adjacent normal tissues, and the higher PDIA6 expression was correlated with poor prognosis. PDIA6 knockdown decreased NSCLC cell proliferation and increased cisplatin-induced intrinsic apoptosis, while PDIA6 overexpression had the opposite effects. In addition, PDIA6 regulated cisplatin-induced autophagy, and this contributed to PDIA6-mediated apoptosis in NSCLC cells. Mechanistically, PDIA6 reduced the phosphorylation levels of JNK and c-Jun. Moreover, PDIA6 interacted with MAP4K1 and inhibited its phosphorylation, ultimately inhibiting the JNK/c-Jun signaling pathway. INTERPRETATION: PDIA6 is overexpressed in NSCLC and inhibits cisplatin-induced NSCLC cell apoptosis and autophagy via the MAP4K1/JNK/c-Jun signaling pathway, suggesting that PDIA6 may serve as a biomarker and therapeutic target for NSCLC patients. FUND: National Natural Science Foundation of China and Institutions of higher learning of innovation team from Liaoning province.",
              "Background: The potential mechanism of postoperative cognitive impairment is still largely unclear. The activation of NLRP3 inflammasome had been reported to be involved in neurodegenerative diseases, including postoperative cognitive change, and is closely related to mitochondrial ROS and mitophagy. Honokiol (HNK) owns multiple organic protective effects. This study is aimed at observing the neuroprotective effect of HNK in postoperative cognitive change and examining the role of HNK in the regulation of mitophagy and the relationship between these effects and NLRP3 inflammasome activation in mice induced by surgery/anesthesia. Methods: In this study, mice were divided into several groups: control group, surgery group, surgery+HNK group, and surgery+HNK+3-methyladenine (3-MA) group. Hippocampal tissue samples were harvested and used for proinflammatory cytokines, mitochondrial ROS, and malondialdehyde (MDA) assay. The process of mitophagy and the activation of NLRP3 inflammasome were observed by Western blot, immunohistochemistry, and transmission electron microscopy. Results: The results showed that HNK treatment obviously recovered the postoperative decline and enhanced the expressions of LC3-II, Beclin-1, Parkin, and PINK1 at protein levels after surgery/sevoflurane treatment, which are both an autophagy marker and a mitophagy marker. In addition, HNK attenuated mitochondrial structure damage and reduced mtROS and MDA generation, which are closely associated with NLRP3 inflammasome activation. Honokiol-mediated mitophagy inhibited the activation of NLRP3 inflammasome and neuroinflammation in the hippocampus. Using 3-MA, an autophagy inhibitor, the neuroprotective effects of HNK on mitophagy and NLRP3 inflammasome activation were eliminated. Conclusion: These results indicated that HNK-mediated mitophagy ameliorates postoperative cognitive impairment induced by surgery/sevoflurane. This neuroprotective effect may be involved in inhibiting the activation of NLRP3 inflammasome and suppressing inflammatory responses in the hippocampus.",
              "BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a multifactorial fatal motoneuron disease without a cure. Ten percent of ALS cases can be pointed to a clear genetic cause, while the remaining 90% is classified as sporadic. Our study was aimed to uncover new connections within the ALS network through a bioinformatic approach, by which we identified C13orf18, recently named Pacer, as a new component of the autophagic machinery and potentially involved in ALS pathogenesis. METHODS: Initially, we identified Pacer using a network-based bioinformatic analysis. Expression of Pacer was then investigated in vivo using spinal cord tissue from two ALS mouse models (SOD1(G93A) and TDP43(A315T)) and sporadic ALS patients. Mechanistic studies were performed in cell culture using the mouse motoneuron cell line NSC34. Loss of function of Pacer was achieved by knockdown using short-hairpin constructs. The effect of Pacer repression was investigated in the context of autophagy, SOD1 aggregation, and neuronal death. RESULTS: Using an unbiased network-based approach, we integrated all available ALS data to identify new functional interactions involved in ALS pathogenesis. We found that Pacer associates to an ALS-specific subnetwork composed of components of the autophagy pathway, one of the main cellular processes affected in the disease. Interestingly, we found that Pacer levels are significantly reduced in spinal cord tissue from sporadic ALS patients and in tissues from two ALS mouse models. In vitro, Pacer deficiency lead to impaired autophagy and accumulation of ALS-associated protein aggregates, which correlated with the induction of cell death. CONCLUSIONS: This study, therefore, identifies Pacer as a new regulator of proteostasis associated with ALS pathology.",
              "Telomerase has cellular functions beyond telomere stabilization, including a role in mitochondria. The function of the catalytic component-TERT-in mitochondria is still unknown, but it seems to play a role in the response to oxidative stress. Here, we interrogated the role of the subcellular localization of TERT to the response to hydrogen peroxide (H(2)O(2)) treatment. Using normal human fibroblasts (NHF) expressing non-tagged wild type (WT) human TERT (hTERT) or nuclear localization and function (nuchTERT), a mutant that we previously described as being competent in telomere elongation, while not being able to localize to mitochondria, we found the differential activation of autophagy as a function of hTERT's subcellular localization. Specifically, we found that only cells expressing the mutant had significant increases in autophagy markers as a response to H(2)O(2) challenge. Either the reintroduction of the mitochondrial pool of hTERT or the expression of mitochondrially-targeted catalase in mutant cells blunted the autophagic response under oxidative stress. Interestingly, autophagy activation was also associated with decreased levels of mitochondrial DNA damage. Taken together, these results suggest that the loss of hTERT in mitochondria initiates a signaling cascade that allows for cells to adapt to and cope with the lack of mitochondrial telomerase. Such effects also influence the cellular response to oxidative damage.",
              "OBJECTIVE: To investigate the effects of autophagy on N-methy-D-aspartate (NMDA) receptor and its subunit NR2B and behavioral test in a rat model of neuropathic pain (NP). METHODS: Male Sprague-Dawley (SD) rats were divided into sham group, NP group, autophagy inhibitor 3-methyladenine (3-MA) pretreatment group (3-MA+NP group) and autophagy inducer rapamyein (Rap) group (Rap+NP group) by random number table with 22 rats in each group. NP animal model was reproduced by ligating sciatic nerve, while sciatic nerve of the rats in the sham group were only exposed but not ligated. The rats in two pretreatment groups were intraperitoneally challenged with 3-MA 15 mg/kg or Rap 10 mg/kg injection 1 hour before operation. Mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured before and 1, 3, 7, 14 days after operation in each group. Spinal cord tissues were harvested at 1 day and 7 days after operation for autophagosome observation by electron microscope. The expressions of autophagy protein microtubule-associated protein 1 light chain 3-II (LC3-II), Beclin1, and NMDA, NR2B were determined by Western Blot. The positive expression of LC3 was detected by immunofluorescence. RESULTS: Compared with sham group, the MWT and TWL of rats in NP group were decreased gradually with the prolongation of operation time, the number of autophagosome, the expressions of LC3-II, Beclin1, NMDA, NR2B, and the positive expression of LC3 in spinal cord were significantly increased at 1 day after operation and till 7 days, which indicated that NP led to hyperpathia and autophagy activation. Compared with NP group, MWT was significantly further decreased, TWL was further shortened, the number of autophagosome was decreased, the expressions of LC3-II and Beclin1 in spinal cord were decreased, and NMDA and NR2B expressions were further increased after 3-MA pretreatment, with significant differences at 1 day after operation [MWT (g): 29.4+/-2.4 vs. 42.5+/-6.6, TWL (s): 7.2+/-1.0 vs. 8.8+/-1.1, LC3-II/beta-actin: 0.38+/-0.03 vs. 0.52+/-0.07, Beclin1/beta-actin: 0.29+/-0.06 vs. 0.59+/-0.05, NMDA/beta-actin: 0.62+/-0.06 vs. 0.50+/-0.06, NR2B/beta-actin: 0.57+/-0.03 vs. 0.46+/-0.03, all P < 0.05]. Immunofluorescence staining confirmed that the positive expression of LC3 was significantly decreased. Rap pretreatment could increase MWT, TWL and the number of autophagosome, increase LC3-II and Beclin1 expressions in spinal cord, and decrease NMDA and NR2B expressions in NP rats, and significant differences at 1 day after operation were found as compared with those in NP group [MWT (g): 49.4+/-4.4 vs. 42.5+/-6.6, TWL (s): 10.5+/-1.2 vs. 8.8+/-1.1, LC3-II/beta-actin: 0.67+/-0.09 vs. 0.52+/-0.07, Beclin1/beta-actin: 0.71+/-0.08 vs. 0.59+/-0.05, NMDA/beta-actin: 0.40+/-0.05 vs. 0.50+/-0.06, NR2B/beta-actin: 0.34+/-0.04 vs. 0.46+/-0.03, all P < 0.05], and immunofluorescence showed that the positive expression of LC3 was increased and lasted for 7 days. It indicated that Rap could increase the activity of autophagy, alleviate the occurrence of hyperalgesia, and reduce the expressions of NMDA receptor and its NR2B subunit. CONCLUSIONS: NP could regulate the variety of NMDA/NR2B and hyperalgesia via increasing autophagy.",
              "OBJECTIVE: To investigate the relationship between different tidal volume (VT) mechanical ventilation (MV) and autophagy and mitochondrial damage in rats. METHODS: A total of 120 clean-grade male Sprague-Dawley (SD) rats were divided into five groups (n = 24) by random number table method, and then given 0 (spontaneous breathing), 10, 20, 30, 40 mL/kg VT for MV. The rats in each group were subdivided into four subgroups of 1, 2, 3, and 4 hours according to ventilation time, with 6 rats in each subgroup. The lung tissue and bronchoalveolar lavage fluid (BALF) were harvested, and alveolar macrophages (AMs) and type II alveolar epithelial cells (AEC II) were cultured in vitro. The mRNA and protein expressions of autophagy-associated protein microtubule-associated protein 1 light chain 3B-II (LC3B-II) and autophagy-related genes Beclin1 and p62 were determined by reverse transcription-polymerase chain reaction (RT-PCR) or Western Blot. Lung autophagosome formation was observed under transmission electron microscope. The levels of adenosine triphosphate (ATP), reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) in lung tissue were determined for assessing mitochondrial damage. RESULTS: There were no significant differences in the mRNA and protein expressions of LC3B-II, p62 and Beclin1 at 1 hour after ventilation among the groups. With the prolonged ventilation time, the mRNA and protein expressions of LC3B-II, p62 and Beclin1 in MV groups were increased gradually, peaked at 2-3 hours, and they were increased significantly in 30 mL/kg VT group as compared with those in spontaneous respiration group with statistical significances [ventilation for 2 hours: LC3B-II mRNA (2(-DeltaDeltaCt)) was 2.44+/-0.24 vs. 1.12+/-0.04, LC3B-II/LC3B-I was 1.42+/-0.16 vs. 0.57+/-0.03, p62 mRNA (2(-DeltaDeltaCt)) was 2.96+/-0.14 vs. 1.14+/-0.02, Beclin1 mRNA (2(-DeltaDeltaCt)) was 2.80+/-0.13 vs. 1.14+/-0.02; ventilation for 3 hours: p62/beta-actin was 1.14+/-0.15 vs. 0.55+/-0.04, Beclin1/beta-actin was 1.27+/-0.06 vs. 0.87+/-0.04, all P < 0.05]. Autophagosomes and autolysosomes were found in AEC II after ventilation for 2 hours at 30 mL/kg VT by transmission electron microscopy, but not in AEC I. Compared with spontaneous breathing group, ATP synthesis in AMs was significantly decreased at 2 hours of ventilation in 30 mL/kg VT group (A value: 0.82+/-0.05 vs. 1.00+/-0.00, P < 0.05), ROS accumulate in AMs and AEC II were significantly increased [ROS in AMs: (33.83+/-4.00)% vs. (6.90+/-0.62)%, ROS in AEC II: (80.68+/-0.90)% vs. (2.16+/-0.19)%, both P < 0.05]. With the increase in VT and the prolongation of ventilation time, ATP and ROS levels in AMs and AEC II were gradually decreased, the ATP (A value) in AMs at 4 hours of ventilation in 40 mL/kg VT group was 0.41+/-0.05, the ROS in AMs was (12.95+/-0.88)%, and the ROS in AEC II was (40.43+/-2.29)%. With the increase in VT and the prolongation of ventilation time, MMP levels were gradually increased, the MMP (green/red fluorescence intensity ratio) in AMs at 2 hours of ventilation in 30 mL/kg VT group was 1.11+/-0.17, the MMP in AEC II was 0.96+/-0.04, and the MMP (green/red fluorescence intensity ratio) at 4 hours of ventilation in 40 mL/kg VT group was 0.51+/-0.07 and 0.49+/-0.06, respectively. CONCLUSIONS: The MV with high VT could induce autophagy activation and mitochondrial damage in lung tissue of rats, and the longer the ventilation time, the more obvious autophagy in the lung.",
              "T-cell lymphomas are a heterogeneous group of cancers with different pathogenesis and poor prognosis. Histone deacetylases (HDACs) are epigenetic modifiers that modulate many key biological processes. In recent years, HDACs have been fully investigated for their roles and potential as drug targets in T-cell lymphomas. In this review, we have deciphered the modes of action of HDACs, HDAC inhibitors as single agents, and HDACs guided combination therapies in T-cell lymphomas. The overview of HDACs on the stage of T-cell lymphomas, and HDACs guided therapies both as single agents and combination regimens endow great opportunities for the cure of T-cell lymphomas.",
              "When mice are subjected to 60% calorie restriction for several days, they lose nearly all of their body fat. Although the animals lack energy stores, their livers produce enough glucose to maintain blood glucose at viable levels even after a 23-hour fast. This adaptation is mediated by a marked increase in plasma growth hormone (GH), which is elicited by an increase in plasma ghrelin, a GH secretagogue. In the absence of ghrelin, calorie-restricted mice develop hypoglycemia, owing to diminished glucose production. To determine the site of GH action, in the current study we used CRISPR/Cas9 and Cre recombinase technology to produce mice that lack GH receptors selectively in liver (L-Ghr (-/-) mice) or in adipose tissue (Fat-Ghr(-/-) mice). When subjected to calorie restriction and then fasted for 23 hours, the L-Ghr (-/-) mice, but not the Fat-Ghr(-/-) mice, developed hypoglycemia. The fall in blood glucose in L-Ghr(-/-) mice was correlated with a profound drop in hepatic triglycerides. Hypoglycemia was prevented by injection of lactate or octanoate, two sources of energy to support gluconeogenesis. Electron microscopy revealed extensive autophagy in livers of calorie-restricted control mice but not in L-Ghr (-/-) mice. We conclude that GH acts through its receptor in the liver to activate autophagy, preserve triglycerides, enhance gluconeogenesis, and prevent hypoglycemia in calorie-restricted mice, a model of famine.",
              "Enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD), which has high morbidity and mortality. It mainly threatens children under six years of age. Because of a poor understanding of its pathogenesis, there are no effective drugs to control EV71 infection. Previous studies showed that EV71 infection induced autophagy and the production of cytokine IL-6. However, the underlying mechanisms between autophagy and the production of IL-6 induced by EV71 remain unclear. This study aimed to reveal the regulatory mechanisms between autophagy and the expression of IL-6 induced by EV71 infection. Our results showed that the proliferation of human gastric epithelial (GES-1) cells was inhibited by EV71 in a time- and dose-dependent manner. In addition, EV71 induced autophagy in GES-1cells. EV71 infection promoted the expression and the release of IL-6 to the extracellular space, although the expression and release were inhibited by autophagy inhibitors 3-methyladenine (3-MA) and chloroquine (CQ) in GES-1cells. The phosphorylated levels of p38MAPK and ERK proteins in GES-1cells also increased after infection with EV71, and these changes were also reversed by 3-MA and CQ treatment. Our findings suggested that EV71-induced autophagy regulated the production of IL-6 through the p38MAPK and ERK signaling pathways.",
              "MicroRNAs (miRNAs) are short (20-23 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. In recent years, deep sequencing of the transcription is being increasingly utilized with the promise of higher sensitivity for the identification of differential expression patterns as well as the opportunity to discover new transcripts, including new alternative isoforms and miRNAs. In this study, miRNAs from A549 cells treated with/without rapamycin or starvation were subject to genome-wide deep sequencing. A total of 1534 miRNAs were detected from the rapamycin- and starvation-treated A549 cells. Among them, 31 miRNAs were consistently upregulated and 131 miRNAs were downregulated in the treated cells when compared with the untreated cells. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of the predicted target genes of the most significantly differentially expressed miRNAs revealed that the autophagy-related miRNAs are involved in cancer pathway. Taken together, our findings indicate that the underlying mechanism responsible for autophagy is associated with dysregulation of miRNAs in rapamycin- or starvation-induced A549 cells.",
              "Monitoring autophagy can provide valuable insights into understanding human pathological mechanisms, developing novel drugs, and exploring autophagy control approaches. Here, we proposed a new strategy to specifically monitor autophagy by lighting up the G-quadruplex structures entering autolysosomes. Based on this strategy, we designed a small-molecule fluorescent probe for autophagy imaging. This work not only opens up a new way for developing autophagy probes, but also provides an effective tool for autophagy research.",
              "AIMS: Parkinson's disease (PD) is a common neurodegenerative disease typically associated with the accumulation of alpha-synuclein. Autophagy impairment is thought to be involved in the dopaminergic neurodegeneration in PD. We investigate the effect of Apelin-36 on the activated phosphatidylinositol 3-kinase (PI3K)/protein kinase B(Akt)/the mammalian target of rapamycin (mTOR) autophagy pathway in 1-methyl-4-phenylpyridinium (MPP(+))-treated SH-SY5Y cells, which is involved in the cytoprotective effect of Apelin-36. MAIN METHODS: SH-SY5Y cells were treated with 1-Methyl-4-phenylpyridine (MPP(+)) with or without Apelin-36. The cell viability, apoptotic ratio, the form of autophagic vacuoles, the expression of tyrosine hydroxylase (TH), alpha-synuclein, phosphorylation of PI3K, AKT, mTOR, microtubule-associated protein 1 Light Chain 3 II/I (LC3II/I) and p62 were detected to investigate the neuroprotective effect of Apelin-36. KEY FINDINGS: The results indicate that Apelin-36 significantly improved the cell viability and decreased the apoptosis in MPP(+)-treated SH-SY5Y cells. The decreased expression of tyrosine hydroxylase (TH) induced by MPP(+) was significantly increased by Apelin36 pretreatment. Moreover, Apelin36 significantly increased the autophagic vacuoles. The ratio of LC3II/I was significantly increased by Apelin36, as well as the decreased p62 expression. In addition, the activated PI3K/AKT/mTOR pathway induced by MPP(+) was significantly inhibited by Apelin36. Additionally, Apelin36 significantly decreased the alpha-synuclein expression. Furthermore, the cytoprotective effect of Apelin-36 was weakened by pretreatment with Insulin-like Growth Factor-1 (IGF-1), an activator of PI3K/Akt, and MHY1485, an mTOR activator. SIGNIFICANCE: Our results demonstrated that Apelin-36 protects against MPP(+)-induced cytotoxicity through PI3K/Akt/mTOR autophagy pathway in PD model in vitro, which provides a new theoretical basis for the treatment of PD.",
              "Ovarian cancer is one the prevalent cancers in women and is responsible for 5% of all the cancer related mortalities in women. Owing to late diagnosis, frequent relapses, side effects of chemotherapy, development of drug resistance, there is pressing need to screen out novel and effective treatment options. Accumulating evidences suggest that miRNAs may prove essential therapeutic targets for the treatment of cancer. This study was designed to investigate the role and therapeutic potential of miR-34 in ovarian cancer. It was found that miR-34 is significantly downregulated in ovarian cancer cell lines. Overexpression of miR-34 causes significant decrease in the proliferation of OVACAR-3 ovarian cancer cells via activation of apoptosis and autophagy. The miR-34 overexpression was concomitant with upsurge of apoptosis related proteins (Bax) and the autophagy associated protein (LC3 II and p62). TargetScan analysis showed Notch 1 to be the main target of miR-34 in OVACAR-3cells which was further validated by luciferase reporter assay. The qRT-PCR results showed Notch 1 to be 3.2-4.1 fold higher in the ovarian cancer cell lines relative to the non-cancerous cells. Nonetheless, miR-34 overexpression in OVACAR-3cells resulted in the post-transcriptional suppression of Notch 1 expression. Silencing of Notch 1 also caused inhibition of OVACAR-3cell proliferation via induction of apoptosis and autophagy. Overexpression of Notch 1 could partially rescue the effects of miR-34 overexpression on the proliferation of OVACAR-3cells. Moreover, overexpression of miR-34 causes significant inhibition of the invasion of the OVACAR-3cells. The findings of the present study indicate the tumor suppressive role of miR-34 in ovarian cancer and may therefore prove to be a potential therapeutic target.",
              "Sun or therapy-related ultraviolet B (UVB) irradiation induces different cell death modalities such as apoptosis, necrosis/necroptosis and autophagy. Understanding of mechanisms implicated in regulation and execution of cell death program is imperative for prevention and treatment of skin diseases. An essential component of death-inducing complex is Fas-associated protein with death domain (FADD), involved in conduction of death signals of different death modalities. The purpose of this study was to enlighten the role of FADD in the selection of cell death mode after narrow-band UVB (NB-UVB) irradiation using specific cell death inhibitors (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethylketone (zVAD-fmk), Necrostatin-1 and 3-Methyladenine) and FADD-deficient (FADD(-/-)) mouse embryonic fibroblasts (MEFs) and their wild type (wt) counterparts. The results imply that lack of FADD sensitized MEFs to induction of receptor-interacting protein 1 (RIPK1)-dependent apoptosis by the generation of reactive oxygen species (ROS), but without activation of the proteins p53, Bax and Bcl-2 as well as without the enrolment of calpain-2. Autophagy was established as a contributing factor to NB-UVB-induced death execution. By contrast, wt cells triggered intrinsic apoptotic pathway that was resistant to the inhibition by zVAD-fmk and Necrostatin-1 pointing to the mechanism overcoming the cell survival. These findings support the role of FADD in prevention of autophagy-dependent apoptosis.",
              "Primary acquired nasolacrimal duct obstruction (PANDO) is a syndrome of unknown etiology, predominantly affecting post-menopausal females, characterized by progressive inflammation, fibrosis and subsequent obstruction of the nasolacrimal duct. Numerous factors have been proposed as possible etiologic factors and include anatomical configuration, ocular and nasal infections, peri-lacrimal vascular disorders, hormonal influence, lacrimal drainage lymphoid tissue, gastroesophageal reflux disease, topical medications, swimming pool exposure, smoking, genetic factors, autonomic and lysosomal dysregulation. The authors hypothesize Prolactin (PRL) and Prolactin-inducible protein (PIP) play a role in the etiopathogenesis of primary acquired nasolacrimal duct obstruction.",
              "BACKGROUND: Macroautophagy (hereafter autophagy) is a tightly regulated process that delivers cellular components to lysosomes for degradation. Damage-regulated autophagy modulator 1 (DRAM1) induces autophagy and is necessary for p53-mediated apoptosis. However, the signalling pathways regulated by DRAM1 are not fully understood. METHODS: HEK293T cells were transfected with FLAG-DRAM1 plasmid. Autophagic proteins (LC3 and p62), phosphorylated p53 and the phosphorylated proteins of the class I PI3K-Akt-mTOR-ribosomal protein S6 (rpS6) signalling pathway were detected with Western blot analysis. Cellular distribution of DRAM1 was determined with immunostaining. DRAM1 was knocked down in HEK293T cells using siRNA oligos which is confirmed by quantitative RT-PCR. Cells were serum starved for 18 h after overexpression or knockdown of DRAM1 to decrease the rpS6 activity to the basal level, and then the cells were stimulated with insulin growth factor, epidermal growth factor or serum. rpS6 phosphorylation and rpS6 were detected with Western blotting. Similarly, after overexpression or knockdown of DRAM1, phosphorylation of IGF-1Rbeta and IGF-1R were examined with Western blotting. Cell viability was determined with CCK-8 assay and colony formation assay. Finally, human cancer cells Hela, SW480, and HCT116 were transfected with the FLAG-DRAM1 plasmid and phosphorylated rpS6 and rpS6 were detected with Western blot analysis. RESULTS: DRAM1 induced autophagy and inhibited rpS6 phosphorylation in an mTORC1-dependent manner in HEK293T cells. DRAM1 didn't affect the phosphorylated and total levels of p53. Furthermore, DRAM1 inhibited the activation of the PI3K-Akt pathway stimulated with growth factors or serum. DRAM1 was localized at the plasma membrane and regulate the phosphorylation of IGF-1 receptor. DRAM1 decreased cell viability and colony numbers upon serum starvation. Additionally, DRAM1 inhibited rpS6 phosphorylation in several human cancer cells. CONCLUSIONS: Here we provided evidence that DRAM1 inhibited rpS6 phosphorylation in multiple cell types. DRAM1 inhibited the phosphorylation of Akt and the activation of Akt-rpS6 pathway stimulated with growth factors and serum. Furthermore, DRAM1 regulated the activation of IGF-1 receptor. Thus, our results identify that the class I PI3K-Akt-rpS6 pathway is regulated by DRAM1 and may provide new insight into the potential role of DRAM1 in human cancers.",
              "Crotonaldehyde is an extremely toxic alpha,beta-unsaturated aldehyde found in cigarette smoke, and it causes inflammation and vascular dysfunction. Autophagy has been reported to play a key role in the pathogenesis of vascular diseases. However, the precise mechanism underlying the role of acute exposure crotonaldehyde in vascular disease development remains unclear. In the present study, we aimed to investigate the effect of crotonaldehyde-induced autophagy in endothelial cells. Acute exposure to crotonaldehyde decreased cell viability and induced autophagy followed by cell death. In addition, inhibiting the autophagic flux markedly promoted the viability of endothelial cells exposed to high concentrations of crotonaldehyde. Crotonaldehyde activated the AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (MAPK) pathways, and pretreatment with inhibitors specific to these kinases showed autophagy inhibition and partial improvement in cell viability. These data show that acute exposure to high concentrations of crotonaldehyde induces autophagy-mediated cell death. These results might be helpful to elucidate the mechanisms underlying crotonaldehyde toxicity in the vascular system and contribute to environmental risk assessment.",
              "BACKGROUND: Chinese herbal medicines (CHMs) are a resource of natural compounds (ingredients) and their potential chemical derivatives with anticancer properties, some of which are already in clinical use. Bei-Mu (BM), Jie-Geng (JG), and Mai-Men-Dong-Tang (MMDT) are important CHMs prescribed for patients with lung cancer that have improved the survival rate. HYPOTHESIS/PURPOSE: The aim of this study was to systemically investigate the mechanisms of action of these CHM products in lung cancer cells. METHODS: We used a network pharmacology approach to study CHM product-related natural compounds and their lung cancer targets. In addition, the underlying anti-lung cancer effects of the natural compounds on apoptosis, cell cycle progression, autophagy, and the expression of related proteins was investigated in vitro. RESULTS: Ingredient-lung cancer target network analysis identified 20 natural compounds. Three of these compounds, ursolic acid, 2-(3R)-8,8-dimethyl-3,4-dihydro-2H-pyrano(6,5-f)chromen-3-yl)-5-methoxyphenol, and licochalcone A, inhibited the proliferation of A549 lung cancer cells in a dose-dependent manner. Signal pathway analyses suggested that these three ingredients may target cellular apoptosis, anti-apoptosis, and cell cycle-related proteins. These three ingredients induced apoptosis through the regulation of the expression of apoptotic and anti-apoptotic proteins, including B-cell lymphoma-2 and full-length and cleaved poly(ADP-ribose) polymerase proteins. They also induced cell cycle arrest in S and G2/M phases and autophagy in A549 cells. CONCLUSION: The pharmacological mechanisms of ingredients from MMDT on lung cancer may be strongly associated with their modulatory effects on apoptosis, autophagy, cell cycle progression, and cell proliferation.",
              "Autophagy is a highly conserved mechanism to overcome various stresses and recycle cytoplasmic components and organelles. Ubiquitin-like (UBL) protein Atg12 is a key protein involved in autophagosome formation through stimulation of Atg8 conjugation to phosphatidylethanolamine. Here, we describe the identification of the autophagy-related gene Acatg12, encoding an Atg12 homologous protein in the cephalosporin C producing fungus Acremonium chrysogenum. Disruption of Acatg12 impaired the delivery and degradation of eGFP-Atg8, indicating that the autophagic process was blocked. Meanwhile, conidiation was dramatically reduced in the Acatg12 disruption mutant (Acatg12). In contrast, cephalosporin C production was increased twofold in Acatg12, but fungal growth was reduced after 6 days fermentation. Consistent with these results, the transcriptional level of the cephalosporin biosynthetic genes was increased in Acatg12. The results extend our understanding of autophagy in filamentous fungi.",
              "Macroautophagy is an evolutionarily conserved cellular maintenance program, meant to protect the brain from premature aging and neurodegeneration. How neuronal autophagy, usually loosing efficacy with age, intersects with neuronal processes mediating brain maintenance remains to be explored. Here, we show that impairing autophagy in the Drosophila learning center (mushroom body, MB) but not in other brain regions triggered changes normally restricted to aged brains: impaired associative olfactory memory as well as a brain-wide ultrastructural increase of presynaptic active zones (metaplasticity), a state non-compatible with memory formation. Mechanistically, decreasing autophagy within the MBs reduced expression of an NPY-family neuropeptide, and interfering with autocrine NPY signaling of the MBs provoked similar brain-wide metaplastic changes. Our results in an exemplary fashion show that autophagy-regulated signaling emanating from a higher brain integration center can execute high-level control over other brain regions to steer life-strategy decisions such as whether or not to form memories.",
              "OBJECTIVE: In this study, we aim to evaluate Toll-like receptor 2 (TLR2) expression in human glioma tumors and the correlation between its expression with degrees of malignancy and autophagy, development of tumors. METHOD: Immunohistochemistry and Western blot were carried out to determine the expression of LC3, Beclin1 and TLR2 in 74 glioma specimens. We analyzed the prognosis of 551 glioma patients through the Cancer Genome Atlas (TCGA). To determine the effect of TLR2 in glioma, we manipulated TLR2 expression using TLR2 plasmid transfer technique in U87 human glioma cell. RESULTS: TLR2 expression in high-grade was significantly higher than that in low-grade glioma group (P<0.05). TLR2 was positively correlated with tumor grade (P<0.05). Spearman correlation showed that the expression of TLR2 was positively correlated with the numbers of LC3 and Beclin1 (P<0.05). The patients with high TLR2 expression had a poorer outcome compared with the patients with low TLR2 in low-grade glioma (P<0.05). TLR2 overexpression enhances glioma cell activity and accelerates cell cycle progression. In addition, treatment with TLR2 overexpression increases the conversion rate of LC3-I to LC3-II and enhances the level of phosphorylated p38. CONCLUSION: TLR2 promotes development and progression of human glioma via enhancing autophagy.",
              "BACKGROUND: The vast majority of mitochondrial disorders have limited the clinical management to palliative care. Rapamycin has emerged as a potential therapeutic drug for mitochondrial diseases since it has shown therapeutic benefits in a few mouse models of mitochondrial disorders. However, the underlying therapeutic mechanism is unclear, the minimal effective dose needs to be defined and whether this therapy can be generally used is unknown. METHODS: We have evaluated whether low and high doses of rapamycin administration may result in therapeutic effects in a mouse model (Coq9(R239X)) of mitochondrial encephalopathy due to CoQ deficiency. The evaluation involved phenotypic, molecular, image (histopathology and MRI), metabolomics, transcriptomics and bioenergetics analyses. FINDINGS: Low dose of rapamycin induces metabolic changes in liver and transcriptomics modifications in midbrain. The high dose of rapamycin induces further changes in the transcriptomics profile in midbrain due to the general inhibition of mTORC1. However, neither low nor high dose of rapamycin were able to improve the mitochondrial bioenergetics, the brain injuries and the phenotypic characteristics of Coq9(R239X) mice, resulting in the lack of efficacy for increasing the survival. INTERPRETATION: These results may be due to the lack of microgliosis-derived neuroinflammation, the limitation to induce autophagy, or the need of a functional CoQ-junction. Therefore, the translation of rapamycin therapy into the clinic for patients with mitochondrial disorders requires, at least, the consideration of the particularities of each mitochondrial disease. FUND: Supported by the grants from \"Fundacion Isabel Gemio - Federacion Espanola de Enfermedades Neuromusculares - Federacion FEDER\" (TSR-1), the NIH (P01HD080642) and the ERC (Stg-337327)."
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"
         },
         "metadata": {},
         "output_type": "display_data"
        },
        {
         "name": "stdout",
         "output_type": "stream",
         "text": "\n已结束\n请稍等\n"
        },
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                "band": 1,
                "scale": "xscale"
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               "fillOpacity": [
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                 "test": "datum === tooltip",
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                "scale": "xscale",
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         "metadata": {},
         "output_type": "display_data"
        },
        {
         "name": "stdout",
         "output_type": "stream",
         "text": "\n已结束\n请稍等\n"
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"
         },
         "metadata": {},
         "output_type": "display_data"
        },
        {
         "name": "stdout",
         "output_type": "stream",
         "text": "\n已结束\n"
        }
       ]
      }
     },
     "c0f044f68f7b4e8c866ef6251b1a6e71": {
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     },
     "c779f019e04a42a98346feddfa40ff47": {
      "model_module": "@jupyter-widgets/controls",
      "model_module_version": "1.5.0",
      "model_name": "ButtonModel",
      "state": {
       "description": "点击按钮运行",
       "layout": "IPY_MODEL_f44f661ea867408693fc1a72d51e094e",
       "style": "IPY_MODEL_9372775f56ff4e7c9d980ba8b9cf1e45"
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      "model_module": "@jupyter-widgets/controls",
      "model_module_version": "1.5.0",
      "model_name": "BoundedIntTextModel",
      "state": {
       "description": "数量:",
       "layout": "IPY_MODEL_025893cc883c4eab9c6f4ffd1c074bf5",
       "max": 999999,
       "min": 1,
       "style": "IPY_MODEL_30558947d06e4d68889f9b5b24e84af0",
       "value": 300
      }
     },
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      "model_name": "LayoutModel",
      "state": {}
     }
    },
    "version_major": 2,
    "version_minor": 0
   }
  }
 },
 "nbformat": 4,
 "nbformat_minor": 4
}